Mohammad Reza Pahlevani; Mohammadali Alizadeh; Mohammad Nabi Ilkaee; Ali Ashraf Jafari; Seyed Esmaiel Seyedian
Abstract
Yarrow (Achillea) is one of the most important species of medicinal plants that has many applications in the pharmaceutical, cosmetic and health industries. In order to investigate the effect of seed priming on the germination characteristics of degenerate seeds,, A factorial As the form of completely ...
Read More
Yarrow (Achillea) is one of the most important species of medicinal plants that has many applications in the pharmaceutical, cosmetic and health industries. In order to investigate the effect of seed priming on the germination characteristics of degenerate seeds,, A factorial As the form of completely randomized design with 3 replications carried in the greenhouse of the Natural Resources Gene Bank of Research Institute Forests and Rangeland in 2022.. I, three yarrow species, Achillea.nobolis, A.millifolium and A.tenufolia, were artificially senesced by applying a temperature of 40 degrees Celsius and a relative humidity of 100% (for 0, 48 hours, 72 hours), seed priming at 3 levels, including hydropriming (soaking for 24 hours in distilled water), gibberellic acid, 250 and 500 ppm. The analysis of variance showed that there were main and interaction effects of species, seed priming and seed deterioration for all the traits of emergence Characteristics were significant at P≤ 1% . Comparison of the species showed , that of A. nobolis species higher values of all traits except for dry matter compare with two other species.The species of A. millifolium was ranked as second level. The highest emergence rate was obtained in non- deteriorated (hydro) seeds. Three species have high average mean values with gibberellic acid 250 ppm for all traits except the ratio of root/stem and percentage of dry matter. . The highest root length /Shoot were obtained with gibberellic acid 250 ppm, which indicates that the aged seeds inside Pots have more rooting improvement for their survival
Mahrokh Bolandi; Parisa Sheikhzadeh; Saeed Khomari; Nasser Zare; Jaber Sharifi
Abstract
To investigate the effect of the maternal plant's growth environment on qualitative characteristics and germination of borage seeds after storage, an experiment was carried out in a randomized complete block design with three replications at the University of Mohaghegh Ardabili. The treatments included ...
Read More
To investigate the effect of the maternal plant's growth environment on qualitative characteristics and germination of borage seeds after storage, an experiment was carried out in a randomized complete block design with three replications at the University of Mohaghegh Ardabili. The treatments included seeds resulting from maternal plants sprayed with humic acid (0.5 g L-1), chitosan (1 g L-1), and the combination of humic acid and chitosan (0.5 g L-1 + 1 g L-1), salicylic acid. (4 mM), ascorbic acid (0.85 mM), potassium nitrate (20 mM) and control.The results showed that under non-storage conditions, the seeds that resulted from the maternal plants treated with bio-stimulants and growth regulators exhibited higher seed germination and seedling growth than those from the control plants. In this condition, foliar application of the maternal plants with humic acid+chitosan had the highest effect on the seed germination and seedling growth characteristics by increasing the germination percentage and rate, vigor index, seedlings dry weight and length. Although five years of dry seed storage reduced the seed viability and vigor, foliar application of maternal plants reduced the negative effects of storage on germination and enhanced the seed germination percentage and seedlings length, and dry weight by increasing the seed viability and seed vigor index. In general, among the foliar spraying treatments of the maternal plants, the combined foliar application of humic acid+chitosan, maybe due to the synergistic effects, can be considered the best treatment to improve the borage seed longevity, germination characteristics, and seedling growth.
Khadijeh Sayahi; Amir Hossein Sari; A. Hamidi; Bahareh Nowruzi; Farshid Hassani
Abstract
In order to investigate the effect of cold plasma on seed germination characteristics and seedling growth of soybean commercial cultivars, an experiment in the form of a nest-factorial design with four replications was performed at the Plasma Physics Research Center of Islamic Azad University, Tehran ...
Read More
In order to investigate the effect of cold plasma on seed germination characteristics and seedling growth of soybean commercial cultivars, an experiment in the form of a nest-factorial design with four replications was performed at the Plasma Physics Research Center of Islamic Azad University, Tehran Research Sciences Branch and Seed and Plant Certification and Registration Institute (SPCRI) in 2021. Seeds were exposed to cold plasma for 30, 60, 180, 300 and 420 seconds. In general, germination percentage from 0 to 66.97%, germination rate from 0 to 32.17%, germination index from 3.61 to 60.09%, mean germination time from 4.62 to 66.66%, root length 2.87 to 56.13%, seedling length 2.70 to 78.13%, normal seedling number 0 to 30.26% and seedling dry weight 1.36 to 36.63% increased compared to control treatment. The results of our study showed that 60 seconds treatment had the most positive effect on seed germination characteristics and seedling growth of soybean.
