Leyla Cheheltanan; Ali Tehranifar; Mahmoud Shoor; Seyyed Hossein Neamati; Saeed Khosravi
Abstract
Self-pollination induces a phenomenon known as inbreeding depression in heterozygous plants, which in turn leads to a reduction in seed germination and seedling growth. On the other hand, seed priming with plant hormones such as gibberellic acid is a technical approach that potentially results in the ...
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Self-pollination induces a phenomenon known as inbreeding depression in heterozygous plants, which in turn leads to a reduction in seed germination and seedling growth. On the other hand, seed priming with plant hormones such as gibberellic acid is a technical approach that potentially results in the improves of rapid and continuous seed germination and subsequent plant growth. Therefore, this research aimed to investigate the effect of different concentrations of gibberellic acid on the improvement of seed germination resulting from self-pollination in various varieties of Petunia hybrida in a factorial experiment with three replications as a completely randomized design. The treatments consisted of four levels of gibberellic acid (0, 50, 100, and 150 mg/L) and seeds obtained from self-pollination of Iranian Petunia, Tango Blue, Tritunia Pink Morn, and Tritunia White. The results indicated that seed priming with gibberellic acid, especially at a concentration of 100 mg/L, led to a significant increase in the activity of α-amylase and β-amylase by 6.56% and 1.57%, respectively. This increase resulted in 8.54% rise in soluble sugars content, providing energy that significantly enhanced germination percentage, germination speed, average time required for germination, seed vigor index, germination energy, and the fresh and dry weight of plumule and radicle. Moreover, among different varieties, seeds obtained from self-pollination of Iranian petunia demonstrated a higher germination percentage and germination speed compared to seeds from other varieties, indicating a superior capability in maintaining vigor potential, germination percentage, and germination speed.
Hadi Kalantari; Ali Izady darbandi; Ardeshir Qaderi; Seyed Ahmad Sadat noori; Mohammadreza Labbafi; Maryam Ahvazi
Abstract
The legume family has seed dormancy, which is an evolutionary adaptation that prevents seed germination in adverse ecological conditions and it is very important in preserving plant species. In order to study the effect of various mechanical, hormonal, and physical factors on the dormancy breaking of ...
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The legume family has seed dormancy, which is an evolutionary adaptation that prevents seed germination in adverse ecological conditions and it is very important in preserving plant species. In order to study the effect of various mechanical, hormonal, and physical factors on the dormancy breaking of sophora seeds, an experiment was conducted in the form of a completely randomized design in three replications. The treatments included gibberellic acid, sulfuric acid, hot water, and cold at 4 degrees Celsius in different periods. The results showed that Compared to the very low germination of untreated seeds (0-53%), the application of 75% concentration of sulfuric acid for 15 minutes, improved the germination percentage (95.5%) and also the initial growth of the seedling. The percentage of germination (93%) in the treatment having hot water was significant compared to the control sample (56%). Compared to the very low germination of untreated seeds (0-53%), sulfuric acid (73-95.5%) and soaking in hot water (52-93%) were effective in germination. According to the dormancy failure of bitter gourd seeds in the tested treatments, it can be concluded that the dormancy of bitter gourd seeds is of a mechanical type and related to the physical properties of the seed coat. treatments of hot water and l sulfuric acid can remove seed dormancy in sophora. According to the breaking sophora seeds dormancy in the tested treatments, it can be concluded that the seed dormancy is of a mechanical type and related to the physical properties of the seed coat.
Mehdi Razmkhah; Ali Moradi; Hojatollah Latifmanesh; Hamidreza Balouchi
Abstract
Camelina (Camelina sativa) is a one-year oil plant, one of the most important problems of its cultivation is the interaction of seed germination and seed establishment with drought stress. Use of herbal treatments such as rye plant extract can speed up them, especially in drought-stress conditions. Therefore, ...
