Document Type : Original Article

Authors

1 Professor, Department of Plant Production and Genetics, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran.

2 Ph.D Graduate, Crop Physiology, Department of Plant Production and Genetics, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran.

3 Postdoctoral Researcher, School of Integrated Circuits, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, P.R. China.

Abstract

Developing new strategies such as using nanotechnology to control weeds is one of the ways to reduce the environment from improper use of chemical inputs. For this purpose, in 2024, a factorial experiment was conducted based on a completely randomized design with three replications on the germination of Redroot pigweed (Amaranthus retroflexus) seeds. Experimental treatments include aqueous extract of Sunn hemp (in concentrations of 0, 10, 15, and 20 g/L-1) and TiO2 NPs synthesized with aqueous extract of Sunn hemp (TiO2 NPs synthesized without Sunn hemp extract (Control), TiO2 NPs synthesized with 10, 15, and 20 g/L-1 Sunn hemp aqueous extract). Green synthesis of TiO2 NPs with Sunn hemp compared to Sunn hemp aqueous extract at concentrations of 10 and 15 g/L-1 decreased germination by 30.56 and 43.01%. This study indicated that the TiO2 NPs synthesized with Sunn hemp extract caused a 1.20 and 12.63 % increase in lipid peroxidation and decreased catalase enzyme activity by 3.84% and 5.72% in concentrations of 10 and 15 g/L-1, compared to Sunn hemp aqueous extract. This experiment showed a strong inhibition of green synthesis of TiO2 NPs using Sunn hemp aqueous extract on germination and related components in Redroot pigweed seeds.

