Radman Karimi; Farshid Ghaderi-Far; A. Hamidi; Mohsen Malek; Hamid Reza Sadeghipour; Asieh Siahmarguee
Abstract
This research was conducted to investigate the effects of coating wheat cv. dryland Qaboos seeds by the liquid superabsorbent polymer AB200-c under water stress conditions. The factors of the experiments included different water potentials of 0, -0.3, -0.6,, -0.9 and -1.2 MPa and seed coating by superabsorbent ...
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This research was conducted to investigate the effects of coating wheat cv. dryland Qaboos seeds by the liquid superabsorbent polymer AB200-c under water stress conditions. The factors of the experiments included different water potentials of 0, -0.3, -0.6,, -0.9 and -1.2 MPa and seed coating by superabsorbent polymer at 0, 0.2, 0.5, 0.7 and 2 ml of super absorbent polymer per 100 grams seeds. The Hydrotime model based on binomial distribution was used to investigate the reaction of seed germination to different coating treatments and water stress. Seed coating by the superabsorbent polymer increased germination percentage compared to control seeds at water potentials of -0.9 and -1.2 MPa. Also, these treatments increased the growth rate of seedlings compared to control. Based on the analysis of the coefficients in the Hydrotime model, seeds coated by the superabsorbent polymer made more negative base water potential or greater tolerance to water stress; the value of this coefficient was -1.54 MPa in the control seeds but -1.83 MPa in the coated seeds. Accordingly, polymer coating increased the threshold of tolerance to water stress in wheat seeds. Also, differential coating of seeds with the superabsorbent polymer had significant effects on germination and seedling growth criteria, which point to the importance of using an appropriate amount of superabsorbent polymer in wheat seed coating treatment. Based on this research, seed coating with the superabsorbent polymer can be considered as an efficient technique for reducing the negative effects of water stress on wheat seed germination and seedling growth.
Seyed Abbas Mirjalili
Abstract
Annual ornamental plants, mostly, propagated by seeds. Seeds of some these plants have difficulties in germination due to ecological adaptations to environmental conditions. In order to investigation about the effect of different treatments on the germination of three ornamental plants, Salvia grandiflora, ...
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Annual ornamental plants, mostly, propagated by seeds. Seeds of some these plants have difficulties in germination due to ecological adaptations to environmental conditions. In order to investigation about the effect of different treatments on the germination of three ornamental plants, Salvia grandiflora, Bellis perennis and Alyssum maritimum, three separate experiments were accomplished based on Completely Randomized Design by 4 treatments and 4 replications. Treatments include GA3 by three levels (100, 500, 1000 PPM), sulfuric acid 50% in three time (5, 10, 20 minutes), stratification during three weeks in three temperatures (0-2, 3-4, 5-6 ˚C) and control. Means comparison was done via Duncan Test by SPSS software. The results showed that gibberellic acid with a concentration of 1000 ppm had the greatest effect on the percentage and speed of Alyssum maritimum seed germination. Cold treatment did not have a significant effect on germination and sulfuric acid treatment caused a significant decrease in Alyssum maritimum seed germination. Different concentrations of gibberellic acid and chilling treatments did not have a significant effect on the germination of Bellis perennis seed, and sulfuric acid caused a significant decrease in germination. Gibberellic acid at the concentration of 1000 ppm increased the germination of Salvia grandiflora. The highest amount of germination in sage was observed in the treatment of gibberellic acid at the level of 1000 ppm (76.65%) and temperature 0-2, and sulfuric acid in 20 minutes.
Bita Oskouei; Leila Sadeghi; Kobra Moslemkhani; Hadis Afshar; Zahra Radmanesh
Abstract
Genetic purity, as one of the important characteristics of the quality of seed lots, has been considered in the national standards of certified seed producing countries to achieve desirable and stable characteristics of agricultural cultivars. According to the national standard, determining the other ...
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Genetic purity, as one of the important characteristics of the quality of seed lots, has been considered in the national standards of certified seed producing countries to achieve desirable and stable characteristics of agricultural cultivars. According to the national standard, determining the other speciese in the seed sample is considered as one of the important tests of seed quality. This feature is checked with an emphasis on recognizable characteristics in the seed analysis laboratory. The research has investigated the characteristics and differences in appearance and genetics of seeds off- type of chickpea seeds. The results showed that the seeds that were considered as off- type morphologically differed from the control in at least one of the measured traits. These seeds were different from the original seed in terms of color, shape seed surface roughness and seed groove, also the selected indices were similar in the first and second generation seeds. The results of the molecular test also confirmed the results of the morphological investigations. In Adel, Mansour, Arman cultivars, the samples that in morphological studies were considered as off-type, in molecular studies using microsatellite markers H3F09, H3C11 and H1A06 showed that the molecular profile was not uniform and allele diversity was greater than the main allele of cultivars. In the process of seed certification program, microsatellite markers are a suitable and complementary tool to the use of morphological markers to determine the genetic purity of chickpea seed samples.
Reza Rezvani; Behnam Kamkar; Zeinab Jabbari Badkhor
Abstract
To mitigate the detrimental effects of salinity stress on vegetable crops, various approaches, including the applying of organic inputs during different growth stages, such as germination, are employed. This study, conducted in 2023, aimed to assess the influence of humic acid pretreatment on the germination ...
