Parimah Shokouhi Nasab; Hamidreza Balouchi; َAli Moradi; Mohsen Movahhedi Dehnavi
Abstract
This experiment was conducted in order to investigate the effect of seed coating with biochar and activated carbon on some quinoa seed germination indicators, and it was implemented as a factorial in the form of a completely randomized design with four replications in 2019, in Yasouj University's Faculty ...
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This experiment was conducted in order to investigate the effect of seed coating with biochar and activated carbon on some quinoa seed germination indicators, and it was implemented as a factorial in the form of a completely randomized design with four replications in 2019, in Yasouj University's Faculty of Agriculture. The first factor is seed coating in four levels (no coating, coating with activated carbon, coating with biochar and coating with activated carbon + biochar) and the second factor includes salinity stress in four levels (zero, 75, 150 and 225 mM sodium chloride). The results of the interaction of salinity stress and seed coating showed that the highest content of soluble sugar (28.578 mg/g seed FW) and malondialdehyde (2.97 µmol/g seed FW) was obtained by coating with activated carbon in 150 mM salinity stress. Also, the highest amount of seed hydrogen peroxide (0.18 μmol/g seed FW) and proline (10.49 μmol/g seed FW) at the level of 225 mM sodium chloride was obtained by covering the seeds with biochar and active carbon, respectively. Covering seeds with activated carbon and biochar led to an increase in the length of root and shoot under salt stress conditions. However, under salinity stress conditions, root weight and length vigor index of seed improved more by coating seeds with activated carbon. It can be stated that seed coating can greatly reduce the harmful effects of osmotic stress on germination and biochemical traits in quinoa seedlings and improve seedling growth.
saeed saeedipour
Abstract
This experiment was conducted in order to evaluate the effects of osmo-priming on the characteristics of germination, seedling and some physiological characteristics of two cultivars of rapeseed (Brassica napus L.) under saline conditions. The seeds of two spring rape varieties were pretreated with 29.5 ...
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This experiment was conducted in order to evaluate the effects of osmo-priming on the characteristics of germination, seedling and some physiological characteristics of two cultivars of rapeseed (Brassica napus L.) under saline conditions. The seeds of two spring rape varieties were pretreated with 29.5 mM potassium nitrate (KNO3) for 12 hours at 30 degrees Celsius. In the first experiment after priming, 25 primed and unprimed seeds were placed in petri dishes with 4 levels of salinity (0, 50, 100 and 150 mM of NaCl) in a completely randomized design in four replications. The results showed that the germination percentage of primed seeds was higher than non-primed seeds.In the second experiment, 20 primed and unprimed seeds were planted in pots filled with perlite and peat at a ratio of 3:1. The pots were kept for three weeks in the greenhouse with a temperature of 26/16 degrees Celsius . The pots were watered every day with saline solutions (0, 50, 100 and 150 mM of NaCl). The results of multi-way interaction also showed that, primed seeds had higher antioxidant enzyme activity and higher content of soluble sugars and proline, at the same time, cell membrane damage in them was lower due to lower concentration of malondialdehyde compared to non-primed seeds. Under salinity conditions of 150 mM, the highest dry weight, antioxidant activity, and accumulation of soluble sugars and lower concentration of malondialdehyde were observed in Hyola 401 variety. This research showed that priming with KNO3 improves canola seed germination under stress conditions.
Abolghasem Hamidi Moghaddam
Abstract
Henna (Lawsonia inermis L.) is an ornamental and medicinal plant containing Lawson’s active substance, which is cultivated in some regions of the south and southeast of Iran. Salinity is one of the major abiotic environmental stress that affects almost all stages of plant development. Polyamines ...
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Henna (Lawsonia inermis L.) is an ornamental and medicinal plant containing Lawson’s active substance, which is cultivated in some regions of the south and southeast of Iran. Salinity is one of the major abiotic environmental stress that affects almost all stages of plant development. Polyamines such as putrescine can play important roles in plant growth and stress responses. In order to investigate the effect of putrescine and NaCl salinity stress on some germination indices, a factorial experiment was conducted with two levels of putrescine (0 and 0.75 mM) and four salinity levels (0, 50, 100 and 150 mM) based on completely randomized design with four replications in germinator for 11 days. The results showed that at control salinity level, there was no significant difference between putrescine treatments on Henna seed germination indices except the seed vigor index. The interaction between putrescine and salinity stress showed that pre-treatment with putrescine alleviated the negative effects of 50 and 100 mM salinity stress on the germination rate. However, it increased the negative effects of 150 mM salinity stress on germination percentage, radicle length, plumule length, seedling fresh weight, seed vigor index and α-amylase activity in comparison to control. The results showed that germination of Henna seeds is sensitive to salinity stress and they are only able to tolerate low levels of salinity (50 mM). According to a significant correlation between α-amylase activity with germination percentage (r= 0/98), it can be concluded that probably the change in α-amylase activity decreased the germination percentage.
Nasrin Teimoori; Mokhtar Ghobadi; Danial Kahrizi
Abstract
Drought is one of the most important stresses in reducing plant growth, especially in the seed germination stage. Oxidative stress is one of the consequences of drought in plants. To investigate the reduction of oxidative stress damage caused by drought stress by using silicon in the initial stages of ...