Keywords

Ahmadnia, F., Alebrahim, M.T., Nabati Souha, L., & MacGregor, D.R. (2023b). Evaluation of techniques to break seed dormancy in Redroot pigweed (Amaranthus retroflexus). Food Science and Nutrition, 00,1-12. DOI: 10.1002/fsn3.3920
Ahmadnia, F., Ebadi, A., & Hashemi M., & Nabati, L. (2020). Investigating the effectiveness of Sunn Hemp (Crotalaria juncea) and Rye (Secale cereal L.) in weed suppression and yield of Kohlrabi (Brassica oleracea var. Gongylodes). Journal of Agricultural Science and Sustainable Production, 31(2), 43-56. DOI: 10.22034/saps.2021.13090 [In Persian]
Ahmadnia, F., Ebadi, A., Alebrahim, M.T., Parmoon, G., Feizpoor, S., & Hashemi, M. (2025). The efficacy of Sunn Hemp (Crotalaria juncea) and Fe3O4 nanoparticles in controlling weed seed germination. Agronomy, 15(4), 795, https://doi.org/10.3390/agronomy15040795
Ahmadnia, F., Ebadi, A., Hashemi, M., Ghavidel, A., & Alebrahim, M.T. (2022). The effect of Sunn hemp extract (Crotalaria juncea) formulated with chitosan nanoparticle on the germination of some weeds. Journal of Seed Research, 12 (4), 11-28. DOI: 10.30495/jsr.2023.1988281.1257 [In Persian]
Ahmadnia, F., Ebadi, A., Hashemi, M., Ghavidel, A., & Alebrahim, M.T. (2023a). Investigating the effect of aqueous extracts of sunn hemp (Crotalaria juncea) and oats (Avena sativa L.) on the germination of wild mustard weed (Sinapis arvensis). Iranian Journal of Seed Science and Research, 10(2), 1-19. DOI: 10.22124/jms.2023.7605 [In Persian]
Bais, H.P., Weir, T.L., Perry, L.G., Gilroy, S., & Vivanco, J.M. (2006). The role of root exudates in rhizosphere interactions with plants and other organisms. Annual Review of Plant Biology, 57, 233-266. DOI: 10.1146/annurev.arplant.57.032905.105159
Baiyeri, K.P., Ugese, F.D., & Uchendu, T.O. (2011). The effect of pervious treatments on passion fruit seed quality, and seedling emergence and growth qualities in soilless media. Journal of Agricultural Science and Technology, 5, 1397-1407. DOI: 10.20546/ijcmas.2017.604.077
Chauhan, B.S., & Gill, GS. (2014). Ecologically based weed management strategies. Recent Advance in Weed Management: 1-11components. Journal of Agroecology, 5(2), 64-74. DOI: 10.1007/978-1-4939-1019-9_1
Cho, A.H., Chase, C.A., & Treadwell, D.D. (2015). Apical dominance and planting density effects on weed suppression by Sunn Hemp (Crotalaria juncea L.). Hortscience, 50(2), 263-267. DOI: https://doi.org/10.21273/HORTSCI.50.2.263
Choudhary, S.K., Kumar, A., & Kumar, R. (2020). Novel nanotechnological tools for weed management - A Review. Chemical Science Review and Letters, 9(36), 886-894. DOI: 10.37273/chesci.CS205105136
Colegate, S.M., Gardner, D.R., Joy, R.J., Betz, J.M. & Panter, K.E. (2012). Dehydropyrrolizidine alkaloids, including monoesters with an unusual esterifying acid, from cultivated Crotalaria juncea (Sunn hemp cv. ‘Tropic Sun’). Journal of Agricultural and Food Chemistry, 60, 3541-3550. DOI: 10.1021/jf205296s
Costea, M., Weaver, SE., & Tardif, FJ. (2004). The biology of Canadian weeds. 130. Amaranthus retroflexus L., A. powellii S. Watson and A. hybridus L. Canadian Journal of Plant Science, 84, 631-668. https://doi.org/10.4141/P02-183
Cristaudo, A., Gresta, F., Luciani, F., & Restuccia, A. (2007). Effects of after-harvest period and environmental factors on seed dormancy of Amaranthus species. European Weed Research Society Weed Research, 47, 327-334. DOI: 10.1111/j.1365-3180.2007. 00574.x
Dayem, A. A., Hossain, M.K., Lee, S.B., Kim, K., Saha, S.K., Yang, G., Choi, H.Y., & Cho, S.G. (2017). The role of reactive oxygen species (ROS) in the biological activities of metallic nanoparticles. International Journal of Molecular Science, 18(1), 120. https://doi.org/10.3390/ijms18010120.
Ebadi, A., Feizpoor, S., & Ahmadnia, F. (2025). Investigating the impact of sunflower (Helianthus annuus) allelopathic aqueous extract in the green synthesis by titanium dioxide nanoparticles on wild mustard (Sinapis arvensis L.) seed germination. Journal of Plant Process and Function Iranian Society of Plant Physiology, 14(66), 47-64. DOI: 10.22034/14.66.47 [In Persian]
Ellis, R.H. (1992). Seed and seedling vigor in relation to crop growth and yield. Plant Growth Regulation, 11, 249-255. http://dx.doi.org/10.1007/BF00024563
Enayati, V., Esfandiari, E., Pourmohammad, A., & Haj Mohammadnia Ghalibaf, K. (2019). Evaluation of different methods in seed dormancy breaking and germination of redroot pigweed (Amaranthus retroflexus). Iranian Journal of Seed Research, 5(2), 129-137. DOI:10.29252/yujs.5.2.129 [In Persian]