Ali Jalilian; Samira Asgari; Saeid Jalali Honarmand; Mahmoud Khoramivafa; Aziz Moradi
Abstract
This experiment was conducted at Mehrgan Agricultural Research Station, Kermansha, Iran, in 2012. The maize parents of hybrid 704 cultivar was cultivated in a randomized complete block design with three replications. The treatments includes: 1-ammonium nitrate (1/3 before planting and 2/3 after planting) ...
Read More
This experiment was conducted at Mehrgan Agricultural Research Station, Kermansha, Iran, in 2012. The maize parents of hybrid 704 cultivar was cultivated in a randomized complete block design with three replications. The treatments includes: 1-ammonium nitrate (1/3 before planting and 2/3 after planting) 2-solupotas 3-iron chelate, 4-zinc sulfate, 5-manganes sulfate 6- boric acid, 7- Combine treatments 1 to 6 with each other. 8- Combine treatments 1 and 2 with each other. 9- Combine treatments 3 to 6 with each other. 10- Control (without spraying). The applied treatments were sprayed in three stages: 1- growth stage from 6 to 8 leaves 2- maximum leaf area index 3- after pollination. The length of plots was 7 meters and width was 4.5 meters (including 4 mother line and 2 father line) with spacing rows of 75 cm and plant spacing on the 18 cm row. The cultivation was carried out on May 1 and irrigation system was sprinkler. The treats measured was include macro and micro elements in seed, germination percentage, seedling vigor index (SVI), germination rate and Seedling growth rate (SGR) for each treatment. The results showed that foliar application did not significantly differ in the amount of nutrients in corn seed. Despite the lack of significant difference in the amount of nutrients in the seed, seedling growth and germination rate were superior in some treatments, so that the highest seedling growth was observed in potassium spraying treatment. The highest rate of germination was also due to spraying with high consumption elements.
Parisa Sheikhzadeh; Hamid Shafiyar; Saeed Khomari; Hamid Reza Mohammad Doost
Abstract
Borage is one of the important medicinal plants with unequal and poor seed germination as a result of deterioration during storage. With attention to abundance of salty water and soils in country, this research was contacted to study of the effect of priming and deterioration of seed on germination, ...
Read More
Borage is one of the important medicinal plants with unequal and poor seed germination as a result of deterioration during storage. With attention to abundance of salty water and soils in country, this research was contacted to study of the effect of priming and deterioration of seed on germination, growth and biochemistry characteristic of borage under salinity stress. The experimental treatments were salinity stress, seed priming and deterioration duration (0, 8 and 14 hours). The results showed that percentage and rate of germination, seedling dry weight and length and weight were decreased with increasing of salinity stress levels and duration of deterioration. Seedlings dry weight loss was about 75% to 78% under salinity stress. Activity of peroxidase and catalase enzymes and proline content in seedling were increased under salinity stress, and this increase in seedlings from primed seeds was significantly higher than those of control seeds. Seed priming significantly increased the seed germination, growth and prolin content of seedling in both salinity stress and deterioration conditions. Seed priming caused a 1.6 and 2.5- fold increase in the catalase activity of seedlings derived from undeteriorated and deteriorated (14 hours) seeds, respectively. Generally, seed priming with ascorbic acid was reduced the negative effects of salinity stress and deterioration through increasing seed vigor and improving the biochemical properties of seedlings, and led to improve the seed germination and seedling growth under favorable and unfavorable conditions.
Abstract
Arctium lappa is a medicinal plant of Asteraceae. Seeds have a little germination due to seed dormancy. In order to break dormancy and increase the germination of its seeds an experiment with 28 treatments in a completely randomized design with four replications (25 seeds). Treatments were consisting ...
