نوع مقاله : مقاله پژوهشی
نویسندگان
1 دانشگاه شاهد
2 استادیار گروه زراعت و اصلاح نباتات، دانشکده علوم کشاورزی و مرکز تحقیقات گیاهان دارویی دانشگاه شاهد تهران
چکیده
به‌منظور بررسی تأثیر بیوپرایمینگ بر شاخص‌های جوانه‌زنی، رشدی و فیزیولوژیکی گیاه کدوی تخم کاغذی تحت تنش شوری آزمایشی به‌صورت فاکتوریل در قالب طرح کاملاً تصادفی با سه تکرار اجرا گردید. تیمارهای آزمایشی شامل چهار سطح شوری (صفر، 40، 80 و 120 میلی‌مولار کلرید سدیم) و هشت سطح تیمار بیوپرایمینگ (عدم تلقیح، تلقیح با قارچ Trichoderma harzianum، تلقیح با کود زیستی ازتوبارور1، تلقیح با کود زیستی فسفاته بارور2، تلقیح با قارچ و کود زیستی ازتوبارور1، تلقیح با قارچ و کود زیستی فسفاته بارور2، تلقیح با کود زیستی ازتوبارور1 و کود زیستی فسفاته بارور2، تلقیح با تلفیقی از قارچ و هر دو کود زیستی) بودند. اثر متقابل بیوپرایمینگ و شوری بر فعالیت آنزیم‌های آنتی-اکسیدانت و شاخص‌های رشدی و جوانه‌زنی به غیر از شاخص وزنی بنیه بذر و وزن خشک گیاهچه معنی-دار بود. تیمار کود زیستی فسفاته بیشترین تأثیر مثبت بر درصد جوانه‌زنی با افزایش شوری را داشت. در تیمار تلفیق قارچ با کود زیستی فسفاته با افزایش شوری ضریب جوانه‌زنی کاهش کمتری یافت. سطوح پایین شوری بر شاخص‌های رشدی تأثیر مثبت داشت و باعث افزایش این شاخص‌ها گردید. وزن خشک با افزایش سطح شوری کاهش یافت و در تیمار قارچ بیشترین مقدار را دارا بود. با افزایش شوری میزان آنتی‌اکسیدانت‌ها افزایش یافت و استفاده از تیمارهای بیوپرایمینگ میزان این آنزیم‌ها را نسبت به تیمار شاهد بیشتر افزایش داد که نشان از مؤثر بودن این تیمارها در تعدیل اثر شوری به وسیله افزایش آنزیم می‌باشد. استفاده از میکروارگانیسم‌ها با افزایش بیشتر آنزیم‌های آنتی‌اکسیدانت نسبت به شاهد سبب کاهش اثرات منفی تنش شوری شد.
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