Document Type : Original Article

Authors

1 MSc student, Seed Science and Technology, Faculty of Agriculture, Yasouj University, Iran.

2 Associate Prof., Dept. of Agronomy and Plant Breeding, Faculty of Agriculture, Yasouj University, Yasouj, Iran.

3 Assistant Prof. Dept. of Agronomy and Plant Breeding, Faculty of Agriculture, Yasouj University, Yasouj, Iran.

4 Professor, Dept. of Agronomy and Plant Breeding, Faculty of Agriculture, Yasouj University, Yasouj, Iran.

Abstract

One of the most important problems of camelina in cold regions is the interaction of seed germination stage and establishment with cold stress, the use of seed priming technology can be effective in this regard. This study aimed to investigate the effect of priming and drying methods on germination and some biochemical characteristics of camelina under low temperature,, research was carried out in the Agronomy and Genetics Laboratory of the Faculty of Agriculture of Yasouj University during 2021-2022. A split factorial experiment was conducted in a completely randomized design with four replications in which the main plot consisted of low temperature at four levels (5, 10, 15 and 20 °C), a sub-factor including priming hormone using salicylic acid (0.25, 0.5, 0.75 and 1 mM) and gibberellic acid (at three levels of 50, 100 and 150 mg/L) and drying method for primed seed was done in two levels (rapid and normal method). It was observed that the drying method and priming treatment affected the biochemical and germination parameters of Camellina. In most of the studied temperatures, priming with gibberellic acid with concentrations of 100 and 150 mg/L with slow drying method had the highest proline content and could improve germination indices of Camellia seed. The most effective method was normal (slow) drying method among drying treatments. Based on the results of the experiment, it can be stated that applying priming with hormones while improving the physical characteristics of the Camellia seed, helps germination under optimum conditions and low temperature.

