Effect of desiccation tolerance on seed germination and vigor of hybrid maize (Zea mays L.)

Document Type : Original Article

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Abstract

In order to study the effect of desiccation tolerance on seed germination and vigor of hybrid maize, a field experiment was conducted using five sowing dates in three replications. Experiments were conducted in a completely randomized design in Karaj, Iran. Sampling from female rows (B73 Inbred line) was started right after pollination in a weekly scale. The harvested hybrid seeds were dried in open air conditions. Changes in seed growth and development were evaluated from 20 days after flowering and laboratory tests were performed on fresh and dried seed samples of each developmental stages. The results showed that the effects of sowing dates on normal seedling percentage, mean germination time) and seedling length was significant. The 4th sowing date had the highest normal seedling and seed vigor. Seed filling rate (3.7-9.2 mg/gram) and moisture content reduction (0.63 -1.06 percent/day) was different, depends on the sowing date and temperature of seed developmental period. It is observed that the developing seeds that dried before standard germination test, started to germinate very sooner (20-30 DAF on basis of sowing date) than fresh seeds (that start 30-50 DAF). Dried seeds reached meaningfully sooner to the highest normal seedlings percentage (30-60 DAF). When the desiccation started without hot temperature stress in third and fourth sowing dates, desiccation tolerance completed sooner. So the seeds reach to the highest percentage of normal seedling very soon, after drying. The evaluation of mean germination time and seedling length, as indicators of seed vigor showed that dried seeds reach to maximum germination rate and seedling length sooner than fresh seeds. The changes trend in these indicators was similar to changes in normal seedling percentage. It was concluded that appropriate temperature (lower than 35ºC) in desiccation period, especially at the beginning stages, results in fast extension of desiccation tolerance among the seeds and enhancing the seed quality after drying.

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References

 
Ajayi, S.A., G. Rühl and J.M. Greef. 2005. Physiological basis of quality development in relation to compositional changes in maize seed. Seed Sci. Technol. 33: 605-621.
Bewley J.D., and M.Black.1985.  Seeds: physiology of development and germination. New York: Plenum Press.
Bewley, J. D., and M. Black. 1994. Seeds: physiology of development and germination. 2ndedition. New York. Plenum Press.
Brenac, P., M.Horbowicz., S.M. Downer, A.M. Dickerman,M.E.Smith and R.L.Obendore. 1997. Raffinose accumulation related to desiccation tolerance during maize (Zea mays L.) seed development and maturation. J. Plant Physiol. 150:481-488
Daynard, T.B., J.W. Tanner and W.G. Duncan. 1971. Duration of the grain filling period and its relationship to grain yield in corn, (Zea mays L.). Crop Sci. 11:45-48
Egli, D.B. 1998. Seed biology and yield of grain crops. CAB international, Wallingford, UK.
Egli, D.B., D.M. Tekrony., J.J. Heitholt and J. Rupe. 2005. Air temperature during seed filling and soybean seed germination and vigor. Crop Sci. 45: 1329-1335.
Ellis, R.H. and E.H. Roberts. 1981. The quantification of ageing and survival in orthodox seeds. Seed Sci. Technol. 9: 377-409.
Golovina, E.A., F.A. Hoekstraand A. Van Alest.2001. The competence to acquire cellular desiccation tolerance is independent of seed morphological development. J. Exp. Bot. 358:1025-1027
Hampton, J. G., B. Boelt., M.P. Rolstonand and T.G. Chastain.2013.  Effects of elevated CO2 and temperature on seed quality. J. Agric. Sci. 151: 154–162
Hicks, S.D. R. 2004. The corn crop-frost and maturity. University of Minnesota [Online]. Available at http/www.extension.umn.edu
Huang, H., I.M.Muller and S.Q. Song.2012. Proteomics of desiccation tolerance during development and germination of maize embryos. J. Proteomics. 75: 1247 – 1262
Huang, H. and S.Q. Song. 2013. Change in desiccation tolerance of maize embryos during development and germination at different water potential PEG-6000 in relation to oxidative process. Plant Physiol. Biochem. 68:61-70
International Seed Testing Association (ISTA). 2007. ISTA handbook on moisture determination.Basserdorf, Switzerland.
International Seed Testing Association (ISTA). 2012. International rules for seed testing. Basserdorf, Switzerland.
International Seed Testing Association (ISTA). 2013. ISTA Handbook on Seedling Evaluation. 3rd Edition.Basserdorf, Switzerland.
Leprince O., and C.W. Vertucci.1995. A calorimetric study of glass transition behaviors in axes of bean with relevance to storage stability. Plant Physiol.109:1471–1481.
McDonald, M.B. and L.O. Copeland.1989. Seed science and technology laboratory manual. Iowa State Univ. Press.
McDonald, M. B.1999. Seed deterioration: physiology, repair and assessment. Seed Sci. Technol. 27:177–237.
Rahman, M.M., J.G.Hampton and M.J. Hill. 2005. The effect of time of sowing on soybean seed quality. Seed Sci. Technol. 33: 687-697.
Spears, J.F., D.M. Tekrony and D.B. Egli. 1997. Temperature during seed filling and soybean seed germination and vigor. Seed Sci. Technol. 25:233-244.
Tekrony,D.M., D.B.Egli and D.A.Wickham. 1989. Corn seed vigor effect on no-tillage field performance: II. Plant growth and grain yield. Crop Sci. 29:1528-1531.
Vertucci, C. W. and J.M. Farrant.1995. Acquisition and loss of desiccation tolerance. P.237-271.In J. Kigel and G. Galili (Eds.), Seed Development and Germination. Marcel Dekker Inc.New York.
Viera, R.D., D.M.Tekrony and D.B. Egli.1992. Effect of drought stress on soybean seed germination and vigor. J. Seed Technol. 15:12-21.

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