Document Type : Original Article

Authors

1 M.Sc. Student in Agroecology of Plant Production and Genetic Department, Shirvan Faculty of Agriculture, University of Bojnord, Bojnord, Iran

2 Professor, Faculty Member of Plant Production and Genetic Department, Shirvan Faculty of Agriculture, University of Bojnord, Bojnord, Iran.

3 Research Assistant of Plant Production and Genetic Department, Shirvan Faculty of Agriculture, University of Bojnord, Bojnord, Iran.

10.22092/ijsst.2024.365149.1517

Abstract

Two factorial experiments in different conditions, laboratory and greenhouse, were conducted based on a completely randomized design and randomized complete block design with three replications, respectively, to investigate the effect of aqueous extract of aerial organs of Ephedra major Host. on the growth traits and germination of Triticum aestivum, Avena fatua and Sinapis arvensis The first factor was plant species including :Triticum aestivum, Avena fatua and Sinapis arvensis and the second factor was aqueous extract of ephedra plant in three levels (zero (control), 10 and 20% of aerial parts (including stem and leaves mixed). The results showed that with increasing extract concentration, the germination, growth traits and total chlorophyll of all investigated plants significantly decreased. The 20% concentration of aqueous extract of ephedra decreased the germination rate in wheat, oat and mustard by 51.8% and 42.8%, 53.2% and 61.5%, 80% and 50.3%, respectively, compared to the control. Also, 20% concentration of ephedra extract had highest inhibition on dry weight of wheat (52%), oat (68.3%) and wild mustard (83.5%) compared to control. The results of experiment showed that oat plants were more sensitive to aqueous extract of Ephedra so that chlorophyll content of oat plants 53% decreased compared to control. In general, weed plants were more sensitive to the allelopathic effects of ephedra than wheat. In fact, it is possible that the germination and growth of the mentioned weeds can be moderated with the cheap and easy application of ephedra aerial part extract.

