Document Type : Original Article
Authors
1 Faculty member Seed and Plant Certification and Registration Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
2 Researcher of Seed and Plant Certification and Registration Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
3 - Faculty member Seed and Plant Certification and Registration Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
4 Seed and Plant Certification and Registration Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
Abstract
Genetic purity, as one of the important characteristics of the quality of seed lots, has been considered in the national standards of certified seed producing countries to achieve desirable and stable characteristics of agricultural cultivars. According to the national standard, determining the other speciese in the seed sample is considered as one of the important tests of seed quality. This feature is checked with an emphasis on recognizable characteristics in the seed analysis laboratory. The research has investigated the characteristics and differences in appearance and genetics of seeds off- type of chickpea seeds. The results showed that the seeds that were considered as off- type morphologically differed from the control in at least one of the measured traits. These seeds were different from the original seed in terms of color, shape seed surface roughness and seed groove, also the selected indices were similar in the first and second generation seeds. The results of the molecular test also confirmed the results of the morphological investigations. In Adel, Mansour, Arman cultivars, the samples that in morphological studies were considered as off-type, in molecular studies using microsatellite markers H3F09, H3C11 and H1A06 showed that the molecular profile was not uniform and allele diversity was greater than the main allele of cultivars. In the process of seed certification program, microsatellite markers are a suitable and complementary tool to the use of morphological markers to determine the genetic purity of chickpea seed samples.
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