Ahmadreza Najafi; Asghar Estaji; Mahdi Ghasemi
Abstract
The percentage and rate of seed germination are critical factors leading to high yield in agriculture, but it has a diminishing effect on environmental stress. Seed pretreatment is one of the ways to increase the strength and velocity of seed germination to deal with salt stress. The purpose of this ...
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The percentage and rate of seed germination are critical factors leading to high yield in agriculture, but it has a diminishing effect on environmental stress. Seed pretreatment is one of the ways to increase the strength and velocity of seed germination to deal with salt stress. The purpose of this study was to investigate the effect of seed pretreatment by peppermint (0.5, 1, and 2%) and seaweed extract (0.5, 1, and 2%) with control on the growth and biochemical traits of Thymus daenensis seedling under salinity stress (0, 50, 100, and 150 mM NaCl) conditions. This experiment was conducted as a factorial as a completely randomized design in four replications at the seedling growth stage. The results showed that seed pretreatment with peppermint and seaweed extract increased the percentage, velocity, and time of germination as well as the number of germinated seeds, in the absence of salinity stress. In seedling growth characteristics, although they were better in the absence of salinity stress, the pretreatment of the seeds significantly reduced the effects of salinity stress compared to the control conditions. The highest amount of chlorophyll in this condition was obtained in seeds treated with 2% seaweed extract. The highest activity of catalase enzyme and polyphenol oxidase enzyme was obtained in 100 and 150 mM salinity stress conditions and seeds treated with 1 and 2% seaweed extract, respectively. Salinity stress had caused a decrease in growth parameters, but seaweed extract pretreatment had improved the conditions.
Nasrin Teimoori; Mokhtar Ghobadi; Danial Kahrizi
Abstract
Drought is one of the most important stresses in reducing plant growth, especially in the seed germination stage. Oxidative stress is one of the consequences of drought in plants. To investigate the reduction of oxidative stress damage caused by drought stress by using silicon in the initial stages of ...
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Drought is one of the most important stresses in reducing plant growth, especially in the seed germination stage. Oxidative stress is one of the consequences of drought in plants. To investigate the reduction of oxidative stress damage caused by drought stress by using silicon in the initial stages of camellia seedling growth, a factorial experiment as completely randomized design was carried out in the seed laboratory of Razi University with three replications. The factors include two camelina genotypes, four levels of drought stress (0, -3, -6, -9 bar using PEG-6000) and five levels of silicon (0, 2, 4, 6 and 8 mM). According to the results, drought stress increased the activity of antioxidant enzymes including peroxidase, catalase and superoxide dismutase and the amount of malondialdehyde, but it decreased the growth characteristics of seedlings and the amount of soluble proteins. The consumption of silicon (especially by 6 and 8 mM concentrations) increased the activity of antioxidant enzymes and seedling growth characteristics. So, consumption of 8 mM silicon increased peroxidase activity by 23.2%, catalase activity by 13.4%, superoxide dismutase activity by 41%, malondialdehyde by 19.1% and protein content. Soluble was 10.8%. Therefore, the consumption of silicon 8mM by activating the antioxidant system moderated the effects of oxidative stress caused by drought stress and increased the growth characteristics of camellia seedlings.
Masoumeh Asadi Aghbolaghi; Farzaneh Razavi
Abstract
This study was performed to investigate the effect of pumpkin (Cucurbita pepo L.) seed’s pre-treatment by salicylic acid and ascorbic acid on different aspects of seed germination and biochemical features. A factorial experiment was conducted based on a completely randomized design with three replications. ...
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This study was performed to investigate the effect of pumpkin (Cucurbita pepo L.) seed’s pre-treatment by salicylic acid and ascorbic acid on different aspects of seed germination and biochemical features. A factorial experiment was conducted based on a completely randomized design with three replications. Experimental treatments included two levels of seed maturity (normal seeds and aged seeds) and five levels of priming (control, ascorbic acid 50 and 100 mg/l, and salicylic acid 50 and 100 mg/l). The seed traits were finally measured included the germination percentage, first and second seed vigor index, seedling dry weight, peroxidase and superoxide dismutase activity, electrolyte leakage, soluble protein, and soluble carbohydrate. Based on the results, the seed pre-treatment with 100 mg/l ascorbic acid and salicylic acid could perform the highest germination indices. Moreover, the germination percentage (74%) was improved in aged seeds pre-treated by ascorbic acid and salicylic acid (especially at a concentration of 100 mg/l). Also, the antioxidant enzyme activity was increased while the cell electrolyte leakage was significantly decreased. In total, results indicate, the pre-treatment with salicylic acid and ascorbic acid, not only improved the germination quantity and vigor but also improved the germination quality mainly by the development of carbohydrate and protein content in pumpkin seeds.