Keywords

Ahmadvand, G., Koocheki, A., & Nassiri Mahallati, M. (2003). Competitive response of winter wheat (Triticum aestivum) to various plant densities of wild oat (Avena ludoviciana) and nitrogen fertilizer. Journal of Agricultural Science and Technology, 1, 113–124. (In Persian)
Asgharipour, M. R., Rashed Mohassel, M. H., Rostami, M., & Eizadi, E. (2015). The allelopathic potential of saffron (Crocus sativus L.) on following crop in rotation. In Proceedings of the International Symposium on Saffron Biology and Technology (p. 48). Mashhad, Iran.
Ahn, J., & Chung, I. (2000). Allelopathic potential of rice hull on germination and seedling growth of barnyardgrass. Agronomy Journal, 92(6), 1162–1167. https://doi.org/10.2134/agronj2000.9261162x
Alipour Garavand, S., Amini Dehaghi, M., & Ahmadi, K. (2017). Evaluation of the allelopathic effect of extracts of bindweeds and mallow on germination characteristics and growth parameters of three sesame cultivars. Journal of Seed Research, 8(29), 7–14. (In Persian) 
Arnon, D. I. (1967). Method of extraction of chlorophyll in plants. Plant Physiology, 23, 112–121.
Bayat, H., Naseri Moghaddam, A., & Aminifard, M. (2020). Allelopathic effects of narcissus (Narcissus tazetta L.) extract on germination, growth, and physiological characteristics of couch grass (Agropyron repens) and wild oat (Avena fatua). Journal of Seed Science Research, 6(4), 457–469. https://doi.org/10.22124/JMS.2020.3925 (In Persian)
Barmaki, M. (2019). Study of the allelopathic effect of saline grass on germination and heterotrophic seedling growth of some crops. Journal of Plant Ecosystem Conservation, 6(12), 135–152. (In Persian)
Bachheti, A., Sharma, A., Bachheti, R. K., Husen, A., & Pandey, D. (2020). Plant allelochemicals and their various applications. In J. M. Mérillon & K. G. Ramawat (Eds.), Co-evolution of secondary metabolites (pp. 10–38). Springer.
Bond, W., & Turner, R. (2006). The biology and non-chemical control of common amaranth (Amaranthus retroflexus L.). John Wiley & Sons.
Chauhan, B. S. (2020). Grand challenges in weed management. Frontiers in Agronomy, 1, Article 3. https://doi.org/10.3389/fagro.2019.00003
Cianfaglione, K., Crisan, F., & Gafta, D. (2023). Soil quality enhances seed germination success in Ephedra major: A pilot experiment. Plants, 12(3), 438. https://doi.org/10.3390/plants12030438
Clapp, J. (2021). Explaining growing glyphosate use: The political economy of herbicide-dependent agriculture. Global Environmental Change, 67, 102239. https://doi.org/10.1016/j.gloenvcha.2021.102239
Dadkhah, A. (2012). Allelopathic effect of Ephedra major on growth and photosynthesis of Cirsium arvense. International Journal of Agriculture, 2(4), 416–419.
Esfandiari, S., Dadkhah, A., & Rezvani, R. (2023). Investigation of the allelopathic potential of Zygophyllum eurypterum on seed germination and seedling growth indices of Triticum aestivum and Acroptilon repens. Journal of Seed Science and Technology, 12(3), 79–92. https://doi.org/10.22092/ijsst.2023.361380.1474 (In Persian)
Farhoudi, R., & Lee, D. (2013). Allelopathic effects of barley extract (Hordeum vulgare) on sucrose synthase activity, lipid peroxidation, and antioxidant enzymatic activities of Hordeum spontaneum and Avena ludoviciana. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences, 83, 447–452. https://doi.org/10.1007/s40011-012-0137-7
Farhoudi, R. (2014). Investigation of the allelopathic effects of aqueous extracts of barley on germination and seedling electrical leakage of Lolium multiflorum and Avena ludoviciana. Journal of Applied Field Crops Research, 1(4), 17–21. (In Persian)
Ghasemi Arian, A., Ghorbani, R., Nasripour Yazdi, M., & Mesdaghi, M. (2016). Effect of temperature on seed germination characteristics of Dorema ammoniacum. Iranian Journal of Biology, 29(3), 686–693. https://doi.org/20.1001.1.23832592.1395.29.3.20.6 (In Persian)
Hatami Hampa, A., Javanmard, A., Alebrahim, M., & Sofalian, O. (2018). Allelopathic effects of aqueous extracts from sorghum (Sorghum bicolor L.) and Russian knapweed (Acroptilon repens L.) on seedling growth and enzyme activity of wheat, sugar beet, common lambsquarters, and redroot pigweed. Iranian Journal of Plant Protection Research, 32(1), 101–119. https://doi.org/10.22067/jpp.v32i1.62909 (In Persian)
Ikic, I., Maricevic, M., Tomasovic, S., Gunjaca, J., Sarcevic, Z., & Arcevic, H. (2012). The effect of germination temperature on seed dormancy in Croatia-grown winter wheats. Euphytica, 188, 25–34. https://doi.org/10.1007/s10681-012-0735-8
Korres, N. E. (2017). Agronomic weed control: A trustworthy approach for sustainable weed management systems. In K. Jabran & B. S. Chauhan (Eds.), Non-chemical weed control (pp. 97–114). Academic Press. https://doi.org/10.1016/B978-0-12-809881-3.00006-1
Khalili Mahalleh, J., Jalili, F., & Hosseini, N. (2014). Effect of four kinds of allelopathic weeds on the germination and growth of forage sorghum. Journal of Research in Crop Science, 5(20), 107–122. (In Persian)
