Document Type : Original Article
Author
Associate professor, Imam Khomenini Higher Education Center, Agricultural Research, Education And Extension Organization, Tehran, Iran.
Abstract
Annual ornamental plants, mostly, propagated by seeds. Seeds of some these plants have difficulties in germination due to ecological adaptations to environmental conditions. In order to investigation about the effect of different treatments on the germination of three ornamental plants, Salvia grandiflora, Bellis perennis and Alyssum maritimum, three separate experiments were accomplished based on Completely Randomized Design by 4 treatments and 4 replications. Treatments include GA3 by three levels (100, 500, 1000 PPM), sulfuric acid 50% in three time (5, 10, 20 minutes), stratification during three weeks in three temperatures (0-2, 3-4, 5-6 ˚C) and control. Means comparison was done via Duncan Test by SPSS software. The results showed that gibberellic acid with a concentration of 1000 ppm had the greatest effect on the percentage and speed of Alyssum maritimum seed germination. Cold treatment did not have a significant effect on germination and sulfuric acid treatment caused a significant decrease in Alyssum maritimum seed germination. Different concentrations of gibberellic acid and chilling treatments did not have a significant effect on the germination of Bellis perennis seed, and sulfuric acid caused a significant decrease in germination. Gibberellic acid at the concentration of 1000 ppm increased the germination of Salvia grandiflora. The highest amount of germination in sage was observed in the treatment of gibberellic acid at the level of 1000 ppm (76.65%) and temperature 0-2, and sulfuric acid in 20 minutes.
Keywords
Aghilian, S., Khajeh-Hosseini, M., & Anvarkhah, S. (2014). Evaluation of seed dormancy in forty medicinal plant species. International Journal of Agriculture and Crop Sciences, 7(10), 760–768.
Al-Safadi, B., & Elias, R. (2011). Improvement of caper (Capparis spinosa L.) propagation using in vitro culture and gamma irradiation. Scientia Horticulturae, 127(3), 290–297. https://doi.org/10.1016/j.scienta.2010.10.014
Bayat, H., & Aminifard, M. H. (2018). Effects of salinity and drought stresses on seed germination, growth, and proline content of common daisy (Bellis perennis L. "Bellissima mixture") seedling. Iranian Journal of Seed Science and Research, 5(2), 83–93. https://doi.org/10.22124/jms.2018.2913 [In Persian]
Benech-Arnold, R. L., Sanchez, R. A., Forcella, F., Kruk, B. C., & Ghersa, C. M. (2000). Environmental control of dormancy in weed seed banks in soil. Field Crops Research, 67, 105–122. https://doi.org/10.1016/S0378-4290(00)00087-3
Bond, W., Davies, G., & Turner, R. (2007). The biology and non-chemical control of Bellis perennis L. [PDF document]. https://www.gardenorganic.org.uk/sites/www.gardenorganic.org.uk/files/organic-weeds/bellis-perennis.pdf
Estaji, A., Hosseini, B., Dehghan, E., & Pirzad, A. (2012). Seed treatment to overcome dormancy of Salvia leriifolia Bent. International Research Journal of Applied and Basic Sciences, 3(10), 2003–2008.
Fariman, Z. K., Azizi, M., & Noori, S. (2011). Seed germination and dormancy breaking techniques for Echinacea purpurea L. Journal of Biological and Environmental Sciences, 5(13), 7–10.
Fateh, E., Malekpourlashkariani, N., & Gerami, F. (2012). Effects of various treatments on Echinacea purpurea L. seed dormancy breaking and germination. International Journal of Plant, Animal and Environmental Sciences, 2(3), 188–194.
Finch-Savage, W. E., & Leubner-Metzger, G. (2006). Seed dormancy and the control of germination. New Phytologist, 171(3), 501–523. https://doi.org/10.1111/j.1469-8137.2006.01787.x
Finch-Savage, W. E., & Bassel, G. W. (2016). Seed vigour and crop establishment: Extending performance beyond adaptation. Journal of Experimental Botany, 67(3), 567–591. https://doi.org/10.1093/jxb/erv490
Finkelstein, R., Reeves, W., Ariizumi, T., & Steber, C. (2008). Molecular aspects of seed dormancy. Annual Review of Plant Biology, 59, 387–415. https://doi.org/10.1146/annurev.arplant.59.032607.092740
Gupta, S. M., Pandey, P., Grover, A., & Ahmed, Z. (2011). Breaking seed dormancy in Hippophae salicifolia, a high-value medicinal plant. Physiology and Molecular Biology of Plants, 17(4), 403–406. http://doi.org/10.1007/s12298-011-0082-6
Kalateh, M., Kazemi, F., & Samiei, L. (2022, March 14). Investigating the effect of various factors on germination indices of Salvia species. In The Fifth National Conference on New Technologies in Agriculture, Natural Resources and Environment. Tehran, Iran. https://civilica.com/doc/1472194.pdf [In Persian]
Keshtkar, H. R., Azarnivand, H., Etemad, V., & Moosavi, S. S. (2008). Seed dormancy-breaking and germination requirements of Ferula ovina and Ferula gummosa. Desert, 13, 45–51. https://doi.org/10.22059/JDESERT.2008.27174
Khakpoor, A., Habibi Bibalani, Gh., & Mahdavi, Kh. (2011). Influence of scabbing and hot water treatment on germination of Salvia verticillata seeds. Annals of Biological Research, 2(5), 52–55.
