Document Type : Original Article
Authors
1 Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Kerman, Iran.
2 Expert of National Botanical Garden of Iran, Tehran, Iran
3 Expert of National Botanical Garden of Iran, Tehran, Iran.
10.22092/ijsst.2025.367891.1549
Abstract
To assess the impact of melatonin pretreatment on germination indices, initial growth, and α-amylase activity in Camelina (Camelina sativa L.) Soheil cultivar seedlings under osmotic stress, an experiment was conducted in a factorial arrangement based on completely randomized design in the research laboratory of Bardsir Agricultural Higher Education Center, Shahid Bahonar University of Kerman in 2023. The first factor was melatonin at three concentrations (0, 100, and 200 micromolar), while the second factor was polyethylene glycol solution (PEG6000) at seven levels (-0.2, -0.4, -0.6, -0.8, -1.0, and -1.2 MPa) with three replicates. The highest germination percentage (92.7%), shoot length (18.9 mm), root dry weight (1 mg/plant), and seedling dry weight (1.2 mg/plant) were observed under non-osmotic stress. Moreover, a 100 μM melatonin concentration increased germination percentage, shoot length, root dry weight, and seedling dry weight by approximately 31.4%, 27.1%, 36.9%, and 35.1%, respectively, compared to plants pretreated with distilled water. By decreasing the osmotic potential from 0 to -1 MPa, the germination rate, seedling vigor length index, root length, shoot dry weight and α-amylase enzyme activity in seeds pretreated with 100 µmol melatonin increased by about 36.8, 77.2%, 58.23%, 62.5% and 75.7%, respectively, compared to seeds pretreated with distilled water. In seed pretreatment with 200 µM melatonin under osmotic stress of -1.2 MPa, germination occurred over a period of ten days but no seedling growth was recorded. It seems that increasing the melatonin concentration was unable to mitigate the negative and inhibitory effects of -1.2 MPa osmotic stress.
Keywords
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