Effects of Seed Inoculation with Free Living Nitrogen Fixing Bacteria and Time of Nitrogen Foliar Application on Contribution of Stem Reserves in Grain Yield, Rate and Effective Grain Filling Period of Triticale

Document Type : Original Article

Authors

Abstract

In order to investigate the effects of free living nitrogen fixing bacteria application and time of nitrogen spraying on contribution of stem reserves in grain yield, rate and effective grain filling period of Triticale, a factorial experiment was conducted based on a randomized complete block design with three replications in Research farm of the Faculty of Agriculture, University of Mohaghegh Ardabili in 2012. Treatments consisted of different nitrogen spraying times in four levels (no spraying as control, spraying in boot stage, ear emergence, grain filling period) and seed inoculation with plant growth promoting rhizobacteria in four levels containing (without inoculation as control, seed inoculation with Azotobacter chroococcum strain 5, Azospirillum lipoferum strain OF, Psedomunas putida strain 9). Results indicated that spraying time of nitrogen fertilizer and seed inoculation with free living nitrogen fixing bacteria had significant effects on yield, yield components, rate and effective grain filling period of Triticale. Maximum of grain weight (0/054 gr) and effective grain filling period (34.17 days) were obtained in seed inoculation with Azotobacter× nitrogen spraying in boot stage. Maximum of contribution of stem reserves in grain yield (30.63 %) was obtained in no nitrogen spraying × no seed inoculation with PGPR and minimum of  it (8.12 and 8.13 %) were obtained in nitrogen spraying in boot stage × seed inoculation with Azotobacter and spraying in ear emergence × seed inoculation with Azotobacter. It seems that in order to increase the grain yield,rate and effective grain filling period of Triticale, it can be suggested that nitrogen spraying is applied in boot stage and seed inoculation with Azotobacter

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