Khakpoor, A., Habibi Bibalani, Gh., & Mahdavi, Kh. (2013). Breaking seed dormancy and germination stimulation in Salvia verticillata. Natural Ecosystems of Iran, 3(1), 12–17.
Khakpoor, A., Habibi Bibalani, Gh., & Mahdavi, Kh. (2015). Optimal treatment increased the seed germination of Salvia verticillata L. Journal of BioScience & Biotechnology, 4(3), 255–261.
Kumari, P., & Dahiya, O. S. (2007). Dormancy, seed viability, and seed vigour. In S. S. Narwal, B. Politycka, & C. L. Goswami (Eds.), Plant physiology research methods (pp. 41–57). Scientific Publishers.
Kwon, H. J., Shin, S. L., Kim, Y. R., & Kim, S. Y. (2020). Effects of temperature, gibberellic acid, and KNO₃ treatments on seed germination of Maesa japonica. Seed Science and Technology, 48(1), 65–72. https://doi.org/10.15258/sst.2020.48.1.09
Lu, J. J., Tan, D. Y., Baskin, J. M., & Baskin, C. C. (2010). Fruit and seed heteromorphism in the cold desert annual ephemeral Diptychocarpus strictus (Brassicaceae) and possible adaptive significance. Annals of Botany, 105(6), 999–1014. https://doi.org/10.1093/aob/mcq041
Mello, A. M., Streck, N. A., Blankenship, E. E., & Paparozzi, E. T. (2009). Gibberellic acid promotes seed germination in Penstemon digitalis cv. Husker Red. HortScience, 44(3), 870–873. https://doi.org/10.21273/HORTSCI.44.3.870
Mobli, A., Mijani, S., Ghanbari, A., & Rastgoo, M. (2019). Seed germination and emergence of two flax-leaf alyssum (Alyssum linifolium Steph. Ex. Willd.) populations in response to environmental factors. Crop and Pasture Science, 70(9), 807–813. https://doi.org/10.1071/CP19162
Nadjafi, F. M., Bannayan, L., Tabrizi, M., & Rastgoo, M. (2006). Seed germination and dormancy-breaking techniques for Ferula gummosa and Teucrium polium. Journal of Arid Environments, 64(3), 542–547. https://doi.org/10.1016/j.jaridenv.2005.06.009
Ozcan, I. I., Arabaci, O., & Oğretmen, N. G. (2014). The determination of different germination applications on some sage species. Turkish Journal of Agricultural Food Science & Technology, 2(5), 203–207.
Panwar, P. B., & Hardwaj, S. D. (2005). Handbook of practical forestry. Agrobios.
Penfield, S., Meissner, R. C., Shoue, D. A., Carpita, N. C., & Bevan, M. W. (2001). MYB61 is required for mucilage deposition and extrusion in the Arabidopsis seed coat. The Plant Cell, 13(12), 2777–2791. https://doi.org/10.1105/tpc.010265
Rahimi, H., Malek, M., & GhaderiFar, F. (2022). Seed dormancy: A review on importance, dormancy types, and elimination methods, with emphasis on the trend of seed dormancy research in Iran. Iranian Journal of Seed Research, 8(2), 131–150. https://sid.ir/paper/1137141/en [In Persian]
Rouhi, H. R., Karimi, F. A., Shahbodaghlo, A. R., Sheikhalian, M., Rahmatabadi, R., & Samadi, M., & Karimi, F. (2012). Effects of sulfuric acid, stratification, phytohormone, and potassium nitrate on dormancy breaking and germination of Tulipa kaufmanniana Regel. International Journal of AgriScience, 2(2), 136–142.
Sadeghi, H., & Rasouli, M. (2012). Effective methods for improving seed germination of Medicago scutellata and Medicago rigidula. Romanian Agricultural Research, 29, 37–41.
Sun, Y., Tan, D. Y., Baskin, C. C., & Baskin, J. M. (2012). Role of mucilage in seed dispersal and germination of the annual ephemeral Alyssum minus (Brassicaceae). Australian Journal of Botany, 60(5), 439–449. https://doi.org/10.1071/BT11314
Tuncer, B., & Ummuhan, F. (2017). Research on overcoming the dormancy problem of Corchorus olitorius L. seeds. Turkish Journal of Agricultural Research, 4(3), 268–274. http://dergipark.gov.tr/download/article-file/334867
Tursun, A. O. (2020). The effects of low temperature applications on dormancy of Salvia verticillata L. and Rumex crispus L. seeds. Pakistan Journal of Botany, 52(6), 1885–1890. https://doi.org/10.30848/PJB2020-6(18)
Wester, T. L. (2012). The sticky tale of seed coat mucilages: Production, genetics, and role in seed germination and dispersal. Seed Science Research, 22(1), 1–25. https://doi.org/10.1017/S0960258511000249
Zammali, I., Dabbous, A., Youssef, S., & BenHamed, K. (2022). Effects of chemical priming on the germination of the ornamental halophyte Lobularia maritima under NaCl salinity. Seeds, 1(2), 99–109. https://doi.org/10.3390/seeds1020009
Zutić, I., & Dudai, N. (2008). Factors affecting germination of Salvia officinalis seed. Acta Horticulturae, 782, 121–126. https://doi.org/10.17660/ActaHortic.2008.782.12
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