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Camelina (Camelina sativa) is a one-year oil plant, one of the most important problems of its cultivation is the interaction of seed germination and seed establishment with drought stress. Use of herbal treatments such as rye plant extract can speed up them, especially in drought-stress conditions. Therefore, this research was conducted to investigate the effect of priming and priming time on germination and some biochemical traits of Camelina under drought stress in the Agriculture and Genetics Laboratory of Yasouj University from 2021 to 2022. The experiment was conducted as a factorial in a completely randomized design with 4 replications. The first factor was priming with Danko variety of rye plant root extract at 3 levels (zero, 40 and 60%), The second factor was priming times at three levels (zero, 6 and 12 hours) and the third factor included drought stress at three levels (zero, -3 and -6 times). Based on the obtained results, it was observed that drought stress affected the biochemical indicators and germination of Camelina and pre-treatment with rye extract at a concentration of 60% along with prime in 12 hours with the improvement of some biochemical indicators and germination were able to increase the tolerance of Camelina seedlings under drought stress by affecting the non-enzymatic antioxidant defense system of the plant. The results of the experiment show that using Danko variety of rye root extract with a concentration of 60% in 12 hours improves physical characteristics of the seed, helping germination in optimal and drought stress conditions.
Reza Taherdoost; Mahmood Dejam; Mehdi Madandoust; Farhad Mohajeri
Abstract
The use of sulfur (S) is of special interest in arid and semi-arid Iran since most soils are calcareous. In order to improve germination, seedling growth, and quality of Nigella sativa L. seeds obtained from mother plants grown under drought stress conditions, this investigation was performed in split ...
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The use of sulfur (S) is of special interest in arid and semi-arid Iran since most soils are calcareous. In order to improve germination, seedling growth, and quality of Nigella sativa L. seeds obtained from mother plants grown under drought stress conditions, this investigation was performed in split plots with a completely randomized block design with three replications in research farm Fasa City in 2022. The main factor was irrigation after 25, 50 and 75 percent of soil moisture depletion and the subplots were fertilizer in 8 levels including 100 % S, 50% S + thiobacillus, 50% S + mycorrhiza, 50% S+ thiobacillus+ mycorrhiza, thiobacillus, mycorrhiza, thiobacillus+ mycorrhiza and control. The results showed that in irrigation with 75 percent soil moisture depletion, the integration of 50% S+ thiobacillus+ mycorrhiza decreased the seed membrane stability by 23 percent compared to the control. In an irrigation regime with 75 percent moisture depletion, the integration of 50% S+ thiobacillus+ mycorrhiza and 50% S+ mycorrhiza caused a 31 percent increase in amylase activity in seeds endosperm about control. The highest germination percent and rate are also observed in the integration of 50% S+ thiobacillus+ mycorrhiza. Moreover in the treatment with 75 percent soil moisture depletion, the 50% S+ thiobacillus+ mycorrhiza increased the seed vigor to 46 percent. With regards to the positive effects of sulfur integration with Thiobacillus and mycorrhiza in the improvement of the quality of seeds derived from black cumin under drought stress, the use of these biological compounds should be recommended.
Faezeh Zaefarian; Mohammad Mehdi Mirzaei; Shiva Taheri
Abstract
In order to investigate the effect of pretreatment of seeds with organic fertilizers on germination and seedling indices of three cultivar of black cumin seed, a factorial experiment was conducted in the form of a completely randomized block design in four replications in 2021. The treatments of this ...
