نوع مقاله : مقاله پژوهشی
نویسندگان
1 گروه اگروتکنولوژی/ دانشکده کشاورزی / دانشگاه فردوسی مشهد
2 استاد
3 استادیار پژوهشکده علوم گیاهی، دانشگاه فردوسی مشهد
4 استادیار گروه بیوتکنولوژی وبه نژادی گیاهی، دانشگاه فردوسی مشهد
چکیده
این مطالعه به صورت آزمایش فاکتوریل بر پایه طرح کاملاً تصادفی با دو عامل رقم (مودنا و اوکاپی) ، سطح پیری (صفر و 24 و 72 ساعت در دمای 40 درجه سانتی‌گراد و رطوبت اشباع) و چهار تکرار برای ارزیابی سرعت و درصد جوانه‌زنی (GP)، شاخص بنیه (SV)، هدایت‌الکتریکی (EC)، فعالیت کاتالاز(CAT)، آسکوربات‌پراکسیداز (APX)، گلوتاتیون‌ردوکتاز (GR) و اس-نیتروزوتیول (SNO) در سال 1399-1398 در دانشگاه فردوسی مشهد انجام شد. تیمارهای پیری تسریع شده (AA) باعث کاهش معنی‌دار کلیه صفات (به جز EC) در بذرهای کلزا شدند. رقم مودنا در صفات جوانه‌زنی قوی‌تر و رقم اوکاپی در فعالیت‌های بیوشیمیایی برتر بود که این را می‌توان با تفاوت ژنتیکی ارقام توجیه کرد. بر اساس نتایج، در تیمارهای 72 ساعت اکاپی نسبت به شاهد، 86% GP، 88% CAT، %89 APX و 36% SNOها کاهش یافته است. هم‌چنین، همبستگی مثبتی بین SNOها و آنزیم‌های آنتی‌اکسیدانی وجود داشت؛ به عنوان نتیجه‌گیری، نیتریک اکسید (NO)به طور غیرمستقیم و به وسیله SNOها بر آنزیم‌های آنتی-اکسیدانی در بذرهای پیرشده کلزا تاثیرگذار است. پیری بذر با کاهش فعالیت آنزیم‌های آنتی‌اکسیدانی و NO و در نتیجه افزایش سرعت مرگ‌و‌میر سلول‌ها، فیزیولوژی سلول را هدف قرار می‌دهد.
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