Effect of organic acid, biofertilizers and two mycorrhiza species in the rootstock on some germination characteristics and seedling growth of Iranian ox-tongue (Echium amoenum Fisch & Mey.)

Document Type : Original Article

Authors

1 Assistant Professor, University of Gonabad

2 Professor, Ferdowsi University of Mashhad

3 Associate Professor, Ferdowsi University of Mashhad

Abstract

An experiment based on CRD design with three replications was conducted in 2014 year, in Ferdowsi University of Mashhad, Iran. The experimental treatments included 7 types of eco-friendly inputs: Humic acid, Fulvic acid, Nitroxin (contains Azotobacter spp. And Azospirillum spp. Bacterias), Biophosphorous (contains Bacillus sp. And Pseudomonas sp. bacterias), Biosulfur (contains Thiobacillus spp. Bacteria), Glomus mosseae, Glomus intraradices, and control. The results showed that organic acids of humic and fulvic increased germination percentage 25 and 20% and germination rate 58 and 33% compared to control, respectively. Germination percentage was 20, 25, and 24% more in biofertilizers of nitroxin, biophosphorous and biosulfur compared to control, respectively and germination rate under application of this fertilizers added 59, 24, and 50% compared to control, respectively. Humic acid, fulvic acid, nitroxin, biophosphorous, biosulfur, Glomus mosseae and Glomus intraradices reduced mean germination time 33, 35, 38, 19, 36, 36, and 16% compared to control, respectively. The highest radicle and plumule length obtained in treatment of humic acid and the highest radicle to plumule length observed in treatment of biosulfur. Fulvic acid increased radicle and plumule dry weight 51 and 38% compared to control, respectively. The highest and the lowest radicle to plumule dry weight obtained in treatments of Glomus intraradices (0.199) and control (0.124), respectively.