Eskandarinasab, M., Rafieiolhossaini, M., Roshandel, P., & Tadayon, M.R. (2019). Investigation of seed germination indices and anthocyanin content of Niger (Guizotia abyssinica) seedling under the effect of three nanoparticles. Iranian Journal of Seed Research, 5(2), 73-89. DOI:10.29252/yujs.5.2.73 [In Persian]
Fatima, S., Ali, K., Ahmed, B., Al Kheraif, A.A., Syed, A., Elgorban, A.M., Musarrat, J. & Lee, J. (2021). Titanium dioxide nanoparticles induce inhibitory effects against planktonic cells and biofilms of human oral cavity isolates of rothia mucilaginosa, Georgenia sp. and Staphylococcus saprophyticus. Pharmaceutics, 13(1564), 1-15. https://doi.org/10.3390/ pharmaceutics13101564
Garzon, J., Vendramini, J.M.B., Silveira, M.L., Moriel, P., De Silva, H.M.S., Dubeux Jr, J.C.B., Keaneko, M., Carnelos, C.C. & Mamede, P.A. (2020). Harvest management and genotype effects on Sunn hemp forage characteristics. Agronomy Journal, 113(2021), 298-307. https://doi.org/10.1002/agj2.20465
Grand, F., & Tucci, P. (2016). Titanium dioxide nanoparticles: a risk for human health? Mini-Review in Medicinal Chemistry, 16, 762-769. https://doi.org/10.2174/1389557516666160321114341.
Janusauskaite, D. (2023). Allelopathic effect of aqueous extracts of common sunflower on seed germination and growth of field pea. Zemdirbyste-Agriculture, 110(1),17-26. DOI 10.13080/z-a.2023.110.003
Javaid, M.M., Bhan, M., Rathinasabapahi, B., & Chase, C.A. (2012). A non-protein amino acid from Crotalaria juncea foliage with allelopathic potential. American Society for Horticultural Science, Abstract.
Khan, M.H., & Panda, S.K. (2009). Alterations in root lipid peroxidation and antioxidative responses in two rice cultivars under NaCl-salinity stress. Acta Physiologiae Plantarum, 30 (1), 81-89. DOI: 10.1007/s11738-007-0093-7
Khandekar, D.C., Bhattacharyya, A.R., & Bandyopadhyaya, R. (2021). Role of axial versus radial pore orientation in mesoporous silica particles, on its effect in photocatalysis via impregnated TiO2 nanoparticles in pores. Chemical Engineering Journal Advances, 5 (2021), 100075, https://doi.org/10.1016/j.ceja.2020.100075
Macias, F.A., Torres, A., Galindo, J.L.G., Varela, R.M., Alvares, J.A., & Molinillo, J.M.G. (2002). Bioactive terpenoids from sunflower leaves cv. Peredovick. Phytochemistry, 61(6), 687-692. https://doi.org/10.1016/S0031-9422(02)00370-9 
Maguire, J.D. (1962). Speed of germination, aid in selection and evaluation for seedling emergence and vigour. Crop Science, 2,176-177. https://doi.org/10.2135/cropsci1962.0011183X000200020033x
Mahiuddin, MD., Saha, P., & Ochiai, B. (2020). Green synthesis and catalytic activity of silver nanoparticles based on Piper chaba stem extracts. Nanomaterials, 10(9), 1777, 1-15. DOI: 10.3390/nano10091777
Marslin, G., Sheeba, C.J., & Franklin, G. (2017). Nanoparticles alter secondary metabolism in plants via ros burst. Frontiers in Plant Science, 8, 1–8. https://doi.org/10.3389 /fpls.2017.00832
Mokhtari, H., Hosseini, S.Z., & Kazemeni, S.A. (2014). Allelopathic effects of sorghum on Milk Thistle (Silybum marianum L.) seed germination and growth. Research on Crop Ecophysiology, 9(2), 115-123. https://journals.iau.ir/article_533661.html
Morris, JB., Chase, C., Treadwell, D., Koenig, R., Cho, A., Morales-Payan, J.P., Murphy, T., & Antonious, G.F. (2015). Effect of Sunn hemp (Crotalaria juncea L.) cutting date and planting density on weed suppression in Georgia, USA. Journal of Environmental Science and Health, Part B, 50, 614-621. DOI: 10.1080/03601234.2015.1028855
Muhammad, Z., & Majeed, A. (2014). Allelopathic effects of aqueous extracts of sunflower on wheat (Triticum aestivum L.) and maize (Zea mays L.). Pakistan Journal of Botany, 46(5), 1715-1718. https://inis.iaea.org/search/search.aspx?orig_q=RN:46013965
Nafees, A., Abbas, A., Us-Salam, I., & Hussain, F. (2021). Allelopathic effects of sunflower (Helianthus annuus L.) against Luffa cylindrica (L.) Roem. Ghazi University Journal of Phytosciences, 1(2), 124-132. https://jphytosci. com/index.php/GUJP/article/view/29
Nivas, D., Uttam, D., & Dattatraya, G. (2017). Allelopathic effect of Excoecaria agallocha L. mangrove leaf leachate on germination and growth behavior of Eleusine coracana (Finger Millet). American Journal Plant Physiology, 12, 38-44. DOI: 10.3923/ajpp.2017.38.44
Nusrat, A., Agrawal, N., & Kumar, J. (2018). Bio-herbicides for sustainable and eco-friendly weed control: a review. International Journal of Advanced Research, 6(12), 550-561. DOI: 10.21474/IJAR01/8173
Parenti, A., Cappelli, G., Zegada-Lizarazu, W., Sastre, C.M., Christou, M., Monti, A., & Ginaldi, F. (2021). SunnGro: A new crop model for the simulation of sunn hemp (Crotalaria juncea L.) grown under alternative management practices. Biomass and Bioenergy, 146(2021), 1-16. https://doi.org/10.1016/j.biombioe.2021.105975
