Investigating different levels of salinity and drought stress on the germination and growth of Pennisetum divisum

Document Type : Original Article

Authors

1 University of Tehran College of Agriculture & Natural Resources Faculty of Agricultural Engineering and Technology Department of Horticulture Sciences and Landscape

2 Department of Forestry and Forest Economic, Faculty of Natural Resources, Tehran University

Abstract

Research on the effect of salinity and drought stress on germination and growth pattern among pasture grass populations is very important to determine the potential of suitable species for restoration and protection as well as fodder production. For this purpose, a research was carried out to evaluate the salinity and drought stress of the Pennisetum divisum plant in the germination and seedling growth stage of 2019 in the seed laboratory of the Faculty of Agriculture of Tehran-Karaj University in the form of a completely randomized design and in 3 replications. The treatments of this experiment included 4 levels of salinity (100-150-200 and 300 mM sodium chloride), 4 levels of dryness (2-, 4-, 6- and 8-bar polyethylene glycol) and distilled water (control). The evaluated traits included germination percentage, germination speed, stem length and root length. The results showed that the germination percentage, germination speed, root length and shoot length increased with the increase of salinity stress up to 200 mM and then decreased so that practically no seeds germinated. In drought stress, the studied traits increased up to -6 times and then started to decrease. The results showed that the Pennisetum divisum plant is relatively resistant to salt and drought stress.

