Behrooz Salehi-Eskandari; Farzaneh Aslani
Abstract
Echinophora platyloba is an aromatic perennial plant belonging to the family Apiaceae which is used as food seasoning and persevering in Iran and the shoots have medicinal properties. This study aimed to investigate treatments of moist chilling (0,30, 45, 70 and 90 days), gibberellic acid (GA3; 0, 500, ...
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Echinophora platyloba is an aromatic perennial plant belonging to the family Apiaceae which is used as food seasoning and persevering in Iran and the shoots have medicinal properties. This study aimed to investigate treatments of moist chilling (0,30, 45, 70 and 90 days), gibberellic acid (GA3; 0, 500, 1000 and 2000 mg/L) and scarification alone or a combination of these treatments for breaking seed dormancy of E. platyloba. The dormancy of the seeds was broken only after 90 days of moist chilling. Pretreatment GA only at the highest level (2000 mg/L) could induce seed germination (GP; 33%), the highest GP (50%) and germination index (GR; 3 seeds per day) was recorded in moist chilling treatment 45 days with GA3 treatment of 1000 mg/L. The lowest of GR was related to moist chilling treatment for 90 days and moist chilling treatment for 30 and 70 days with GA3 treatments of 500 and 2000 mg/L. The scarification treatment alone or combined with other treatments does not affect germination parameters.
Elham Harati; Seyyed Gholamreza Moosavi; Fatemeh Nakhaei; Mohamad Javad Seghatoleslami
Abstract
Seed germination is the first and most sensitive developmental stage in the plant life cycle and the key process in seedling emergence. On the other hand, one of the major barriers to optimum use of medicinal plants outside the natural habitat is limited level of seed germination and seed dormancy. Hence ...
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Seed germination is the first and most sensitive developmental stage in the plant life cycle and the key process in seedling emergence. On the other hand, one of the major barriers to optimum use of medicinal plants outside the natural habitat is limited level of seed germination and seed dormancy. Hence in order to find the best priming and temperature treatment to dormancy breaking and seed germination of medicinal plant seed Pulicaria gnaphalodes (Vent.) Boiss a factorial experiment based on the completely randomized design with four replications and two factors was conducted in Islamic Azad University, Birjand branch in 2017. The first factor of priming type is 11 levels included in potassium nitrate 2% treatment at two times of 24 and 48 hours, gibberellic acid (0, 250 and 500 mg/ l) at two times of 24 and 48 hours, and stratification (7, 14 and 21 days). The second factor was temperature at three levels (15, 20 and 25 °c). The results showed that highest germination rate and percent germination were related to stratification at 7 and 21 days at 25 °c and maximum seedling length, plumule length, radicle length, radicle/plumule length ratio, wet weight of seedling, dry weight of seedling, percent germination, germination rate, longitudinal index seed vigor, weight index seed vigor related to 21 days wet temperature and 15°c. Therefore, 21 days stratification and temperatures of 15 and 25 °c were the best priming and temperature treatments to dormancy breaking and seed germination of medicinal plant seed Pulicaria gnaphalodes.
zahra Mohaddes Ardebili; Hossein Abbaspour; Reza Tavakkol afshari; Seid Mohsen Nabavi Kalat4
Abstract
In order to study the effect of accelerated aging and priming with gibberellic acid on germination characteristics, lipids peroxidation, soluble protein, total phenol and electrical conductivity of wheat seed (Pishtaz Cultivar) an experiment in factorial laid out completely randomized design with three ...