Perry, D.A. (1991). Methodology and application of vigor tests. International Seed Testing Association. Zurich. Switzerland. 275p.
Pham, X.N., Pham, D.T., Ngo, H.S., Nguyen, M.B., & Doan, H.V. (2021). Characterization and application of C–TiO2 doped cellulose acetate nanocomposite film for removal of Reactive Red-195. Chemical Engineering Communications, 208(3), 304-317. DOI: 10.1080/00986445.2020.1712375
Pula, J., Zandi, P., Stachurska-Swakori, A., Barabasz-Krasny, B., Mozdžen K., & Wang, Y. (2020). Influence of alcoholic extracts from Helianthus annnus L. roots on the photosynthetic activity of Sinapis alba L. cv. Barka plants. Acta Agriculturae Scandinavica, Section B Soil and Plant Science, 70, 8-13. DOI: 10.5281/zenodo.1034526
Qi, Y., Yan, B., Fu, G., Guan, X., Du, L., & Li. J. (2017). Germination of seeds and seedling growth of Amaranthus retroflexus L. following sublethal exposure of parent plants to herbicides. Scientific Reports, 7(157), 1-8. DOI: 10.1038/s41598-017-00153-4
Rigon C. A. G., Salamoni, A., Aguiar, A.C.M., & Cutti, L. (2018). Allelopathic effect of aqueous extracts of different organs of three sunflower cultivars on germination of radish. Bioscience Journal, 34 (3), 577-586.  https://doi.org/10.14393/BJ-v34n3a2018-36771
Romano de Melo, G.S., Constantin, R.P., Abrahão, J., Foletto-Felipe, M.D.P., Constantin, R.P., Dantas dos Santos, W., Ferrarese-Filho, O., & Marchiosi, R. (2021). Titanium dioxide nanoparticles induce root growth inhibition in soybean due to physical damages. Water, Air & Soil Pollution, 232(25), 1-12. http://doi.org./10.1007/s11270-020-04955-7
Sahoo, T.R., Behera, B., Paikaray, R.K., Garnayak, L.M., Sethi, D., Jena, S., Raza, B., Panda, R.K., Song, B., Lal, M.K., & Kumar, A. (2023). Effects of sunflower residue management options on productivity and profitability of succeeding rice under different crop establishment methods. Field Crops Research, 290(2023), 108763. DOI: 10.1016/j.fcr.2022.108763
Sakadzo, N., Pahla, I., Muzemu, S., Mandumbu, R., & Makaza, K. (2018). Thornapple (Datura stramonium L.) allelopathy on cowpeas (Vignaun guiculata L.) and wheat (Triticum aestivum L.) in Zimbabwe. African Journal Agric Research, 13(29), 1460-1467. https://doi.org/10.5897/AJAR2018.13226
Salvadori, M.R., Ando, R.A., Nascimento, C.A.O., & Corea, B. (2014). Bioremediation from wastewater and extracellular synthesis of copper nanoparticles by the fungus Trichoderma koningiopsis. Journal of Environmental Science and Health, 49, 286-1295. DOI: 10.1080/10934529.2014.910067
Sant´as-Miguela, V., Arias-Estevez, M., Rodriuez-Seijo, A., & Arenas-Lago, D. (2023). Use of metal nanoparticles in agriculture. A review on the effects on plant germination. Environmental Pollution, 334(2023), 122222, 1-17. https://doi.org/10.1016/j.envpol.2023.122222
Scott, S.J., Jones, R.A., & Williams, W.A. (1984). Review of data analysis methods for seed germination. Crop Science, 24(6), 1192-1199. https://doi.org/10.2135/cropsci1984.0011183X002400060043x
Skinner, E.M., Diaz-Perez, J.C., & Phatak, Sh.C. (2012). Allelopathic effects of Sunn hemp (Crotalaria juncea L.) on germination of vegetables and weeds. Hortscience, 47(1):138-142. DOI: 10.21273/HORTSCI.47.1.138
Stewart, R.C., & Beweley, J.D. (1980). Lipid peroxidation associated with accelerated aging of soybean axes. Plant Physiology, 65(2), 245-248. DOI: 10.1104/pp.65.2.245
Sudhakar, C., Lakshmi, A., & Giridara Kumar, S. (2001). Changes in the antioxidant enzyme efficacy in two high yielding genotypes of mulberry (Morus alba L.) under NaCl salinity. Plant Science, 167(3), 613-619. DOI: 10.1016/S0168-9452(01)00450-2
Trusheva, B., Trusnkova, D., & Bankova, V. (2007). Different extraction methods of biologically active components from propolis: a preliminary study. Chemistry central Journal, 1(13), 1-4. DOI: 10.1186/1752-153X-1-13
Vangessel, M.J., & Renner, K.A. (1990). Redroot pigweed (Amaranthus retroflexus) and barnyard grass (Echinochloa crusgalli) interference in potatoes (Solanum tuberosum). Weed Science, 38(4, 5), 338-343. https://doi.org/10.1017/s0043174500056642
Wang, K., Zhuo, Y., Chen, J., Gao, D., Ren, Y., Wang, C., & Qi, Z. (2020). Crystalline phase regulation of anatase–rutile TiO2 for the enhancement of photocatalytic activity. RSC advances, 10(71), 43592-43598. DOI: 10.1039/d0ra09421h
Yuan, X., Wang, X., Zhang, Sh., Dong, X., Zhang, Sh., Zhou, W., Zhang, Ch., & Luo, Y. (2021). Synergistic enhancement of photocatalytic performance of mesoporous TiO2 enabled by tunable crystal phase and hybridization with graphene oxide. Chemistry Select, 6(23), 5791-5800. https://doi.org/10.1002/slct.202100784