Document Type : Original Article

Authors

Abstract

Myrtus communis (Myrtle) is an important medicinal plant in traditional medicine and cosmetic industry. This plant grows as evergreen shrub at slope regions and prevents soil erosion. Its seeds have elaisosomes, placed in micropile zone after fertilization. Due to dormancy, its rate and percentage of germination is low. This experiment was conducted in Payam nor university of Boshrouyeh, in order to study breaking of seed dormancy and germination of this plant. Seeds of M. communis exposed to different mechanical and chemical treatments including mechanical scarification with sand paper, sulfuric acid 90%, alcohol 96%, potassium nitrate acid 6% and H2O2 2%, each 10 minutes and untreated seeds as control. The results showed significant differences (P<1%) for all traits, including rate and percentage of germination, length of radicle and plumule, seed growth potential and germination energy. Mechanical scarification and sulfuric acid increased percentage of germination significantly (P<5%) in several traits except for germination index. The root-shoot ratio was more in all treatments than control. We concluded the type of seed dormancy is primary and inducible type that related to physical characters of seed coat.

Keywords

 
Abdul-baki, A. A., and J. D.Anderson. 1970.  Viability and leaching of sugars from germinating barely. Crop Sci. 10: 31-34.
Akhondi, M., K.  Makarian, and S. Shabani. 2011. Morphological study on pharmaceutical characters of Myrtus communis L. The first conf. of pharmaceutical plants.  Agricultural Univ. Mazandaran.
Bewley, J.D. 1997. Seed Germination and Dormancy. The Plant Cell.  9:1055-1066.
Ciccarelli D., A.C. Andreucci, A.M. Pagni., and F. Garbari. 2004. The role of the elaiosome in the germination of seed of Myrtus communis L. (Myrtaceae) seeds. Atti Soc. Tosc. Sci. Nat., 111:143-146.
Fazli, M., M. Akbarinia, M. Tabari, and H. Yousefzade. 2012. Effects of sulfuric acid on some morphological and physiological characters of Gleditshia caspica Desf. The second conferences of seed technology. Islamic Azad Univ., Mashhad.
Finkelstein, R., W. Reeves, T. Ariizumi, and C. Steber. 2008. Molecular Aspects of Seed Dormancy. Annu. Rev. Plant Biol. 59:387-415.
Gupta,V. 2003. Seed germination and dormancy breaking techniques for indigenous medicinal and aromatic plants. J. Medic. & Aromatic Plants Sci.  25: 402-407.
Hakim, M.A., A.S Juraimi., M. Begum., M. M. Hanafi, M.R. Ismail, and A. Selamat. 2010. Effect of salt stress on germination and early seedling growth of rice (Oryza sativa L.). Afr. J. Biotechnol. 9(13):1911-1918.
Kulkarni, M.G., R.A. Street, and Staden J.V. 2007. Germination and seedling growth requirements for propagation of Diosscorea dregeana (Kunth) Dur. and Schinz-A tuberous medicinal plant. S. Afr. J. Bot. 33: 131-137.
Makkizade, M., R. Farhoudi, H.A. Naghdibadi, and A. Mahdizade. 2006. Determination of the best treatment for increasing germination of  Rubia tinctorum L.,  Echinacea angustifolia D.C. and Myrtus communis L. Aromatic and pharmaceutical plants of Iran. 22(2): 105-116.
Panwar, P. and S.D. Bhardwaj. 2005. Handbook of practical forestry. Agrobios (INDIA).
Razegi, F., and R. Tavakol-Afshari. 2010. Study of drought stress on acidic and basic phosphatases present in embryonic axis at early stage of germination of wheat. Agric. Sci. 241(2): 385-393.
Roohi, A., M. Tajbakhsh, A. Bernosi, M.R. Saeedi, and P. Nikzad. 2011. Study on effects of different pre-treatments on seed germination and seedlings of Cicer arietinum L. Agron. 90
Sarmadnia, G. 1375. Seed technology. Jihad Univ. Publ., Mashhad.
Sartavi, K., and F. Gholamian. 2004. Medicinal plants of Bushehr province. Iranian J. Medic. & Aromatic Plants Res.  20 (2): 213-227.
Shanmugavalli, M., P.R. Renganayaki, and C. Menaka. 2007. Seed dormancy and germination improvement treatments in fodder sorghum. SAT J. | ejournal.icrisat.org. [Online] Available at http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.527.9695 &rep=rep1&type=pdf.
Tang. D. S., M. Hamayun, Y. Ko, Y. Zhang., S. Kang, and I. Lee. 2008. Role of red light, temperature, stratification and nitrogen in breaking seed dormancy of Chenopodium album L. J. Crop Sci. Biotechnol., 11(3): 199-204.
Tavili, A., and M. Saberi. 1388. Study on effects of pre-treatments on seed germination and growth of seedlings of Artemisia Sieberi Boiss. Natl. resources of Iran. 62(4): 515-525.
Varier, A., K. Vari, and M. Dadlani. 2010. The subcellular basis of seed priming. Curr. Sci. 99 (4): 25-32.
Verma, S.S., U. Verma, and R.P.S. Tomer. 2003. Studies on seed quality parameters in deterioration seeds in Brassica (Brassica campestris). Seed Sci. and technol. 31, 389-398.
Zargari, A. 1995. Pharmaceutical plants. vol. 2. Tehran. Univ. Publ.