نوع مقاله : مقاله پژوهشی

نویسندگان

1 استادیار، دانشگاه آزاد اسلامی واحــد اضفهان (خوراسگــان)، گروه زراعت، اصفهان، ایران

2 هیأت علمی دانشگاه آزاد اسلامی واحد اردستان، گروه زراعت، اصفهان، ایران

چکیده

کرفس کوهی با نام علمی Kelussia odoratissma Mozaff از گیاهان مرتعی و دارویی بومی ایران است که به علت برداشت غیر مجاز در معرض انقراض می‌باشد. تحقیق حاضر به بررسی تیمار مناسب برای تحریک جوانه‌زنی بذرهای این گیاه می‌پردازد. این تحقیق در قالب طرح کاملاً تصادفی بصورت آزمایش فاکتوریل در چهار تکرار انجام شد. تیمارهای آزمایش شامل سرمادهی مرطوب (دمای چهار درجه سانتی-گراد به مدت صفر، چهار و هشت هفته)، هورمون‌های جیبرلین (GA)، بنزیل آدنین (BA)، نفتالین استیک اسید (NAA)، ایندول بوتیریک اسید (IBA) (محلول های 500 پی پی ام) و ترکیبی از 4 هورمون (GA+IBA)، (GA+ NAA)، (GA+BA)، (IBA +NAA)، (IBA +BA)، (NAA+BA)، (GA+ IBA +NAA)، (GA+ IBA +BA)، (GA+NAA+BA)، (IBA +NAA+BA)، (GA+ IBA +NAA + BA) بودند. نتایج نشان داد که تیمارهای هورمونی و سرمادهی هر یک به تنهایی پارامترهای جوانه‌زنی را افزایش دادند، هر چند تیمار 8 هفته سرمادهی و اسید جیبرلیک مؤثرترین تیمارها بودند. بیشترین درصد جوانه‌زنی در GA، BA، GA+IBA+IBA و IBA بعد از 8 هفته سرمادهی در دمای 4 درجه مشاهده شد. نتایج نشان داد کرفس کوهی دارای خواب فیزیولوژیک بوده و تیمارهای با بیشترین اثر در شکستن خواب بذر GA، BA، GA+IBA+BA، BA و IBA در شرایط 8 هفته سرمادهی بودند.

