Improvement of alpha- and beta-amylase enzymes activities and germination indices in safflower variety (Carthamus tinctorius L.) seeds by foliar application of silicon and nickel in saline soil

Document Type : Original Article

Authors

1 Ph.D Student Of Agronomy, Fasa Islamic azad University

2 Islamic azad univecity, Fasa branch, faculty of agriculture

3 Assistant Proffessor, Department of Agricultur, Islamic azad University Fasa branch

Abstract

Salinity stress reduces seed quality, but suitable concentrations of silicon and nickel can improve germination indices in the produced seeds. This study was conducted in a factorial arrangement based on randomized complete block design (RCBD) in 2018 in Haji Abad city, Hormozgan, Iran. The experimental treatments consisted of four safflower varieties as the first factor, including Soffeh, Local Isfahan, Padideh, and Golmehr; and seven spraying levels, including nickel (100, 200 and 300 mg L-1) and silicon (100, 150, and 200 mg L-1) as the second factor. The results demonstrated that the foliar application of 100 mg/l Nickle and 200 mg/l silicon increased potassium by 10% and 18%, respectively. The foliar application of 200 mg/l silicon increased the activity of alpha and beta amylase enzymes by 17% and 12%, respectively. In addition, Golmehr and Safe varieties obtained from a mother plant recorded higher germination and rate percentage, as well as seed vigor, while Padideh variety showed the lowest values of the same traits. The foliar application of 200 mg/l silicon, compared to the control, increased the seed vigor as well as Soffeh and Golmehr cultivars by 23% and 25%, respectively; however, the Nickle concentration of 300 mg/l resulted in a decrease of 24% and 19% in these cultivars.On the other, this trait decreased with increasing nickel concentration significantly. Generally, foliar application of silicon at concentrations of 150 and 200 mg L-1 was found to improve seed quality of different safflower varieties under salinity stress.