Keywords


Akhter, R., and M. Arshad. 2006. Arid rangelands in the Cholistan Desert (Pakistan). Sécheresse. 17:210–217.
Al-Awadhi, J, M., R. F. Misak, and S.S. Omar. 2003. Causes and consequences of desertification in Kuwait: a case study of land degradation. Bull. Eng. Geol. Environ. 62:107–115.
Allah, C.R. 1999. Grown tomato under saline conditions. In: Allah C. R., ed. Plant Salinity. Research, New Chellenges. 23:201-1203.
Amiri, M.B., P. Rezvani Moghadam, H.R. Ehyaii, J. Fallahi, and M. Aghhavani Shajari. 2010. The effects of drought and salinity stresses on germination indices and seedling growth of two medicinal plants as Cynara scolymus L. and Echinaceae purpurea L. Environmental Stresses in Crop Sciences. 3:165-176.
Anju, U.L., S.R. Doddagoudar, S.K. Pattanashetti, G. Basave, and K. Vijaykumar. 2019. Influence of seed priming on seed germination, seedling growth, peroxidase activity, proline and total soluble sugar content of pearl millet (Pennisetum glaucum L.) under salinity stress. Int. J. Chem. Stud. 7:508-514.
Anwari, M., H. Mahdikhani, A. Shahriari, and G. Nouri. 2009. The effect of salinity stress on the germination of seven pasture species. Scientific-Research Quarterly Journal of Pasture and Desert Research in Iran. 16: 262-273.
Asghari, M. 1992. Effect of ethylene on osmotic adjustment and axial tissue and cotyledon development of sunflower seed in water stress condition. Agric. Sci. Technol. J. 7:137-145.
Ayaz, F.A., A. Kadioglu, and R. Turgut. 2000. Water stress effects on the content of low molecular weight carbohydrates and phenolic acids in Cienanthe setosa. Can. J. Plant Sci. 80:373-378.
Azarnivand, H., and M. Javadi. 2005. Investigation of the effect of drought stress on the germination of two types of Agropyron pasture". Biyaban Magazine. 2:8-193.
Bajji, M., J.M. Kinet, and S. Lutts. 2002. Osmotic and ionic effects of NaCl on germination, early seedling growth and ion content of Atriplex halimus (Chenopodiaceae). Can. J. Bot. 80:297-304.
Bassirirad, H., and M.M. Caldwell. 1992. Root growth, osmotic adjustment and NO3 - uptake during and after a period of drought in Artemisia tridentata. Aust. J. Plant Physiol. 19:493-500.
Benidire, K., Z. Daoui, A. Fatemi, W. Achouak, L. Bouarab, and  K. Oufdou. 2015. Effect of salt stress on the germination and development of seedlings of Vicia faba L. J. Mater. About. Sci. 6:840-851.
Bhatt, A., P.C. Phondani, and M.F. Pompelli. 2016. Seed maturation time influences the germination requirements of perennial grasses in desert climate of Arabian Gulf. Saudi J. Biol. Sci. 25:1562–1567.
Bhatt, A., N.R. Bhat, A. AL-nasser, M.M. Caron, and A. Santo. 2020. Inter-population variabilities in seed mass and germination of Panicum turgidum and Pennisetum divisum in the desert of Kuwait. J. Arid Land. 12:144–153.
Dadkhah, A. 2010. Salinity effect on germination and seedling growth of four medicinal plants. Iranian J.  Med. Aromat. Plants. 26:358-369.
Ebrahimi, O., M. Mohammad, H. Esmaeili, H. Sabouri, and A. Tahmasebi. 2012. Effects of salinity and drought stresses on germination of two rangeland plants of Agropyron elongatum and Agropyron desertorum. Desert Ecosyst. Eng. J.1:31-38.
El-hamamsy, S.M.A., and H.A.Z. El-khamissi. 2018. Morphological and biochemical evaluation of some Egyptian wheat genotypes under salini. Biol Chem. Environ. Sci. 13:45-62.
El-Keblawy, A. 2013. Impacts of dormancy regulating chemicals on innate and salinity-induced dormancy of four forage grasses native to Arabian deserts. Grass Forage Sci. 68:288–296.
El Mekkaoui, M. 1987. Contribution to the study of salt tolerance in durum wheat (Triticum durum Desf.) And barley (Hordeum vulgare L.). Diploma of DAA-Agricultural engineer, Montpellier. 1:98-105.
El Mekkaoui, M. 1990. Study of the mechanisms of tolerance to salinity in durum wheat (T. durum Desf.) And barley (H. vulgare L.): research of early selection tests. Doctoral thesis in Agronomic Sciences, USTLMontpellier. 1:191-199.
Farkhah, A., H. Heydari Sharifabad, M. Gurbanli, and H. Shakerbazarno. 2003. The effect of salinity on the germination of three species of salsola, Aeluropus lagopoides and Alhagi persarum, dendroides. Sci. Res. Q. Genet. Res. Breed. Pastur For. Plants Iran. 11:1-13. (In Persian, with English Abstract)
Pourmohammadi, S., and M. Taghi Dasturani. 2015. Investigating the features of the flow and sediment regime in arid and semi-arid areas, a case study: Lorestan province. Geogr. Dev. 4:195-210. (In Persian, with English Abstract)
Gholami, P., J. Ghorbani, and S.h. Ghaderi. 2012. The investigation of seed germination and early growth of Stipa arabica Trin. & Rupr. Under drought and salinity. Iranian J. Plant Ecosyst. 8:17-27. (In Persian, with English Abstract)
Gholami, P., J. Ghorbani, S.h.G. haderi, F. Salarian, and A. Karimzadeh. 2010. Assessment of germination indices for Vicia monantha under salinity and drought stresses. Iranian J. Range. Res. 4:1-11. (In Persian, with English Abstract)
Ghaderi, S.h., J. Ghorbani, P. Gholami, A. Karimzadeh, and F. Salarian. 2011. Effect of drought and salinity stress on germination indices of Vicia villosa L. J. Agroecol. 3:121-130.
Gulzar, S., and M. Ajmal Khan. 2001. Seed germination of a halophytic grass Aeluropus lagopoides. Ann. Bot. 87:319-324.
Hadi, M.R., R. Taheri, and M.A. Sharif. 2007. Study effects of salinity on the seed germination of Seidlitzia rosmarinus. Pajouhesh and Sazandegi. 76:151-157. (In Persian)
Hassan, F., and M. Hassan. 2018. The use of seed vigour tests for predicting field emergence in Acacia senegal and Acacia mellifera. Int. J. Appl. Sci. 1:93-99.
Hosseini, H., and P. Rezvani Moghadam. 2006. Effect of water and salinity stress in seed germination on Isabgol (Plantago ovata). (In Persian, with English Abstract). Iranian J. Field Crops Res.4:15-22. (In Persian, with English Abstract)
