بررسی نقش پرایمینگ با اسپرمیدین، گاما-آمینوبوتیریک اسید و هیدرو پرایمینگ بر تعدیل اثر تنش شوری در جوانه‌زنی و شاخص‌های رشد بذر خیار (Cucumis sativus L.)رقم بیت آلفا

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه گیاهان دارویی و تولیدات گیاهی- دانشکده کشاورزی و منابع طبیعی- دانشگاه تربت حیدریه- ایران

2 گروه تولیدات گیاهی، دانشگاه تربت حیدریه

چکیده

به‌منظور ارزیابی نقش گاما-آمینوبوتیریک اسید )گابا( و اسپرمیدین بر صفات جوانه‌زنی و شاخص‌های رشد بذر گیاه خیار تحت شرایط تنش شوری، پژوهشی به صورت فاکتوریل در قالب طرح کاملا تصادفی با سه تکرار اجرا شد. عامل اول شامل پیش‌تیمار بذر با گابا، اسپرمیدین، کاربرد توام اسپرمیدین و گابا در دو سطح 5 و 10 میلی‌مولار، آب مقطر (هیدروپرایمینگ) و عدم پیش‌تیمار بذرها به عنوان شاهد بود و عامل دوم آبیاری گیاه با سه سطح شوری با غلظت صفر (آب مقطر به عنوان شاهد)، 8/1 و 5/9 دسی‌زیمنس بر متر در نظر گرفته شد. نتایج نشان داد کاربرد پرایمینگ بذور با گابا و اسپرمیدین در برخی صفات رشدی از جمله وزن‌تر ساقه و گیاه‌چه، طول ساقه و وزن خشک ریشه، تاثیر مثبت داشت به گونه‌ای که تنش شوری در تمام صفات رشدی و جوانه‌زنی اختلاف آماری ایجاد کرد. بیشترین درصد جوانه‌زنی، و شاخص جوانه‌زنی در شرایط بدون تنش شوری و کاربرد اسپرمیدین 5 و گابا 10 میلی‌مولار و هیدروپرایمینگ مشاهده شد. تیمارهای هیدروپرایمینگ، اسپرمیدین و گابا تحت تیمار شوری 5/9 دسی‌زیمنس بر متر بهبود رشد معنی داری را در گیاهچه خیار نسبت به تیمار بدون شوری باعث شدند. اعمال شوری، درصد جوانه زنی را از 84% در شرایط عدم شوری به 33/17% در شوری 5/9 دسی‌زیمنس بر متر کاهش داد درحالی که افزایش شوری و کاربرد هر یک از تیمارهای پرایمینگ باعث افزایش جوانه‌زنی نسبی شد . لذا با توجه به کاربرد ارزان و راحت‌تر تیمار هیدروپاریمینگ نسبت به بقیه تیمارها، می توان هیدروپرایم را به عنوان تیمار کاربردی توصیه نمود.

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