Bita Oskouei; Leila Sadeghi; Kobra Moslemkhani; Hadis Afshar; Zahra Radmanesh
Abstract
Genetic purity, as one of the important characteristics of the quality of seed lots, has been considered in the national standards of certified seed producing countries to achieve desirable and stable characteristics of agricultural cultivars. According to the national standard, determining the other ...
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Genetic purity, as one of the important characteristics of the quality of seed lots, has been considered in the national standards of certified seed producing countries to achieve desirable and stable characteristics of agricultural cultivars. According to the national standard, determining the other speciese in the seed sample is considered as one of the important tests of seed quality. This feature is checked with an emphasis on recognizable characteristics in the seed analysis laboratory. The research has investigated the characteristics and differences in appearance and genetics of seeds off- type of chickpea seeds. The results showed that the seeds that were considered as off- type morphologically differed from the control in at least one of the measured traits. These seeds were different from the original seed in terms of color, shape seed surface roughness and seed groove, also the selected indices were similar in the first and second generation seeds. The results of the molecular test also confirmed the results of the morphological investigations. In Adel, Mansour, Arman cultivars, the samples that in morphological studies were considered as off-type, in molecular studies using microsatellite markers H3F09, H3C11 and H1A06 showed that the molecular profile was not uniform and allele diversity was greater than the main allele of cultivars. In the process of seed certification program, microsatellite markers are a suitable and complementary tool to the use of morphological markers to determine the genetic purity of chickpea seed samples.
Ali Shayanfar; Samad Mobasser; Bita Oskouei; Leila Zare
Abstract
Camelina Cv. Soheil seed storability was assessed to simulate after-harvest condition till the following growing season. Seven seed samples were collected from Shirvan, Fasa, Marvdasht, Kermanshah, Hamedan, Sabzevar, and Ilam regions and saved for six months in two storage condition (with two temperatures ...
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Camelina Cv. Soheil seed storability was assessed to simulate after-harvest condition till the following growing season. Seven seed samples were collected from Shirvan, Fasa, Marvdasht, Kermanshah, Hamedan, Sabzevar, and Ilam regions and saved for six months in two storage condition (with two temperatures of 10 and 25ºC). Then, they were drow out monthly from storage condition to evaluate different seed germination index and vigour. Moisture seed content did not acced more than 10 percent in all treatments. Germination percentage and normal seedlings were more than 94% after 6 months of storage in all seed samples and two temperatures. Weight seedling vigour showed no significant differences in all samples, except the Sabzevar sample. The effects of three factors (seed samples, temperature and storage time) on rate germination index were difference. Seed physiological responses (in terms of rate germination) to 10 and 25ºC temperatures were similar until the fourth months in more samples. However, a declining process was observed from the fourth month at 25ºC and from the fifth month at 10ºC for these responses and accend minimum in six months. Two seed samples of Hamedan and Kermanshah showed higher rate of germination. In total, Camelina seeds showed high storage potential due to high germination percentage was recorded after six months of storage. In addition, if seeds stored for more than 4 months, plants in field condition lower germination rate will observe.
Ali Shayanfar; Bita Oskouei; Abbas Dehshiri; Hadis Afshar; Elahe Gheysari
Abstract
Randomized factorial design was implemented in four replications to determine optimal methods of Ajwain (Carum capticum) standard seeds germination. Ajwain seeds were placed top and between paper and sand exposed to two potassium nitrate (two grams per 1000 ml distilled water) and pre-chilling (seven ...
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Randomized factorial design was implemented in four replications to determine optimal methods of Ajwain (Carum capticum) standard seeds germination. Ajwain seeds were placed top and between paper and sand exposed to two potassium nitrate (two grams per 1000 ml distilled water) and pre-chilling (seven days under 7-10ºC) pre-treatments which were under six temperatures (10, 15, 20, 25, 20-30, and 35ºC) in germinator for 25 days so as to germination indices were assessed. Ajwain seeds viability was estimated 75% after tetrazolium test. The highest final and normal seedling percentages were found under 15 and 20ºC in pre-chilling treatment and also 20ºC in potassium nitrate and control. No germination was observed under 35ºC in potassium nitrate and control, however, pre-chilling led to >26% germination. The higher germination potential was shown in top and between papers compared with sand. The lowest germination rate was recorded in control and potassium nitrate and the highest one in pre-chilling. The maximum of seedling weight vigor was suggested in top and between paper when seeds were put under 10, 15, and 20ºC. In order to evaluate optimal standard germination of Ajwain seeds, it was resulted that applying of two potassium nitrate and pre-chilling treatments placed top and between papers under 15 and 20ºC caused maximum of germination potential and seedling weight vigor.