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In order to study the effect of accelerated aging and priming with gibberellic acid on germination characteristics, lipids peroxidation, soluble protein, total phenol and electrical conductivity of wheat seed (Pishtaz Cultivar) an experiment in factorial laid out completely randomized design with three replications was conducted at Seed Technology Laboratory, Department of Agronomy, Faculty of Agriculture, Ferdowsi University, Mashhad, Iran, in 2016. Factors were included of seed aging(100% relative humidity at 40°C) in three levels [low deterioration (4 days), medium deterioration (6 days) and high deterioration (7 days)] and priming with gibberellic acid in four levels (0, 25, 50 and 100 ppm). Analysis of variance showed that the effect of seed aging, priming with gibberellic acid and interaction between two factors on all traits were significant. Based on the means comparison of the traits germination percentage, germination rate, soluble protein and total phenol decreased with increasing aging time. But, the level of malondyaldeid and electrical conductivity increased due to damage to cell membrane with increasing aging time. Investigation of interaction between two factors showed that the priming with gibberellic acid reduced the negative effects of seed aging on all traits.
Seyed Jalal Azari; Mahdi Parsa; A. Nezami; Reza Tavakkol Afshari; Jafar Nabati
Abstract
In order to determine the cardinal temperatures for seed germination of two lentil cultivars(Robat and Calposh) a factorial experimental were conducted base on completely randomized design with four replications. Germination temperatures includes 0, 5, 10, 15, 20, 25, 30 and 35°C and different priming ...
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In order to determine the cardinal temperatures for seed germination of two lentil cultivars(Robat and Calposh) a factorial experimental were conducted base on completely randomized design with four replications. Germination temperatures includes 0, 5, 10, 15, 20, 25, 30 and 35°C and different priming includes: control(non-priming), Gibberellic Acid(C19H22O6) 100 mg.l-1, Potassium Nitrate(KNO3) 500 mg.l-1, Zinc Sulfate(ZnSO4)100 mg.l-1, Dayan Seed Solution four ml.l-1 and Hydroperiming. The cumulative germination percentage and 50% germination rate were calculated and cardinal temperature diagrams of seed germination of two lentil varieties were plotted based on three regression models(Intersected line, Quadratic Plynomial and Dent- Like). The results showed that cumulative germination percentage and 50% germination rate were significantly affected by priming, cultivar, priming time, interaction of priming and cultivar, priming and time. The highest percentage of cumulative germination was obtained in interaction of Robat cultivar at 10°C. The highest germination rate of 50% was obtained in the interaction between Calposh cultivar and Gibberellic Acid. Among the models, the dent-like model had higher correlation coefficient in priming treatments. According to this model, the base temperature, lower optimum temperature, optimum upper and maximum values for Robat cultivar were 0.46, 23.82, 30.50 and 36.98, respectively, and for Calposh cultivar 1.44, 23.46, 30.30 and 36.87 °C. In generally, Robat cultivar is more cold tolerance than the Calposh cultivar and has a lower base temperature and Robat cultivar had higher vigor seed. Finally, the priming could reduce the cardinal temperatures of both cultivars and increase their germination ability at the lower temperatures.
razieh sadat jahanmir; Reza Tavakkol afshari; Kazem Postini
Abstract
Dehydration or Aging is one of the vigor reducing and seed germination limiting agents. Inappropriate storage places, especially the gene banks, can speed up the aging process in a variety of seeds. In this study, to improve the adverse effects of seed aging, hormone treatments with GA, Cytokinin, Auxin ...
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Dehydration or Aging is one of the vigor reducing and seed germination limiting agents. Inappropriate storage places, especially the gene banks, can speed up the aging process in a variety of seeds. In this study, to improve the adverse effects of seed aging, hormone treatments with GA, Cytokinin, Auxin and Salicylic acid in concentrations of 50, 100 and 150 ppm (and distilled water as a control) on the quality of the naturally and synthetic deteriorated seeds of wheat were studied in the seed laboratory of Agriculture and natural resources college, Tehran University in 2015. After hormonal treatment, germination under salt stress in three levels, 0, -4 and -8 bar were investigated. The results showed that the naturally aged seeds had a higher mean value for all the traits. Treatment with cytokinin hormone showed higher percentage and germination rate and WGI between others hormones and all the hormonal treatments were better than control treatment (distilled water) in all traits and increased salinity stress tolerance. Salinity stress could affect all traits except the Mean Germinations Time.