Keywords


Afzal, I., R. Shabir, and S. Rauf. 2019. Seed production technologies of some major field crops. Pp 655-678. In M. Hasanuzzaman(eds.)Agronomic Crops. Springer, Singapore.
Ain, Q., J. Akhtar, M. Amjad, M. Haq, and Z. Saqib. 2016. Effect of enhanced nickel levels on wheat plant growth and physiology under salt stress. Commun. Soil Sci. Plant Anal. 47: 2538–2546.
Al-Garni, S.M.S., M.M.A. Khan, and A. Bahieldin. 2019. Plant growth-promoting bacteria and silicon fertilizer enhance plant growth and salinity tolerance in Coriandrum sativum. J. Plant Interact. 14: 386-396.
Ali, M., S. Afzal, A. Parveen, M. Kamran, M.R. Javed, G.H. Abbasi, Z. Malik, M. Riaz, S. Ahmad, and M.S. Chattha. 2021. Silicon mediated improvement in the growth and ion homeostasis by decreasing Na+ uptake in maize (Zea mays L.) cultivars exposed to salinity stress. Plant Physiol. Biochem. 158: 208-218.
Alzahrani, Y., A. Kuşvuran, H.F. Alharby, S. Kuşvuran, and M.M. Rady. 2018. The defensive role of silicon in wheat against stress conditions induced by drought, salinity or cadmium. Ecotoxicol. Environ. Saf. 154: 187–196.
Ameen, N., M. Amjad, B. Murtaza, G. Abbas, M. Shahid, M. Imran, M. A. Naeem, and N. K. Niazi. 2019. Biogeochemical behavior of nickel under different abiotic stresses: toxicity and detoxification mechanisms in plants. Environ. Sci. Pollut. Res. 26: 10496–10514.
Anonymus. 2010. International rules for seed testing. International Seed Testing Association (ISTA). Seed Science and Technology. Zurich, Switzerland.
Attarzadeh, M., H.R. Balouchi, M. Dehnavi, A. Salehi, and, M. Rajaie2019. Response of germination and electrical conductivity of seeds produced by Echinacea purpurea's mother plants under the influence of biological fertilizers and drought stress. Iranian J. Seed Sci. Technol. 8: 185-200. (In Persian, with English Abstract)
Barcelo, J., and C. Poschenrieder. 2004. Structural and ultrastructural changes in heavy metal exposed plants. Pp 223–248. In M.N.V. Prasad (ed.) Heavy metal stress in plants, 3rd ed. Springer, Berlin, Germany.
Bassil, E.S., and S. R. Kaffka. 2002. Response of safflower (Carthamus tinctorius L.) to saline soils and irrigation: I. Consumptive water use. Agric. Water. Manage. 54: 67-80.
Bernfeld, P. 1955. Amylase α and β. Methods in Enzymology. 1: 149-158.
Bijanzadeh, E., S. M. Moosavi, and F. Bahadori. 2022. Quantifying water stress of safflower (Carthamus tinctorius L.) cultivars by crop water stress index under different irrigation regimes', Heliyon. 8: e09010.
Chahardoli, A., N. Karimi, X. Ma, and F. Qalekhani. 2020. Effects of engineered aluminum and nickel oxide nanoparticles on the growth and antioxidant defense systems of Nigella arvensis L. Sci. Rep. 10: 1-11.
Copeland, L.O., and M.B. McDonald, 2001. Seed vigor and vigor tests. Pp 121-144. In L.O. Copeland and M.B. McDonald (eds.). Principles of Seed Science and Technology. 4th ed. Kluwer Academic Publishing Group, Norwell, Massachusetts, U.S.
Czajka, K.M., P. Michael, and K. Nkongolo. 2019. Differential effects of nickel dosages on in vitro and in vivo seed germination and expression of a high affinity nickel-transport family protein (AT2G16800) in trembling aspen (Populus tremuloides). Ecotoxicology. 28: 92-102.
Dehshiri, A., and A. Modares Sanavy. 2015. Assessment of seed germination and seedling early growth characteristics of canola (Brassica napus L.) produced from original plants under salinity stress. Iranian J. Seed Sci. Technol. 4: 97-106. (In Persian, with English Abstract)
Dornbos, D. L. 2020. Production environment and seed quality. Pp 119-152. In R.E. Gough (ed.) Seed quality. CRC Press, Boca Raton, U.S.
Emongor, V., O. Oagile, D. Phuduhudu, and P. Oarabile. 2017. Safflower production. Botsawana University of Agriculture and Natural Resources. Impression House Publishers. Industrial West, Gaborone, Botswana.
