Physiological-Biochemical Responses of Aged Groundnut Seeds to hydrogen peroxide priming during drought stress

Document Type : Original Article

Authors

1 دانشکده کشاورزی، گروه زراعت و اصلاح نباتات

2 Department of Agronomy and Plant Breeding, Faculty of Agriculture, Bu-Ali Sina University

Abstract

Seed deterioration is an important factor in reducing of physiological seed quality that it increased the severity of damage in adverse environmental conditions. The effects of hydrogen peroxide to improve physiological and antioxidant enzyme activities in aged groundnut seeds under drought stress were studied. A factorial experiment based on completely randomized design with three replications was done. Priming of different concentrations of hydrogen peroxide (0, 50, 100 and 150 µM) under 0, -0.4, -0.6 and -0.8MPa drought stress levels were evaluated. The results showed that seed priming with hydrogen peroxide in different drought levels increased some traits such as germination percentage, germination rate, vigour index, plumule and radicle length, soluble carbohydrates and protein contents and antioxidant enzymes activities (catalase, ascorbate peroxidase and superoxide dismutase). While it decreased mean germination time, electrical conductivity and malondialdehyde content in comparison to nonprime seeds. So seed priming with 50 and 100 µM of hydrogen peroxide in -0.8MPa level increased germination rate, vigor index , soluble carbohydrates and proteins, respectively by 27.89, 93.24, 71.42, 46.12, 27.06, 76.73, 30.26 and 51.04% compared to nonprimed seeds. Also, it increased activity of catalase, superoxide dismutase and ascorbate peroxidase, respectively by 26.37, 8.79, 39.07, 15.43, 5.28 and 21.51% compared to non-primed seeds. Therefore, the use of 50 mM hydrogen peroxide to reduce the negative effects caused by deterioration in drought stress is recommended.

Keywords

References

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Iranian Journal of Seed Science and Technology
Volume 9, Issue 2 - Serial Number 18
September 2020
Pages 1-17

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