نوع مقاله : مقاله پژوهشی
نویسندگان
1 دانشجوی دکتری دانشگاه محقق اردبیلی
2 گروه زراعت و اصلاح نباتات دانشکده کشاورزی و منابع طبیعی دانشگاه محقق اردبیلی
چکیده
به‌منظور بررسی اثر پرایمینگ و فرسودگی بر شاخص‌های رشد و فعالیت آنزیم‌های آنتی‌اکسیدان در بذر ذرت هیبرید سینگل کراس 704 آزمایشی به صورت فاکتوریل در قالب طرح کاملاً تصادفی با سه تکرار در دانشگاه محقق اردبیلی در سال 1401 اجرا شد. تیمارهای آزمایشی شامل تیمار پیری تسریع شده در سه سطح (0، 7 و 14 روز) و چهار سطح پرایمینگ (شاهد، هیدروپرایمینگ، جیبرلین (20 میلی‌گرم در لیتر) و اسید سالیسیلیک (100 میلی‌گرم در لیتر) بود. نتایج نشان داد که فرسودگی شاخص‌های رشد شامل طول ریشه‌چه، طول ساقه‌چه، طول گیاهچه، وزن تر ریشه‌چه و ساقه‌چه، وزن خشک ریشه‌چه و ساقه‌چه، شاخص طولی و وزنی بنیه بذر را کاهش داد، ولی انواع پرایمینگ به‌ویژه پرایمینگ با جیبرلین این صفت‌ها را بهبود داد. میزان فعالیت آنزیم‌های کاتالاز، سوپراکسید دیسمیوتاز و گلوتاتیون ردوکتاز در تیمار جیبرلین و فرسودگی 14 روز در مقایسه با شاهد به ترتیب در حدود 73%، 63% و 21% افزایش نشان داد. بیش‌ترین میزان فعالیت آنزیم پراکسیداز (797/1 واحد بر میلی گرم پروتئین) در پرایمینگ با جیبرلین مشاهده شد. در مجموع، کاربرد پیش‌تیمار جیبرلین موجب تقویت شاخص‌های رشد و فعالیت آنزیم‌های آنتی‌اکسیدان بذرهای ضعیف ذرت شد و می‌توان جهت افزایش بنیه بذرهای ضعیف از این تیمار استفاده کرد.
کلیدواژه‌ها
Abdoli, M. 2020. Effect of aging of seed and hydro-priming on germination characteristics and activity of some antioxidant enzymes of hybrid corn (Zea mays L.). Seed Sci. Res 7(2): 147-159. (In Persian, with English Abstract)
Abdul-Baki, A. A., and J. D. Anderson. 1973. Vigor determination in soybean by multiple criteria. J. Crop Sci. 13: 630-633.
Aebi, H. 1984. Catalase in vitro. Methods Enzymol. 105: 121-126.
Asadi Aghbolaghi, M., and F. Razavi. 2021. Improving germination and biochemical aspects in pumpkin (Cucurbita pepo) seeds deterioration under priming by salicylic acid and ascorbic acid. Iranian J. Seed Sci. Technol. 10(3): 75-88. (In Persian, with English Abstract)
Asadi Aghbolaghi, M., and M. Sedghi. 2014. The effect of osmo and hormone priming on germination and seed reserve utilization of millet seeds under drought stress. J. Stress Physiol. Biochem. 10(1): 214-221.
Baharvand, N., B. Mahdavi, and M. Dehajipour Heidarabadi. 2017. Effect of ascorbic acid on germination and activities of antioxidant enzymes of deteriorated safflower Goldasht cultivar seeds. Seed Sci. Res 4(3): 1-12. (In Persian, with English Abstract)
Bailly, C., A. Benamer, F. Cornineau, and D. Come. 2000. Antioxidant systems in sunflower (Helianthus annuus L.) seeds as affected by priming. Seed Sci. Res. 10: 35-42.
Barsa, S. M. A., I. A. Pannu, and I. Afzal. 2003. Evaluation of seedling vigor of hydro and matriprimed wheat (Triticum aestivum L.) seeds. Int. J. Agric. Biol. 5(2): 121–123.
Basra, S. M. A., L. Afzal, S. Anwar, M. Anwar-ul-haq, M. Shafq, and K. Majeed. 2006. Alleviation of salinity stress by seed invigoration techniques in wheat (Triticum aestivum L.). Seed Sci. Technol. 28: 36-46.
Canak, P., M. Mirosavljević, M. Ćirić, J. Kešelj, B. Vujošević, D. Stanisavljević, and B. Mitrović. 2016. Effect of seed priming on seed vigor and early seedling growth in maize under optimal and suboptimal temperature conditions. Selekcijai Semen. 22(1): 17-25.