Garg, N. and P. Bhandari. 2016. Silicon nutrition and mycorrhizal inoculations improve growth, nutrient status. K+ /Na+ ratio and yield of Cicer arietinum L. genotypes under salinity stress. J. Plant Growth Regul. 78: 371–387.
Gimbi, D. M., and N. Kitabatake. 2002. Changes in alpha-and beta-amylase activities during seed germination of African finger millet. Int. J. Food Sci. Nutr. 53(6): 481-488.
Gou, T., X. Chen, R. Han, J. Liu, Y. Zhu, and H. Gong. 2020. Silicon can improve seed germination and ameliorate oxidative damage of bud seedlings in cucumber under salt stress. Acta Physiol. Plant. 42: 1-11.
Gupta, A. 2022. Mainstreaming of underutilized oilseed safflower crop through biotechnological approaches for improving economic and environmental sustainability. Pp 397-418. A. Sudipti, K. Ashwani, O. Shinjiro, Y. Yuan –Yeu (eds.) Biotechnological Innovations for Environmental Bioremediation. Springer, Singapore.
Haghighi, M., Z. Afifipour, and M. Mozafarian. 2012. The alleviation effect of silicon on seed germination and seedling growth of tomato under salinity stress. Veg. Crops Res. Bull. 76: 119-126.
Houshmand, S., A. Arzani, S. A. Maibody, and M. Feizi. 2005. Evaluation of salt-tolerant genotypes of durum wheat derived from in vitro and field experiments. Field Crop Res. 91: 345-354. 
Hu, Y., and U., Schmidhalter, 2005. Drought and salinity: a comparison of their effects on mineral nutrition of plants. J. Plant Nutr. Soil Sci. 168: 541-549.
ISTA. 2003. Handbook for seedling evaluation (3rd.ed). International Seed Testing Association. Zurich, Switzerland.
Johnson, R., and J.T. Puthur. 2021. Seed priming as a cost effective technique for developing plants with cross tolerance to salinity stress. Plant Physiol. Biochem. 162: 247-257.
Kanellou, E., M. Papafotiou, G. Economou, and N. Ntoulas. 2016. Testing soil solarization as an alternative method for weed, suppression at archaeological sites. Presented in VIth International Conference on Landscape and Urban Horticulture. Athens, Greece on 20-25th June 2016. ISHS Acta Hortic.1189: 69-72.
Kheybari, M., A.H. Shirani Rad, S. Seyfzadeh, I. Hadidi, and H.R. Zakerin. 2019. Investigation of sowing date of mother plant effect on germination indices of autumn rapeseed cultivars and lines. Iranian J. Seed Sci. Technol. 7: 237-246. (In Persian, with English Abstract)
Kordrostami, M., and B. Rabiei. 2019. Salinity stress tolerance in plants: physiological, molecular, and biotechnological approaches. Pp 101-127. In M. Hasanuzzaman, Kh. Rehman Hakeem, K. Nahar, H.F. Alharby. Plant abiotic stress tolerance. Springer, Cham.
Kumar, O., S. K. Singh, A. M. Latare, and S. N. Yadav. 2018. Foliar fertilization of nickel affects growth, yield component and micronutrient status of barley (Hordeum vulgare L.) grown on low nickel soil, Arch. Agron. Soil Sci. 64: 1407-1418.
Lowry, C. J., and R.G. Smith. 2018. Weed Control Through Crop Plant Manipulations. Pp 73-96. In Kh. Jabran, and B. Chauhan (eds.) Non-Chemical Weed Control. Academic Press, Amsterdam.
Maguire, J. D. 1962. Speed of germination – aid in selection and evaluation for seedling emergence and vigour. Crop Sci. 2: 176-177.
McGuire, S., and L. Sperling. 2013. Making seed systems more resilient to stress. Global Environ. Change. 23: 644-653.
Miransari, M., and D. Smith. 2014. Plant hormones and seed germination. Environ. Exp. Bot. 99: 110-121.
Morales-Cedillo, F., A. Gonzalez-Solis, L. Gutiérrez-Angoa, D. L. Cano-Ramírez, and M. Gavilanes-Ruiz. 2015. Plant lipid environment and membrane enzymes: the case of the plasma membrane H+-ATPase. Plant Cell Rep. 34: 617-629.
Naveed, M., H. Sajid, A. Mustafa, B. Niamat, Z. Ahmad, M. Yaseen, M. Kamran, M. Rafique, S. Ahmar, and J. Chen. 2020. Alleviation of salinity-induced oxidative stress, improvement in growth, physiology and mineral nutrition of canola (Brassica  napus L.) through calcium-fortified composted animal manure. Sustainability. 12 (3): 846. https://doi.org/10.3390/su12030846