کلیدواژه‌ها

Amooaghaie, R., and M. Valivand. 2011. The combined effect of gibberellic acid and long time osmopriming on seed germination and subsequent seedling growth of Klussia odoratissima Mozaff.Am. J. Bot. 10:14873–14880.
AOSA. 1983. Seed vigor testing handbook. Contribution No. 32. Association of official seed analysis. In:Valdiva CB, Sanchez-Urdaneta AB, Aguirre JR, Trejo C, Cardenas E, Villegasm A. Temperature and mechanical scarification on seed germination of maguey (Agave salmianaotto ex Salm-Dyck). Seed Sci. Technol. 34:47-56.
Bahadori, F., and A. Javanbakht. 2006. Effect of pre-treatments on seed germination and seedling growth of Bunium persicum of Semnan. IJRFPBGR (Iran). 14(3):163-169. (In Persian)
Baskin, C.C., V. Mayer, and J.M. Baskin. 1995. Two type of morpho physiological dormancy in seeds of two genera (Erythorium and Osmorhiza) with an Arcto-Tertiary distribution pattern. Am. J. Bot. 82:293-298.
Bewley, J.D., and M. Black. 1994. Seeds: Physiology of development and germination. 2nd ed. New York, Plenum Press.
Bialek, K., L. Michalczuk, and J.D. Cohen. 1992. Auxin biosynthesis during seed germination in Phaseolus vulgaris. Plant Physiol. 100:509-517.
Biddington, N.L., and T.H. Thomas. 1978. Thermo dormancy in celery seed and its removal by cytokinins and gibberllins. Physiol. Plant. 42:401-405.
Copeland, L.O., and M.B. Mc Donald. 1995. Principals of seed science and Technology. Third Edition. Chapman and Hall, New Tork, 236p.
Copeland, L.O., and M.B. Mc Donald. 2001. Principles of Seed Science and Technology. Dordrecht, the Netherlands: Kluwer Academic Publishers.
Dhoran, V.S., and S.P. Gudadhe. 2012.Effect of plant growth regulators on seed germination and seedling vigor in Asparagus sprengeri regelin. Int. Res. J. Biol. Sci. 1(7):6-10.
El-Dengawy, E.F.A. 2005. Promotion of seed germination and subsequent seedling growth of loaquat (Eriobotrya japonica) by moist-chilling and GA3 applications. Sci. Hortic. 105(3):331-342.
El-Nabawy, S., M. Abou-Rawash, A.M. El-Hamady, I. Desouky, and F. Khalil. 1980. Effect of stratification and GA3 on germination of pecan seeds and subsequent seedling growth. Ann. Agric. Sci, Ain Shams University, 25(1/2):323-338.
Etemadi, N., M. Haghighi, A. Nikbakht, and Z. Zamani. 2010. Methods to promote germination of Klussia odoratissima Mozaff., an Iranian endemic medicinal plant. Herba polonica. 56 (2):21-28.
Forozandeh Shahraki, A. 2007. Review production application methods and crop cultivation of Klussia odoratissima (Klaus). MPO (Iran). (In Persian)
Gupta, V. 2003. Seed germination and dormancy breaking techniques for indigenous medicinal and aromatic plants. J. Med. Arom. Plant Sci. 25: 402-407.
Hassan Shaykhi, A., B. Majd NAssiry, and M. Ataei kachouei. 2015. Effect of some treatments on seed dormancy, germination and antioxidant enzymes of kelussia odoratissima mozaff. Seeds. Cercetari Agronomice in Moldova. 2(162):81-90.
Iravani, M., and Z. Jaber-Alansar. 2006. Klussia odoratissima plant specious endangered, in the central Zagros region, College of Education promote the message of green, Isfahan University of Technology. (In Persian).
Karam, N.S., and M.M. Al-Salem. 2001. Breaking dormancy in Arbutus andrachna L. seeds by stratification and gibberellic acid. Seed Sci. Technol. 29:51-56.
Kato, T., M. Kobayashi, N. Sasaki, Y. Kitahara, and N. Takahashi. 1978. The coumarin hereclenol as a growth inhibitor in parsley seed. Phytochemistry. 17:158-159.
Karssen, C.M., S. Zagoreski, J. Kepczynski, and S.P.C. Groot. 1989. Key role for endogenous gibberllins in the control of seed germination. Ann. Bot. 63:71-80.
Li, M. 2000. Leung starch accumulation is associated with adventitious root formation in hypocotyls cutting of Pinus radiate. J. Plant Growth Regul. 19:423-428.
Makizade Tafti, M., and R. Farhodi. 2013. evaluation of influence dormancy breaking treatments on germination and growth of Kelussia odoratissima Mozaff. Scientific-Research Periodical of Plant and Ecosystem. 37: 53-61. (In Persian)
Mozaffarian, V. 2003. Two new genera of Iranian Umbelliferae. Res. Inst. Forest and Rangelands. 88: 88-94. (In Persian)
Nabaee, M., P. Roshandel, and A. Mohammad Khani. 2011. Effective techniques to break seed dormancy and stimulate seed germination in Rheum ribes L. IJMAP. 27(2):212-223. (In Persian)
Nabaee, M., P. Roshandel, and A. Mohammad Khani. 2013. The effects of plant growth regulators on breaking seed dormancy in Silybum marianum L. J. Cell and Tissue. 4(1):45-54. (In Persian)
Nadjafia, F., M. Bannayan, L. Tabrizi, and M. Rastgoo. 2006. Seed germination and dormancy breaking techniques for Ferula gummosa and Teucrium polium. J. Arid. Environ. 64:542-547.
Olmez, Z., Z. Yahyaoglu, and A.O. Ucler. 2004. Effects of H2SO4, KNO3 and GA3 treatments on germination of caper (Capparis ovate Desf.) seeds. Pakistan. J. Biol. Sci. 7 (6):879-882.
Otroshy, M., A. Zamani, M. Khodambashi, M. Ebrahimi, and P.C. Struik. 2010. Effect of exogenous hormones and chilling on dormancy breaking of seeds of Asafoetida (Ferula assafoetida L.). Res. J. Seed Sci. 3(4):242-248.
Razmjoo, K., A. Razzazi, N. khodaeian, and E. Askari. 2009. Breaking seed dormancy of Prangos uloptera DC., a medicinal plant of the Iran. Seed Sci. Technol. 37(3):771-775.
Roychowdhury, R., A. Mamgain, S. Ray, and J. Tah. 2012. Effect of gibberellic acid, kinetin and indole 3-acetic acid on seed germination performance of Dianthus caryophyllus (carnation). Agric. Conspectus Sci. 77(3):157-160.
SAS Institute Inc. 2006. Base SAS 9.1.3 procedures guide (2nd ed.), volumes 1, 2, 3, and 4. Author.
Sasani, S.h., R. Tavakol Afshar, K. Poostiny, and F. Sharifzadeh. 2007. Impact Assessment Moist, hormonal treatments and storage of dormancy and induce germination of seeds of Bunium persicum. Iranian J. Agric. Sci. 38(2):287-294. (In Persian)
Schimizit, N., J.H. Xia, and A.R. Kermode. 2001. Dormancy of yellow Cedar seed is terminated by GA in combination with fluridone or with osmotic priming and moist chilling. Seed Sci. Technol. 29:331-346.
Scott, S.J., R.A. Jones, and W.A. Williams. 1984. Review of data analysis methods for seed germination. Crop Sci. 24:1192–1198.
Singh, V. 1990. Influence of indole acetic acid (IAA) and indole butyric acid (IBA) on seed germination of spruce. The Indian For. 116(6):450-455.
Thomas, T.H., and J. Van Staden. 1995. Dormancy break of celery (Apiumgraveolens L.) seeds by plant derived smoke extract. Plant Growth Regul. 17:195-198.
Tipirdamaz, R., and A.N. Gomurgen. 2000. The Effects of Temperature and Gibberellic Acid on Germination of Eranthishyemalis (L.) Salisb seeds. Turk J Bot. 24:143-145.
Vandelook, F., N. Bolle, and J.A. Van Assche. 2007. Seed Dormancy and Germination of the European Chaerophyllumtemulum (Apiaceae), a Member of a Trans-Atlantic Genus. Ann. Bot. 100(2):233-239.
Walck, J.L., S.N., Hidayati, and N. Okagami. 2002. Seed germination echophysiology of Asian species Osmorhizaaristata (Apiaceae): Comparison with its North American cogenera and implications for evolution of type of dormancy. Am. J. Bot. 89:829-835.
Zafarian, Z., and S. Houshmand. 2013. Study of effects of time, quantity and application method of benzylaminopurine and gibberellic acid growth regulators on breaking seed dormancy of Kelussia odoratissima M. JCPP. 3(8):165-176.
Zhou, Z-Q., W-K. Bao, and N. Wu. 2009. Dormancy and germination in Rosa multibracteata Hemsl. & E. H. Wilson. Sci. Hortic. 119:434-441.