The Effect of Different Dormancy Breaking Treatments on Germination of Sophora Seeds (Sophora alopecuroides L.)

Kalantari, Hadi; Izady Darbandi, Ali; Qaderi, Ardeshir; Sadat Noori, Seyed Ahmad; Labbafi, Mohammadreza; Ahvazi, Maryam

doi:10.22092/ijsst.2024.364188.1508

Document Type : Original Article

Authors

¹ Department of Agronomy and Plant Breeding Science, College of Aburaihan, University of Tehran, Tehran, Iran.

² Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR,, Karaj, Iran.

³ Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran.

https://doi.org/10.22092/ijsst.2024.364188.1508

Abstract

The legume family has seed dormancy, which is an evolutionary adaptation that prevents seed germination in adverse ecological conditions and it is very important in preserving plant species. In order to study the effect of various mechanical, hormonal, and physical factors on the dormancy breaking of sophora seeds, an experiment was conducted in the form of a completely randomized design in three replications. The treatments included gibberellic acid, sulfuric acid, hot water, and cold at 4 degrees Celsius in different periods. The results showed that Compared to the very low germination of untreated seeds (0-53%), the application of 75% concentration of sulfuric acid for 15 minutes, improved the germination percentage (95.5%) and also the initial growth of the seedling. The percentage of germination (93%) in the treatment having hot water was significant compared to the control sample (56%). Compared to the very low germination of untreated seeds (0-53%), sulfuric acid (73-95.5%) and soaking in hot water (52-93%) were effective in germination. According to the dormancy failure of bitter gourd seeds in the tested treatments, it can be concluded that the dormancy of bitter gourd seeds is of a mechanical type and related to the physical properties of the seed coat. treatments of hot water and l sulfuric acid can remove seed dormancy in sophora. According to the breaking sophora seeds dormancy in the tested treatments, it can be concluded that the seed dormancy is of a mechanical type and related to the physical properties of the seed coat.

Keywords

References

Balouchi, H. R., Modares, S. S., & Bakhtiar, A. Z. (2008). Effective factors on seed dormancy and germination of two annual medics. Iranian Journal of Biology, 21(2), 261–270. [In Persian]

Barbosa, D., Gealdo, M. O., Alvarenga, M., Matovani, E., & Sants, F. D. (2005). Effect of acid scarification and different temperatures on physiological quality of Strelitzia reginase seeds. Revista Brasileira de Sementes, 27, 71–77. https://doi.org/10.1590/S0101-31222005000100009 [In Portuguese]

Baskin, C. C., & Baskin, J. M. (1998). Seeds: Ecology, biogeography and evaluation of dormancy and germination. Academic Press.

Baskin, C. C., Milberg, P., Andersson, L., & Baskin, J. M. (2004). Germination ecology of seeds of the annual weeds Capsella bursa‐pastoris and Descurainia sophia originating from high northern latitudes. Weed Research, 44(1), 60–68. https://doi.org/10.1046/j.1365-3180.2003.00373.x

Baskin, C. C., Zackrisson, O., & Baskin, J. M. (2002). Role of warm stratification in promoting germination of seeds of Empetrum hermaphroditum (Empetraceae), a circumboreal species with a stony endocarp. American Journal of Botany, 89(3), 486–493. https://doi.org/10.3732/ajb.89.3.486

Baskin, J. M., Baskin, C. C., & Li, X. (2000). Taxonomy, anatomy and evolution of physical dormancy in seeds. Plant Species Biology, 15(2), 139–152. https://doi.org/10.1046/j.1442-1984.2000.00034.x

Bayat, P., Ghobadi, M., Qobadi, M. I., & Mohammadi, G. H. (2016). Evaluation of the ability of standard seed germination test in laboratory conditions to predict the emergence and establishment of chickpea (Cicer arietinum L.) seedlings in the field. Iranian Journal of Seed Science and Technology, 5, 27–38. [In Persian]

Benech Arnold, R. L., Fenner, M., & Edwards, P. J. (1991). Changes in germinability, ABA content and ABA embryonic sensitivity in developing seeds of Sorghum bicolor (L.) Moench induced by water stress during grain filling. New Phytologist, 118(2), 339–347. https://doi.org/10.1111/j.1469-8137.1991.tb00986.x

Benech-Arnold, R. L., Sánchez, R. A., Forcella, F., Kruk, B. C., & Ghersa, C. M. (2000). Environmental control of dormancy in weed seed banks in soil. Field Crops Research, 67(2), 105–122. https://doi.org/10.1016/S0378-4290(00)00087-3

Bewley, J. D., Bradford, K., & Hilhorst, H. (2013). Seeds: Physiology of development, germination and dormancy (3rd ed.). Springer Science and Business Media.