Afshin Mozafari; Sajad Fathollahy
Abstract
In order to Investigation the Effect of Seed Biopriming with Plant Growth Promoting Rhizobacteria (PGPR) on antioxidant enzymes activity of seedling and Germination Indices of Two Wheat Cultivar under Salt Stress Conditions, an experiment was conducted as a factorial experiment based in a completely ...
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In order to Investigation the Effect of Seed Biopriming with Plant Growth Promoting Rhizobacteria (PGPR) on antioxidant enzymes activity of seedling and Germination Indices of Two Wheat Cultivar under Salt Stress Conditions, an experiment was conducted as a factorial experiment based in a completely randomized design with three replications in a laboratory of Islamic Azad University branch of Ilam, in 2017. Experimental factors included salt stress at four levels: zero (Control), 50, 100 and 200 mM sodium chloride, PGPR bacteria in two levels: non-inoculation with PGPR (control) and seed inoculation with PGPR and wheat cultivars include: Tajan and Ofogh. The genus and species of PGPR bacteria used in this study included Azotobacter chroococcum, Azospirillum berasilense and Pseudomonas potida, which were obtained from the Iranian soil and water research institute. Experimental traits included Activity of superoxide dismutase enzymes (SOD), catalase (CAT) and glutathione peroxidase (GPX), dry weight of radicl, gomul and seedling, germination percentage, germination rate and seed vigor index. The main effect of experimental factors on all traits was highly significant (P≤0.01). The results showed that Ofogh was superior to Tajan cultivar for all germination indices, dry weight of radicl, gomul and seedling, Activity of antioxidant enzymes. With increasing salt stress, all of traits were decreasing. Priming seeds with PGPR bacteria increased the traits studied. In general, priming of wheat seeds with PGPR in comparison with control treatment (non-inoculation) by improving germination indices and Activity of antioxidant enzymes to some extent reduced the harmful effects of salinity stress.
Roksana Nazari; soheil parsa; Reza Tavakkol Afshari; sohrab mahmodi
Abstract
In order to investigate the effect of priming on the improvement of physiological indices of antioxidant enzymes in soybean seeds, a factorial experiment was conducted in a completely randomized design with three replications at the Seed Science and Technology Laboratory of Tehran University in 2017. ...
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In order to investigate the effect of priming on the improvement of physiological indices of antioxidant enzymes in soybean seeds, a factorial experiment was conducted in a completely randomized design with three replications at the Seed Science and Technology Laboratory of Tehran University in 2017. The factors studied included two levels of deterioration (48 and 72 hours), three levels of salicylic acid (0, 300, 600 μM) and three times the salicylic acid application (before decay, post-fall, before and after deterioration). The traits measured included antioxidant enzymes (glutathione redactase, superoxide dismutase, catalase, peroxidase, ascorbate peroxidase, glutathione peroxidase, membrane peroxidation or malondialdehyde). The results showed that deterioration level increasing, decrease the antioxidant enzymes activity and increase membrane peroxidation and soybean seed priming with salicylic acid hormone restore the decayed seeds and increase the activity of antioxidant enzymes. The highest activity of ascorbate peroxidase activity was observed at 72 hours with the time of salicylic acid application before and after deterioration at a concentration of 300 μm and 6.98. Reduction of malondialdehyde leakage rate when combined with 300 μM concentration of salicylic acid Was observed at 1.45. The results also showed that the priming at the same time improves the chemical properties of the seeds in the deterioration.
Parisa Sheikhzadeh; Hamid Shafiyar; Saeed Khomari; Hamid Reza Mohammad Doost
Abstract
Borage is one of the important medicinal plants with unequal and poor seed germination as a result of deterioration during storage. With attention to abundance of salty water and soils in country, this research was contacted to study of the effect of priming and deterioration of seed on germination, ...
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Borage is one of the important medicinal plants with unequal and poor seed germination as a result of deterioration during storage. With attention to abundance of salty water and soils in country, this research was contacted to study of the effect of priming and deterioration of seed on germination, growth and biochemistry characteristic of borage under salinity stress. The experimental treatments were salinity stress, seed priming and deterioration duration (0, 8 and 14 hours). The results showed that percentage and rate of germination, seedling dry weight and length and weight were decreased with increasing of salinity stress levels and duration of deterioration. Seedlings dry weight loss was about 75% to 78% under salinity stress. Activity of peroxidase and catalase enzymes and proline content in seedling were increased under salinity stress, and this increase in seedlings from primed seeds was significantly higher than those of control seeds. Seed priming significantly increased the seed germination, growth and prolin content of seedling in both salinity stress and deterioration conditions. Seed priming caused a 1.6 and 2.5- fold increase in the catalase activity of seedlings derived from undeteriorated and deteriorated (14 hours) seeds, respectively. Generally, seed priming with ascorbic acid was reduced the negative effects of salinity stress and deterioration through increasing seed vigor and improving the biochemical properties of seedlings, and led to improve the seed germination and seedling growth under favorable and unfavorable conditions.