Lowry, C. J., & Smith, R. G. (2018). Weed control through crop plant manipulations. In K. Jabran & B. Chauhan (Eds.), Non-chemical weed control (pp. 73–96). Academic Press.
Makizadeh Tafti, M., & Farhoudi, R. (2017). Investigation on the effect of aqueous barley extract on seedling growth and stability of the cell membrane in wild oat and ryegrass seedlings. Journal of Plant Production Sciences, 7(1), 66–72. (In Persian)
Makizadeh Tafti, M., Salimi, M., & Farhoudi, R. (2008). Investigating the allelopathic effect of the medicinal plant sedab (Ruta graveolens L.) on seed germination of three weed species. Iranian Journal of Medicinal and Aromatic Plants Research, 24(4), 463–471. (In Persian)
Nabati Souha, L., Alebrahim, M. T., Ahmadnia, F., & Babaei, A. (2021). Effect of different dormancy-breaking methods on germination characteristics of wild mustard seed (Sinapis arvensis L.). Journal of Seed Research, 10(4), 53–64. (In Persian)
Oracz, K., Bailly, C., Gniazdowska, A., Come, D., Corbineau, D., & Bogatek, R. (2007). Induction of oxidative stress by sunflower phytotoxins in germinating mustard seeds. Journal of Chemical Ecology, 33, 251–264.
Rodríguez Araujo, M. E., Milano, C., & Pérez, D. R. (2019). Germination of Ephedra ochreata Miers: Contribution for productive restoration of arid environments in Argentina. Agrociencia, 53, 617–629.
Rezvani, R., & Dadkhah, A. (2023). The effect of aqueous extracts of different organs of Peganum harmala L. on the germination and growth of Amaranthus retroflexus L. and Chenopodium album L. Iranian Journal of Seed Science and Technology, 12(1), 1–14. https://doi.org/10.22092/ijsst.2022.359764.1451 (In Persian)
Samedani, B., & Baghestani, M. A. (2005). Comparison of allelopathic activity of different Artemisia species on seed germination rate and seedling growth of Avena ludoviciana. Pajouhesh and Sazandegi, 68, 69–74. (In Persian)
Safahani Langroudi, A. R., & Ghoshchi, F. (2014). Allelopathic effects of aqueous extracts and residues of different weeds on germination and seedling growth of wheat. Journal of Plant Research (Iranian Journal of Biology), 27(1), 100–109. (In Persian)
Salahi, M., Abedi, B., Morshadloo, M., Ahangarani, M., Jabbari GhaleKhaki, S., Asghari, Z., & Esmaeili, E. (2021). The inhibitory effect of walnut and elderberry hydro-alcoholic extracts on germination and morphological and biochemical characteristics of Portulaca oleracea. Journal of Seed Science and Research, 7(4), 477–489. https://doi.org/10.22124/jms.2020.4644 (In Persian)
Saberi, M., Shahriari, A., Jafari, M., Tarnian, F., & Safari, H. (2012). Allelopathic effect of Thymus kotschyanus on seed germination and initial growth of Bromus inermis and Agropyron elongatum. Journal of Watershed Management Research, 93, 18–25. (In Persian)
Saraei, R., Lahouti, M., & Ganjeali, A. (2012). Evaluation of allelopathic effects of eucalyptus (Eucalyptus globulus Labill.) on germination and morphological and biochemical traits of barley (Hordeum vulgare) and flixweed (Descurainia sophia L.). Journal of Agriculture, 4, 215–222. https://doi.org/10.22067/jag.v4i3.15310 (In Persian)
Shahzad, M., Jabran, K., Hussain, M., Raza, M. A. S., Wijaya, L., El-Sheikh, M. A., & Alyemeni, M. (2021). The impact of different weed management strategies on weed flora of wheat-based cropping systems. PLoS ONE, 16(2), e0247137. https://doi.org/10.1371/journal.pone.0247137
Sharma, S., Kaur, R., & Kaur, N. (2019). Allelopathy and its role in agriculture. Journal of Pharmacognosy and Phytochemistry, 8(1), 274–277.
Singh, S., Buttar, G. S., Singh, S. P., & Brar, D. S. (2005). Effect of different dates of sowing and row spacing on yield of fenugreek (Trigonella foenum-graecum L.). Journal of Medicinal and Aromatic Plant Sciences, 27(4), 629–630.
Tahamizarandi, M. K., & Rezvani-Moghadam, P. (2011). Investigation of germination and seedling morphological characteristics of wild oat (Avena ludoviciana) under aqueous extract of aerial parts of medicinal plants. Iranian Journal of Field Crop Science, 25, 398–406. (In Persian)
Tan, K., Huang, Z., Ji, R., Qiu, Y., Wang, Z., & Liu, J. (2019). A review of allelopathy on microalgae. Microbiology, 165(6), 587–592. https://doi.org/10.1099/mic.0.000776
Türkmen, N., Sarı, F., & Velioğlu, Y. S. (2005). The effect of cooking methods on total phenolics and antioxidant activity of selected green vegetables. Food Chemistry, 93, 713–718. https://doi.org/10.1016/j.foodchem.2004.12.038
Yan, Z. Q., Wang, D. D., Ding, L., Cui, H. Y., Jin, H., Yang, X. Y., Yang, J. S., & Qin, B. (2015). Mechanism of artemisinin phytotoxic action: Induction of reactive oxygen species and cell death in lettuce seedlings. Plant Physiology and Biochemistry, 88, 53–59. https://doi.org/10.1016/j.plaphy.2015.01.010
Zou, J. N., Jin, X. J., Zhang, Y. X., Ren, C. Y., Zhang, M. C., & Wang, M. X. (2019). Effects of melatonin on photosynthesis and soybean seed growth during grain filling under drought stress. Photosynthetica, 57(2), 512–520. https://doi.org/10.32615/ps.2019.066