The effect of humic acid on germination indices and some growth traits of Khuzestani savory (Satureja khuzistanica Jamzad) Andimeshk's ecotype under salinity stress

Document Type : Original Article

Authors

1 Phd student, Lorestan University

2 Plant Production and Genetics Department, Lorestan University

3 Plant Production and Genetics, Lorestan University

4 Plant Production and Gentics, Lorestan University

5 Soil Science , Lorestan University

Abstract

In order to reduce the effects of salinity on germination and growth of Khuzestani savory by humic acid, two experiments were performed. The first experiment was done by priming the seeds with zero concentrations, 20, 40 and 60 mg / l of humic acid (H1, H2, H3 and H4, respectively) and germinating them in zero salinity (distilled water), 50, 25, 75 and 100 mM أNaCl (S1, S2, S3, S4 and S5, respectively) in petri dishes. In the second experiment in the greenhouse, humic acid at zero, 10, 20, 30 and 40 mg / kg soil (H1, H2, H3, H4 and H5, respectively) and salinity stress including zero, 50, 25, 75 and 100 mM sodium chloride (S1, S2, S3, S4 and S5, respectively). The results of the first experiment showed that germination percentage and rate were decreased by increasing in salinity, While, germination rate and percentage were improved by humic acid priming. Salinity also decreased the growth characteristics in pot experiment. While humic acid led to the improvement of these growth traits. With increasing salinity, electrolyte leakage increased, but it was reduced by humic acid. The positive results of using humic acid in reducing the harmful effects of salinity, in addition to laboratory (seed priming) were also proven in pots (soil application). Therefore, in general, according to the positive results of this study on improving the germination and growth indices of Khuzestani savory plant, the use of humic acid can be recommended to reduce the adverse effects of salinity.