Bita Oskouei; Abbas Dehshiri; Hadis Afshar; Elahe Gheysari; Ali Shayanfar
Abstract
An experiment was implemented based on completely randomized design with four replications to determine standard Flixweed (Descurainia sophia) germination test condition. Flixweed seeds were placed in germinator for 14 days under 6 temperatures 10, 15, 20, 25, 20-30, 35ºC exposed to three treatments ...
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An experiment was implemented based on completely randomized design with four replications to determine standard Flixweed (Descurainia sophia) germination test condition. Flixweed seeds were placed in germinator for 14 days under 6 temperatures 10, 15, 20, 25, 20-30, 35ºC exposed to three treatments of potassium nitrate (2 grams per 1000 ml of distilled water), pre-chilling (7-10ºC for 7 days), pre-soaking (24h in distilled water), two cellulose substrates of top paper and between paper, and sands. Then, germination indexes were assessed. After comparison of three mentioned treatments (potassium nitrate, pre-chilling, and pre-soaking) with control it was demonstrated that the highest germination rate belonged to pre-chilling in 15ºC.The lowest amount of germination indices and the highest percentage of dead seeds were found in 25 and 35ºC. The best treatment to improve germination and seedling vigor was applying of potassium nitrate and optimal temperatures to reach maximum of germination was 10 and 15ºC. Higher germination was achieved when top and between paper substrate were used than sand. In total, it was suggested that it would be better for potassium nitrate to be under 10 and 15ºC when top and between paper and sand were applied, respectively to optimal condition for standard germination determination was achieved, because the highest percentage of final germination, normal seedlings, and seedling weight vigor, and also the lowest percentage of dead seeds were observed.
Bita Oskouei; Leila Sadeghi; Fatemeh Doorooshi
Abstract
In the national standard for canola seed production, wild mustard is considered an illegal weed. Although canola and wild mustard can be distinguished by the morphological traits, it is not possible to distinguish the seeds of two species, especially the coated seeds. The present study was conducted ...
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In the national standard for canola seed production, wild mustard is considered an illegal weed. Although canola and wild mustard can be distinguished by the morphological traits, it is not possible to distinguish the seeds of two species, especially the coated seeds. The present study was conducted to optimize the identification and detection of wild mustard seeds in rapeseed lots by morphological, chemical and molecular methods. The other seed count test was performed according to the rules of international seed test in rapeseed sampling. Chemical test design was performed under KOH treatment. The results of the chemical test were presented in five different status. Three specific markers of DA, DC and 5S rDNA were used, respectively. The Cruc marker is thought to be an internal control. Multiple polymerase chain reaction show amplification patterns of wild-mustard seed-specific markers with 5S rDNA amplified 190 bp that did not amplify in canola seeds. DA and DC markers in canola seeds amplified 239 and 625 bp, respectively, which did not amplify in wild mustard. According to molecular specific Profile and comparison with different status of rapeseed. Morphological limitation features and similarity of expression states of seed-related traits in wild mustard and rapeseed and factors such as seed coverage and chemical treatment restriction of wild mustard from rapeseed masses according International seed testing will not be possible, in which case DNA-based molecular tools will provide reliable results.
S. Sheidaei; Aidin Hamidi; Hossein Sadeghi; Bita Oskouei
Abstract
A factorial experiment was conducted to evaluate the biochemical changes during the soybean seed deterioration based on a completely randomized design. The treatments consisted of germination in two levels of minimum and high quality, three initial seed moisture content (low, medium and high moisture), ...
