Study of Fungal Populations in Winter Type Canola Seeds

Document Type : Original Article

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Abstract

The contamination of canola seeds to various fungal agents is important like other crops and it is varied annually. Diseases like blackleg and sclerotinia stem rot are considered as the most important diseases in Iran. The possibility of their direct spread through seed contaminations and indirect spread through infected debris in seed masses will have danger of their development. For investigating canola seed contamination to fungal agents, seed samples were collected from seed parties of canola grown in cold areas including Alborz, Kermanshah and Markazi provinces. The surface of seeds were observed to differentiate and separate different abnormal appearances including wrinkle, presence of cracks, oozes, hyphal shape particles, and black spots. The presence of sclerotia of stem rots disease causal agent, and plant debris containing pycnidia of blackleg disease were checked in seed samples using stereo microscope. The identification of fungi on the seeds was done with and without surface disinfection processes. The plant debris in non-sieved seed samples did not have structures similar to pycnidia of blackleg disease. The results obtained demonstrated that the seed samples were contaminated to fungi including  Penicillium sp.,  Alternaria sp.,  Aspergillus sp., and  Rhizopus sp.. 

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References

Afshari Azad, H. 2001. The major diseases of canola. AREO Publ.99 p. (In Persian).
Afshari Azad, H., S.R. Dalili, M. Salati and M.A. Amini Khalaf. 2008. Distribution of canola blackleg disease in Iran. Proc. 18th Iranian. Plant Protec. Congr., Hamedan, Iran. 199(Abst.).
Aghajani, M.A. 2008. Sclerotinia stem rot of Canola. Agricultural and Natural Resources Research Center of Golestan. Tech. J. 44, 36 p. (In Persian).
Alizadeh, B., H. Amiri Oghan, A.H. Shirani Rad and S. Rahmanpour. 2015. Canola Ann. Hand. SPII, Karaj, Iran. 43p. (In Persian).
Aneja J.K. and A. Agnihotri. 2013. Alternaria blight of oilseed brassicas: epidemiology and disease control strategies with special reference to use of biotechnological approaches for attaining host resistance. J. Oil. Brass. 2013 4(1): 1-10.
Bansal V K, S.G. Seguin, G.F.W. Rakow and G.A. Petric. 1990. Reaction of Brassica species to infection by Alternaria brassicae. Can. J. Plant Sci. 70: 1159-1162.
Barnett, H.L. and B.B. Hunter. 1972. Illustrated Genera of Imperfect Fungi. Burgess Pub. Co., Minneapolis, Minnesota. 241pp.
Basirnia, T. 2011. Study on canola seed mycoflora in Fars province. Plant Dis. Res.1: 47-56. (In Persian with English abstract)
Boland, G.J.  and R. Hall. 1994. Index of plant hosts of Sclerotinia sclerotiorum. Can. J. Plant Pathol. 16:93-108.
Bradley, C.A., R.A. Henson, P.M. Porter, D.G. LeGare, L.E. del Río, and S.D.  Khot. 2006. Response of canola cultivars to Sclerotinia sclerotiorum in controlled and field environments. Plant Dis. 90:215-219.
Copeland, L.O. and M.B. McDonald. 2014. Principles of Seed Science and Technology. (Trans. F. Akram Ghaderi, B. Kamkar and A. Soltani). JDN Press. Mashad Univ., Mashad, Iran. (In Persian).
ISTA. 1996. Seed Sci. and Technol.21(Suppl.): 1B288.
Kaczmarek, J. and M. Jędryczka. 2011. Characterization of two coexisting pathogen populations of Leptosphaeria spp., the cause of stem canker of brassicas. Acta Agrobot. 64(2): 3-14.
Kharbanda P.D, B.D.L. Fitt, R.M. Lange, J.S. West, A.H. Lamey and D.V. Phillips. 2001. Common names of plant diseases: Diseases of rapeseed = Canola (B. napus L. and Brassica rapa L. (= B. campestris L.)) [Online]Available at
 http://www.apsnet.org/online/common/names/rapeseed.asp.
Kimber, D.S. and D.I. McGreogor. 2004. Oilseed rape: Physiology, Agronomy, Breeding, Biotechnology. (Trans. M. Azizi, A. Soltani and S. Khavari Khorasani). JDN Press. Mashad Univ., Mashad, Iran. (In Persian).
Lamey, H. A. 1995. Survey of blackleg and sclerotinia stem rot of canola in North Dakota in 1991 and 1993.Plant Dis.79(3): 322-324.
Montemuro, N., and A. Visconti. 1992. Alternaria metabolites chemical and biological data. P. 449-557. In Y. Chelkowski and A. Visconti (eds). Alternaria: Biology, Plant Disease and Metabolites. Amsterdam: Elsevier.
Morral, R.A.A., J. Dueck and P.R. Verma. 1984. Yield losses due to Sclerotinia stem rot in western Canadian rapeseed. (Abstr.) Can. J. Plant Pathol. 6:265.
Nepal, A., S. Markell, J. Knodel, C.A. Bradley and L.E. del Río Mendoza. 2014. Prevalence of blackleg and pathogenicity groups of Leptosphaeria maculans in North Dakota. Plant Dis. 98:328-335.
Pasban Eslam, B. 2011. Seed Science and Technology. Parivar Press. Tabriz, Iran (In Persian).
Rahmanpour, S. 2003. Evaluation of resistance of canola lines and cultivars to stem rot caused by Sclrotinia sclrotiorum under controlled condition. Final report of research project. SPII. (In Persian).
Rahmanpour, S. 2008. Studies on the role of the glucosinolate-myrosinase system in resistance of oilseed rape to Sclerotinia sclerotiorum. Ph.D. Thesis. Univ. of New England, Armidale, NSW, Australia.
Rahmanpour, S. and H. Amiri-Oghan. 2008. Identification and management of Canola diseases. Agric. Educ. Publ.182 p. Karaj, Iran (In Persian).
Silvana D.M. and L.M.V. Skares,  2000.  A method for the determination of two Alternaria toxin, Alternariol and Alternariol monomethyl ether, in tomato products. Braz. J. Microbiol. 31: 315-320.
Szopinska, D., K.  Tylkowska and A. Stach, 2007. Relationship between seed development stage, germination, occurrence and location of fungi Oilseed Rap (Brassica napusspp. Oleiferal.) seeds and the presence of Alternaria and Cladosporium spp. Spores in the air. Elec. J. Polish Agric. Univ. 10: 4-19.
Vinas, I., J. Palma, S. Garza and A. Visconti. 1994. Natural occurrence of aflatoxin and Alternaria mycotoxin in oilseed rape from Catalonia (Spain)- incidence of toxigenic strain. Mycopathol. 128:175-179.
Williams, P.H., 1980. Blackleg, a fellow traveler. Plant Dis 64(8): 736-742. 
Zhang, X.,  R.P. White, E. Demir, M. Jedryczka, R.M. Lange, M. Islam, Z.Q. Li, Y.J. Huang, A. M. Hall, G. Zhou, Z. Wang, X. Cai, P. Skelsey and B.D.L. Fitt. 2014. Leptosphaeria spp., phoma stem canker and potential spread of L. maculans on oilseed rape crops in China. Plant Pathol. 63:598-612.

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