Document Type : Original Article

Authors

1 Research Assistant Professor, Seed and Plant Certification and Registration Research Institute (SPCRI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.

2 Research Associate Professor, Agricultural Research, Education and Extension Organization (AREEO), Seed and Plant Certification and Registration Institute (SPCRI), Karaj, Iran

3 Master of Seed Laboratory of Agricultural Research, Seed and Plant Certification and Registration Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.

Abstract

Randomized factorial design was implemented in four replications to determine optimal methods of Ajwain (Carum capticum) standard seeds germination. Ajwain seeds were placed top and between paper and sand exposed to two potassium nitrate (two grams per 1000 ml distilled water) and pre-chilling (seven days under 7-10ºC) pre-treatments which were under six temperatures (10, 15, 20, 25, 20-30, and 35ºC) in germinator for 25 days so as to germination indices were assessed. Ajwain seeds viability was estimated 75% after tetrazolium test. The highest final and normal seedling percentages were found under 15 and 20ºC in pre-chilling treatment and also 20ºC in potassium nitrate and control. No germination was observed under 35ºC in potassium nitrate and control, however, pre-chilling led to >26% germination. The higher germination potential was shown in top and between papers compared with sand. The lowest germination rate was recorded in control and potassium nitrate and the highest one in pre-chilling. The maximum of seedling weight vigor was suggested in top and between paper when seeds were put under 10, 15, and 20ºC. In order to evaluate optimal standard germination of Ajwain seeds, it was resulted that applying of two potassium nitrate and pre-chilling treatments placed top and between papers under 15 and 20ºC caused maximum of germination potential and seedling weight vigor.

