نوع مقاله : مقاله پژوهشی
نویسندگان
1 گروه گیاهان دارویی و تولیدات گیاهی- دانشکده کشاورزی و منابع طبیعی- دانشگاه تربت حیدریه- ایران
2 گروه تولیدات گیاهی، دانشگاه تربت حیدریه
چکیده
به‌منظور ارزیابی نقش گاما-آمینوبوتیریک اسید )گابا( و اسپرمیدین بر صفات جوانه‌زنی و شاخص‌های رشد بذر گیاه خیار تحت شرایط تنش شوری، پژوهشی به صورت فاکتوریل در قالب طرح کاملا تصادفی با سه تکرار اجرا شد. عامل اول شامل پیش‌تیمار بذر با گابا، اسپرمیدین، کاربرد توام اسپرمیدین و گابا در دو سطح 5 و 10 میلی‌مولار، آب مقطر (هیدروپرایمینگ) و عدم پیش‌تیمار بذرها به عنوان شاهد بود و عامل دوم آبیاری گیاه با سه سطح شوری با غلظت صفر (آب مقطر به عنوان شاهد)، 8/1 و 5/9 دسی‌زیمنس بر متر در نظر گرفته شد. نتایج نشان داد کاربرد پرایمینگ بذور با گابا و اسپرمیدین در برخی صفات رشدی از جمله وزن‌تر ساقه و گیاهچه، طول ساقه و وزن خشک ریشه، تاثیر مثبت داشت به گونه‌ای که تنش شوری در تمام صفات رشدی و جوانه‌زنی اختلاف آماری ایجاد کرد. بیشترین درصد جوانه‌زنی، و شاخص جوانه‌زنی در شرایط بدون تنش شوری و کاربرد اسپرمیدین 5 و گابا 10 میلی‌مولار و هیدروپرایمینگ مشاهده شد. تیمارهای هیدروپرایمینگ، اسپرمیدین و گابا تحت تیمار شوری 5/9 دسی‌زیمنس بر متر بهبود رشد معنی داری را در گیاهچه خیار نسبت به تیمار بدون شوری باعث شدند. اعمال شوری، درصد جوانه زنی را از 84% در شرایط عدم شوری به 33/17% در شوری 5/9 دسی‌زیمنس بر متر کاهش داد درحالی که افزایش شوری و کاربرد هر یک از تیمارهای پرایمینگ باعث افزایش جوانه‌زنی نسبی شد . لذا با توجه به کاربرد ارزان و راحت‌تر تیمار هیدروپاریمینگ نسبت به بقیه تیمارها، می توان هیدروپرایم را به عنوان تیمار کاربردی توصیه نمود.
کلیدواژه‌ها
Abdul-Baki, A.A., and J.D. Anderson. 1973. Vigor determination in soybean seed by multiplication. Crop Sci. 3: 630-633.
Ali, R.M., H.M. Abbas, and R.K. Kamal. 2009. The effects of treatment with polyamines on dry matter and some metabolites in salinity stressed chamomile and sweet marjoram seedlings. Plant Soil Environ, 55: 477–483.
Alsadon, A.A, M.A. Wahb-allah, and S.O. Khalil. 2006. Growth, yield and quality of three greenhouse cucumber cultivars in relation to two types of water applied at different growth stages. J. Agric. Sci. 18: 89-102.
Arbab Haghighi, H.A., F. Nejat Zade, and J. Khalili Mahale. 2017. Effect of seed priming on Moderating of salinity stress in cucumber. New Cellular Mol. Biotechnol. J. 7(27): 17-24. (In Persian)
Aslani, L., M. Mobli, and M. Solaimani. 2015. Effects of different spermidine concentrations on germination of three cucumber (Cucumis sativus) cultivars under low temperature. Iranian J. Seed Res. 2(1): 53-63. (In Persian, with English Abstract)
Balouchi, H.R., and S. Ahmadpour Dehkordi. 2013. Effect of different seed priming on germination traits in Black cumin (Nigella sativa) under salinity stress. J. Plant Prod. 20(3): 1-25.
Bertrand, A., C. Dhont, M. Bipfubusa, F.P. Chalifour, P. Drouin, and C.J. Beauchamp. 2015. Improving salt stress responses of the symbiosis in alfalfa using salt‐tolerant cultivar and rhizobial strain. Appl. Soil Ecol. 87(Supplement C):108–117.