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In order to investigate the effect of pretreatment of seeds with organic fertilizers on germination and seedling indices of three cultivar of black cumin seed, a factorial experiment was conducted in the form of a completely randomized block design in four replications in 2021. The treatments of this experiment were cultivar in three levels (Iranian, Indian and Syrian) and seed pretreatment in three levels of organic fertilizers: humic acid, biozinc and control (without pretreatment). Characteristics of germination percentage and rate, germination uniformity, root, shoot and seedling length, fresh and dry weight of root, shoot and seedling, seedling tissue water content, seedling length vigour index and allometric coefficient were measured. The results showed that the studied treatments and their interaction had a significant effect on most of the measured traits. The maximum and minimum germination percentages (93.67% and 83.33%, respectively) were allocated to Iranian and Indian cultivars; while the maximum germination uniformity (256.79 hours) was obtained in the Syrian cultivar pretreated with humic acid and the minimum germination uniformity (134.40 hours) was obtained in both Iranian cultivar without pretreatment and the Iranian cultivar pretreated with biozinc. Also, the highest seedling length and seedling length vigour index were recorded in Iranian cultivars pretreated with humic acid (4.20 cm and 400.21), respectively, and the lowest ones (1.42 cm and 113.01) in Syrian cultivar pretreated with biozinc. In general, in the present study, the effect of seed pretreatment using humic acid as organic fertilizer was positive in many germination indexes.
forough firouzkoohi; Fatemeh Nasibi; Batool Keramat; Hadi Noori; Effat Ahmadi Mousavi
Abstract
Effects of dielectric barrier discharge (DBD) Helium plasma treatments on guar (Cyamopsis tetragonoloba) seed germination and seedling growth were studied. Seeds were pretreated with 0, 1/44 and 0/68 W of cold plasma for 60 s. Salt stress experiment included three salinity levels of NaCl (0, 50 and 100 ...
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Effects of dielectric barrier discharge (DBD) Helium plasma treatments on guar (Cyamopsis tetragonoloba) seed germination and seedling growth were studied. Seeds were pretreated with 0, 1/44 and 0/68 W of cold plasma for 60 s. Salt stress experiment included three salinity levels of NaCl (0, 50 and 100 mM). Results showed that plasma treatments had positive effects on seed germination and seedling (shoot length, root length, dry and fresh weight of shoot and root) , The treatment of 1.44 W of cold plasma had the most stimulating effect on the percentage of germination and growth of guar seedlings. Additionally, the application of Helium plasma treatments, considerably enhanced chlorophyll a, chlorophyll b, total chlorophyll, carotenoids, and potassium(k) content in the leaves of guar plant. Thus, cold plasma treatment can be used an ameliorative way to the enhancement germination and improve seedling growth of guar against damage caused by salt stress. Key words: dielectric barrier discharge, seed germination, guar, cold plasma, salt stress
Bita Oskouei; Leila Sadeghi; Kobra Moslemkhani; Hadis Afshar; Zahra Radmanesh
Abstract
Genetic purity, as one of the important characteristics of the quality of seed lots, has been considered in the national standards of certified seed producing countries to achieve desirable and stable characteristics of agricultural cultivars. According to the national standard, determining the other ...
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Genetic purity, as one of the important characteristics of the quality of seed lots, has been considered in the national standards of certified seed producing countries to achieve desirable and stable characteristics of agricultural cultivars. According to the national standard, determining the other speciese in the seed sample is considered as one of the important tests of seed quality. This feature is checked with an emphasis on recognizable characteristics in the seed analysis laboratory. The research has investigated the characteristics and differences in appearance and genetics of seeds off- type of chickpea seeds. The results showed that the seeds that were considered as off- type morphologically differed from the control in at least one of the measured traits. These seeds were different from the original seed in terms of color, shape seed surface roughness and seed groove, also the selected indices were similar in the first and second generation seeds. The results of the molecular test also confirmed the results of the morphological investigations. In Adel, Mansour, Arman cultivars, the samples that in morphological studies were considered as off-type, in molecular studies using microsatellite markers H3F09, H3C11 and H1A06 showed that the molecular profile was not uniform and allele diversity was greater than the main allele of cultivars. In the process of seed certification program, microsatellite markers are a suitable and complementary tool to the use of morphological markers to determine the genetic purity of chickpea seed samples.
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