Kafi, M., A. Nezami, H. Hosaini, and A. Masomi. 2005. Physiological effects of drought stress by polyethylene glycol on germination of lentil (Lens culinaris Medik.) genotypes. Agron. Res. Iran. 3:69-80. (In Persian, with English Abstract)
Kajary, A., and A. Zinli. 1999. The effect of salinity and drought on germination and seedling growth of two cotton cultivars. (In Persian, with English Abstract). Seedlings and Seeds. 18:500-5003.
Karimi, Q., H. Heidari Sharifabad, and M.H. Extract. 2004. Effects of Salinity Stress on Germination, Seedling Establishment and Peroline Content in Atriplex Verrucifera Species. Genet. Res. Modif. For. Range. Plants. 12:432-419. (In Persian, with English Abstract)
Kaya, M., G. Okcu, M. Atak, Y. Cikili, and O. Kolsarci. 2006. Seed treatment to overcome salt and drought stress during germination in sunflower (Helianthus annuus L.). Eur. Sci. J. 24:291-295.
Keshavarz Afshar, R., M. Kikhavad, M. Chaichi, and M. Ansari. 2012. The effect of different levels of salinity and drought on germination characteristics and seedling turnip forage. Iranian J. Crop Sci.4:661-671. (In Persian, with English Abstract)
Marchner, H. 1995. Mineral Nutrition of Higher Plants. Academic Press, Amsterdam, Netherlands.
Mallek-Maalej, E., F. Boulasnem, and M. Ben Salem. 2004. Effect of salinity on the germination of cereal seeds cultivated in Tunisia. Agric. Notebooks. 12:153-156.
Majnon Hoseini, N., R. Tavakol Afshari, and S. Ehsanfar. 2002. Effects of drought stress induced by PEG in seed germination indices of wheat. Proc. 8thIranian Natl. Congr. Crop Sci, Iran. (In Persian, with English Abstract)
Mganga, K.Z., N.K.R. Musimba, and D.M. Nyariki. 2015. The choice of grass species to combat desertification in semi-arid Kenyan rangelands is greatly influenced by their forage value for livestock. Grass Forage Sci. 70:161–167.
Michel, B.E., and M.R. Kaufmann. 1973. The osmotic potential of polyethylene glycol 6000. Plant Physiol. 51:914-916.
Mittova, V.M., M. Guy, and M. Volokita. 2004. Salinity upregulates the antioxidative system in root mitochondria and peroxisomes of the wild salt-tolerant tomato species Lycopersicon pennellii. J. Exp. Bot. 55:1105-1113.
Mosleh Arani, A., G. Bakhshi Khaniki, N. Nemati, and M. Soltani. 2002. Investigation of the effect of salinity stress on germination and seed vigor in three species of Salsola (Salsola arbuscula, Salsola yazdiana, Salsola abarghuensis). Two Q. J. Genet. Res. Range. For. Plant Breed. Iran. 18:267-279. (In Persian, with English Abstract)
Mukhopadhyay, R., C.T. Hash, A.G.B. Raj, and P.B.K. Kishor. 2005. Assessment of opportunities to map pearl millet tolerance to salinity during germination and early seedling growth. Int. Sorghum Millets Newsl. 46:117-119.
Osman, A.E., M. Makawi, and R. Ahmed. 2008. Potential of the indigenous desert grasses of the Arabian Peninsula for forage production in a water-scarce region. Grass Forage Sci. 63:495–503.
Peacock, J.M.F., M.E. Erguson, and G.A. Alhadrami. 2003. Conservation through utilization: a case study of the indigenous forage grasses of the Arabian Peninsula. J. Arid Environ. 54:15–28.
Pirasteh-Anosheh, H., G. Ranjbar, Y. Emam, and M. Ashraf. 2014. Salicylic acid–induced recovery ability in saltstressed Hordeum vulgare plants. Turk. J. Bot. 38:112-121.
Saadallah, K., J.J. Drevon, and C. Abdelly. 2001. Nodulation and nodular growth in beans (Phaseolus vulgaris) under saline stress. Agronomy. 21:627–634.
Shalata, A., V. Mittova, M. Volokita, M. Guy, and M. Tal. 2001. Response of the cultivated tomato and it's wild salt-tolerant relative Lycopersicon pennellii to salt-dependent oxidative stress: The root antioxidative system. Physiol. Plant. 112:487-494.
Sharma, A.D., M. Thakur, M. Rana, and K. Singh. 2004. Effect of plant growth hormones and abiotic stresses on germination, growth and phosphates activities in Sorghum bicolor L. Moench seeds. Afr. J. Biotechnol. 3:308-312.
Soltani, A., M. Gholipoor, and E. Zeinali. 2006. Seed reserve utilization and seedling growth of wheat as affected by drought and salinity. (In Persian, with English Abstract). Environ. Exp. Bot. 55:195-200.
Souleymane, S., C. Brahima, K. Tchoa, T. Seydou, K. Adjata, K. Daouda, and Z. Michel. 2020. Salinity Stress Effect on the Germination of Three Cereals: Maize (Zea mays), Millet (Pennisetum glaucum) and Rice (Oriza sativa). J. Plant Soil Sci. 32:69-78.
Tamartash, R., F. Shokrian, and M. Kargar. 2010. Effects of salinity and drought stress on Trifolium alexanderium L. seed germination properties. Rangeland. 4:288-297.
Taisan, W. 2010. Comparative Effects of Drought and Salt Stress on Germination and Seedling Growth of Pennisetum divisum (Gmel.) Henr. Am. J. Appl.Sci. 7: 640-646.
Wang, Z., A.C. Bovik, H.RSheikh, and E.P. Simoncelli. 2004. Study on embryology of Haloxylon ammodendron I. Preliminary observation on microsporogenesis and formation of male gametophytes and apomixis in megasporogenesis. J. Desert Res. 24:768-772.
Weisz, P.R., R.F. Denison, and T.R. Sinclair. 1985. Response to drought stress of nitrogen fixation (acetylene reduction) rates by field-grown soybeans. Plant Physiol. 78:525-530.
Werner, J.E., and R.R. Finkelstein. 1995. Arabidopsis mutants with reduced response to NaCl and osmotic stress. Acta Physiol. Plant. 93:659-666.
Yakubu, H., A.L. Ngala, and Y. Dugje. 2010. Screening of Millet (Pennisetum glaucum L.) varieties for salt tolerance in semi-arid soil of northern Nigeria. World J. Agric. Sci. 6:374-380.
Zahtabyan, G., and M. Javadi. 2003. Investigating the effect of drought stress on the germination of three pasture species of Salsola genus Salsola richteri - Salsola rigida - Salsola dendroides. Desert. 8:20-32.
Zahtabian, G., H. Azarnivand, M. Javadi, and E. Shahriari. 2005. Investigation of the effect of salinity stress on the germination of two types of pasture from the genus Agropyron". Biaban Mag. 10: 301-310. (In Persian)