The effect of cold plasma on seed germination and seedling growth characteristics of soybean commercial cultivars

Document Type : Original Article

Authors

1 Department of Physics, Faculty of Converging Sciences and Technologies, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Assistant Professor of Physics, Department of Physics, Faculty of Converging Sciences and Technologies, Science and Research Branch, Islamic Azad University, Tehran

3 Agriculture Research, Education and Extension Organization-Seed and Plant Certification and Registration Institute

4 Department of Biology, Faculty of Converging Sciences and Technologies, Science and Research Branch, Islamic Azad University, Tehran

5 Research Assistant Professor of Agricultural Research, Education and Extension Organization (AREEO), Seed and Plant Certification and Registration Institute (SPCRI), Karaj

Abstract

In order to investigate the effect of cold plasma on seed germination characteristics and seedling growth of soybean commercial cultivars, an experiment in the form of a nest-factorial design with four replications was performed at the Plasma Physics Research Center of Islamic Azad University, Tehran Research Sciences Branch and Seed and Plant Certification and Registration Institute (SPCRI) in 2021. Seeds were exposed to cold plasma for 30, 60, 180, 300 and 420 seconds. In general, germination percentage from 0 to 66.97%, germination rate from 0 to 32.17%, germination index from 3.61 to 60.09%, mean germination time from 4.62 to 66.66%, root length 2.87 to 56.13%, seedling length 2.70 to 78.13%, normal seedling number 0 to 30.26% and seedling dry weight 1.36 to 36.63% increased compared to control treatment. The results of our study showed that 60 seconds treatment had the most positive effect on seed germination characteristics and seedling growth of soybean.