Chattopadhayay, M.K., B.S. Tiwari, G. Chattopadhayay, A. Bose, D.N. Sengupta, and B. Ghosh. 2002. Protective role of exogenous polyamines on salinity-stressed rice (Oryza sativa) plants. Plant Physiol. 116: 192–199.
Chen, D., Q. Shao, L. Yin, A. Younis, and B. Zheng. 2019. Polyamine function in plants: Metabolism, regulation on development, and roles in abiotic stress responses. Front. Plant Sci. 9, 1945:1-13.
Cheng, B., Zh. Li, L. Liang, Y. Cao, W. Zeng, X. Zhang, X. L., Ma, Huang, G. Nie, W.,Liu, and Y. Peng. 2018. The γ-Aminobutyric Acid (GABA) Alleviates Salt Stress Damage during Seeds Germination of White Clover Associated with Na+/K+ Transportation, Dehydrins Accumulation, and Stress-Related Genes Expression in White Clover. Inter. J. Mol. Sci. 19(9): 2520.
Couee, I., I. Hummel, C. Sulmon, G. Gouesbet, and A. EL- Amrani. 2004. Involvement of polyamines in root development. Plant Cell Tissue Organ Cult. 76:1-10.
Danaee, E. and V., Abdossi. 2018. Phytochemical and Morphophysiological Responses in Basil (Ocimum basilicum L.) Plant to Application of Polyamines. J. Med. Plants. 1(69): 125-133.
Deewatthanawong, R., J. F. Nock, and C. B. Watkins. 2010. Γ-Aminobutyric acid (GABA) accumulation in four strawberry cultivars in response to elevated CO2 storage. Postharvest Biol. Technol. 57(2): 92-96.
Du, HY, Wang J, H.P. Liu, and Q.H. Yang. 2007. Effects of seed soaking with spermidine on the germination of maize seeds. J Anhui Agric. Sci. 35:11009–10.
Ellis, R.H. and E.H. Roberts. 1981. The quantification of ageing and survival in orthodox seeds. Seed Sci. Technol. 9: 377-409.
Esmailpour, N., and M. Majdam. 2010. Effect of seed hydropriming on improve of sweet sorghum germination and growth under salinity stress. J. Crop Physiol. 3: 51-59. (In Persian)
Food and Agricultural Organization. 2014. FAOSTAT [Online]. Available at https://www.fao.org/faostat. (Retrieved 9 September 2015)
Ghana, S.G., and W.F. Schillinger. 2003. Seed priming winter wheat for germination, emergence, and yield. Crop Sci. 43(6): 2135-2141.
Gonzalez-Aguilar, G.A., L. Zacarias and M.T. Lafuente. 1998. Ripening affect high temperature–induced polyamines and their changes during cold storage of hybrid fortune mandarins. J. Agric. Food Chem. 46: 3503-3508.
Groppa, M. D. and M. P., Benavides. 2008. Polyamines and abiotic stress: recent advances. Amino Acids 34: 35-45
Hajiboland, R., and N. Ebrahimi, 2011. Growth, photosynthesis and phenolics metabolism in tobacco plants under salinity and application of polyamines. J. Plant Biol. 3(7): 13-26.
Hashemi, M., H. Azarnivand., M.H. Asareh and A. Jafari, 2014. Study effect of water stress on the germination and seedling growth of three genotypes of rangeland species Agropyron podperae. J. Range. 8: 212-218.
Hosseini, H., and M. Nassiri Mahallati. 2007. The effect of seed priming in germination of lentil (Lens culinaris Medik.) genotypes. Iranian J. Agric. Res. 4:35-47. (In Persian)
Huang, Y., Ch. Lin, F. He, Zh. Li, Y. Guan, Q. Hu, and J. Hu. 2017. Exogenous spermidine improves seed germination of sweet corn via involvement in phytohormone interactions, H2O2 and relevant gene expression. BMC Plant Biol. doi.org/10.1186/s12870-016-0951-9. 17:1.
Hussein, M. M., Nadia, EL-Gereadly, H. M. and M. EL-Desuki, 2006. Role of putrescine in resistance to salinity of pea plants (Pisum sativum L.). Appl. Sci. Res. 2: 598- 604.
ISTA. 2009. International rules for seed testing. The International Seed Testing Association (ISTA) Press, Switzerland.
Kaur-Sawhney, R., A. F. Tiburcio, and A.W. Galston. 2003. Polyamines in plants: An overview. J. Cell Mol. Biol. 2: 1-12.
