نوع مقاله : مقاله پژوهشی
نویسندگان
1 گروه تولید و ژنتیک گیاهی، پردیس کشاورزی و منابع طبیعی، دانشگاه رازی.
2 گروه تولید و ژنتیک گیاهی، پردیس کشاورزی و منابع طبیعی، دانشگاه رازی، کرمانشاه، ایران.
3 استاد/گروه مهندسی تولید و ژنتیک گیاهی، دانشکده علوم و مهندسی کشاورزی، دانشکاه رازی، کرمانشاه، استان کرمانشاه، ایران.
چکیده
خشکی از تنش های بسیار مهم در کاهش رشد گیاهان به ویژه در مرحله جوانه زنی بذر است. تنش اکسیداتیو از پیامدهای خشکی در گیاهان می‌باشد. به منظور بررسی کاهش آسیب های اکسیداتیو ناشی از تنش خشکی با استفاده از سیلیکون در مراحل ابتدایی رشد گیاهچه کاملینا، آزمایشی به صورت فاکتوریل در قالب طرح کاملاً تصادفی در آزمایشگاه بذر دانشگاه رازی با سه تکرار اجرا شد. فاکتورها شامل دو ژنوتیپ کاملینا، چهار سطح خشکی (صفر، 3-، 6-، 9- بار با استفاده از پلی اتیلن گلیکول-6000) و پنج سطح سیلیکون (صفر، 2، 4، 6 و 8 میلی مولار) بودند. بر اساس نتایج، تشدید خشکی منجر به افزایش فعالیت آنزیم های آنتی‌اکسیدان شامل پراکسیداز، کاتالاز و سوپراکسید دیسموتاز و میزان مالون دی آلدئید شد، اما ویژگی های رشدی گیاهچه و میزان پروتئینهای محلول را کاهش داد. مصرف سیلیکون (به ویژه در غلظتهای شش و هشت میلی‌مولار)، فعالیت آنزیمهای آنتی اکسیدان و خصوصیات رشد گیاهچه را افزایش داد. به طوری که، مصرف سیلیکون هشت میلی‌مولار سبب افزایش فعالیت آنزیم پراکسیداز به میزان 2/23 درصد، فعالیت آنزیم کاتالاز به میزان 4/13 درصد، فعالیت آنزیم سوپراکسید‌دیسموتاز به میزان 41 درصد، میزان مالون‌دی‌آلدئید 1/19 درصد و محتوی پروتئینهای محلول به میزان 8/10 درصد گردید. بنابراین، مصرف سیلیکون در غلظت هشت میلی مولار با فعال کردن سیستم آنتی اکسیدان سبب تعدیل اثرات تنش اکسیداتیو ناشی از تنش خشکی و افزایش خصوصیات رشد گیاهچه کاملینا شد.
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