Bhatt, A., Gairola, S., & El-Keblawy, A. A. (2016). Seed colour affects light and temperature requirements during germination in two Lotus species (Fabaceae) of the Arabian subtropical deserts. Revista de Biología Tropical, 64(2), 483–492. https://doi.org/10.15517/rbt.v64i2.18575

Cavallaro, V., Maucieri, C., Patane, C., Fascella, G., Fascella, A., & Barbera, A. C. (2021). Polyphenols leaching and seed dormancy in carob (Ceratonia siliqua L.) in relation to hot water treatment. Acta Physiologiae Plantarum, 43, 1–10. https://doi.org/10.1007/s11738-021-03308-z

Chien, C. T., Chen, S. Y., Baskin, J. M., & Baskin, J. C. (2011). Morphophysiological dormancy in seeds of the ANA grade angiosperm Schisandra arisanensis (Schisandraceae). Plant Species Biology, 26(1), 99–104. https://doi.org/10.1111/j.1442-1984.2010.00299.x

Dayrell, R. L. C., de Jesus Gonçalves-Alvim, S., Negreiros, D., Fernandes, G. W., & Silveira, F. A. O. (2015). Environmental control of seed dormancy and germination of Mimosa calodendron (Fabaceae): Implications for ecological restoration of a highly threatened environment. Brazilian Journal of Botany, 38, 395–399. https://doi.org/10.1007/s40415-015-0145-y

Delač, D., Gršić, K., Ninčević, T., Carović-Stanko, K., Varga, F., & Grdiša, M. (2018). The influence of hydropriming and osmopriming with KNO₃ on seed germination of Dalmatian pyrethrum (Tanacetum cinerariifolium (Trevir.) Sch. Bip.). Agriculturae Conspectus Scientificus, 83(3), 205–211.

Deng, X., Wang, R., Gao, Q., Wu, X., Han, L., Gao, X., & Bai, C. (2020). Sophora alopecuroides L.: An ethnopharmacological, phytochemical, and pharmacological review. Journal of Ethnopharmacology, 248, 112172. https://doi.org/10.1016/j.jep.2019.112172

Esechie, H. A. (1994). Interaction of salinity and temperature on the germination of sorghum. Journal of Agronomy and Crop Science, 172(3), 194–199. https://doi.org/10.1111/j.1439-037X.1994.tb00166.x

Fattahi, M., Nazeri, V., Sefidkon, F., Zamani, Z., & Palazon, J. (2011). The effect of pre-sowing treatments and light on seed germination of Dracocephalum kotschyi Boiss: An endangered medicinal plant in Iran. Horticulture, Environment, and Biotechnology, 52, 559–566. https://doi.org/10.1007/s13580-011-0057-0

Fox, C. W., Wallin, W. G., Bush, M. L., Czesak, M. E., & Messina, F. J. (2012). Effects of seed beetles on the performance of desert legumes depend on host species, plant stage, and beetle density. Journal of Arid Environments, 80, 10–16. https://doi.org/10.1016/j.jaridenv.2011.12.008

Funes, G., & Venier, P. (2006). Dormancy and germination in three Acacia (Fabaceae) species from central Argentina. Seed Science Research, 16(1), 77–82. https://doi.org/10.1079/SSR2005229

García, F. J. P. (2009). Germination characteristics and intrapopulation variation in carob (Ceratonia siliqua L.) seeds. Spanish Journal of Agricultural Research, 7(2), 398–406. https://doi.org/10.5424/sjar/2009072-431 [In Spanish]

Ghadiri, H., & Bagherani, T. N. (2000). Effects of scarification and temperature on germination of licorice (Glycyrrhiza glabra L.). Iranian Journal of Seed Science and Technology, 2, 257–262. [In Persian]

Hatami, M., Samadi, M., & Khanizadeh, P. (2019). Effect of different treatments on breaking seed dormancy and stimulating germination in dragonhead (Dracocephalum kotschyi Boiss). Integrated Research on Disaster Risk, 26, 918–931. [In Persian]

Ibrahim, A., & Freat, U. (2001). The effects of some applications on germination rate of Gelemen clover seeds gathered from natural vegetation in Samsun. Pakistan Journal of Biological Sciences, 4(2), 181–183.

Khaleghi, E., Dehghan, A., & Moalemi, N. (2009). The effects of sulfuric acid and hot water treatment on the germination indices of tamarind and acacia seeds. International Journal of Horticultural Science and Technology, 40, 71–77. [In Persian]

Koornneef, M., Bentsink, L., & Hilhorst, H. (2002). Seed dormancy and germination. Current Opinion in Plant Biology, 5, 33–36. https://doi.org/10.1016/S1369-5266(01)00219-9

Kucera, B., Cohn, M. A., & Metzger, G. L. (2005). Plant hormone interactions during seed dormancy release and germination. Seed Science Research, 15, 281–307. https://doi.org/10.1079/SSR2005218

Labouriau, L. G. (1983). Uma nova linha de pesquisa na fisiologia da germinação das sementes. In Anais do XXXIV Congresso Nacional de Botânica (pp. 11–50). SBB.