Khan, H.A., C.M. Ayub, M.A. Pervez, R.M. Bilal, M.A. Shahid and K. Ziaf. 2009. Effect of seed priming with NaCl on salinity tolerance of hot pepper (Capsicum annuum L.) at seedling stage. Soil Environ. 28: 81-87.
Liu, J. H., H. Inoue, and T. Moriguchi. 2008. Salt stress-induced changes in free polyamine titers and expression of genes responsible for polyamine biosynthesis of apple in vitro shoots. Environ. Exp. Bot. 62: 28-35.
Luo, H.Y., L.W. Yang, H.B. Gao, W.U. Xiao-Lei, and H.H. Liu. 2011. Physiological mechanism of GABA soaking to tomato seed germination and seedling development under NaCl stress. Acta Bot. Boreal. Occident. Sin. 31: 2235–2242.
Maguire, J.D. 1962. Speed of germination in selection and evolution for seeding vigor. Crop Sci. 2(2): 176-177.
Malekzadeh, P., J. Khara and R. Heidari. 2012. 'Effect of exogenous Gama-aminobutyric acid on physiological tolerance of wheat seedling exposed to chilling stress'. Iranian J Plant Physiol. 3 (1): 611 – 617. (In Persian)
Martin-Tanguy, J. 2001. Metabolism and function of polyamines in plants: recent development (new approaches). Plant Growth Regul. 34: 135-148.
Miller, T.R., and S.R. Chapman. 1978. Germination responses of three forage grasses to different concentration of six salts. J. Range Manage. 31(2): 123-124.
Ministry of Agriculture. 2018. Iran annual agricultural statistics. Ministry of Jihad-e-Agriculture of Iran [Online]. Available at www.maj.ir.
Najjarzadeh, S, J. Panahandeh, S. Alizadeh Salteh and F. Zaare-Nahandi. 2017. Effect of salicylic acid and putrescine on some physiological characters and essential oil of peppermint (Mentha piperita L.). Iranian J. Hortic. Sci. 47 (4): 655-667.
Nasibi, F., Kh. Manouchehri Kalantari, and N. Fazelian. 2013. The effects of spermidin and methylene blue pretreatment on some physiological responses of Matricaria recutita plants to salt stress. J. Plant Proc. Func. Iranian Soc. Plant Physiol. 1(2): 61-72.
Nasiri, S. 2009. Evaluation the effect of interaction between spermidine and salinity on Tagetes minuta L. MSc Thesis.Shahid Bahonar University of Kerman, Iran.
Nassar, A.H., K. A. El-Tarabil, and K. Sivasithamparam. 2003. Growth promotion of bean (Phaseolus vulgaris L.) by a polyamine – producing isolate of Streptomyces griseoluteus. Plant Growth Regul. 40: 97-106.
Noohpishe, Z. and K.H. Manochehri Kalantari. 2012. Effects of application of interaction of spermidine and salinity stress in pepper. Iranian J. Biol. 24: 848-857. (In Persian)
Pandey, S., S. A. Ranande, P. K. Nagar, and N. Kumar. 2000. Role of polyamines and ethylene as modulators of plant senescence. India Acad. Sci. 25: 291-299.
Pang, X. M., Z. Y. Zhang, X. P. Wen, Y. Ban, and T. Moriguchi. 2007. Polyamine, all-purpose players in response to environment stresses in plants. Plant Stress. 1: 173-88.
Pill, W.G., and T.K. Korengle. 1997. Seed priming advance the germination of Kentuckly bluegrass (Poa pratensis L.). J. Turfgrass Manage. 2: 27-43.
Raiesi, T., A. Sabouri, and H. Sabouri. 2017. Comparison between aerobic and Iranian rice (Oryza sativa L.) varieties and genotypes to drought stress tolerance at germination stage. Environ. Stresses Crop Sci. 10(1): 91-104.
Ramos-Ruiz, R., E. Poirot, and M. Flores-Mosquera. 2018. GABA, a non-protein amino acid ubiquitous in food matrices. Cogent Food Agric. 4: 1-89.
Ranjbar, M., M. Mohammadi, and L. Amjad. 2017. Lead and spermidine interact on physiological and biochemical indexes of plants Salvia officinalis L. J. Plant Proc. Func. Iranian Soc. Plant Physiol. 6(21): 103-114.