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Drought is one of the most important stresses in reducing plant growth, especially in the seed germination stage. Oxidative stress is one of the consequences of drought in plants. To investigate the reduction of oxidative stress damage caused by drought stress by using silicon in the initial stages of camellia seedling growth, a factorial experiment as completely randomized design was carried out in the seed laboratory of Razi University with three replications. The factors include two camelina genotypes, four levels of drought stress (0, -3, -6, -9 bar using PEG-6000) and five levels of silicon (0, 2, 4, 6 and 8 mM). According to the results, drought stress increased the activity of antioxidant enzymes including peroxidase, catalase and superoxide dismutase and the amount of malondialdehyde, but it decreased the growth characteristics of seedlings and the amount of soluble proteins. The consumption of silicon (especially by 6 and 8 mM concentrations) increased the activity of antioxidant enzymes and seedling growth characteristics. So, consumption of 8 mM silicon increased peroxidase activity by 23.2%, catalase activity by 13.4%, superoxide dismutase activity by 41%, malondialdehyde by 19.1% and protein content. Soluble was 10.8%. Therefore, the consumption of silicon 8mM by activating the antioxidant system moderated the effects of oxidative stress caused by drought stress and increased the growth characteristics of camellia seedlings.
parvin salehi; h j; L R; L F
Abstract
In this study, the effect of short-term storage conditions in one season on the characteristics of seed germination of 3 species of Nepeta was studied. A factorial experiment was conducted. Treatments included 1) Storage period (in five levels 0, 1, 2, 3 and 4 months), 2) storage temperature (at three ...
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In this study, the effect of short-term storage conditions in one season on the characteristics of seed germination of 3 species of Nepeta was studied. A factorial experiment was conducted. Treatments included 1) Storage period (in five levels 0, 1, 2, 3 and 4 months), 2) storage temperature (at three levels of the laboratory environment, cold storage +4 and -18 °C), and 3) germination treatment (at two levels of control and gibberellin 250 ppm). The results showed that the germination characteristics of different species are different at the time of harvest, So that at the time of harvesting, N. glomerulosa with 29% showed lower germination compared to two species, N. haussknechtii and N. pogonosperma, with 87 and 89% germination. Storage for 3 to 4 months increased the germination percentage of N. glomerulosa about 53% and N. haussknechtii and N. pogonosperma about 10%. It can be concluded that N. glomerulosa has primary dormancy, and by keeping it for four months, the post-harvesting demands of this species are released. While the species N. haussknechtii and N. pogonosperma did not have primary dormancy. The study of seed storage at +25, +4 and -18 °C showed that the best temperature for short-term seed storage, until the next cropping season, for N. glomerulosa subsp. Staffina is temperature +4 °C and for N. haussknechtii and N. pogonosperma is -18 °C. The germination behavior of N. haussknechtii and N. pogonosperma are very similar and is completely different from N. glomerulosa subsp. Staffina.
Farzaneh RAZAVI; Masume Asadi Aghbolaghi
Abstract
This study focused to optimize micropropagation in 11 different almond (Prunus dulcis)’s genotypes/cultivars. Main effort was to determine best explant and disinfection’s method in each genotype/cultivar. Almond’s cultivars, Mamaei (M), Shekoufeh (SH) and Fragnies (F) as well as bred ...
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This study focused to optimize micropropagation in 11 different almond (Prunus dulcis)’s genotypes/cultivars. Main effort was to determine best explant and disinfection’s method in each genotype/cultivar. Almond’s cultivars, Mamaei (M), Shekoufeh (SH) and Fragnies (F) as well as bred almond lines; A1, A2, A3, A4, A5, A7, A8 and A9 were evaluated. The effect of explant; disinfection’s methods and genotype/cultivar were analyzed using factorial experiment on completely randomized design with 3 replications for each treatment. Results indicated 3 studied factors along with their interactions can greatly affect almond’s proliferation. The explant (woody/ herbaceous shoot) showed different effects on proliferation in different almond’s genotypes/cultivars. In genotype A1, herbaceous shoot showed not suitable proliferation (30.12) compared to woody one (40.20), while in genotype A7, herbaceous shoot resulted better (40.13) than woody shoot (36.20). Interestingly, in most studied genotypes/cultivars such as A1, A3, A8, A9, SH, M, and F, woody mature shoot exposed better proliferation. Likewise, sterilization’s method had different effects on proliferation in different genotypes/cultivars. For instance, sodium hypochlorite 25 % /15 (conc. (%)/min.) showed higher proliferation in A7, A8, A9 and F, whereas, in other genotypes/cultivars, using 50 %/10 (conc. (%)/min.) was superior. Results verified almond’s genotype/cultivar and its interaction with explant and disinfection’s method, can significantly affect on proliferation as a genetic related trait. Results indicated almomd’s genotype/cultivar, method of disinfection, type of explant (herbaceous or woody shoot) and their interaction can affect significantly on almond’s proliferation, growth’s performance and rate.
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