Ahmad Afkari
Abstract
In order to investigate the effects of seed inoculation with arbuscular mycorrhizal fungi on some chemical characteristics and seed germination of corn (Zea mays L.) under drought stress conditoins, a factorial experiment based on randomized complete design with three replications was conducted at the ...
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In order to investigate the effects of seed inoculation with arbuscular mycorrhizal fungi on some chemical characteristics and seed germination of corn (Zea mays L.) under drought stress conditoins, a factorial experiment based on randomized complete design with three replications was conducted at the physiology laboratory of Islamic Azad University Branch Ardabili, during 2015. Studied factors were: drought stress in four levels (Zero, -4, -8 and -12 bar) and seed inoculation with mycorrhizal fungus in four levels (no inoculation, seed inoculation with Funneliformis mosseae, Rhizoglomus fasciculatum and Claroideoglomu etanicatum. The results showed that the effect of drought stress and seeds inoculation with mycorrhizal fungi on germination components, seed protein content and antioxidant enzymes was significant at 1% probability Level. Seed inoculation with Rhizoglomus fasciculatum showed higher antioxidant enzymes activity and higher seed protein content compared with inoculation with Funneliformis mosseae, Claroideoglomus etanicatum and no inoculation. In drought stress conditions, plants will reduce oxidative stress leads to increased activity of antioxidant enzymes has been. The results of the mean comparison of data showed that with increasing drought stress germigation percen and germination rate index decreased and the means of germination time, increased. With respect to the results of this study, it seems that mycorrhiza fangi by an improvement in water and nutrient absorption, improve plant growth in drought stress condition. Thus, in order to increasing of germination components and chemical characteristics, it can be suggested that seeds inoculation of corn was applied with Rhizoglomus fasciculatum
narges khamadi; majid nabipor; habibolah roshanfeker; afrasyab rahnama
Abstract
One of factors in successful seed hydropriming technique is seed imbibition duration. To evaluate hydroprimed seed vigor of three wheat cultivars as affected by seed imbibition durations and also evaluation biochemical changes during hydropriming, two experiments was done as factorial arrangement with ...
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One of factors in successful seed hydropriming technique is seed imbibition duration. To evaluate hydroprimed seed vigor of three wheat cultivars as affected by seed imbibition durations and also evaluation biochemical changes during hydropriming, two experiments was done as factorial arrangement with completely randomized design. In first experiment, effect of 7 hydropriming durations (0 , 6 , 8 , 10 , 12 , 14 and 16 h ) was research on germination and seedling growth of Star, Chamran and Fong cultivars. In second experiment, some biochemical traits and enzyme activities was measured in primed seeds for 6 , 8 and 10 h hydropriming durations. Results of first experiment showed that hydropriming with 8 h imbibition has the most positive effect on seed vigor of wheat cultivars. Among of cultivars, Star has the higher root length and root weight. Star and Chamran cultivars have no significant difference for germination rate and the lowest of this trait was observed in fong cultivar. Results of second experiment showed that longer hydropriming duration caused greater α amylase enzyme activity and higher contents of soluble carbohydrates and proteins, also increase content of malondialdehyde and insufficient antioxidant enzyme activities in 10 h than 8 h hydropriming duration was observed. Among cultivars the highest and lowest α amylase enzyme activity and soluble carbohydrates and proteins was observed in Star and Fong cultivars respectively. In this research correlation antioxidant enzyme activities with germination and seedling growth was positive and significant.
S.R. Khatami; M. Sedghi; R. Seyed Sharifi
Abstract
In this research effect of priming types was investigated on the activity of antioxidant enzymes including Catalase (CAT), Ascorbate peroxidase (APX), Superoxide dismutase (SOD), Malone dialdehyde (MDA) and Gluthation reductase (Gred) in hybrid maize single cross 704 under drought stress. Treatments ...
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In this research effect of priming types was investigated on the activity of antioxidant enzymes including Catalase (CAT), Ascorbate peroxidase (APX), Superoxide dismutase (SOD), Malone dialdehyde (MDA) and Gluthation reductase (Gred) in hybrid maize single cross 704 under drought stress. Treatments were drought at 4 levels (0, -3, -6 and -9 bar) and priming (Hormone, hydro, osmo, ascorbic and control). Germination percentage, seedling vigor and the activity of antioxidant enzymes were measured. Results showed that interaction of drought and priming was significant an all traits except of germination percentage. The highest seedling vigor (15.996) obtained from control condition. The highest CAT activity (35 μmol min-1 mg-1) observed in ascorbic acid priming at -9 bar drought stress. Also, the highest APX activity was related to hormone priming and -9 bar. While the lowest activity observed in control drought and osmo-priming. The highest amount of MDA achieved in -9 bar and control priming. The highest SOD activity observed in -9 bar. The highest Gred activity obtained by ascorbic acid priming at -9 bar.