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To mitigate the detrimental effects of salinity stress on vegetable crops, various approaches, including the applying of organic inputs during different growth stages, such as germination, are employed. This study, conducted in 2023, aimed to assess the influence of humic acid pretreatment on the germination characteristics and growth parameters of cucumber (Cucumis sativus) under salt stress. Two experiments, one in the laboratory and the other in a greenhouse, were carried out using a factorial design based on a completely randomized design with three replications. The experimental factors included humic acid priming at four concentrations (0, 50, 100, and 200 mg/l-1) and salinity at five levels (0, 25, 50, 100, and 150 mM NaCl). Results indicated that humic acid exerted a positive impact on various germination and growth attributes of cucumber, including germination percentage, germination rate, seedling length, dry weight, vigor index, and chlorophyll a, b, and carotenoid under salt-stressed conditions compared to the control. The treatment with 200 mmol/l humic acid and no salt exhibited the highest germination rate (87%) and seedling length (12.66 cm). The interaction between salinity and humic acid revealed that humic acid concentrations exceeding 100 mg/l-1 could alleviate the adverse effects of salinity stress on total chlorophyll. In conclusion, the cost-effective and easily applicable method of priming cucumber seeds with humic acid presents a potential strategy to mitigate the impacts of salinity stress in regions with saline water or soil.
Zahra Yaghobian; Seyed Hossein Nemati; Ehsan Nematollahi
Abstract
To investigate the effect of seed coating on characteristics related to germination of lettuce seeds under drought stress conditions, a factorial experiment was conducted a completely randomized design with 4 replications during 2022. The experimental treatments include seed coating(C1:uncoated seeds ...
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To investigate the effect of seed coating on characteristics related to germination of lettuce seeds under drought stress conditions, a factorial experiment was conducted a completely randomized design with 4 replications during 2022. The experimental treatments include seed coating(C1:uncoated seeds (control), C2:seed coating with kaolin+gibberellin,C3:seed coating with kaolin+humic acid+gibberellin, C4:seed coating with gibberellin+micro elements+kaolin, C5:seed coating with gibberellin+ macro elements + kaolin, C6:seed coating with humic acid+micro+macro elements+ kaolin, C7:seed coating with gibberellin + humic acid+ micro + macro elements + kaolin and it was evaluated under three levels of drought stress(no stress(control),-0.4 MPa,- 0.8 MPa). In the stress condition of -0.8 MPa, only two treatments C2 (16%) and C3 (8%) had germination. In the stress of 0.4 MPa, treatments C1, C2, C3, respectively, in the characteristics of germination percentage (88.50, 61.75 and 27.50), germination speed (6.46, 3.91 and 2.25) and root index The seeds (130/28, 93/32 and 44/02) had significant differences with each other and also with non-stressed conditions (control); So that C1 seed (control) had 72.5% and C3 seed had 11.5% reduction in germination compared to non-stressed conditions. C3 coating improved the average germination time compared to C1 coating (3.07). The results of the experiment showed that drought stress is a limiting factor in the germination of lettuce seeds by limiting water absorption, and some treatments such as gibberellin+ kaolin coating and gibberellin+ kaolin+ humic acid improve the characteristics related to lettuce seed germination.
Parvin Salehi Shanjani; Hamideh Javadi; Leila Rasoulzadeh; Leila Falah Hoseini; Arash Mamedi
Abstract
To investigate the cardinal temperature of three medicinal species of Nepata haussknechtii, N. pogonosperma and N. glomerulosa subsp. staffina and determining the appropriate temperature and time conditions for their planting, a factorial experiment was carried out in the form of a completely randomized ...
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To investigate the cardinal temperature of three medicinal species of Nepata haussknechtii, N. pogonosperma and N. glomerulosa subsp. staffina and determining the appropriate temperature and time conditions for their planting, a factorial experiment was carried out in the form of a completely randomized design in three replications in 2023 in the Seed Technology Research Laboratory of the Gene Bank of Natural Resources of Iran. Three non-linear regression models, including dent-like, segmented and beta, were evaluated at eight temperature levels (5, 10, 15, 20, 25, 30, 35 and 40°C) to describe the response of the seed germination rate of three Nepeta species to temperature. Statistical indices such as root mean square (RMSE) and coefficient of explanation (R2) were used to compare the models.The results showed that the parameters estimated using the dent-like model in N. haussknechtii include base temperature, optimal lower temperature, optimal upper temperature, ceiling temperature, R2 and RMSE values were 9.01°C, 20°C, 25°C, 36.21°C, 0.94 and 0.11, respectively; segmented model in the N. pogonosperma include base temperature, optimal temperature, ceiling temperature, R2 and RMSE values were 9°C, 23°C, 36.14°C, 0.95 and 0.31, respectively; and beta model in the N. glomerulosa subsp. staffina include base temperature, optimal temperature, ceiling temperature, R2 and RMSE values were 9.84°C, 24.95°C, 37°C, 0.99 and 0.03, respectively had more confidence. The results of this experiment showed that in the different studied species of Nepeta, due to the similar initial growth rate, they have the same germination peak.