Keywords


Dhayal, M., S.Y. Lee, and S.U. Park. 2006. Using low-pressure plasma for Carthamus tinctorium L. seed surface modification. Vacuum. 80(5): 499–506.
Domonkos, M., Tichá, P., Trejbal, J. and P. Demo. 2021. Applications of Cold Atmospheric Pressure Plasma Technology in Medicine, Agric. Food Ind. Appl. Sci. 11(11). 4809. Doi:10.3390/app11114809.
Don, R., and S. Ducournau. 2018. Handbook on seedling evaluation (3rd. Ed.). International Seed Testing Association (ISTA), Zürichstr. Bassersdorf, Switzerland.
Fereydoni, M., and H. Alizaheh. 2022. Microscopic investigation of cold plasma effect on chickpea seed germination. J. Agric. Machinery. 12(2): 231-240. (In Persian, with English Abstract)
Gómez-Ramírez, A., C. López-Santos, M. Cantos, J.L. García, R. Molina, J. Cotrino, J. Espinós, and A.R. González-Elipe. 2017. Surface chemistry and germination improvement of Quinoa seeds subjected to plasma activation. Sci. Rep. 7(1): 1-12.
Goudarzi, SH., H. Ghafoorifard, S.A. Ghasemi, and A. Mazandarani. 2021. The effect of atmospheric cold plasma on the rates of germination and root length and shoot length of sesame seed. 27th Iran Nuclear Conf. Mar. 1-7. (In Persian, with English Abstract)
Guo, Q., Y. Meng, G. Qu, T. Wang, F. Yang, D. Liang, and S. Hu. 2018. Improvement of wheat seed vitality by dielectric barrier discharge plasma treatment. Bioelectromagnetics. 39(2): 120-131.
Hosseini, S.I., S. Mohsenimehr, J. Hadian, M. Ghorbanpour, and B. Shokri. 2018. Physico-chemical induced modification of seed germination and early development in artichoke (Cynara scolymus L.) using low energy plasma technology. Phys. Plasmas. 25(1): 013525.
International Seed Testing Association (ISTA). 2020. International rules for seed testing. Seed Science and Technology. International Seed Testing Association (ISTA), Zürichstr. Bassersdorf, Switzerland.
Ji, S.H., K.H. Choi, A. Pengkit, J.S. Im, J.S. Kim, Y.H. Kim, Y. Park, E.J. Hong, S. kyung Jung, and E.H. Choi. 2016. Effects of high voltage nanosecond pulsed plasma and micro DBD plasma on seed germination, growth development and physiological activities in spinach. Arch. Biochem. Biophys. 605: 117-128.
Jiang, J.F., X. He, L. Li, J.G. Li, H.L. Shao, Q.L. Xu, H.R. Ye, and Y.H. Dong. 2014. Effect of cold plasma treatment on seed germination and growth of wheat. Plasma Sci. Technol. 16: 54–58.
Li, Y., T. Wang, Y. Meng, G. Qu, Q. Sun, D. Liang, and S. Hu. 2017. Air atmospheric dielectric barrier discharge plasma induced germination and growth enhancement of wheat seed. Plasma Chem. Plasma Process. 37: 1621–1634.
Ling, L., J. Jiafeng, L. Jiangang, S. Minchong, H. Xin, S. Hanliang, and D. Yuanhua. 2014. Effects of cold plasma treatment on seed germination and seedling growth of soybean. Sci. Rep. 4(1): 1-7.‏
Meng, Y., G. Qu, T. Wang, Q. Sun, D. Liang, and S. Hu. 2017. Enhancement of germination and seedling growth of wheat seed using dielectric barrier discharge plasma with various gas sources. Plasma Chem. Plasma Process. 37(4): 1105-1119.
Misra, N.N., O. Schlüter, and P.J. Cullen. 2016. Cold plasma in food and agriculture: fundamentals and applications. Academic Press, U.S.
Noormohammadi, Z., M. Mohammadzadeh-Shahir, D. Fahmi, S.M. Atyabi, and F. Farahani. 2019. Induced genetic and morphological changes in Catharanthus roseus L. by cold atmospheric plasma. Nova Biologica Reperta. 6 (3): 302-310.
Pérez-Pizá, M.C., E. Cejas, C. Zilli, L. Prevosto, B. Mancinelli, D. Santa-Cruz, G. Yannarelli, and K. Balestrasse. 2020. Enhancement of soybean nodulation by seed treatment with non–thermal plasmas.  Sci. Rep. 10(1): 1-12.
Pizá, M.C.P., L. Prevosto, C. Zilli, E. Cejas, H. Kelly, and K. Balestrasse. 2018. Effects of non–thermal plasmas on seed-borne Diaporthe/Phomopsis complex and germination parameters of soybean seeds. Innovative Food Sci. Emerging Technol. 49: 82-91.‏
Ranal, M., and D.G. De Santana. 2006. How and why to measure the germination process? Revista Brasilian Botanique. 29(1): 1-11.
Selcuk, M., L. Oksuz, and P. Basaran. 2008. Decontamination of grains and legumes infected with Aspergillus spp. and Penicillum spp. by cold plasma treatment. Bioresour. Technol. 99: 5104–5109.
Šerá, B., V. Scholtz, J. Jirešová, J. Khun, J. Julák, and M. Šerý. 2021. Effects of non-thermal plasma treatment on seed germination and early growth of leguminous plants -A review. Plants. 10(8): 1616.‏
Švubová, R., L. Slováková, L. Holubová, D. Rovňanová, E. Gálová, and J. Tomeková. 2021. Evaluation of the impact of cold atmospheric pressure plasma on soybean seed germination. Plants. 10(1): 177.
Tong, J., R. He, X. Zhang, R. Zhan, W. Chen, and S. Yang. 2014. Effects of atmospheric pressure air plasma pretreatment on the seed germination and early growth of Andrographis paniculata. Plasma Sci. Technol. 16(3): 260.‏
Wu, Z.H., L.H. Chi, S.F. Bian, and K.Z. Xu. 2007. Effects of plasma treatment on maize seeding resistance. J. Maize Sci. 15: 111–113.
Yin, M.Q., M.J. Huang, B.Z. Ma, and T.C. Ma. 2005. Stimulating effects of seed treatment by magnetized plasma on tomato growth and yield. Plasma Sci. Technol. 7: 3143–3147.
Zhou, Z.W., Y.F. Huang, S.Z. Yang, and W. Chen. 2011. Introduction of a new atmospheric pressure plasma device and application on tomato seeds. Agric. Sci. 2: 23–27.