Improvement of sugar beet seed germination characteristics using priming with Multi-Walled Carbon Nano Tubes (MWCNTs)

Document Type : Original Article

Authors

Abstract

In this study, the effect of nanohydropriming using Multi-Walled Carbon NanoTubes (MWCNT) on improving the germination and growth of sugar beet seed, in comparison with hydropriming, was studied. Accordingly, hydropriming with water and nanohydropriming with three concentrations of water-based colloidal MWCNT,s as the carbon nanotube concentrations (0, 10, 20 and 40 ppm), four priming time and two primed seed storage time as treatments in a completely randomized design with three replications were applied in vitro for sugar beet variety Pars. Characters including germination rate, germination percentage, root length, shoot length, fresh weight, dry weight, and vigor were measured. Results showed that differences between treatments for all traits were significant at alpha 1%. Analysis of variance and comparison of hydropriming group against nanohydropriming showed a significantly improved germination rate, germination percentage and seedling vigor of sugar beet variety Pars in nanohydropriming. The mean comparison showed that the nanohydropriming significantly improved traits germination speed, germination percentage and seedling vigor of sugar beet variety in comparison with hydropriming. In addition, the results of data grouping within multi-walled carbon nanotubes showed that the germination traits were significantly improved at concentration of 40 ppm of carbon nanotubes at the 18 hours priming time, and 60 days storage after priming. Accordingly, with respect to the direct and indirect benefits of sugar beet seed priming, treating seeds of Pars variety with carbon nanotubes 40 ppm for 18 hours and 60 days before planting is recommended.