Lavoie, J. M., Bare, W., & Bilodeau, M. (2011). Depolymerization of steam-treated lignin for the production of green chemicals. Bioresource Technology, 102(7), 4917–4920. https://doi.org/10.1016/j.biortech.2011.01.010

Mao, P. S., Wang, Y. H., Wang, X. G., Lian, J. J., & Huang, Y. (2008). Conditions and stimulation for germination in Glycyrrhiza uralensis Fisch seeds. Agricultural Sciences in China, 7(12), 1438–1444. https://doi.org/10.1016/S1671-2927(08)60400-9

Mehrabi, A. A., & Hajinia, S. (2019). The effect of seed pre-treatments on germination of Astragalus gossypinus seed. Iranian Journal of Seed Research, 6, 95–113. [In Persian]

Mousavi, S. M., Omidi, H., & Mousavi, S. E. (2022). The effect of biological pre-treatments on germination, growth, and physiological indices of fenugreek (Trigonella foenum-graecum L.) seedlings under natural salinity stress. Iranian Journal of Seed Science and Technology, 11(1), 131–145. https://doi.org/10.22092/ijsst.2021.355229.1400 [In Persian]

Musavi, S. H., Motaez, M., Zamiri-Akhlaghi, A., Emami, S. A., & Tayarani-Najaran, Z. (2014). In vitro evaluation of cytotoxic and apoptogenic properties of Sophora pachycarpa. The IJ Pharmaceutical Research, 13(2), 665–673.

Nosratti, I., Amiri, S., Bagheri, A., & Chauhan, B. S. (2018). Environmental factors affecting seed germination and seedling emergence of foxtail sophora (Sophora alopecuroides). Weed Science, 66(1), 71–77. https://doi.org/10.1017/wsc.2017.35

Odoemena, C. S. (1988). Breaking of seed coat dormancy in a medicinal plant Tetrapleura tetraptera (Schum. & Thonn.). The Journal of Agricultural Science, 111(2), 393–394. https://doi.org/10.1017/S0021859600083349

Orchard, T. J. (1977). Estimating the parameters of plant seedling emergence. Seed Science and Technology, 5, 61–69.

Radsarian, R., Karamshahi, A. A., Mirzaei, J., & Heidari, M. (2018). The effect of different chemical and physical treatments on the seed germination of Crataegus pontica C. Koch. Iranian Journal of Seed Research, 4, 1–12. [In Persian]

Ranal, M. A., Santana, D. G. D., Ferreira, W. R., & Mendes-Rodrigues, C. (2009). Calculating germination measurements and organizing spreadsheets. Brazilian Journal of Botany, 32, 849–855. https://doi.org/10.1590/S0100-84042009000400022

Scott, S. J., Jones, R. A., & Williams, W. (1984). Review of data analysis methods for seed germination. Crop Science, 24(6), 1192–1199. https://doi.org/10.2135/cropsci1984.0011183X002400060043x

Shao, L., Liu, X. Q., Gituru, R. W., Ruan, R., & Chen, L. Q. (2010). Effect of pretreatment on breaking hard seed dormancy of Sophora davidii (Franch.) Skeels, a native Chinese shrub with potential for vegetation restoration in degraded habitats. Seed Science and Technology, 38(3), 792–796. https://doi.org/10.15258/sst.2010.38.3.28

The Plant List. (2013). A working list of all plant species. Available online at: http://www.theplantlist.org/tpl1.1/search?q=Sophora

Van Veldhuizen, B., & Knight, C. (2006). Dragonhead mint Dracocephalum parviflorum Nutt. as a potential agronomic crop for Alaska. Agricultural and Forestry Experiment Station, School of Agriculture and Land Resources Management, University of Alaska Fairbanks.

Wang, J., Lü, B., Xiao, Z., Yan, J., & Zhang, Y. (2010). Seed characteristics and hard seed broken methods of Sophora alopecuroides and Thermopsis lanceolata. Transactions of the Chinese Society of Agricultural Engineering, 26(1), 406–410.

Wang, P., Mo, B., Long, Z., Fan, S., Wang, H., & Wang, L. (2016). Factors affecting seed germination and emergence of Sophora davidii. Industrial Crops and Products, 87, 261–265. https://doi.org/10.1016/j.indcrop.2016.04.053

Yang, L., Wang, Y., & Hu, X. W. (2021). Effect of temperature and moisture on physical dormancy release in two populations of Sophora alopecuroides. International Grassland Congress Proceedings, 2, 613.

The Effect of Different Dormancy Breaking Treatments on Germination of Sophora Seeds (Sophora alopecuroides L.)

References

References

Volume 14, Issue 1 - Serial Number 38Spring of 2025March 2025Pages 17-31

Volume 14, Issue 1 - Serial Number 38
Spring of 2025
March 2025
Pages 17-31