Nour Ali Sajedi; Bizhan ghazi nezami
Abstract
In order to seed germination induction and response of seedling growth of Primula vulgaris to different treatments of mechanical scarification and chemical pre-treatment, an experiment was carried out in completely randomized design with treatments of control, seed scarification (for 3 minute), warm ...
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In order to seed germination induction and response of seedling growth of Primula vulgaris to different treatments of mechanical scarification and chemical pre-treatment, an experiment was carried out in completely randomized design with treatments of control, seed scarification (for 3 minute), warm water 70 and 90˚C, priming with potasium nitrate at the rate of 2 and 4%, gibberellic acid at the concentration of 250 and 500 mg/L with three replicates in 2013. The results showed that treatments of seed scarification, warm water 70 and 90˚C and gibberellic acid of 250 and 500 mg/L increased the seedling emergence percentage compared to the control. The maximum of the daily emergence percentage recorded from gibberellic acid of 250 mg/L. Seed treatment with potasium nitrate of 2 and 4% decreased the mean emergence time by 61.2 and 51.7% compared with the control, respectively. The highest emergence rate coefficient equal to 0.094 and 0.092 was achieved from seed treatment with potasium nitrate of 2 and 4% .The maximum seedling length (5.53 cm), seedling dry weight (0.029 g), seedling vigor weight index (1.15) was obtained at gibberellic acid of 250 mg/L treatment. Treatments of seed scarification, warm water 70 and 90˚C, gibberellic acid at the concentration of 250 and 500 mg/L increased seedling vigor weight index compared to the control, significantly. In general, seed pre-treatment of Primula vulgaris with gibberellic acid of 250 mg/L was improved germination and seedling growth indices.
Abstract
Arctium lappa is a medicinal plant of Asteraceae. Seeds have a little germination due to seed dormancy. In order to break dormancy and increase the germination of its seeds an experiment with 28 treatments in a completely randomized design with four replications (25 seeds). Treatments were consisting ...
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Arctium lappa is a medicinal plant of Asteraceae. Seeds have a little germination due to seed dormancy. In order to break dormancy and increase the germination of its seeds an experiment with 28 treatments in a completely randomized design with four replications (25 seeds). Treatments were consisting of chemical scarification with sulfuric acid solution 98% for 10 and 20 min, hormone treatments of 24 and 48 hours of Gibberellic acid (GA3) (300 & 500 ppm), moist prechilling for 7 and 14 days at +5 °C temperature, and combined moist prechilling and hormonal treatments, hot water (70 °C, for 10 and 15 min.) humic acid (54 kg/L). Results showed that the effect of treatments on percentage and rate germination of Arctium lappa was significant (P≤%5). The maximum coleoptile length, radicle length, seedling length, germination percentage and germination ratio was related to 14 days moist prechilling treatment with GA3 (500 ppm- 48h) (98/78 %). On the basis of the results and with regard to the negative effect of H2SO4 and hot water on the consequential growth of seedlings, usage of 14 days moist prechilling accompanied with application of GA3 was appropriate.
Z. Aghababnejad; A. Abbasi Surki; P. Tahmasebi
Abstract
There are several limitations in reproduction of Fritilaria imperilais using tissue tissue culture, lamination and bulb division. In this study, we designed a factorial experiment in a randomized complete block for evaluating the effects of several treatments such as cold duration (0, 4 and 8 weeks), ...
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There are several limitations in reproduction of Fritilaria imperilais using tissue tissue culture, lamination and bulb division. In this study, we designed a factorial experiment in a randomized complete block for evaluating the effects of several treatments such as cold duration (0, 4 and 8 weeks), concentrations of GA3 (0, 250 and 500 ppm) and application time in three steps (before, during and after stratification). The results showed that the seeds were highly germinated using 8 weeks stratification compares to others. Moreover, 500ppm gibberllin before stratifications significantly increased percentage of germination, Vigor Index Ι, length of radical, plumule, seedling and seedling dry weight, however significantly decreased E50. The results also showed that increasing the GA3 from 250 to 500ppm had significantly positive effect on all the traits. This study indicates that 8 weeks stratification along with 500ppm is more likely to increase the germination index of Fritilaria imperilais.