Keywords

Akhil, R. B., Ishigo-Oka, N., Adachi, M., Oguma, Y., Tokizono, Y., Onishi, K., & Sano, Y. (2008). Cold tolerance at the early growth stage in wild and cultivated rice. Euphytica, 165(3), 459–470. https://doi.org/10.1007/s10681-008-9753-y
Amini, E., Ashraf Mehrabi, A., & Alizadeh, Y. (2019). Effect of gibberellic acid on germination and seedling growth in different sorghum genotypes under salt stress condition. Journal of Seed Research, 9(3), 35–45.
Andrews, J. R., Fryer, M. J., & Baker, N. R. (1995). Characterization of chilling effects on photosynthetic performance of maize crops during early season growth using chlorophyll fluorescence. Journal of Experimental Botany, 46(9), 1195–1203. https://doi.org/10.1093/jxb/46.9.1195
Bewley, J. D., & Black, M. (1982). Physiology and biochemistry of seeds in relation to germination: Volume 2: Viability, dormancy, and environmental control. Springer-Verlag. https://doi.org/10.1007/978-3-642-68643-6
Bewley, J. D., Bradford, K. J., Hilhorst, H. W. M., & Nonogaki, H. (2013). Seeds: Physiology of development, germination and dormancy (3rd ed.). Springer. https://doi.org/10.1007/978-1-4614-4693-4
Bruggink, G. T., Ooms, J. J. J., & van der Toorn, P. (1999). Induction of longevity in primed seeds. Seed Science Research, 9(1), 49–53. https://doi.org/10.1017/S0960258599000057
Cag, S., Cevahir-Oz, G., Sarsag, M., & Goren-Saglam, N. (2009). Effect of salicylic acid on pigment, protein content and peroxidase activity in excised sunflower cotyledons. Pakistan Journal of Botany, 41(5), 2297–2303.
Delavari Parizi, M., Baghizadeh, A., Enteshari, S., & Manouchehri Kalantari, K. (2012). The study of the interactive effects of salicylic acid and salinity stress on induction of oxidative stress and mechanisms of tolerance in Ocimum basilicum L. Iranian Journal of Plant Biology, 4(12), 25–36. [In Persian]
Finch-Savage, W. E., Dent, K. C., & Clark, L. J. (2004). Soak conditions and temperature following sowing influence the response of maize (Zea mays L.) seeds to on-farm priming (pre-sowing seed soak). Field Crops Research, 90(2–3), 361–374. https://doi.org/10.1016/j.fcr.2004.04.006
Ganji Arjenaki, F., Amini Dehaghi, M., & Jabbari, R. (2011). Effects of priming on seed germination of marigold (Calendula officinalis). Advances in Environmental Biology, 5(2), 276–280.
Gilmour, S. J., Hajela, R. K., & Thomashow, M. F. (1988). Cold acclimation in Arabidopsis thaliana. Plant Physiology, 87(3), 745–750. https://doi.org/10.1104/pp.87.3.745
Gupta, T., & Hunsigi, S. L. (2010). Improving the performance of peppermint (Mentha piperita) by physical seed priming under semi-arid conditions. Indian Journal of Medicinal Plant Research, Special Issue, 15–21.
Hay, F. R., & Probert, R. J. (1995). Seed maturity and the effects of different drying conditions on desiccation tolerance and seed longevity in foxglove (Digitalis purpurea L.). Annals of Botany, 76(6), 639–647. https://doi.org/10.1006/anbo.1995.1142
Hayat, S., Ali, B., & Ahmad, A. (2007). Salicylic acid: Biosynthesis, metabolism and physiological role in plants. In S. Hayat & A. Ahmad (Eds.), Salicylic acid: A plant hormone (pp. 1–14). Springer. https://doi.org/10.1007/1-4020-5184-0_1
Heath, R. L., & Packer, L. (1968). Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics, 125(1), 189–198. https://doi.org/10.1016/0003-9861(68)90654-1
Hoseinipoor, A., Yadavi, A. R., Balouchi, H. R., & Moradi, A. (2020). The effect of water potential and deterioration on some indices of germination and biochemical of linseed (Linum usitatissimum var. Norman) seed at different temperatures. Iranian Journal of Seed Science and Technology, 9(2), 19–33. https://doi.org/10.22034/ijsst.2019.123144.1225 [In Persian]
Hussain, S., Khan, F., Hussain, H. A., & Nie, L. (2016). Physiological and biochemical mechanisms of seed priming-induced chilling tolerance in rice cultivars. Frontiers in Plant Science, 7, 116. https://doi.org/10.3389/fpls.2016.00116
Ikić, I., Maričević, M., Tomasović, S., Gunjača, J., Šatović, Z., & Šarčević, H. (2012). The effect of germination temperature on seed dormancy in Croatian-grown winter wheats. Euphytica, 188(1), 25–34. https://doi.org/10.1007/s10681-012-0735-8
Irigoyen, J. J., Emerich, D. W., & Sánchez-Díaz, M. (1992). Water stress induced changes in concentrations of proline and total soluble sugars in nodulated alfalfa (Medicago sativa) plants. Physiologia Plantarum, 84(1), 55–60. https://doi.org/10.1111/j.1399-3054.1992.tb08764.x
Jiang, M., & Zhang, J. (2001). Effect of abscisic acid on active oxygen species, antioxidative defence system and oxidative damage in leaves of maize seedlings. Plant and Cell Physiology, 42(11), 1265–1273. https://doi.org/10.1093/pcp/pce162
Karami, R., Ebrahimi, F., Balouchi, H., & Babaie Zarch, M. J. (2020). Improving germination behavior and seedling characteristics of Chenopodium quinoa Willd. under the influence of salicylic acid and salinity stress. Journal of Seed Research, 10(1), 53–66. [In Persian]
