Evaluation of the effect of osmo and hydro priming on germination traits of polymorph seeds of Marigold compact petal variety (Calendula officinalis L.)

Document Type : Original Article

Authors

1 Department of Agronomy and plant breeding, Razi University, Kermanshah, Iran.

2 Assistant Professor, Department of Agronomy and plant breeding, Razi University, Kermanshah, Iran.

3 MS. Student, Department of Agronomy and plant breeding, Razi University, Kermanshah, Iran.

4 PhD. Student of, Department of Agronomy and plant breeding, Razi University, Kermanshah, Iran.

Abstract

Seed priming is one of the most effective methods for improving germination and seedling establishment. In order to study the effect of priming on germination of different shapes of Marigold compact petal variety (Calendula officinalis L.), a factorial experiment was conducted in a completely randomized design with three replications in 2014. The germination traits were calculated for different shapes of Marigold seedlings such as germination percentage, germination rate, seedling index, root length and shoot length. The results showed that crescent-shaped seeds exhibited the highest percentage, rate and germination index with 75.51%, 4.48 germination per day and 18.97%, respectively. In addition, the effect of osmo and hydro priming on seeds showed that the highest germination percentage (75.56%) was observed in zero bar of osmo priming and hydro priming of 8 hours. The highest germination rate in the hydro priming treatments was 8 and 16 hours, with values of 3.16 and 3.26 germination per day, respectively. The highest germination index of hydro priming treatments was recorded at 8 and 16 hours with values of 12.22 and 11.75, respectively. Priming action can be effective in reducing the negative effects of various factors during germination and afterwards. Overall, the results of this experiment showed that the use of osmo and hydro priming for the crescent-shaped seeds of Mrigold had the highest germination traits.