Da Silva, E. A. A., P. E. Toorop, J. Nijsse, J. D. Bewley, and H. W. M. Hilhorst. 2005. Exogenous gibberellins inhibit coffee (Coffea arabica L. cv. Rubi) seed germination and cause cell death in the embryo. J. Exp. Bot. 56(413): 1029-1038.
Dadnia, M. R. 2017. Effect of Humic acid on activity of antioxidant enzymes and yield of castor bean (Ricinus commonis) under water deficit condition. J. Crop Ecophysiol 11(1): 85-98. (In Persian, with English Abstract)
Darabi, F., M. Valipour, R. Naseri, and M. Moradi. 2017. The Effects of Accelerated Aging Test on Germination and Activity of Antioxidant Enzymes of Maize (Zea mays) Hybrid Varieties Seeds. J. Seed Res 4(1): 45-59. (In Persian, with English Abstract)
De Figueiredeo, E., M. C. Albuquerque, and N. M. Carvalho. 2003. Effect of the type of environmental stress on the emergence of sunflowers (Helianthus annua L.), soybean (Glycine max L.) and maize (Zeamays L.) seed with different levels of vigor. Seed Sci. Technol. 31: 531-540.
Ebadi, A., Q. Permon, and S. Jahanbakhsh. 2016. Effect seed priming ion seedling vigour index in seed aged milk thistle (Silybum marianum) seeds. J. Plant Res 29(3): 553-566. (In Persian, with English Abstract)
Ehsanullah, K., and M. I. Smith. 2002. Soaking injury in relation to the rate of imbibitions in soybean (Glycine max L. Merrill). Pakistan J. Seed Sci. Technol. 1(1): 27- 33.
Enayati, V., A. Esfandiari, S. M. H. Al Hashem, and A. Hozouri. 2013. The effect of seed exhaustion on germination and heterotrophic growth indicators of corn seedlings. J. Seed Res. 4(12): 61-67. (In Persian)
Escui, B., A. Majidi Harvan, A. Hamidi, F. Moradi, and A. Moghadam. 2014. Investigating the effect of accelerated aging time on seed germ of different sizes and shapes of hybrid single cross 705 corn seeds. Seed Sci. Res. 2(1): 45-53. (In Persian)
Esterbauer, H., and D. Grill. 1978. Seasonal variation of glutathione and glutathione reductase in needles of Picea abies. Plant Physiol. 61:119–121.
Fan, H. F., C. X. Du, L. Ding, and Y. L. Xu. 2013. Effects of nitric oxide on the germination of cucumber seeds and antioxidant enzymes under salinity stress. Acta Physiol. Plant. 35: 2707-2719.
Farooq, M. A., A. K. Niazi, J. Akhtar, M. Saifullah, Z. Farooq, N. Souri, and Z. Rengel. 2019. Acquiring control: the evolution of ROS-induced oxidative stress and redox signaling pathways in plant stress responses. Plant Physiol. Biochem.141: 353–369.
Farooq, M., S. M. A. Basra, R. Tabassum, and I. Afzal. 2006. Enhancing the performance of direct seeded fine rice by seed priming. Plant Prod. Sci. 9: 446-756.
Faruk, O., and L. Matuana. 2008. Nanoclay reinforced HDPE as a matrix for wood-plastic composites. Compos. Sci. Technol. 68: 2073-2077.
Gholami, Sh., and M. A. Dehaghi. 2022. The effect of priming with different concentrations of selenium on germination indices of quinoa seedlings (Chenopodium quinoa Willd.). Agronomy. 24(1): 85-95. (In Persian, with English Abstract)
Giannopolitis, C. N., and S. K. Ries, 1977. Suoeroxide dismutase. I. Occurrence in higher plants. J. Plant Physiol. 59: 309-314.
Govindaraj, M., P. Masilamani, V. Alex Albert, and M. Bhaskaran. 2017. Role of antioxidants in seed quality. A review Agri. Rev. 91(9): 810-830.
Hosseini, A., and A. Koocheki. 2007. The effect of different priming treatments on germination percent and mean germination time of four varieties of sugar beet. J. Agron. Res.5: 69-76.
Hussein, H. J. 2017. The Effect of Seed Priming with Salicylic Acid and Ascorbic Acid on Viability of Local Okra (Abelmoschus Esculentus L.) Seeds Stored for Three Years. J. Global Pharma Technol. 8(9): 110-115.
ISTA. 2012. International Rules for Seed Testing. The International Seed Testing Association (ISTA). Bassersdorf, Switzerland.
