نوع مقاله : مقاله پژوهشی
نویسنده
گروه علوم کشاورزی، دانشگاه پیانور، تهران، ایران.
چکیده
با هدف ارزیابی اثر اسید سالیسیلیک در بهبود کارآیی بذرهای زوال یافته کدوی پوست کاغذی در شرایط تنش خشکی، آزمایشی به شکل فاکتوریل در قالب طرح کاملاً تصادفی با سه تکرار به اجرا درآمد. پیش‌تیمار غلظت‌های مختلف اسید سالیسیلیک شامل صفر (پیش تیمار شده با آب)، 1 و 5/1 میلی‌مولار در سطوح مختلف خشکی صفر، 2/0-، 4/0- و 6/0- مگاپاسکال مورد بررسی قرار گرفت. شاخص های متوسط زمان جوانه‌زنی، درصد جوانه‌زنی، طول گیاهچه، بنیه بذر، نشت الکترولیتی غشاء، قندهای محلول، پروتئین‌های محلول، مالون دی‌آلدهید، فعالیت آسکوربات پراکسیداز، سوپراکسید دیسموتاز و آنزیم‌های کاتالاز مورد ارزیابی قرار گرفتند. نتایج نشان داد پیش‌تیمار بذرها با غلظت‌های مختلف اسید سالیسیلیک، از کاهش معنی‌دار شاخص‌های جوانه‌زنی بذرهای زوال یافته کدوی پوست کاغذی تحت تنش خشکی جلوگیری نمود. به‌طوری‌که در پتانسیل 6/0- مگاپاسکال، پیش‌تیمار بذرها با 5/1 میلی‌مولار اسید سالیسیلیک درصد جوانه‌زنی، شاخص بنیه بذر، قندها و پروتئین‌های محلول را به‌ترتیب 2/80، 4/169، 6/51، 4/93 درصد و برای فعالیت آسکوربات پراکسیداز، سوپراکسید دیسموتاز و آنزیم‌های کاتالاز را به‌ترتیب 2/41، 12 و 8/11 درصد نسبت به شاهد افزایش داد. بر اساس یافته‌های تحقیق پیش‌تیمار اسید سالیسیلیک با غلظت 5/1 میلی‌مولار به منظور کاهش تنش اکسیداتیو ناشی از خشکی برای بذر های زوال یافته کدوی پوست کاغذی توصیه می‌شود.
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