Keywords

References

Abdul Jaleel, C., P. Manivannan, B. Sankar, A. Kishorekumar, R. Gopi, R. Somasundaram, and R. Panneerselvam. 2007. Pseudomonas fluorescens enhances biomass yield and ajmalicine production in Catharanthus roseus under water deficit stress. Colloids and Surfaces B: Biointerfaces. 60: 7–11.
Adam, N.R., D.A. Dierig, T.A. Coffelt, and M.J. Wintermeyer. 2007. Cardinal temperatures for germination and early growth of two Lesquerella species. Ind. Crops. Prod. 25: 24-33.
Agha Alikhani, M., A. Iranpour, and H. Naghdi Badi. 2013. Changes in agronomical and phytochemical yield of purple coneflower (Echinaceae purpurea (L.) Moench) under urea and three biofertilizers application. J. Med. Res. 12: 121-136.
Amiri, M.B., R. Ghorbani, M. Jahan, and H.R. Ehyaee. 2012. Evaluate of some germination and emergence characteristics of fennel (Foeniculum vulgare Mill.) produced from seeds under application organic and biological fertilizers. Iranian. J. Field. Crops. Res. 10(4): 649-658. (In Persian)
Amiri, M.B., P. Rezvani Moghaddam, and M. Jahan. 2017. The effects of soil amendments and bio-fertilizers inoculation on morphological characterstics and yield of Echium amoenum. J. Hortic. Sci. 31(1): 25-39. (In Persian)
Amiri, M.B., P. Rezvani Moghaddam, and M. Jahan. 2018. Evaluation of germination characteristics and seedling growth of Iranian Ox-Tongue (Echium amoenum Fisch & Mey.) seed resulting from the rootstock treatmented by biological and chemical fertilizers in different planting dates and methods. Iranian. J. Seed. Sci. Technol. 7(1): 181-199. (In Persian)
Azad, H., B. Fazeli-nasab, and A. Sobhanizadeh. 2017. A study into the effect of jasmonic and humic acids on some germination characteristics of rosselle (Hibiscus sabdariffa) seed under salinity stress. Iranian. J. Seed. Res. 4(1): 1-18. (In Persian)
Baser, S., and B. Mirshekari. 2009. Effect of Nitrajin biofertilizer on germination and primary growth of Brassica napus L., Sesamum indicum L. and Helianthus annus L. Agroecol. J. 5: 91-100. (In Persian)
Baser, S., B. Mirshekari, H. Khoshvaghti, and Sh. Allahyari. 2011. Effect of Nitrajin biofertilizer on germination and primary growth of cumin and caraway. National Conference on New Advances in Agronomy, 16 and 17 September, Ghods city, Iran.
Benabdellah, K., Y. Abbas, M. Abourouh, R. Aroca, and R. Azcon. 2011. Influence of two bacterial isolates from degraded and non-degraded soils and arbuscular mycorrhizae fungi isolated from semi-arid zone on the growth of Trifolium repens under drought conditions: Mechanisms related to bacterial effectiveness. Eur. J. Soil Biol. 47: 303-309.
Chen, J. 2006. The combined use of chemical and organic fertilizers and/or biofertilizer for crop growth and soil fertility. International Workshop on Sustained Management of the Soil-Rhizosphere System for Efficient crop Production and Fertilizer Use. October, 16-20. Thailand.
Copetta, A., G. Lingua, and G. Berta. 2006. Effects of three AM fungi on growth, distribution of glandular hairs, and essential oil peoduction in Ocimum basilicum L. var. Genovese. Mycorrhiza. 16: 485-494.
Darzi, M.T., A. Ghalavand, F. Rejali, and F. Sefidkon. 2007. Effects of biofertilizers application on yield and yield components in fennel (Foeniculum valgare Mill.). Iranian. J. Medicinal. Aromatic Plants. 22(4): 276-292. (In Persian)
Ebrahimi, M., and E. Miri Karbasak. 2016. Investigation effect of humic acid on germination, seedling growth and photosynthesis pigments of medicinal plant isabgol (Plantago ovata Forssk.). Iranian. J. Seed. Sci. Res. 3(3): 35-46. (In Persian)
Fatma, A.G., A.M. Lobna, and N.M. Osman. 2008. Effect of compost and biofertilizers on growth, yield and essential Oil of sweet marjoram (Majorana hortensis) Plant Int. J. Agric. Biol. 10: 381–387.
Ghasemi, K., S. Fallah, F. Raeisi, and M. Heidari. 2014. The effect urea and biological fertilizers on quantitative and qualitative yield of isobgol (Plantago ovata Forsk.) medicinal plant. J. Plant Prod. 20(4): 101-116. (In Persian)
Girish, N., and S. Umesha. 2005. Effect of plant growth promoting rhizobacteria on bacterial canker of tomato. Archives. Phytopathol. Plant Prot. 38: 235-243.
Jack, H., and M. Evans. 2000. Humic acid seed and substrate treatments promote seedling root develop. Hortic. Sci. 35(7): 1231-1233.
Hashemzadeh, F., B. Mirshekari, F.R. Khoe, M. Yarnia, and A. Tarinejad. 2013. Effect of bio and chemical fertilizers on seed yield and its components of dill (Anethum graveolens). J. Medicinal. Plants Res. 7: 111-117.
Jahan, M., A. Koochaki, and M. Nasiri Mahallati. 2007. The effects of arbuscular mycorrhizal fungus and free living nitrogen fixing bacteria on growth, photosynthesis and yield of corn. Iranian. J. Field. Crops. Res. 5(1): 53-68. (In Persian)
Karr, M. 2001. Oxidized lignites and extracts from oxidizwd lignites in agriculture. Soil Sci. 1-23.
Kapoor, R., B. Giri, and K.G. Mukerji. 2002. Glomus macrocarpum: potential bioinoculant to improve essential oil quality and concentration in dill (Anethum gravolens L.) and carum (Trachyspermum ammi (Linn.) Sprague). World. J. Microbiol. Biotechnol. 18: 459-463.