Khan, F., Hussain, S., Khan, S., & Geng, M. (2020). Seed priming improved antioxidant defense system and alleviated Ni-induced adversities in rice seedlings under N, P, or K deprivation. Frontiers in Plant Science, 11, 565647. https://doi.org/10.3389/fpls.2020.565647
Lolaei, A., Khalili, A., Mohammadi, H., & Eslami, S. (2022). Effect of seed priming with salicylic acid on germination and growth indices of borage under salinity stress. Journal of Seed Research, 11(4), 1–14.
Loreto, F., & Velikova, V. (2001). Isoprene produced by leaves protects the photosynthetic apparatus against ozone damage, quenches ozone products, and reduces lipid peroxidation of cellular membranes. Plant Physiology, 127(4), 1781–1787. https://doi.org/10.1104/pp.010497
Mahdavi, B., Modarres Sanavy, S. A. M., Aghaalikhani, M., Sharifi, M., & Dolatabadian, A. (2011). Chitosan improves osmotic potential tolerance in safflower (Carthamus tinctorius L.) seedlings. Journal of Crop Improvement, 25(6), 728–741. https://doi.org/10.1080/15427528.2011.606354
Miller, T., & Chapman, S. J. (1978). Germination responses of three forage grasses to different concentrations of six salts. Journal of Range Management, 31(2), 123–124. https://doi.org/10.2307/3897659
Mousavi Naserabad, M., Moradi, A., Masoumi Asl, A., & Balouchi, H. (2020). Effect of gibberellic acid, germination temperature and stratification on dormancy breaking and seed germination of Smyrnium cordifolium. Iranian Journal of Crop Sciences, 51(2), 199–212. https://doi.org/10.22059/ijfcs.2019.275448.654580 [In Persian]
Najafi, F., Bannayan, M., Tabrizi, L., & Rastgoo, M. (2006). Seed germination and dormancy breaking techniques for Ferula gummosa and Teucrium polium. Journal of Arid Environments, 64(3), 542–547. https://doi.org/10.1016/j.jaridenv.2005.06.009
Paquin, R., & Lechasseur, P. (1979). Observations sur une méthode de dosage de la proline libre dans les extraits de plantes. Canadian Journal of Botany, 57(18), 1851–1854. https://doi.org/10.1139/b79-233
Patade, V. Y., Maya, K., & Zakwan, A. (2011). Seed priming mediated germination improvement and tolerance to subsequent exposure to cold and salt stress in capsicum. Research Journal of Seed Science, 4(3), 125–136. https://doi.org/10.3923/rjss.2011.125.136
Powell, A. A., Yule, L. J., Jing, H. C., Groot, S. P. C., Bino, R. J., & Pritchard, H. W. (2000). The influence of aerated hydration seed treatment on seed longevity as assessed by the viability equations. Journal of Experimental Botany, 51(353), 2031–2043. https://doi.org/10.1093/jexbot/51.353.2031
Purvis, A. C., & Shewfelt, R. L. (1993). Does the alternative pathway ameliorate chilling injury in sensitive plant tissues? Physiologia Plantarum, 88(4), 712–718. https://doi.org/10.1111/j.1399-3054.1993.tb01393.x
Rajasekaran, L. R., Stiles, A., & Caldwell, C. D. (2002). Stand establishment in processing carrots: Effects of various temperature regimes on germination and the role of salicylates in promoting germination at low temperatures. Canadian Journal of Plant Science, 82(2), 443–450. https://doi.org/10.4141/P01-016
Ramezani, A., Haddad, R., & Sedaghati, B. (2016). Effect of sodium hypochlorite concentration and time on disinfection and germination of Catharanthus roseus seeds in vitro conditions. In Proceedings of the 6th National Conference on Sustainable Agriculture and Natural Resources. https://civilica.com/doc/573557
Raziei, Z., Kahrizi, D., & Rostami-Ahmadvandi, H. (2018). Effects of climate on fatty acid profile in Camelina sativa. Cellular and Molecular Biology, 64(5), 91–96. https://doi.org/10.14715/cmb/2018.64.5.15
Russo, R., & Reggiani, R. (2012). Antinutritive compounds in twelve Camelina sativa genotypes. American Journal of Plant Sciences, 3(10), 1408–1412. https://doi.org/10.4236/ajps.2012.310170
Sedghi, M., Nemati, A., & Esmaielpour, B. (2010). Effect of seed priming on germination and seedling growth of two medicinal plants under salinity. Emirates Journal of Food and Agriculture, 22(2), 130–139. https://doi.org/10.9755/ejfa.v22i2.4900
Siadat, S. A., Moosavi, A., & Sharafi-Zadeh, M. (2012). Effects of seed priming on antioxidant activity and germination characteristics of maize seeds under different ageing treatments. Research Journal of Seed Science, 5(2), 51–62. https://doi.org/10.3923/rjss.2012.51.62
Turley, L. M. (2014). The growth, development, and agronomic management of Camelina sativa [Bachelor's thesis, Lincoln University].
Verma, S. K., Bajpai, G. C., Tewari, S. K., & Singh, J. (2005). Seedling index and yield as influenced by seed size in pigeonpea. Legume Research, 28(2), 143–145.
Yadeghari, L. Z., Heidari, R., & Carapetian, J. (2007). The influence of cold acclimation on proline, malondialdehyde (MDA), total protein and pigments contents in soybean (Glycine max) seedlings. Journal of Biological Sciences, 7(8), 1436–1441. https://doi.org/10.3923/jbs.2007.1436.1441
Yousefi, F., Siahpoush, A., Bakhshandeh, A., & Mousavi, S. A. (2021). The effect of hormone seed priming using gibberellic acid on seed germination characteristics and seedling growth of coneflower (Echinacea purpurea). Iranian Journal of Seed Research, 8(1), 173–188. https://doi.org/10.29252/yujs.8.1.173 [In Persian]