Seed germination responses of sorghum lines and cultivars under salinity stress conditions

Document Type : Original Article

Authors

Abstract

   To evaluate the effect of salinity stress on germination indices of sorghum, this experiment was conducted using a factorial arrangement based on a completely randomized design (CRD) with three replications at Cereal laboratory of College of Agriculture, Shiraz University, Shiraz, Iran in 2015. Treatments included salinity levels (Control (0.5), 3.5, 6.5, 9.5 and 12.5 dS.m-1) and 48 sorghum lines and cultivars.Analysis of variance showed that the effect of salinity was significant on all measured traits at 1% level. In this experiment, increased salinity caused a significant reduction in germination percentage, germination rate, seedling dry weight and vigor index. The highest germination percentage (86%) as well as seedling length (386.2 mm), and vigor index (332.33) was found in KDFGS19 line. Ghalami Herat cultivar had the highest germination rate (12.6/day). Orthogonal comparison for two groups of genotypes indicated that there were significant differences between cultivars and lines for all measured traits (except for seedling dry weight). Lines had higher germination percentage and germination rate compared to cultivars, however, for seedling length and vigor index, cultivars had higher levels than lines. Lines had higher germination percentage and germination rate compared to cultivars, however, for seedling length and vigor index, cultivars had higher level than lines.

Keywords


 
 