Kapoor, R., B. Giri, and K.G. Mukerji. 2004. Improved growth and essential oil yield and quality in (Foenicudum vulgare mill) on mycorrhizal inoculation supplemented with P-fertilizer. Bioresour. Technol. 93: 307-311.
Kapoor, R., V. Chaudhary, and A.K. Bhatnagar. 2007. Effects of arbuscular mycorrhiza and phosphorus application on artemisinin concentration in Artemisia annua L. Mycrrhiza. 17: 581- 587.
Kızılkaya, R. 2008. Yield response and nitrogen concentrations of spring wheat (Triticum aestivum) inoculated with Azotobacter chroococcum strains. Ecol. Eng. 33:150–156.
Lashkari, A., P. Rezvani Moghaddam, and A. Amin Ghafori. 2014. Estimation of cardinal temperatures of (Echium amoenum) with application of regression models. Iranian. J. Field. Crops. Res. 12(2): 164-169. (In Persian)
Mahfouz, S.A., and M.A. Sharaf-Eldin. 2007. Effect of mineral vs. biofertilizer on growth, yield, and essential oil content of fennel (Foeniculum vulgare Mill.). International. Agrophys. 21: 361-366.
Marulanda, A., R. Porcel, J.M. Barea, and R. Azcon. 2007. Drought tolerance and antioxidant activities in laender plants colonized by native drought-tolerant of drought-sensitive Glomus species. Microbial. Ecol. 54: 543-552.
Massoud, O.N., M.M.I. Afifi, Y.S. El-Akshar, and G.A.M. El-Sayed. 2013. Impact of biofertilizers and humic acid on the growth and yield of wheat grown in reclaimed sandy soil. Res. J. Agric. Biol. 9: 104-113.
Mohammadipour, E., A. Golchin, J. Mohammadi, N. Negahdar, and M. Zarchini. 2012. Effect of humic acid on yield and quality of marigold (Calendula officinalis L.). Ann. Biol. Res. 3: 5095-5098.
Rentato, Y., M.E. Ferreira, M.C. Cruz, and J.C. Barbosa. 2003. Organic matter fraction and soil fertility the influence of liming vermicompost and cattle manure. Bioresour. Technol. 60: 59-63.
Richter. J., M. Stutzer, and I. Schellenberg. 2005. Effects of mycorrhization on the essential oil content and composition of aroma components of marjoram (Marjorana hortensis), thyme (Thymus vulgaris L.) and caraway (Carum carvi L.). 36th International Symposium on Essential Oils, 4-7 September, Budapest, Hungary.
Rillig, M.C., and D.L. Mummey. 2006. Mycorrhizas and soil structure. New. Phytol. 171: 41-53.
Roesti, D., R. Gaur, B.N. Johri, G. Imfeld, S. Sharma, K. Kawaljeet, and M. Aragno. 2006. Plant growth stage, fertilizer management and bio-inoculation of arbuscular mycorrhizal fungi and plant growth promoting rhizobacteria affect the rhizobacterial community structure in rain-fed wheat fields. Soil Biol. Biochem. 38: 1111-1120.
Sahin, F., R. Cakmakci, and F. Kantar. 2004. Sugar beet and barley yields in relation to inoculation with N2-fixing and phosphate solubilizing bacteria. Plant Soil. 265: 123–129.
Salehzadeh, H., M. Izadkhah Shishvan, and M. Chiyasi. 2009. Effect of seed priming on germination and seedling Growth of Wheat (Triticum aestivum L.). J. Biol. Sci. 4: 629-631.
Shahriari, R., E. Gurbanov, A. Gadimov, and D. Hassanpanah. 2008. In vitro effect of potassium humate on terminal drought tolerant bread wheat. Proc. 14th meeting of Int. Humic Substances Soc. 14-20 Sept., 2008. Ville: Moscow. Pays: Russia.
Shaalan, M.N. 2005. Effect of compost and different sources of biofertilizers, on borage plants (Borago officinalis). Egypt. J. Agric. Res. 83: 271-285.
Sidari, M., E. Attina, O. Francioso, V. Tugnoli, and S. Nardi. 2006. Biological activity of humic substances is related to their chemical structure. Soil. Sci. Soc. Am. J. 71: 75-85.
Singh, A.R., and V.G. Maken. 1985. Correlation studies on seed viability and seedling vigour in relation to seed size in sorghum (Sorgum bicolor). Seed. Sci. Technol. 13: 139-142.
Tilak, K.V.B.R., N. Ranganayaki, K.K. Pal, R. De, A.K. Saxena, C. Shekhar Nautiyal, A.K. Tripathi, and B.N. Johri. 2005. Diversity of plant growth and soil health supporting bacteria. Curr. Sci. 89: 136-150.
Vestberg, M., K. Saari, S. Kukkonen, and T. Hurme. 2005. Mycotrophy of crops in rotation and soil amendment with peat influence the abundance and effectiveness of indigenous arbuscular mycorrhizal fungi in field soil. Mycorrhiza. 15: 447-458.
Vessey, J.K. 2003. Plant growth promoting rhizobacteria as biofertilizer. Plant Soil. 255: 571–586.
Wu, S.C., Z.H. Caob, Z.G. Lib, K.C. Cheunga, and M.H. Wong. 2005. Effects of biofertilizers containing N-fixer, P and K solubilizers and AM fungi on maize growth: a greenhouse trial. Geoderma. 125: 155-166.
Yazdani Biuki, R., P. Rezvani Moghaddam, A. Koocheki, M.B. Amiri, J. Fallahi, and R. Deihimfard. 2010. Effects of different nitrogen nutrition of wheat (Sayonese cultivar) on germination characteristics and seedling growth affected by drought stress levels and biofertilizers. Agroecol. 2: 266-276. (In Persian)
Zapata, F., and R.N. Roy. 2004. Use of phosphate rocks for sustainable agriculture. Publication of the FAO Land and Water Development Division, Rome, Italy.
 Zhu, C.X., B.F. Song, and W.H. Xu. 2010. Arbuscular mycorrhizae improves low temperature stress in maize via alterations in host water status and photosynthesis. Plant Soil. 331: 129-137.
Iranian Journal of Seed Science and Technology
Volume 10, Issue 4 - Serial Number 24
Winter of 2022
December 2021
Pages 19-32

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