Effect of cytokinin on morphological and physiological characteristics and antioxidant enzymes activity of aged groundnut (Arachis hypogaea L.) seeds under drought stress

Document Type : Original Article

Authors

Abstract

In order to investigate the effect of cytokinin on improvement of aged groundnut seeds under drought stress, a factorial experiment was done based on completely randomized design with three replications. Seed priming with different concentrations of cytokinin including 0 (hydropriming), 50, 100 and 150 ppm in drought stress levels (0, -0.4, -0.6 and -0.8 MPa) was performed. Germination percentage, mean germination time, germination rate, seed vigor, seedling length, soluble carbohydrates, soluble proteins, memberane electrical conductivity, malondialdehyde content and antioxidant enzymes activity (catalase, ascorbate peroxidase and superoxide dismutase) were evaluated. The results showed that seed priming with different concentrations of cytokinin alleviated significant decrease of germination and seedling parameters of aged seeds under drought stress. At -0.8 MPa, seed priming with 100 ppm of cytokinin increased germination percentage, vigor index , soluble carbohydrates and proteins 0.65, 1.73, 1.09, 1.05, and in 150 ppm of cytokinin 1.37, 4.51, 2.5, 2.85 fold, as well as the activity of catalase, superoxide dismutase and ascorbate peroxidase increased by 15.92, 13.35, 33.17 and 25.47, 17.96, 45.19% in comparison to control, respectively. Therefore, cytokinin pre-treatment specialy with 150 ppm is effective in germination and seedling indices of aged groundnut seeds by decreasing oxidative stress due to drought stress.