T. Bazi; َAli Moradi
Volume 5, Issue 2 , January 2016, , Pages 143-155
Abstract
Prangos ferulacea L. is a plant from Apiaceae family which is used in medicinal and forage industries. Because of problems during germination and growth of this plant, several experiments were conducted in order to break dormancy and its germination enhancing. Seed samples were collected from their ...
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Prangos ferulacea L. is a plant from Apiaceae family which is used in medicinal and forage industries. Because of problems during germination and growth of this plant, several experiments were conducted in order to break dormancy and its germination enhancing. Seed samples were collected from their natural habitats (ridge of Vezg, Kogiluyeh and Boyerahmad province) in three dates of 7, 17 and 27 June 2012 and possibility of dormancy breaking were examined in the form of three separate experiments with three replicates. The first experiment factors consisted of three levels of collecting time (mentioned above) and GA3 in two levels (1000 and 1500 mg.l-1) which were applied along with 2 week’s moist chilling. The second experiment was consisted of collecting time at three levels along with moist chilling, that chilling was applied for four weeks. The third experiment factors were consisted of seed collection time in three levels and germination temperatures at two levels (15 and 20 ̊C). The Seeds were moist-chilled at 4°C for 8 weeks and then transferred to the incubators with the temperatures of 15°C and 20 °C. In the first experiment, germination of seeds collected in the third date and treated with GA 1500 mg.l-1 (33.6%) was higher than that of 1000 mg.l-1 (17.6%). The second study showed a significant positive effect of collecting time on seed germination of this plant, so that germination of seeds collected in the third date was almost doubled compared to the first date. The thirds experiment results showed that the temperature of 15 °C is more suitable than 20 °C for Prangos ferulacea seed germination. In all the three experiments the highest germination (33.26% in the first, 33.61 % in the second and 43.02% in the third experiments) were achieved when the seeds were collected at full maturity. Seeds germination obtained from 8 weeks (30.52%) chilling was higher than that of two (13.15%) and four weeks (27%). The overall results showed that Prangos ferulacea seeds have levels of physiological dormancy and seed maturity level at the time of collecting have significant effect on seed germination of this plant.
Volume 4, Issue 2 , December 2015, , Pages 51-65
Abstract
To study the problems of the Astragalus seed germination, a factorial experiment includes ecotype and dormancy breaking was carried out in base of a completely randomized design with 3 replications. The first factor consisted of different ecotypes Samirom, Damavand, Zanjan and the second factor was seed ...
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To study the problems of the Astragalus seed germination, a factorial experiment includes ecotype and dormancy breaking was carried out in base of a completely randomized design with 3 replications. The first factor consisted of different ecotypes Samirom, Damavand, Zanjan and the second factor was seed dormancy breaking treatments in 10 levels (mechanical scarification plus chilling time with 10, 20 and 30 days prechilling at 4 °C, mechanical scarification and gibberellic acid at a concentration of 400 and 500 ppm for 48 hours, scarification with sulfuric acid 96% for 2 and 4 min, mechanical scarification and 2% potassium nitrate for 72 h, mechanical scarification and gibberellic acid 400 ppm for 48 h with 20 days prechilling and intact seeds as control. Results showed that the ecotypes were different significantly for all traits except root length. Interaction of seed dormancy breaking treatments and ecotype were significant for all traits. Damavand ecotypewas higher than the restdue to the high viability, total germination percentage, germination rate and seedling vigor index. Among the treatments, mechanical scarification+gibberellic acid 400 ppm had more effective for germination parameters. Based on the results it could be said that seed dormancy is physical, because of seed scarification treatments they were able to absorb water and germinate.