Keywords


Abdulbaki, A. A., and J. D. Anderson. 1975. Vigour determination in soybean seed by multiple criteria. Crop Sci. 13:630-633.
Al-Taisan, W. A. 2010. Comparative effects of drought and salt stresses on germination and seedling growth of Pennisetum divisum (Gmel.) Henr. Am. J. Appl. Sci. 7 (5):640-646.
Aydin, A. C., and M. Turan. 2012. Humic acid application alleviates salinity stress of common bean (Phaseolus vulgaris L.) plants decreasing membrane leakage. Afr. J. Agric. 7: 1073-1086.
Bashan, Y., L. E. Bashan, S. R. Prabhu, and J. P. Hernandez. 2014. Advances in plant growth-promoting bacterial inoculant technology: formulations and practical perspectives (1998–2013). Plant and Soil. 378: 1–33.
Bewley, J. D. 1997. Seed germination and dormancy. Plant Cell. 9: 1055-1066.
Bronick, E. J., and R. Lai. 2005. Soil structure and management. A Rev. Geoderma, 124: 3-22.
Camberato, J., and B. Mccarty. 1999. Irrigation water quality: part I. Salinity. South Carolina
Turf. Found. News 6(2): 6-8.
Gulser, F., F. Sonmez, and S. Boysan. 2010. Effects of calcium nitrate and humic acid on pepper seedling growth under saline condition. J. Environ. Biol. 31(5): 873-876.
Eisvand, H. R., and A. Sharafi. 2017. Effects of osmopriming at different temperatures on emergence, seedling growth and essential oil of Savory (Satureja khuzestanica Jamzad) under drought stress. Iranian J. Seed Sci. Technol. 5(2): 23-35.
Jamzad, Z. 1996. Satureja rechingeri (Labiatae) – a new species from Iran. Annuals of Naturhistoriches Museum Wien, 98: 75–77.
Kamkar, A., F. Tooryan, and B.A. Akhondzadeh. 2013. Chemical composition of summer savory (Satureja hortensis L.) essential oil and comparison of antioxidant activity with aqueous and alcoholic extracts. Iranian J. Vet. Res. 68 (2): 183-90. (In Persian, with English Abstract)
Kaya, M.D., G. Okci., M. Atak., Y. Cikili, and O. Kolsarici. 2006. Seed treatment to overcome salt and drought stress during germination in sunflower (Helianthus annuus L.). Eur. J. Agron. 24: 291-295.
Khan, A., R. Gurmani., A. Urman., F. Muhammad., Z. Khan Hussain., M. Ehsan Akhtar, and S. Khan. 2013. Effect of humic acid on the growth, yield, nutrient composition, photosynthetic pigment and total sugar contents of peas (Pisum Sativum L.). J. Chem. Soc. Pakistan. 35(1): 206-211.
Khosropour, M., H. Sodaeizadeh, and M. A. Hakimzadeh. 2016. Investigating the role of humic acid in reducing the effect of salinity stress on satureja species. 4th National Conference on Agriculture and Sustainable Resources. 22 February 2016. Tehran, Iran.
Lezi, A., M. Fambrini., S. Barotti., C. Pugliesi, and P. Vernieri. 1995. Seed germination and seedling growth in wilty mutant of sunflower (Helianthus annuus L.): Effects of abscisic acid and osmotic potential. Environ. Exp. Bot. 35: 427-434.
Najafi, F., R. A. Khanvari-Nejad, and M. Siah Ali. 2010. The effect of salt stress on certain physiological parameters in summer savory (Satureja hortensis) plant. J. Stress Physiol. Biochem. 6: 13-21.
Nakabayashi, R., and K. Saito. 2015. Integrated metabolomics for abioticstress responses in plants. Curr. Opin. Plant Biol. 24: 6-10.
Orabi, S. A., S. R. Salman, and M. A. Shalaby. 2010. Increasing resistance to oxidative damage in cucumber (Cucumis sativus L.) plants by exogenous application of salicylic acid and paclobutrazol. J. Agric. Sci. 6(3): 252-259.
Paksoy, M., O. Turkmen, and A. Dursun. 2010. Effects of potassium and humic acid on emergence, growth and nutrient contents of okra (Abelmoschus esculentus L.) seedling under saline soil conditions. Afr. J. Biotechnol. 9 (33): 5343-5346.
Parihar, P., S. Singh., R. Singh., V. P. Singh, and S. M. Prasad. 2015. Effect of salinity stress on plants and its tolerance strategies: a review. Environ. Sci. Pollut. Res. 22(6): 4056-4075.
Parvin, S., O. R. Lee., G. Sathiyaraj, A. Khoralragchaa, Y. J. Kim, M. J. Miah, and D. C. Yang. 2012. Modulation of polyamine levels in ginseng hairy root cultures subjected to salt stress. Russian J. Plant Physiol. 59(6): 757-765.
Sabzevari, S. and H. Khazaie. 2016. The Effect of foliar application with humic acid on growth, yield and yield components of wheat (Triticum aestivum L.). Agroecol. 1(2): 53-63.‏ (In Persian, with English Abstract)
Samavat, S. and M. Malakuti. 2005. Important use of organic acid (humic and fulvic) for increase quantity and quality agriculture productions. Water Soil Res. Tech. Issue, 463: 1-13. (In Persian, with English Abstract)
Seid Jamali, Z., A. R. Astaraei, and H. Emami. 2015. Effects of humic acid, compost and phosphorus on growth characteristics of basil herb and concentration of micro elements in plant and soil. J. Sci. Technol. Greenhouse Cult. 6 (22): 187-205.
Shamsaie, M., H. Sodaei Zadeh, and M. Tajamolian. 2017. Evaluation of the effects of drought stress of mother plants on some seed germination indices of Satureja hortensis. J. Desert Manage. 4(8): 27-35. (In Persian, with English Abstract)
Sharif, M., K. A. Khattak, and M. S. Sarir. 2002. Effect of different levels of lignitic coal derived humic acid on growth of maize plant. Plant Analysis, 33:3567-3580.
Shiferaw, B., and D.A. Baker. 1996. An evaluation of drought screening techniques for Eragrostis. Trop. Sci. 36: 74-85.
Taize, L. and E. Zaiger. 2002. Plant Physiol. Sinauer Associates, Sunderland, U.K.
Tester, M. and, R. Davenport. 2003. Na2+ tolerant and Na+ transport in higher plants. Ann. Bot. 91: 503-527.
Vojoodi Mehrabani, L., M. B. Hassanpour Aghdam, and R. Valizadeh Kamran. 2017. Study of growth and some physiological traits of sage (Satureja hortensis L.) under salinity stress. J. Ecophysiol. Crop Plants, 41:110-99. (In Persian, with English Abstract)
Yari, M., N. Karimi, H. R. Qasempour, and A. Moradi. 2014. Investigation of the effect of salinity stress on germination and its characteristics in sage medicinal plant Satureja hortensis. The first national conference on medicinal plants, traditional medicine and organic agriculture. Hamedan Shahid Moffteh University. (In Persian, with English Abstract)