Keywords

Abdel-Baki, G. K., Shaddad, M., Mostafa, D., & Rafat, A. S. (2018). The effect of seed presoaking with KNO3 on seed germination, proline, protein pattern, ß-amylase, and mineral composition of two faba bean cultivars treated with NaCl. Egyptian Journal of Botany, 58, 445–461. https://doi.org/10.21608/ejbo.2018.3423.1166
Afzali Group, S., Mehdinejad, N., Azad Ghojeh Biglou, H., & Salarnia, N. (2018). The effect of chilling and leaching on removing dormancy from the seeds of Lovage (Levisticum officinale KOCH). Journal of Seed Research, 8, 60-68. [In Persian]
Aihua, L., Shunyuan, J., Guang, Y., Ying, L., Na, G., Tong, C., Liping, K., & Luqi, H. (2018). Molecular mechanism of seed dormancy release induced by fluridone compared with cold stratification in Notopterygium incisum. BMC Plant Biology, 18, 1-16. https://doi.org/10.1186/s12870-018-1333-2
Ali, M. M., Javed, T., Mauro, R. P., Shabbir, R., Afzal, I., & Yousef, A. F. (2020). Effect of seed priming with potassium nitrate on the performance of tomato. Agriculture, 10(11), 1–10. https://doi.org/10.3390/agriculture10110498
Alizadeh, M. A., Hosienpoor Ghazvini, A. A., Jafari, A., & Daneshian, J. (2017). Effect of different treatments on removing seed dormancy to induce seed emergence and vigor in some populations of four species of savory (Satureja spp.). Iranian Journal of Seed Science and Technology, 5, 223-233. https://doi.org/10.22034/ijsst.2017.108295 [In Persian]
Anonymous. (2022). ISTA: International Rules for Seed Testing. International Seed Testing Association.
Anonymous. (1983). Seed vigor testing handbook. Contribution No. 32 to the handbook on Seed Testing. Association of Official Seed Analysis (AOSA).
Baskin, C. C., & Baskin, J. M. (2014). Seeds: Ecology, biogeography, and evolution of dormancy and germination (2nd ed.). Elsevier/Academic Press.
Bewley, J. D., Bradford, K. J., Hilhorst, H. W. M., & Nonagaki, H. (2013). Seeds: Physiology of development, germination and dormancy (3rd ed.). Springer.
Boskabady, M. H., Alitaneh, S., & Alavinezhad, A. (2014). Carum copticum L.: A herbal medicine with various pharmacological effects. BioMed Research International, 2014, 569087. https://doi.org/10.1155/2014/569087
Chen, S. Y., Chou, S. H., Tsai, C. C., Hsu, W. Y., Baskin, C. C., Baskin, J. M., & Kuo-Huang, L. L. (2015). Effects of moist cold stratification on germination, plant growth regulators, metabolites, and embryo ultrastructure in seeds of Acer morrisonense (Sapindaceae). Plant Physiology and Biochemistry, 94, 165-173. https://doi.org/10.1016/j.plaphy.2015.06.004
Cortés-Fernández, I., Cerrato, M. D., & Ribas-Serra, A. (2021). Evidence of interpopulation variation in the germination of Eryngium maritimum L. (Apiaceae). Plant Ecology, 222, 1101–1112. https://doi.org/10.1007/s11258-021-01164-y
Ghaderi-Far, F., & Soltani, A. (2014). Seed testing and control. Publications of University of Mashhad, Mashhad, Iran. [In Persian]
Ghavam, M., Soleimani Nejad, Z., & Tavili, A. (2018). Dormancy breaking of Ducrosia anethifolia Boiss seed under the influence of different treatments. New Cellular and Molecular Biotechnology Journal, 8, 35-44. https://doi.org/20.1001.1.22285458.1397.8.30.4.9 [In Persian]
Gholami, M., Danesh Shahraki, A., Asadi, E., Tahmasbi, P., & Shirmardi, H. (2019). The effects of pre-chilling and gibberellic acid on seed dormancy break, germination, and plant growth indices of Smyrnium cordifolium BOISS. Journal of Rangeland, 13(4), 571-583. https://doi.org/20.1001.1.20080891.1398.13.4.4.1 [In Persian]
Hammami, H., Saadatian, B., & Aliverdi, A. (2018). Geographical variation in breaking the seed dormancy of Persian cumin (Carum carvi L.) ecotypes and their physiological responses to salinity and drought stresses. Industrial Crops and Products, 124, 600-606. https://doi.org/10.1016/j.indcrop.2018.08.040
Kamali, N., & Sadeghipour, A. (2016). Investigation of some dormancy breaking treatments on germination percentage and rate of seeds of Bunium persicum. Watershed Management Research, 29, 24-32. https://doi.org/10.22092/WMEJ.2016.112318
Keshtkar, H. R., Azarnivand, H., Etemad, V., & Moosavi, S. S. (2008). Seed dormancy-breaking and germination requirements of Ferula ovina and Ferula gummosaDesert, 13(1), 45-51. https://doi.org/10.22059/JDESERT.2008.27174
Moaaz Ali, M., Javed, T., Mauro, R. P., Shabbir, R., Afzal, I., & Yousef, A. F. (2020). Effect of seed priming with potassium nitrate on the performance of tomato. Agriculture, 10(11), 498. https://doi.org/10.3390/agriculture10110498
Nkomo, M., & Kambizi, L. (2009). Effects of pre-chilling and temperature on seed germination of Corchorus olitorius L. (Tiliaceae) (Jew’s Mallow), a wild leafy vegetable. African Journal of Biotechnology, 8(6), 1078-1081.
Nowruzian, A., Masoumian, M., Ebrahimi, M. A., & Bakhshi Khaniki, G. A. (2017). Effect of breaking dormancy treatments on germination of Ferula assa foetida L. seed. Iranian Journal of Seed Research, 2, 155-169. https://doi.org/10.29252/yujs.3.2.155 [In Persian]
Nurulla, M., Baskin, C. C., Lu, J. J., Tan, D. Y., & Baskin, J. M. (2014). Intermediate morpho-physiological dormancy allows for life-cycle diversity in the annual weed, Turgenia latifolia (Apiaceae). Australian Journal of Botany, 62, 630-637. https://doi.org/10.1071/BT14281
Orchard, T. (1977). Estimating the parameters of plant seedling emergence. Seed Science and Technology, 5, 61-69.
Rahimi, H., Torabi, B., Soltani, E., & Ghaderi-Far, F. (2017). Investigation of the process of seed germination of wild mustard (Sinapis arvensis) during dormancy elimination. Weed Research Journal, 2, 15-30.
[In Persian]
Raisi, A., Kalat, S. N., & Darban, A. S. (2013). The effects of stratification, temperature, and potassium nitrate on seed dormancy breaking in Ferula assa foetidaWorld Applied Sciences Journal, 23, 379-383. https://doi.org/10.5829/idosi.wasj.2013.21.3.2785
Rezazadeh, Z. B., & Koocheki, A. (2006). Evaluation of cardinal temperatures for three species of medicinal plants, Ajowan (Trachyspermum ammi), fennel (Foeniculum vulgare), and dill (Anethum graveolens). Desert Journal, 11(2), 11-16. https://doi.org/10.22059/JDESERT.2006.31870
Sasani, S., Tavakol-Afshari, R., Postini, K., & Sharifzadeh, F. (2006). Evaluation of the effect of moist-chilling, hormonal treatments, and storage period on dormancy breaking and induction of Bunium persicum (Boiss.) B. Fedtsch seed germination [Abstract]. In Proceedings of the 9th Iranian Congress of Plant Science and Plant Breeding. 27 August 2006, Tehran, Iran. [In Persian]
Zahin, M., Ahmad, I., & Aqil, F. (2010). Antioxidant and antimutagenic activity of Carum copticum fruit extracts. Toxicology in Vitro, 24(4), 1243-1249. https://doi.org/10.1016/j.tiv.2010.02.004
Zardari, S., Ghaderi-Far, F., Sadeghipour, H. R., Zeinali, E., Soltani, E., & Baskin, C. C. (2019). Deep and intermediate complex morpho-physiological dormancy in seeds of Ferula gummosa (Apiaceae). Plant Species Biology, 34, 85-94. https://doi.org/10.1111/1442-1984.12238
Zhang, K., Zhang, Y., Walck, J. L., & Tao, J. (2019). Non-deep simple morpho-physiological dormancy in seeds of Angelica keiskei (Apiaceae). Scientia Horticulturae, 255, 202-208. https://doi.org/10.1016/j.scienta.2019.05.039