Bita Oskouei; Abbas Dehshiri; Hadis Afshar; Elahe Gheysari; Ali Shayanfar
Abstract
An experiment was implemented based on completely randomized design with four replications to determine standard Flixweed (Descurainia sophia) germination test condition. Flixweed seeds were placed in germinator for 14 days under 6 temperatures 10, 15, 20, 25, 20-30, 35ºC exposed to three treatments ...
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An experiment was implemented based on completely randomized design with four replications to determine standard Flixweed (Descurainia sophia) germination test condition. Flixweed seeds were placed in germinator for 14 days under 6 temperatures 10, 15, 20, 25, 20-30, 35ºC exposed to three treatments of potassium nitrate (2 grams per 1000 ml of distilled water), pre-chilling (7-10ºC for 7 days), pre-soaking (24h in distilled water), two cellulose substrates of top paper and between paper, and sands. Then, germination indexes were assessed. After comparison of three mentioned treatments (potassium nitrate, pre-chilling, and pre-soaking) with control it was demonstrated that the highest germination rate belonged to pre-chilling in 15ºC.The lowest amount of germination indices and the highest percentage of dead seeds were found in 25 and 35ºC. The best treatment to improve germination and seedling vigor was applying of potassium nitrate and optimal temperatures to reach maximum of germination was 10 and 15ºC. Higher germination was achieved when top and between paper substrate were used than sand. In total, it was suggested that it would be better for potassium nitrate to be under 10 and 15ºC when top and between paper and sand were applied, respectively to optimal condition for standard germination determination was achieved, because the highest percentage of final germination, normal seedlings, and seedling weight vigor, and also the lowest percentage of dead seeds were observed.
Mahrokh Bolandi; Parisa Sheikhzadeh; Saeed Khomari; Nasser Zare; Jaber Sharifi
Abstract
To investigate the effect of the maternal plant's growth environment on qualitative characteristics and germination of borage seeds after storage, an experiment was carried out in a randomized complete block design with three replications at the University of Mohaghegh Ardabili. The treatments included ...
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To investigate the effect of the maternal plant's growth environment on qualitative characteristics and germination of borage seeds after storage, an experiment was carried out in a randomized complete block design with three replications at the University of Mohaghegh Ardabili. The treatments included seeds resulting from maternal plants sprayed with humic acid (0.5 g L-1), chitosan (1 g L-1), and the combination of humic acid and chitosan (0.5 g L-1 + 1 g L-1), salicylic acid. (4 mM), ascorbic acid (0.85 mM), potassium nitrate (20 mM) and control.The results showed that under non-storage conditions, the seeds that resulted from the maternal plants treated with bio-stimulants and growth regulators exhibited higher seed germination and seedling growth than those from the control plants. In this condition, foliar application of the maternal plants with humic acid+chitosan had the highest effect on the seed germination and seedling growth characteristics by increasing the germination percentage and rate, vigor index, seedlings dry weight and length. Although five years of dry seed storage reduced the seed viability and vigor, foliar application of maternal plants reduced the negative effects of storage on germination and enhanced the seed germination percentage and seedlings length, and dry weight by increasing the seed viability and seed vigor index. In general, among the foliar spraying treatments of the maternal plants, the combined foliar application of humic acid+chitosan, maybe due to the synergistic effects, can be considered the best treatment to improve the borage seed longevity, germination characteristics, and seedling growth.
H. Zaremanesh; H.R. Eisvand; N. Akbari; A. Ismaili; M. Feizian
Abstract
In order to reduce the effects of salinity on germination and growth of Khuzestani savory by humic acid, two experiments were performed. The first experiment was done by priming the seeds with zero concentrations, 20, 40 and 60 mg / l of humic acid (H1, H2, H3 and H4, respectively) and germinating them ...
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In order to reduce the effects of salinity on germination and growth of Khuzestani savory by humic acid, two experiments were performed. The first experiment was done by priming the seeds with zero concentrations, 20, 40 and 60 mg / l of humic acid (H1, H2, H3 and H4, respectively) and germinating them in zero salinity (distilled water), 50, 25, 75 and 100 mM أNaCl (S1, S2, S3, S4 and S5, respectively) in petri dishes. In the second experiment in the greenhouse, humic acid at zero, 10, 20, 30 and 40 mg / kg soil (H1, H2, H3, H4 and H5, respectively) and salinity stress including zero, 50, 25, 75 and 100 mM sodium chloride (S1, S2, S3, S4 and S5, respectively). The results of the first experiment showed that germination percentage and rate were decreased by increasing in salinity, While, germination rate and percentage were improved by humic acid priming. Salinity also decreased the growth characteristics in pot experiment. While humic acid led to the improvement of these growth traits. With increasing salinity, electrolyte leakage increased, but it was reduced by humic acid. The positive results of using humic acid in reducing the harmful effects of salinity, in addition to laboratory (seed priming) were also proven in pots (soil application). Therefore, in general, according to the positive results of this study on improving the germination and growth indices of Khuzestani savory plant, the use of humic acid can be recommended to reduce the adverse effects of salinity.