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A factorial experiment was conducted to evaluate the biochemical changes during the soybean seed deterioration based on a completely randomized design. The treatments consisted of germination in two levels of minimum and high quality, three initial seed moisture content (low, medium and high moisture), and two storage conditions were considered including the common storage in Moghan and the controlled storage condition. Indices of germination percentage, vigor and biochemical changes including protein content, soluble sugars, malondialdehyde, catalase and peroxidase enzymes were evaluated. The study of biochemical changes of deteriorated seeds during the storage showed that, with the increase of seeds’ deterioration, the soluble sugars and protein percentage decreased, as well. Moreover, seeds’ storage in poor condition caused a significant decrease in seeds’ soluble sugar and total protein content. Also, the increase of seed moisture was followed by further seed deterioration that significantly decreased the content of soluble sugars and total protein. Significant differences were observed between seeds with different initial germinations and they had significantly higher content of soluble sugars and protein percentages. Furthermore, measuring the amount of malondialdehyde and the activity of catalase and peroxidase enzymes indicated that there exists a significant correlation between the degree of seed deterioration with the level of malondialdehyde and the activity of scavenging enzymes. Overall, along with the decrease of germination and the quality of soybean seeds after the storage, the amount of lipids peroxidation increased and the seed deterioration decreased the activity of catalase and peroxidase enzymes during the storage.
Bita Oskouei; Aidin Hamidi; Saman Sheidaei; maryam divsalar; hossein sadeghi
Abstract
This experiment was done in seed and plant certification and registration institute laboratory in 2015. The treatments were corn seed produced in Karaj and Moghan , seed storing condition in two controlled and Free weather conditions stores also Disinfection and non- Disinfection. The experiment was ...
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This experiment was done in seed and plant certification and registration institute laboratory in 2015. The treatments were corn seed produced in Karaj and Moghan , seed storing condition in two controlled and Free weather conditions stores also Disinfection and non- Disinfection. The experiment was conducted as factorial based on completely randomized design in three replications. Germination percent of Karaj seeds before accelerated aging test was 92 percent and after 144 hours aging, it reduced 3 percent in controlled storage and it dropped 6 percent in Moghan conditions storage. While similar condition led to a decrease of 18 and 28 percent in Moghan seeds produced. When seeds were stored in controlled storage, indicated 17 percent higher protein content compared to storage in Moghan conditions. Catalase activity of Karaj seeds was higher than Moghan seeds (14%). Seed treatment had significant effect on peroxidase and MDA content, as peroxidase activity in treated seeds was 11 percent higher and MDA content was 8 percent lower than untreated seeds. Karaj seeds in controlled storage had the highest peroxidase activity and the lowest MDA content. So production seed conditions in Moghan was more critical in terms of temperature and humidity than Karaj so the biochemical properties of seeds have been affected. So that Moghan seeds had less storability after storing. On the other hand, seeds in Moghan store subjected to more critical temperature and humidity than controlled condition so more deteriorated.
A. Hamidi; Hossein Sadeghi; Hamidreza Gazor; Saman Sheidaei; Bita Oskouei; Hassan Mivechi; Mohammad Nouri; Shapour Alizadeh; Saber Seifamiri; Leila Zare; Azam Dashti
Abstract
This research in order to study on post-harvest process effect on 2 soybean commercial Williams and Saba (L17) in Moghan region was conducted as a 2×5, two cultivars× 5 post-harvest process stages including after harvest, after drying, after cleaning after packing and stacking and storage, ...
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This research in order to study on post-harvest process effect on 2 soybean commercial Williams and Saba (L17) in Moghan region was conducted as a 2×5, two cultivars× 5 post-harvest process stages including after harvest, after drying, after cleaning after packing and stacking and storage, factorial experiment based on completely randomized design by 3 replications. Studied traits were normal seedlings percent in standard germination test and after accelerated ageing test, seedling weight vigour index after standard germination test, broken seeds percent, cracked coat seeds percent and electrical conductivity. Results revealed Williams cultivar had more normal seedlings percent after accelerated ageing test and seedling weight vigour index after standard germination test and less cracked coat seeds percent. Also the least normal seedlings percent in standard germination test, normal seedlings percent after accelerated ageing test and seedling weight vigour index after standard germination test and the most broken seeds percent, cracked coat seeds percent and electrical conductivity investigated. Based on this research results Williams cultivar seeds had more germination ability and vigour preservation potential during post-harvest process stages than Saba (L17) cultivar. Also germination ability and vigour decrease after drying and storage stages revealed seed drying unsuitability.