Keywords


Agrawal, S. and P. Rathore, 2014. Nanotechnology Pros and Cons to Agriculture: A review. Int. J. of Curr. Microbiol. and Appl. Sci. 3(3):43-55.
Alavi, Z.S., H. Roushanfekr, P. Hasibi, and M. Mesgarbashi. 2012. Effect of Osmo and Hydro-priming on the rate and percent of germination of sugar beet genotypes under salt stress. Proc. Second Conf. Seed Sci. Technol.- Mashhad (In Persian).
Basra, S.M.A., M. Farooq. K. Hafeez and N. Ahmad, 2004. Osmohardening: a new technique for rice seed invigoration. Int. Rice. Res. Notes, 29: 80-81.
Campbell, L.G., and J.W. Enz. 1991. Temperature effects on sugar beet seedling emergence. J. of Sugar Beet Res. 28:129-140.
Chegini, M.A., and V. Yousef-Abadi, 2006. The effect of processing (priming) on the quality of sugar beet seed. Final report. Sugar Beet Seed Inst. (In Persian).
Durrant, M.J., S.J. Mash, and K.W. Jaggard. 1993. Effects of seed advancement and sowing date on establishment, bolting and yield of sugar beet. J. Agric. Sci. Cambridge. 121: 333-341.
Foti, R., K. Abureni. A. Tigere. J. Gotosa and J. Gerem, 2008. The efficacy of different seed priming osmotica on the establishment of maize (Zea mays L.) caryopses. J. Arid. Environ. 72: 1127-1130.
Govahi, M., M.J. Arvin, and G. Safari. 2007. Incorporation of plant growth regulators into the priming solution improves sugar beet germination, emergence and seedling growth at low-temperature. Pak. J. Biol. Sci., 10(19): 3390-3394.
Haghighi, M., and Z. Afifi Pour. 2011. The effect of priming with carbon nanotubes, nano-titanium and copper nanoparticles on germination characteristics of onion, First National Congress of Science and New Technologies of Agriculture, Zanjan, Zanjan Univ. (In Persian).
Hossini, A., and A. Koochaki. 2007. The effect of different priming treatments on germination rate and percentage of four varieties of sugar beet seed. J. Iranian Agric. Res., No. 9, pp. 69-76 (In Persian).
Husen, A. and K.S. Siddiqi. 2014. Carbon and fullerene nanomaterials in plant system. J. Nanobiotechnol. 12:16.
Jabbari, R., M. Amini Dahaghi, F. Ganji Arjanki, and K. Agahi. 2010. The effects of priming time and method on germination of cumin (Cuminum cyminum L). School. Agric. Sci., Issue 4, pp. 23-30 (In Persian).
Jalilian, A., and R. Tavakol-Afshari, 2004. Osmopriming effect on sugar beet seed germination under drought stress. J. Agric. 27 (2): 23-36 (In Persian).
Ke, P.C., and R. Qiao. 2007. Carbon nanomaterials in biological systems. J. Phys. Condense Matter, 19:373101–373125.
Khodakovskaya, M., E. Dervishi, M. Mahmood, Y. Xu, Z. Li, F. Watanabe, and A.S. Biris. 2009. Carbon Nanotubes Are Able To Penetrate Plant Seed Coat and Dramatically Affect Seed Germination and Plant Growth. ACS Nano, 3 (10), pp 3221–3227.
Kuppusamy, N. and U. Ranganathan. 2014. Storage potential of primed seeds of okra (Abelmoschus esculentus) and beet root (Beta vulgaris). Aust. J. Crop Sci. 8(9) 1290-1297.
Lin, C., B. Fugetsu, Y. Su, F. Watari. 2009. Studies on toxicity of multi-walled carbon nanotubes on Arabidopsis T87 suspension cells. J. Hazard. Mater. 170, 578-583.
Morla, S., C.S.V. Ramachandra, R. Chakrapani.  2011. Factors Affecting Seed Germination and Seedling Growth of Tomato Plants cultured in Vitro Condition. J. Chem. Biol. Phy. Sci. Vol.1, N0.2, Sec. B.328-334.
Murungu, F.S., P. Nyamugafata, C. Chiduza, L.J. Clark, and W.R. Whalley. 2003. Effects of seed priming aggregate size and soil matric potential on emergence of cotton (Gossypium hirsutum L.) and Maize (Zea mays L.). Soil and Tillage Res. 74: 161- 168.
Nair, R., S.H. Varghese, B.G. Nair, T. Maekawa, Y. Yoshida, D.S. Kumar. 2010. Nanoparticulate material delivery to plants. Plant Sci. 179, 154-163.
Phartyal, S.S., R.C. Thapliyal, J.S. Nayal, M.S. Rawat, G. Joshi. 2003. The influences of temperature on seed germination rate in Himalayan elm (Ulmus Wallichiana). Seed Sci. Technol. 31:83-93.
Salehi M., and F. Tamaskony. 2009. Effect of priming on germination and seedling growth of canola compared with nanosilver under salt stress. J. Plant Sci. Res. Number 16, No. 4. pp 57-52 (In Persian).
Sliwinska, E, E. Jendrzejczak. 2002. Sugar-beet seed quality and DNA synthesis in the embryo in relation to hydration–dehydration cycles. Seed Sci. Technol. ;30:597–608.
Srinivasan, C. and R. Saraswathi. 2010. Nano-agriculture – carbon nanotubes enhance tomato seed germination and plant growth. Curr. Sci., vol. 99, No. 3, 10.
Tan, X., B. Fugetsu. 2007. Multi-walled carbon nanotubes interact with cultured rice cells: evidence of a self-defence response. J. Biomed. Nanotechnol. 3, 285-288.
Tan, X., C. Lin, B. Fugetsu. 2009. Studies on toxicity of multi-walled carbon nanotubes on suspension rice cells. Carbon: 47, 3479-3487.
Torre-Roche, R., J. Hawthorne, Y. Deng, B. Xing, W. Cai, L.A. Newman, Q. Wang, X. Ma, H. Hamdi, J.C. White. 2013. Multiwalled carbon nanotubes and C60 fullerenes differentially impact the accumulation of weathered pesticides in four agricultural plants. Environ. Sci. Technol., Volume 47(21), pp 12539-12547.
Xiao-fang, S., Z. Qingsong and L. Youlinag, 2000. Regulation of salt tolerance of cotton plants at seedling emergence stage by soaking seeds in pix (DPC) and CaCl solutions. Jaingsu J. Agric. Sci., 16: 204-207.