Read More
Arctium lappa is a medicinal plant of Asteraceae. Seeds have a little germination due to seed dormancy. In order to break dormancy and increase the germination of its seeds an experiment with 28 treatments in a completely randomized design with four replications (25 seeds). Treatments were consisting of chemical scarification with sulfuric acid solution 98% for 10 and 20 min, hormone treatments of 24 and 48 hours of Gibberellic acid (GA3) (300 & 500 ppm), moist prechilling for 7 and 14 days at +5 °C temperature, and combined moist prechilling and hormonal treatments, hot water (70 °C, for 10 and 15 min.) humic acid (54 kg/L). Results showed that the effect of treatments on percentage and rate germination of Arctium lappa was significant (P≤%5). The maximum coleoptile length, radicle length, seedling length, germination percentage and germination ratio was related to 14 days moist prechilling treatment with GA3 (500 ppm- 48h) (98/78 %). On the basis of the results and with regard to the negative effect of H2SO4 and hot water on the consequential growth of seedlings, usage of 14 days moist prechilling accompanied with application of GA3 was appropriate.
narges khamadi; majid nabipor; habibolah roshanfeker; afrasyab rahnama
Abstract
One of factors in successful seed hydropriming technique is seed imbibition duration. To evaluate hydroprimed seed vigor of three wheat cultivars as affected by seed imbibition durations and also evaluation biochemical changes during hydropriming, two experiments was done as factorial arrangement with ...
Read More
One of factors in successful seed hydropriming technique is seed imbibition duration. To evaluate hydroprimed seed vigor of three wheat cultivars as affected by seed imbibition durations and also evaluation biochemical changes during hydropriming, two experiments was done as factorial arrangement with completely randomized design. In first experiment, effect of 7 hydropriming durations (0 , 6 , 8 , 10 , 12 , 14 and 16 h ) was research on germination and seedling growth of Star, Chamran and Fong cultivars. In second experiment, some biochemical traits and enzyme activities was measured in primed seeds for 6 , 8 and 10 h hydropriming durations. Results of first experiment showed that hydropriming with 8 h imbibition has the most positive effect on seed vigor of wheat cultivars. Among of cultivars, Star has the higher root length and root weight. Star and Chamran cultivars have no significant difference for germination rate and the lowest of this trait was observed in fong cultivar. Results of second experiment showed that longer hydropriming duration caused greater α amylase enzyme activity and higher contents of soluble carbohydrates and proteins, also increase content of malondialdehyde and insufficient antioxidant enzyme activities in 10 h than 8 h hydropriming duration was observed. Among cultivars the highest and lowest α amylase enzyme activity and soluble carbohydrates and proteins was observed in Star and Fong cultivars respectively. In this research correlation antioxidant enzyme activities with germination and seedling growth was positive and significant.
Davoud Kartoolinejad; Derakhshan Rahimi; Kazem Nourmohammadi; Reza Naghdi
Abstract
Current research aimed to evaluate the effect of nano priming using multi-walled carbon nanotubes on drought tolerance of Caucasian alder. This study was employed a factorial experiment in a completely randomized design with four replications. Drought stress was applied in the laboratory, using a solution ...
Read More
Current research aimed to evaluate the effect of nano priming using multi-walled carbon nanotubes on drought tolerance of Caucasian alder. This study was employed a factorial experiment in a completely randomized design with four replications. Drought stress was applied in the laboratory, using a solution of polyethylene glycol 6000 at 0, -2, -4, -6, and -8 bar on the primed seeds with concentrations of 0, 10, 30, 50 and 100 mg l-1 of carbon nanotubes. The results showed significant effect of nano priming and drought stress on germination factors such as germination rate and percentage, root fresh weight, shoot fresh weight and root to shoot fresh weight at the probability of 99%. The highest germination rate and percentage at all levels of drought stress, was related to100 mg l-1 of nano carbon treatment. The highest fresh weight of root and shoot at all levels of drought stress was related to 30 mg l-1 of nano carbon treatment. According to the results of this experiment it could be concluded that nano-priming improve seed germination characteristics of alder tree under drought stress.
L. Yari; M. Sedghi; A. Hamidi; R. Seyed Sharifi
Abstract
This study was conducted to investigate the role of water and polyamines (PAs) as priming agents on improving seed vigour and chilling tolerance with different moisture content (Mc)(14.0,16.0,18.0%) under chilling conditions in barley (Hordeum vulgare L.) c.v ...
