نوع مقاله : مقاله پژوهشی
نویسندگان
1 گروه زراعت و اصلاح نباتات / دانشکده کشاورزی و منابع طبیعی/ دانشگاه محقق اردبیلی/ اردبیل/ ایران
2 گروه آموزشی زراعت و اصلاح نباتات/ دانشکده کشاورزی و منابع طبیعی/ دانشگاه محقق اردبیلی/ اردبیل/ ایران
3 اردبیل دانشگاه محقق اردبیلی دانشکده کشاورزی و منابع طبیعی گروه زراعت و اصلاح نباتات
چکیده
به منظور بررسی اثر پرایمینگ بر جنبه‌های مختلف جوانه‌زنی بذور فرسوده کدو پوست کاغذی آزمایشی تحت تنش شوری به صورت فاکتوریل در قالب طرح کامل تصادفی با 4 تکرار در آزمایشگاه اجرا شد. تیمارها شامل سطوح مختلف فرسودگی (شاهد، 85% و 75% جوانه‌زنی شاهد) و تنش شوری (صفر، 75 و 150میلی‌مولار) و پرایمینگ (شاهد، هیدرو، جیرلین، Gr24، بنزیل‌آمینوپورین و اسپرمیدین) بود. بذرها به روش فرسودگی سریع به سطح بنیه مورد نظر رسید. صفات مورفولوژیک با استفاده از آزمون رشد گیاهچه مورد مطالعه قرار گرفتند. صفات فیزولوژیکی شامل تحرک ذخایر لیپیدی ، رنگدانه‌های فتوسنتزی ، کربوهیدرات، شدت فتوسنتز، آلفاآمیلاز و میزان یون سدیم جذب شده مورد مطالعه قرار گرفتند.
در بررسی صفات مربوط به جوانه‌زنی، تحرک ذخایر لیپیدی، کربوهیدرات، کلروفیل a، فتوسنتز و میزان سدیم، تنش شوری، فرسودگی و پرایمینگ منجر به ایجاد تغییر معنی‌دار آن‌ها نسبت به شاهد گردید. در حالی‌که در مورد وزن خشک ساقه‌چه و ریشه‌چه، کلروفیل b، و نسبت کلروفیل a/b تحت تاثیر شوری، فرسودگی و پرایمینگ تغییر معنی‌داری از نظر آماری نسبت بذور شاهد مشاهده نگردید. به طور کلی بیشترین تاثیر پرایمینگ بذور مربوط به جیبرلین در مورد صفات جوانه‌زنی و اسپرمیدین درمورد صفات فیزیولوژیک مشاهده شد.
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