Keywords


Agrawal, R. L. 2004. Seed technology Oxford and IBH publishing Co. LTD., New Dehli.
Alizadeh, A. 2001. Soil-Water plant relationship Imam Reza University publishing Co., Mashhad, Iran. (In Persian)
Argerich, C.A., and K. J. Bradford. 1989. The effects of priming and ageing on seed vigour in tomato. J. Exp. Bot. 40:599-607.
Ashraf, M., and M. Foolad 2005. Pre-Sowing Seed Treatment-A Shotgun Approach to Improve Germination, Plant Growth, and Crop Yield Under Saline and Non‐Saline Conditions. Adv.
Agron.88:223-271.
Azzaz,  N., E. Hassan, and F. El-Emarey. 2007. Physiological, anatomical, and biochemical studies on pot marigold (Calendula officinalis L.) plants. Pp. 1727-1738. In Afr. Crop. Sci. Conf. Proc. 8th , 27-31 Oct. 2007. El-Minia, Egypt.
Basch, E., S. Bent, I. Foppa, S. Haskmi, D. Kroll, M. Mele, P. Szapary, C. Ulbricht, M. Vora, and S. Yong. 2006. Marigold (Calendula officinalis L.) An Evidence-Based Systematic Review by the Natural Standard Research Collaboration. J. Herbal Pharmacother. 6:135-159.
Baskin, J. M., and C. C. Baskin. 2004. A classification system for seed dormancy. Seed Sci. Res. 14:1-16.
Bayat, P., M. Ghobadi, M. E. Ghobadi, and G.  R. Mohammadi. 2016. Evaluation the Ability of Standard Germination Test to Predict Emergence and Establishment of Chickpea (Cicer arietinum L.) Seedlings in Field. Iranian J. Seed Sci. Thechnol. 5:27-38. (In Persian)
Bewley, J. D., and M. Black. 1982. Physiology and biochemistry of seeds in relation to germination. Pp 387. In vol 2: Viability, dormancy and environmental control Springer-Verlag, Berlin.
Bradford, K. J. 1986. Manipulation of seed water relations via osmotic priming to improve germination under stress conditions. Hort. Sci. 21(5): 1105-1112.
Bradford, K.J., J. J. Steiner, and S. E. Trawatha. 1990. Seed priming influence on germination and emergence of pepper seed lots. Crop Sci. 30:718-721.
Chojnowski, M., F. Corbineau, and D. Côme. 1997. Physiological and biochemical changes induced in sunflower seeds by osmopriming and subsequent drying, storage and aging. Seed Sci. Res. 7:323-332.
Datta, K.S., and J. Dayal. 1991. Studies on germination and early seedling growth of gram (Cicer arientinum L.) as affected by salinit. Pp 273-276. In K. K. Dhir, I. S. Dua and and K. S. Chark (Eds.), New Trends in Plant Physiology. Today and Tomorrow Printers & Publishers, New Delhi, India.
El-Keblawy, A. 1999. Hyper-variable seed heteromorphy in Egyptian Calendula micronatha Tineo et Guss: effects on Achene dormancy and progeny traits. Union Arab Biol. Cairo. 9:351-370.
Ghassemi-Golezani, K., A. A.Aliloo, M. Valizadeh, and M. Moghaddam. 2008. Effects of hydro and osmo-priming on seed germination and field emergence of lentil (Lens culinaris Medik.). Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 36: 29-33.
Harris, D., A. Joshi, P. Khan, P. Gothkar, and P. Sodhi. 1999. On-farm seed priming in semi-arid agriculture: development and evaluation in maize, rice and chickpea in India using participatory methods. Exp. Agric. 35:15-29.
Harris, D., A. Rashid, Miraj G., M. Arif, and H. Shah. 2007. ‘On-farm’seed priming with zinc sulphate solution—A cost-effective way to increase the maize yields of resource-poor farmers. Field Crops Res. 102:119-127.
Heydecker, W., and P. Coolbear. 1977. Seed treatments for improved performance-survey and attempted prognosis. Seed Sci. Technol. 5:3-425.
Hopper, N., J. Overholt, and J. Martin. 1979. Effect of cultivar, temperature and seed size on the germination and emergence of soya beans (Glycine max (L.) Merr.). Ann Bot 44:301-308.
Imbert, E. 2002. Ecological consequences and ontogeny of seed heteromorphism. Perspect. Plant Ecol. Evol. Syst. 5:13-36.
Kalvatchev, Z., R. Walder, and D. Garzaro. 1997. Anti-HIV activity of extracts from Calendula officinalis flowers. Biomed. Pharmacother. 51:176-180.
Kaya, M. D., G. Okçu, M. Atak, Y. Çıkılı, and Ö. Kolsarıcı. 2006. Seed treatments to overcome salt and drought stress during germination in sunflower (Helianthus annuus L.). Eur. J. Agron. 24:291-295.
Khajeh-Hosseim, M., A. A. Powell, and J. Bingham. 2003. The interaction between salinity stress and seed vigour during germination of soybean seeds. Seed Sci. Technol. 31:715-725.
Khan, M. A., and I. A. Ungar. 2001. Alleviation of salinity stress and the response to temperature in two seed morphs of Halopyrum mucronatum (Poaceae). Aust. J. Bot. 49:777-783.
Liu Y., H. W. Horst, S. P. Groot, and R. J. Bino. 1997. Amounts of nuclear DNA and internal morphology of gibberellin-and abscisic acid-deficient tomato (Lycopersicon esculentum Mill.) seeds during maturation, imbibition and germination. Ann. Bot. 79:161-168.
Marini, R. P. 2003. Approaches to analyzing experiments with factorial arrangements of treatments plus other treatments. Hort. Sci. 38: 117-120.
Matilla, A., M. Gallardo, and M. I. Puga-Hermida. 2005. Structural, physiological and molecular aspects of heterogeneity in seeds: a review. Seed Sci. Res. 15:63-76.
Muley, B., S. Khadabadi, and N. Banarase. 2009. Phytochemical constituents and pharmacological activities of Calendula officinalis Linn (Asteraceae): a review. Trop. J. Pharm. Res. 8: 455-465.
Murungu, F., P. Nyamugafata, C. Chiduza, L. Clark, and W. Whalley. 2003. Effects of seed priming, aggregate size and soil matric potential on emergence of cotton (Gossypium hirsutum L.) and maize (Zea mays L.). Soil Till. Res.74:161-168.
Ruan S., Q. Xue, and K. Tylkowska 2002. Effects of priming on germination and health of rice (Oryza sativa L.) seeds. Seed sci. and technol. 30:451-458.
Samsamshariat, S. 2003. Production and reproduction of medicinal plants Mani press, Esfahan, Iran. pp. 420. (In Persian)
Scott, S. J., R. A. Jones, and W. A. Willams. 1984. Review of data analysis methods for seed germination. Crop Sci 24:1192-1199.
Shivankar, R., D. Deore, and N. Zode. 2003. Effect of pre-sowing seed treatment on establishment and seed yield of sunflower. J. Oilseeds Res. 20:299-300.
Singh, B.G. 1995. Effect of hydration-dehydration seed treatments on vigour and yield of sunflower. Indian J. Plant Physiol. 38:66-68.
Sivritepe, N., H. Sivritepe, and A. Eris. 2003. The effects of NaCl priming on salt tolerance in melon seedlings grown under saline conditions. Scientia Horticulturae. 97:229-237.
Sun, Y. Y., Y. J. Sun, M. T. Wang, X. Y. Li, X. Guo, R. Hu, and J. Ma. 2010. Effects of Seed
Priming on Germination and Seedling Growth Under Water Stress in Rice. Acta Agronomica Sinica. 36:1931-1940.
Tajbakhsh, M. 1996. Seed, identification, control and certification Ahrar press, Tabriz, Iran. (In Persian)
Vidal-Ollivier, E., R. Elias, F. Faure, A. Babadjamian, F. Crespin, G. Balansard, and G. Boudon. 1989. Flavonol glycosides from Calendula officinalis flowers. Planta Medica. 55:73-74.
Wang, L., Z. Huang, C. C. Baskin, J. M. Baskin, and M. Dong. 2008. Germination of Dimorphic Seeds of the Desert Annual Halophyte Suaeda aralocaspica (Chenopodiaceae), a C4 Plant without Kranz Anatomy. Ann. Bot. 102:757-769.
Wang, Y., J. Zhang, H. Liu, and X. Hu. 2004. Physiological and ecological responses of alfalfa and milkvetch seed to PEG priming. Acta. Ecol. Sin. 24:402-408.
Wei, Y., M. Dong, and Z. Y. Huang. 2007. Seed polymorphism, dormancy and germination of Salsola affinis (Chenopodiaceae), a dominant desert annual inhabiting the Junggar Basin of Xinjiang, China. Aust. J. Bot. 55:464-470.
Zhang, J. 1995. Differences in phenotypic plasticity between plants from dimorphic seeds of Cakile edentula. Oecologia. 102:353-360.