Abnous, M. 2001. Evaluation of physiological effect of drought stress on seed germination and seedling growth of lentil. MS.c. Thesis, Ferdowsi Univ. of Mashhad.
Ahrar, A. R., S.A. Tabatabaei and A. Foman-ajirloo A. 2010. Evaluation of grain sorghum cultivars for salinity tolerance in various growth stages. M.Sc. Thesis. Islamic Azad University, Maybod Branch, Yazd, Iran.
Ali, S. A. M., A. Y. Idris  and M. S. A. Abo. 2014. Effect of Salinity on Seed Germination and Seedling Growth of Pearl millet (Pennisetum glaucum L.) and Sorghum (Sorghum bicolor L.). J. Plant. Pest Sci. 1(1): 1-8.
Alizadeh,  M. A. 1997. Loss of vigor and disease resistance in wheat seeds stored in Iranian climate. Ph. D. Thesis. Salford: Univ. of Salford, 289 p.
Almodares, A., M. R. Hadi and B. Dosti. 2007. Effects of salt on germination percentage and seedling growth in sweet sorghum cultivars. J.  Biol. Sci. 7(8): 1492-1495.
Anagholi, A., S.A. Tabatabaei and A. Foman. 2010. Evaluation of salinity tolerance of forage sorghum varieties with stress tolerance and susceptibility indices. (In Persian with English Abstract) Electronic J. Crop Prod. 3(1): 89-102.
Ashraf, M., and M. R. Foolad. 2007. Role of glycine betaine and proline in improving plant abiotic stress resistance. Environ. Exp. Bot. 59: 206-216.
Azadi, M. S., E. Yunesi, S.A. Tabatabaei and O. Ansari. 2013. Effect of Ascorbic acid on germination traits and biochemical variations of Sorghum under salinity stress. (In Persian, with English Abstract). J. Seed Sci. 3(3): 43-51.
Blum, A., J. Mayer, G. Golan and B. Sinmena. 1999. Drought resistance of DH lines population of rice in the field. In: Ito, J.O. Tool, B. Hardy (ed) Genetic improvement of rice for water-limited environments International Rice Research Institute, Los Bannos, Mallina, Philippines. pp: 319-330.
Bradford, K. J. 1986. Manipulation of seed water relations via osmotic priming to improve germination under stress conditions. Hortic. Sci, 21: 1105-1112.
Cramer, G. R., K. Urano, S. Delrot, M. Pezzotti and K. Shinozaki. 2011. Effects of abiotic stress on plants: a systems biology perspective. BMC Plant Biol, 11(1): 163.
Demir Kaya, M., G. Okcu, M. Atak, Y. Cikili  and O. Kolsarici. 2006. Seed treatments to overcome salt and drought stress during germination in sunflower (Helianthus annuus L.). Eur. J. Agron. 24: 291-295.
Dianati-Tilaki, G., B. Shakarami, M. Tabari and B. Behtari. 2011. The effect of NaCl priming on germination and early growth of seeds of Festuca ovina L. under salinity stress conditions. (In Persian, with English Abstract) Iranian J. Range Desert Res. 18(3): 452-462.
El Naim, A. M., K.E. Mohammed, E.A. Ibrahim and N.N. Suleiman. 2012. Impact of salinity on seed germination and early seedling growth of three sorghum (Sorghum bicolor L. Moench) cultivars. Sci. Technol. 2(2): 16-20.
Emam, Y. 2013. Cereal Crop Production (1st edition). Iran Univ. Press. 190p.
Endalew, T., A. Mebeaselassie and T. Kindie. 2013. Genotypic variation for salinity tolerance in sorghum (Sorghum bicolor (L.) Moench) genotypes at early growth stages. J. Stress Physiol. Biochem. 9(2): 253-262.
Esmaelipour, N., and M. Mojadam. 2009.Effect of seed hydro priming on germination and seedling growth of sweet sorghum under salinity stress. (In Persian, with English Abstract) J. Crop Physiol, 1(3):51-59.
Gholinezhad, E. 2011. Effect of salinity stress on germination traits of wheat cultivars. (In Persian, with English Abstract) J. Seed Sci .Technol.1(1): 14-21.
Houle, G., L. Morel,  C.E. Reynolds and J. Siegel. 2001. The effect of salinity on different developmental stages of an endemic annual plant, Aster laurentianus (Asteraceae). Am. J. Bot. 88: 62-67.
Igartua, E., M.P. Gracia and J.M. Lasa. 1994. Characterization and genetic control of germination, emergence responses of grain sorghum to salinity. Euphytica. 76(3):185–193.
Katergi, N., J.W. Van Hoorn, A. Hamdy, F. Karam and M. Mastrortilli. 1994. Effect of salinity on emergence and on water stress early seedling growth of sunflower and maize. Agric. Water Manage. 26: 81-91.
Koca, M., M. Bor, F. Ozdemir and I. Turkan. 2007. The effect of salt stress on lipid peroxidation, antioxidative enzymes and proline content of sesame cultivars. Environ. Exp. Bot. 60: 344-351.
Maas, E.V., J.A. Poss and G.J. Hoffamn. 1986. Salinity sensitivity of sorghum at three growth stages. Irrig. Sci. 7: 1–11.
Macharia, J.M., J. Kamau, J.N. Gituanja and E.W. Matu. 1994. Effects of sodium salinity on seed germination and seedling root and shoot extension of four sorghum [Sorghum bicolor (L.) Moench] cultivars. Inter Sorghum and Millets Newsl. 35:124–125.
Maguire, J. D. 1962. Speed of germination—aid in selection and evaluation for seedling emergence and vigor. Crop Sci. 2: 176-177.
Patane C., A. Saita and O. Sortino. 2013. Comparative effects of salt and water stress on seed germination and early embryo growth in two cultivars of sweet sorghum. J. Agron. Crop Sci. 199(1), 30-37.
Rani, C. R., C. Reema, S. Alka and P.K. Singh. 2012. Salt tolerance of Sorghum bicolor cultivars during germination and seedling growth. Res. J. Recent Sci. 1(3): 1-10.
Ranjbar. G.H., and H. Pirasteh-Anosheh. 2015. A glance to salinity researches in Iran; Emphasis on improved crop production. (In Persian, with English Abstract)  Iranian J. Cop Sci. 17(2):1-14.
SitiAishah, H., A.R. Saberi, R.A. Halim and A.R. Zaharah. 2010. Salinity effects on germination of forage sorghums. J. Agron. 9 (4): 169-174.
Soltani, A., M. Ghalipoor and E. Zeinali. 2006. Seed reserve utilization and seedling of wheat as affected by drought and salinity. Environ. Exp. Bot. 55: 195-200.