Read More
This study was conducted to investigate the role of water and polyamines (PAs) as priming agents on improving seed vigour and chilling tolerance with different moisture content (Mc)(14.0,16.0,18.0%) under chilling conditions in barley (Hordeum vulgare L.) c.v Usef. Experiments were conducted at the seed and Plant certification and Registration Research Institute in 2013 -2014. The seeds were hand harvested at three initial moisture contents including 18, 16, and 14% wet weight basis .The seed samples then were sealed in polythene bags and stored in conditioned storage and equilibrium for 8 months. After that for seed priming, seeds were soaked in 20mg/L-1 (w/v) aerated solution of spermine (Spm), Puterscine (Put), and Spermidine (Spd) and distilled water (W) for 16h at 20±2C°. After each treatment, Seeds dried to original moisture level in forced air and sealed in polythene bags, stored in a conditioned storage again until one month. Experimental units were arranged as factorial in a completely randomized design with four replications after treat. The results indicated that Mc18% improved the germination percentage (GP) and seedling vigour compared to Mc 16 and 14%. The electrolyte leakage decreased in seeds harvested by Mc 18% when compared with Mc16 and Mc 14.0%. Also seed treatments significantly increased the seed viability. Likewise, in chilling stress condition maximum proline content and areal dry weight were obtained from Put treat, whereas the lowest electrolyte leakage was recorded for Put treat. Meanwhile, interaction between seed moisture content ×PAs treatment in cold condition significantly affected on plumule length and radicle dry weight, statistically maximum plumule length and radicle dry weight were recorded from Put ×18.0% mc. Also seed priming with water had positive effect on seedling growth after chilling stress. Generally, the effectiveness of PAs on improving chilling tolerance and increasing seedling vigor was more pronounced in Put treatment along with sample with 18.0% mc.
L. Yari; M. Sedghi; A. Hamidi; R. Seyed Sharifi
Volume 5, Issue 2 , January 2016, , Pages 37-50
Abstract
This study was conducted to investigate the role of polyamines (PAs) as priming agents on improving seed vigour with different moisture content (Mc)(14,16,18%) under storage conditions in barley (Hordeum vulgare L.) c.v Usef. Experiments were conducted at the Seed and Plant Certification ...
Read More
This study was conducted to investigate the role of polyamines (PAs) as priming agents on improving seed vigour with different moisture content (Mc)(14,16,18%) under storage conditions in barley (Hordeum vulgare L.) c.v Usef. Experiments were conducted at the Seed and Plant Certification and Registration Research Institute 1n 2012 -2013. The seeds were hand harvested at three initial moisture contents including 18, 16, 14% using wet weight basis. The seed samples then were sealed in polythene bags and stored in conditioned storages and for 8 months. After that for seed priming, seeds were soaked in 20mg/L-1 (w/v) aerated solution of Spermine (Spm), Puterscine (Put), and Spermidine (Spd) and distilled water (W) for 16h at 20±2C°. After each treatment, seeds were dried to original moisture level in forced air and sealed in polythene bags, stored in a conditioned storage again for one month. Experimental units were arranged as a completely randomized design with four replications after harvesting time and one month conditioned storage after treatment. The result indicated that 18% MC improved the germination percentage and seedling vigour compared to 16 and 14 % MC. Also after 8 month storage, 18% Mc had the highest germination percentage and seedling vigour. The electrolyte leakage decreased in seeds with 18% MC compared to 16 and 14% MC. Also seed treatments significantly increased the seed viability and germination percentage, whereas, mean germination time and electrical conductivity were reduced in all seed treatments. Meanwhile, maximum germination percentage was detected for seed Spm seed treatment and 18% MC. Generally by decreasing moisture content of seed and delay in harvesting time, seedling vigour decreased and the optimum moisture content and seed treatment were 18% and Spm, respectively.
Volume 4, Issue 1 , March 2015, , Pages 1-13
Abstract
To study the effects of drought stress and nitrogen fertilizer levels on some seed quality indices of two red kidney bean genotypes, a field experiment was carried out as a split factorial arranged in a randomized complete blocks design with three replications. The main plots were three drought stress ...
Read More
To study the effects of drought stress and nitrogen fertilizer levels on some seed quality indices of two red kidney bean genotypes, a field experiment was carried out as a split factorial arranged in a randomized complete blocks design with three replications. The main plots were three drought stress levels [irrigation after 60 (without stress), 90 (mild stress) and 120 (severe stress) mm evaporation from open pan class A] and factorial combinations of N fertilizer at four levels (0, 50, 100, and150 kg N ha-1) and two red bean genotypes (Akhtar and D81083) were assigned in sub-plots. The seeds were evaluated by standard germination test and electrical conductivity (EC) test after harvesting at full ripeness. These experiments were carried out as factorial based on a completely randomized design. Results showed that drought stress decreased normal seedling percentage, root length, shoot length, root weight, shoot weight, and seedling vigor index. Also, application of nitrogen fertilizer increased EC value, root length, shoot length and seedling length vigor index. Generally, germination indices of D81083 in non-stress conditions were better than Akhtar but, there was not significant difference between two genotypes in terms of severe drought stress level