Abstract
In order to investigate the effect of seed bio-inoculation on some germination indices of Cumin under drought stress a two factors experiment was laid out. Experimental factors included priming in nine levels (bio- inoculation with 4 strains of PF1, PF2, PF75 and CHA0 of Pseudomonas fluorescent, and ...
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In order to investigate the effect of seed bio-inoculation on some germination indices of Cumin under drought stress a two factors experiment was laid out. Experimental factors included priming in nine levels (bio- inoculation with 4 strains of PF1, PF2, PF75 and CHA0 of Pseudomonas fluorescent, and 4 strains T29, T36, T39 and T40 of Trichoderma harzianum and non-primed (control)) and three levels of drought stress (0, -3 and -6 bar imposed by PEG 6000). Results showed that by increasing the osmotic potential levels germination indices decreased. At all levels of stress, inoculated seeds had higher germination percentage and root length than non-inoculated seeds. In under stress level of -3 bar, highest germination percentage (86.66%) and seedling weight vigor index (11.93) was obtained from T36 strain that; this treatment was able increase germination by more than 35% than non-inoculated seed. Under -6 bar, the highest (76.68%) and lowest (55%) germination percentage was related to T29 that has no significant difference with T36 (75.83%) and T39 (75.83%) and the lowest amount was related to non-primed treatment (55%). Also at all stress level, the highest length seedling vigor index was related to CHA0 strain. The highest weight seedling vigor index was related to T36 strain (6.4, 5.49 and 4.7 respectively in 0, -3 and -6 abr) and the lowest (4.89) was related to non-inoculated (control) seeds. According to the results, T36 can be suggested as a suitable pretreatment in moderation of the negative effects of osmotic potential on germination of cumin seeds.
Sayyed Saeed Moosavi; Payman Nosrati; Mohammad Reza Abdollahi
Abstract
Datura is an important medicinal plant for production of various alkaloids. Seed dormancy and the problem of germination is the most important barrier to produce this plant. In order to solve the germination problem in this important medicinal plant, the present study was carried out as a factorial experiment ...
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Datura is an important medicinal plant for production of various alkaloids. Seed dormancy and the problem of germination is the most important barrier to produce this plant. In order to solve the germination problem in this important medicinal plant, the present study was carried out as a factorial experiment with three factors based on completely randomized design with three replications. The first factor consisted of two datura ecotypes (Hamadan and Esfahan), the second factor included three levels of physical treatments of breaking dormancy (no scratching; scratch with sandpaper and wet bathing of bain marie bath for 7 days at 60 °C; scratch with sandpaper and dry chilling for 7 days at 4 °C), and the third factor included five levels of chemical treatments of breaking dormancy (no application of chemical treatments [putting the seeds on distil water from the beginning of the experiment to the end of the experiment]; NaOH 20% for 90 minutes; NaOH 20% for 90 minutes + gibberellin 500 ppm for 48 hours; NaOH 20% for 90 minutes + potassium nitrate 300 ppm [putting the seeds in potassium nitrate solution from the beginning of the experiment to the end of the experiment]; NaOH 20% for 90 minutes + gibberellin 500 ppm for 48 hours + potassium nitrate 300 ppm). Interaction of ecotypes and seed dormancy breaking treatments was significant for most traits except; number of leaves per plant, root fresh weight, shoot length and stem fresh weight. The results showed that the chemical treatment of NaOH 20% + gibberellin 500 ppm, was the best dormancy braking treatment for the fresh harvested seeds of datura. Therefore, using this treatment is recommended to break seed dormancy more than 70 percent, without significant damage on the seeds. In general, it was found that the chemical treatments were more effective than physical treatment on breaking the dormancy of datura seed and the main cause of breaking seed dormancy and stimulation of vegetative growth of seedlings obtained was the combination of NaOH with gibberellin.
Parisa Sheikhzadeh; Hamid Shafiyar; Saeed Khomari; Hamid Reza Mohammad Doost
Abstract
Borage is one of the important medicinal plants with unequal and poor seed germination as a result of deterioration during storage. With attention to abundance of salty water and soils in country, this research was contacted to study of the effect of priming and deterioration of seed on germination, ...
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Borage is one of the important medicinal plants with unequal and poor seed germination as a result of deterioration during storage. With attention to abundance of salty water and soils in country, this research was contacted to study of the effect of priming and deterioration of seed on germination, growth and biochemistry characteristic of borage under salinity stress. The experimental treatments were salinity stress, seed priming and deterioration duration (0, 8 and 14 hours). The results showed that percentage and rate of germination, seedling dry weight and length and weight were decreased with increasing of salinity stress levels and duration of deterioration. Seedlings dry weight loss was about 75% to 78% under salinity stress. Activity of peroxidase and catalase enzymes and proline content in seedling were increased under salinity stress, and this increase in seedlings from primed seeds was significantly higher than those of control seeds. Seed priming significantly increased the seed germination, growth and prolin content of seedling in both salinity stress and deterioration conditions. Seed priming caused a 1.6 and 2.5- fold increase in the catalase activity of seedlings derived from undeteriorated and deteriorated (14 hours) seeds, respectively. Generally, seed priming with ascorbic acid was reduced the negative effects of salinity stress and deterioration through increasing seed vigor and improving the biochemical properties of seedlings, and led to improve the seed germination and seedling growth under favorable and unfavorable conditions.