Keywords

References

Andoh, H. and T. Kobata, 2002. Effect of seed hardening on the seedling emergence and alpha amylase activity in the grains of wheat and rice sown in dry soil. Japan. J. Crop Sci. 71: 220–225.
Azadi, M.S., S.A. Tabatabaei, E. Younesi, M.R. Rostami, and M. Mombeini, 2013. Hormone priming improves germination characteristics and enzyme activity of sorghum seeds (Sorghum bicolor L.) under accelerated aging. Cercetari Agronomice in Moldova. 3(155): 49-56.
Bailly, C. 2004. Active oxygen species and antioxidants in seed biology. Seed Sci. Res. 14: 93-107.
Behrouzyar, E.K. and M. Yarnia, 2014. Effect of ethanol, methanol, zinc, manganese and boron seed priming on ageing, seed germination and physiological characteristics in canola under water deficit stress. Res. Crops. 15(1):116-121.
Bradford, M.M. 1976. A dye binding assay for protein. Analytical Biochem.72: 248-254.
Cakmak, I., and W. Horst, 1991. Effect of aluminium on lipid peroxidation, superoxide dismutase, catalase and peroxidase activities in root tip of soybean (Glycine max). Plant Physiol. 83: 463–468.
Cavalcanti, F.R., J.T.A. Oliveira, A.S. Martins-Miranda, R.A. Viégas, and J.A.G. Silveira, 2004. Superoxide dismutase, catalase and peroxidase activities do not confer protection against oxidative damage in salt-stressed cowpeas leaves. New Phytol. 163: 563–571.
Delouche, J.C., and C.C. Baskin, 1973. Accelerated ageing technique for predicting relative storability of seed lots. Seed Sci. Technol. 1: 427-452.
Demir, I., C. Cebeci., and T. Guloksuz, 2012. Electrical conductivity measurement to predict germination of commercially available radish seed lots. Seed Sci. Technol. 40: 229–237.
Eisvand, H.R., R. Tavakkol-Afshari, F. Sharifzadeh, H. Maddah Arefi, and S.M. Hesamzadeh Hejazi, 2010. Effects of hormonal priming and drought stress on activity and isozyme profiles of antioxidant enzymes in deteriorated seed of tall wheatgrass (Agropyron elongatum Host). Seed Sci. Technol. 38: 280-297.
Ellis, R.A., and E.H. Roberts, 1981.The quantification of ageing and survival in orthodox seeds. Seed Sci. Technol. 9: 373–409.
Giannopolitis, C., and S. Ries, 1977. Superoxid desmutase. I: Occurence in higher plant, Plant Physiol. 59: 309–314.
Hampton, J.G., and D.M. TeKrony, 1995. Handbook of Vigour Test Methods. ISTA, Zurikh.
Heyl, A., M. Riefler, G. Romanov, and T. Schmulling, 2012. Properties, functions and evolution of cytokinin receptors. Europ. J. Cell Biol. 91: 246–256.
Hou, L., W. Liu,  Z. Li,  C. Huang, X.L. Fang, Q. Wang, and X. Liu, 2014. Identification and Expression Analysis of Genes Responsive to Drought Stress in Peanut. Russ. J. Plant Physiol. 61(6): 842–852.
Irigoyen, J.J., D.W. Emerich, and M. Sanchez-Diaz, 1992. Water stress induced changes in concentrations of proline and total soluble sugars in nodulated alfalfa (Medicago sativa) plants. Physiol. Plant. 84: 55-60.
ISTA. 2007. International Rules for Seed Testing. Seed Sci. Technol. 13: 299–520.
Jisha, K.C., K. Vijayakumari, and J.T. Puthur, 2013. Seed priming for abiotic stress tolerance: an overview. Acta Physiol. Plant. 35: 1381–1396.
Jyoti, and C.P. Malik, 2013. Seed deterioration: a review. Int. J. Life Sci. Biotech. Pharma Res. 2(3): 374–385.
Kapoor, R., A. Arya, M.A. Siddiqui, A. Amir, and H. Kumar, 2010. Seed Deterioration in Chickpea (Cicer arietinum L.) under Accelerated Ageing. Asian J. Plant Sci. 9(3): 158-162.
Khan, M.B., M.A. Gurchani, M. Hussain, S. Freed, and K. Mahmood, 2011.Wheat seed enhancement by vitamin and hormonal priming. Pak. J. Bot. 43: 1495–1499.
Kibinza, S., J. Bazin, C. Bailly, J.M. Farrant, F. Corbineau, and H. El-Marrouf Bouteau, 2011. Catalase is a key enzyme in seed recovery from ageing during priming. Plant Sci. 181: 309-315.
Michel, B.E., and M.R. Kaufmann, 1973. The osmotic potential of polyethylene glycol 6000. Plant Physiol. 51: 914-916.
Miransari, M., and D.L. Smith, 2014. Plant hormones and seed germination. Environ. Exp. Bot. 99: 110– 121.
Muller, B., and J. Sheen, 2007. Advances in cytokinin signaling. Science. 318(68):68-69.
Nakano, Y., and K. Asada, 1981. Hydrogen peroxide scavenged by ascrobate-specific peroxidase in spinach chloroplast. Plant Cell Physiol. 22: 867–880.
Nautiyal, P.C. 2009. Seed and seedling vigour traits in groundnut (Arachis hypogaea L.). Seed Sci. Technol. 37: 721-735.
Noorhosseini Niyaki, S.A., M.N. Safarzadeh Vishekaei, and S.M. Sadeghi, 2013. Potassium leachate and electrical conductivity tests efficiency in seed vigour evaluation of produced peanut in Astaneh Ashrafieh. (In Persian, with English Abstract.) J.Crop Prod. Res. 5 (1): 93-118.
Peleg, Z., and E. Blumwald, 2011. Hormone balance and abiotic stress tolerance in crop plants. Curr. Opin. Plant Biol. 14: 290–295.
Powell, A. 2010. Morphological and physiological characteristics of seeds and their capacity to germinate and survive. Ann. Bot. 105: 975–976.
Rahnama-Ghahfarokhi, A., and R. Tavakkol-Afshari, 2007. Methods for dormancy breaking and germination of galbanum seeds (Ferula gummosa). Asian J. Plant Sci. 6: 611-616.
Rehman, H., H. Iqbal, S.M.A. Basra, I. Afzal, M. Farooq, A. Wakeel, and W. Ning, 2015. Seed priming improves early seedling vigor, growth and productivity of spring maize. J. Integ. Agric. 14(9): 1745–1754.
Siadat, S.A., S.A. Moosavi, and M. Sharifzadeh, 2015. Alleviate Seed Ageing Effects in Silybum marianum by Application of Hormone Seed Priming. Not. Sci. Biol. 7(3): 316-321.
Tabatabaei, S.A. 2013. The Effect of priming on germination and enzyme activity of sesame (Sesamum indicum L.) seeds after accelerated aging. J. St. Physiol. Biochem. 9 (4): 132-138.
Taiz, L., and Zeiger, E. 2002. Plant Physiology, 3 edn. Sinauer Associates, Inc. Publishers, Sunderland, Massachusetts.
Tale Ahmad, S., and Haddad, R. 2011. Study of Silicon Effects on Antioxidant Enzyme Activities and Osmotic Adjustment of Wheat under Drought Stress. Czech J. Genet. Plant Breed. 47 (1): 17–27.
Varier, A., A.K., Vari, and M. Dadlani, 2010. The subcellular basis of seed priming. Curr. Sci. 99(4): 450-456.
Xia, F., X.Wang, M. Li,  and P. Mao, 2015. Mitochondrial structural and antioxidant system responses to aging in oat (Avena sativa L.) seeds with different moisture contents. Plant Physiol. Biochem. 94: 122-129.
Yan, M. 2015. Hydropriming promotes germination of aged napa cabbage seeds. Seed Sci. Technol. 43(2): 303-307.
Zhang, M., Z. Wang, L. Yuan, C. Yin, J. Cheng, L. Wang, J. Huang, and H. Zhang, 2014. Osmopriming improves tomato seed vigor under aging and salinity stress. Afric. J. Biotechnol. 11(23): 6305-6311.
Iranian Journal of Seed Science and Technology
Volume 5, Issue 2 - Serial Number 10
January 2016
Pages 181-198

Files

Share

How to cite

Statistics