Razeghi Yadak, F., and R. Tavakkol Afshari. 2011. Effect of drought stress on seed embryo axis phosphatase activities during early stages of germination of two bread wheat (Triticum aestivum) cultivars. Iranian J. Field Crop Sci. 41(2): 385-393. (In Persian, with English Abstract)
Rho, B.J., and B.S. Kil. 1986. Influence of phytotoxin Pinus rigida on the selected plants. Korean J. Ecol. 10: 19-27.
Rhodes, D., A. Nadolska-Orczyk, and P.J. Rich. 2004. Salinity, osmolytes and compatible solutes. Pp 181-204. In Salinity: Environment-plantsmolecules. A. Läuchli and U. Lüttge (eds.). Springer Verlag, Netherlands.
Sedaghat, S. and M. Rahemi. 2011. Effect of Pre-soaking Seeds in Polyamines on Seed Germination and Seedling Growth of Pistacia vera L.cv. Ghazvini. Int. J. Nuts Related Sci. 2(3): 7-14.
Sadiq, M., G. Hassan, A.G. Khan., N. Hussain., M. Jamil, M.R. Goundal, and M. Sarfraz. 2003. Performance of cotton varieties in saline sodic soil amended with sulphuric acid and gypsum. Pakistan. J. Agric. Sci. 40: 99-105.
Shakarami, B., Gh. Dianati-Tilaki M. Tabari, and B. Behtari. 2011. The effect of priming treatments on salinity tolerance of Festuca arundinacea Schreb and Festuca ovina L. seeds during germination and early growth. Iranian J. Range. For. Plant Breed. Genet. Res. 18: 318-328. (In Persian)
Shekari, F., R. Baljani, J. Saba, K. Afsahi, and F. Shekari. 2010. Effect of seed priming with salicylic acid on growth characteristics of borage plants (Borago officinalis) seedlings. Agro. J. (Journal of New Agricultural Science). 6(18): 47-53. (In Persian with English Abstract)
Shelp, B. J., A. W. Bown, and M. D. McLean, 1999. Metabolism and functions of gamma-aminobutyric acid. Trends Plant Sci. 4(11): 446-452.
Shu, S., L.Y. Yuan., S.R. Guo., J. Sun and C.J. Liu, 2012. Effects of exogenous spermidine on photosynthesis, xanthophyll cycle and endogenous polyamines in cucumber seedlings exposed to salinity. Afr. J. Biotechnol. 11: 6064-6074
Song, H., X. Xu, H. Wang, H. Wang, and Y. Tao. 2010. Exogenous γ‐aminobutyric acid alleviates oxidative damage caused by aluminum and proton stresses on barley seedlings. J. Sci. Food Agric. 90(9): 1410-1416.
Valadiani, A.R., A. Hassanzadeh, and M. Tajbakhsh, 2006. Study on the effects of salt stress in germination and embryo growth stages of the four prolific and new cultivars of winter rapeseed (Brassica napus L.). Pajouhesh va Sazandegi. 66: 23-32. (In Persian)
Wahed, A. 2006. Exogenous and endogenous polyamines relation to growth, a-cellulose precipitation in fibres and productivity in cotton plant. Agric. Sci. 2: 139-148.
Wang, Y., W. Gu, Y. Meng, T. Xie, L. Li, J. Li, and Sh. Wei. 2017. γ-Aminobutyric Acid Imparts Partial Protection from Salt Stress Injury to Maize Seedlings by Improving Photosynthesis and Upregulating Osmoprotectants and Antioxidants. Sci. Reports.7:43609. doi: 10.1038/srep43609.
Xin SQ, Y. Gao J.M. Zhao, and X.M. Liu. 2010. Effect of seed soaking in spermidine (Spd) under salt stress on rice seed germination. North Rice. 40:23–5.
Yagmur, M., and D. Kaydan. 2008. Alleviation of osmotic stress of water and salt in germination and seedling growth of triticale with seed priming treatments. Afr. J. Biotechnol. 7: 2156-2162.
Yu, L.X., P. Zheng, S. Bhamidimarri, X.P. Liu, and D. Main. 2017. The impact of genotyping‐by‐sequencing pipelines on SNP discovery and identification of markers associated with verticillium wilt resistance in autotetraploid alfalfa (Medicago sativa L.). Front. Plant Sci. 89 (8): 1-13.
Zarei, L., Koushesh Saba, M., Vafaee, Y. and T. Javadi. 2018. Effect of Gamma-Amino-Butyric Acid foliar application on physiological characters of tomato (cv. Namib) under salinity stress. J. Plant Prod. (Sci. J. Agric.). 41(1): 16-28.
)