Hamidreza Naseri; Seyed Alireza Hosseini; Nateq Lashkari Sanami
Abstract
Water deficiency is one of the important problems in arid and semi-arid regions. Thus, in germination and growing stages selection of resistant medicinal plant species is very important. In this investigation in order to seed dormancy breakage of Dorema ammoniacum seeds, wet cooling treatment was assessed ...
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Water deficiency is one of the important problems in arid and semi-arid regions. Thus, in germination and growing stages selection of resistant medicinal plant species is very important. In this investigation in order to seed dormancy breakage of Dorema ammoniacum seeds, wet cooling treatment was assessed first. After selection of the best cooling treatment (5 °C), treated seeds were placed in germinating medium with drought stress; at a period of 20 days at 15 and 20 °C. Drought stress trial was conducted at 5 levels as factorial layout based on a completely randomized design including control, -4, -8, -12, and -16 bar treatments. PEG was used to make drought stress. Results showed germination rate and percentage, radicle length, and stem length were significantly (p < 0/01) decreased by increasing drought stress. The best range for germination in conditions of without stress was to -12 bar water potential. It appears that stem length was more vulnerable than other characters. In addition, 20 °C was the most appropriate temperature for germination under drought treatment.
A. Avishi; H. Feizi; M. Dashti
Abstract
To evaluate seed vigor of marigold affected as organic, biological, nano and chemical fertilizers on mother plant, a study was conducted in Research Center and Education of Agricultural and Natural Resources of Khorasan Razavi province. Plants were under the impact of 10 fertilizer treatments contains ...
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To evaluate seed vigor of marigold affected as organic, biological, nano and chemical fertilizers on mother plant, a study was conducted in Research Center and Education of Agricultural and Natural Resources of Khorasan Razavi province. Plants were under the impact of 10 fertilizer treatments contains Compost (15 ton/ha), Vermicompost (5 ton/ha), cow manure (15 ton/ha), Compost with Pseudomonas putida, Vermi compost with Pseudomonas putida, Nanobiologic fertilizer (1.5 L/ha), Nano chalate ZFM (2 kg/1000 L), Nano composit Bioorganic (200 kg/ha), Chemical fertilizer (150N,50P,50K) and control. The experiment was conducted in a completely randomized design with 10 treatment with three replications. . Then traits such as percentage and speed of germination, mean germination time, seedling length, seed vigor index and seedling dry weight were measured. The results showed that fertilizer treatments had significant effects on germination percentage, germination rate, mean germination time, seedling length and vigor index of Calendula officinalis seeds but had not on seedling dry weight. The highest seed germination was found in compost treatment that was 94% (24.5% increment) and the lowest was seen in control treatment (76%). The highest germination rate was seen in compost treatment (6.05) and the lowest was in control (2.54). Application of compost in comparison to control showed 46.06% reduction in mean germination time. Also using compost had the greatest germination rate in compared to control (more than 138%). Overall compost treatment showed the best performance on the seed and seedling traits of marigold.
Volume 4, Issue 2 , December 2015, , Pages 81-96
Abstract
In order to investigate the effect of organic fertilizers on yield and seed quality criteria of Calendula officinalisL., seeds of a field experiment under application of two levels of cattle manure (without and with 20 t ha-1) and four levels of bacterial inoculant (containing N-fixing bacteria, P solubilizerbacteria, ...
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In order to investigate the effect of organic fertilizers on yield and seed quality criteria of Calendula officinalisL., seeds of a field experiment under application of two levels of cattle manure (without and with 20 t ha-1) and four levels of bacterial inoculant (containing N-fixing bacteria, P solubilizerbacteria, mixture of bacteria and control) were evaluated for seed quality criteria in a factorial experiment based on completely randomized design with four replications in Medicinal Plant Laboratory of Department of Horticultural Sciences and Landscape, University of Tehran in 2010 and 2011. Then, 1000 seed weight, seed yield and dimension and seed germination quality criteria such as seed germination percentage, rate and uniformity, time to 95% germination, seedling weight and length and seed vigour index were investigated. Results indicated that interaction effect of manure and bacterial inoculant caused significantly the highest 1000 seed weight and seed yield in both years. Nearly, all criteria related to seed dimension affected by cow manure and also bacterial inoculants in both years. Also, application of cow manure significantly affected seed germination percentage, seedling length and seed vigour index (both years) and inoculation of seed by plant growth promotion bacteria showed significant effects on seed germination percentage, seedling length and seed vigour index (both years) and seed germination uniformity, time to 95% germination and seedling weight (first year). In general, using cattle manure and also plant growth promotion bacteria resulted in the highest amount of all measured criteria compared to non application of manure and bacterial inoculants.