Nguyen, C.D., J. Chen, D. Clark, H. Perez, and H. Huo. 2021. Effects of maternal environment on seed germination and seedling vigor of Petunia× hybrida under different abiotic stresses. Plants. 10: 581.
Nichols, M. A., and W. Heydecker. 1986. Two approaches to the study of germination date. Proc. Int. Seed Test. 33: 531-540.
Nikobin, M., A. Soltani, A. Faraji, and D.F. Mirdavar. 2009. Effect of sowing date at seed filling period on canola (Brassica Napus) seed vigor. J. Plant Prod. 16: 87-100.
Ostadian Bidgoli, R., H.R. Balouchi, E. Soltani, and A. Moradi. 2017. Effects of temperature and water potential on seed germination characteristics in Safflower (Carthamus tinctorius L.) Sofeh var. Iranian J. Seed Sci. Technol. 6: 11-22. (In Persian, with English Abstract)
Patterson, B., E. Macrae. and I. Ferguson. 1984. Estimation of hydrogen peroxide in plant extracts using titanium (IV). Annu. Biochem. 139: 487-492.
Rahmani, V., M Movahhedi Dehnavi, H.R. Balouchi, A. R. Yadavi, and M. Hamidian. 2022. Silicon can improve nutrient uptake and performance of black cumin under drought and salinity stresses. Commun. Soil Sci. Plant Anal, DOI: 10.1080/00103624.2022.2112590
Rajjou, L., M. Duval, K. Gallardo, J. Catusse, J. Bally, C. Job, and D. Job. 2012. Seed germination and vigor. Annu. Rev. Plant Biol. 63: 507-533.
Reed, R.C., K.J. Bradford, and I. Khanday. 2022. Seed germination and vigor: ensuring crop sustainability in a changing climate. Heredity. 1-10.
Rios, J.J., M.C. Martínez-Ballesta, J.M. Ruiz, B. Blasco, and M. Carvajal. 2017. Silicon-mediated improvement in plant salinity tolerance: the role of aquaporins. Front. Plant Sci. 8: 948.
Sadeghi, H., H.H. Sharifabad, A. Hamidi, G. Nourmohammadi, and H. Madani. 2017. Evaluation the effects of mother plant planting date and density on germination and vigor of soybean seed. Seed Sci. Technol. 6: 219-233.
Salehi, M., and S. Pourdad. 2021. Evaluation and selection of safflower (Carthamus tinctorius L) genotypes under salinity stress conditions. Seed and Plant. 37: 83-102
Shahzad, S., S. Ali, R. Ahmad, S. Ercisli, and M.A. Anjum. 2022. Foliar application of silicon enhances growth, flower yield, quality and postharvest life of tuberose (Polianthes tuberosa L.) under saline conditions by improving antioxidant defense mechanism. Silicon. 14: 1511-1518.
Soleymanifard, A., M. Mojaddam, S. Lack, and M. Alavifazel. 2022. Effect of azotobacter chroococcum and nitrogen fertilization on some morphophysiological traits, grain yield, and nitrogen use efficiency of safflower genotypes in rainfed conditions. Commun. Soil Sci. Plant Anal. 53 (6): https://doi.org/10.1080/00103624.2022.2028815.
Souri, Z., K. Khanna, N. Karimi, and P. Ahmad. 2021. Silicon and plants: current knowledge and future prospects. J. Plant Growth Regul..40: 906-925.
Sun, Y., J. Xu, X. Miao, X. Lin, W. Liu, and H. Ren. 2021. Effects of exogenous silicon on maize seed germination and seedling growth. Sci. Rep. 11: 1-13.
Wang, C., Z. Wei, M. Feng, L. Wang, and Z. Wang. 2014. The effects of hydrox-ylated multiwalled carbon nanotubes on the toxicity of nickel to Daphnia magna under different pH levels. Environ. Toxicol. Chem. 33: 2522–2528.
Wu, H., X. Zhang, J.P. Giraldo, and S. Shabala. 2018. It is not all about sodium: revealing tissue specificity and signalling roles of potassium in plant responses to salt stress. Plant and soil. 431: 1-17.
Yadav, S. P., R. Bharadwaj, H. Nayak, R. Mahto, R. K. Singh, and S. K. Prasad. 2019. Impact of salt stress on growth, productivity and physicochemical properties of plants: Int. J. Chem. Stud. 7: 1793-1798.
Yeilaghi, H., A. Arzani, and M. Ghaderian. 2015. Evaluating the contribution of ionic and agronomic components toward salinity tolerance in safflower. Agron. J. 107:  2205-2212.
Yusuf, M., Q. Fariduddin, S. Hayat, and A. Ahmad. 2011. Nickel: an overview of uptake, essentiality and toxicity in plants. Bull. Environ. Contam. Toxicol. 86: 1–17.
Zorb, C., CM. Geilfus, and KJ. Dietz. 2019. Salinity and crop yield. Plant Biol. 21: 31-38.