Kapilan, R. 2015. Accelerated aging declines the germination characteristics of the maize seeds. Scholars Acad. J. Biosci. 3(8): 708-711.
Kapoor, N., A. A. Mohd, A. Siddiqui, H. Kumar, and A. Amir. 2011. Physiological and biochemical changes during Seed deterioration in gged seeds of Rice (Oryza sativa L.). American J. Plant Physiol. 6: 28-35.
Kaur, S., A. K. Gupta, and N. Kaur. 2003. Priming of chickpea seeds with water and mannitol overcomes the effect of salt stress on seedling growth. Int. Chickpea Pigeon Pea Newslet. 10: 18-20.
Khatami, S. R., M. Sedghi, and R. Seyed Sharifi. 2018. Investigating the effect of priming on the activity of antioxidant enzymes of hybrid single cross 704 corn seeds under drought stress. Iranian J. Seed Sci. Technol 6(2): 198-185. (In Persian)
Khoraki, M., and R. Farhoudi. 2021. Effect of halo priming on germination and seedling growth of single cross 704 corn seeds under salinity stress condition. Seed Sci. Res 7(4): 447-461. (In Persian, with English Abstract)
Khushvagti, H. 2019. Investigating the effect of seed priming on the morphological traits of corn seeds. The 4th International Congress of Agricultural Development, Natural Resources. 13-15 Feb, Tabriz, Iran. (In Persian)
Kibinza, A., J. Bazin, C. Bailly, J. M. Farrant, and F. Corbineau., 2011. Catalase is a key enzyme in seed recovery from ageing during priming. Plant Sci. 181: 309-315.
Kim, D. H. 2018. Extending populous seed longevity by controlling seed mosisture content and temperature. PLoS ONE. 13(8): e0203080.
Kumuri, N., P. Kumar, B. Rai Bura, and J. singh. 2017. Effect of halo priming and hormonal priming on seed germination and seedling vigor in maize (Zea mays L.) seeds. J. Pharm. Pyhtochem. 6(4): 27-30.
MacAdam, J. W., R. Nelson, and E. sharp. 1992. Peroxidase activity in the leaf elongation zone of tall fescue. Plant Physiol. 99: 872-878.
Mojereb, S., M. Moghadam, A. Saeedi Pooya, and R. Narimani. 2018. Effect of hydro and osmopriming on germination improvement of native turf grass of Lolium rigidum. Iranian J. Seed Sci. Technol 6(2): 67-76. (In Persian, with English Abstract)
Murray, S., and H. R. Eisvand. 2018. The effect of priming with salicylic acid and ascorbic acid on germination indices and biochemical traits in wheat seed deterioration. Seed Sci. Res 6(3): 398-381. (In Persian, with English Abstract)
Namdari, A., and F. Sharifzade. 2018. The restoring influence of priming treatments on germination of Smooth vetch (Vicia dasycarpa) under drought stress and maintaining this advantage following aging by using post priming heat shock treatment. J. Plant Biol 10(3): 41-54. (In Persian, with English Abstract)
Nascimento, W. M., and S. H. West. 1999. Muskmelon transplant production in response to seed priming. Hortic. Technol. 9: 53-55.
Paparella, S., S. Araujo, G. Rossi, M. WijaYasingh, D. Carbonera, and A. Balestrazzi. 2015. Seed priming: State of the art and new perspectives. Plant Cell Rep. 89(1): 1-11.
Rezaei, M., M. Sedghi, and R. Seyed Sharifi. 2012. The effect of salinity and types of priming on the growth characteristics of the medicinal plant Calendula officinalis L. Second National Congress of Organic and Conventional Agriculture. 29-31 August, Ardabil, Iran. (In Persian)
Saadat, H., and M. Sedghi. 2021. Effect of priming and aging on Physiological, biochemical traits seed common bean (Phaseolus vulgaris L.). J. Seed Res 11(3): 75-87. (In Persian, with English Abstract)
Saadat, T., H. Alidost, and M. Sedghi. 2019. The effect of priming and exhaustion on the germination of rice seed masses with different strength. J. Seed Res. 10(4): 60-67. (In Persian)
Saadat, T., M. Sedghi, A. Gholipouri, R. Seyed Sharifi, and R. Sheykhbaglou. 2020a. The effect of priming deterioration on the activity of antioxidant enzymes and the mobility of seed reserves in French bean (Phaseolus vulgaris L.) cv. Sadri. Iranian J. Seed Sci. Technol 8(2): 19-32. (In Persian, with English Abstract)
Saadat, T., M. Sedghi, A. Gholipouri, R. Seyed Sharifi, and R. Sheykhbaglou. 2020b. Effect of seed priming and aging on germination, biochemical traits and antioxidant enzyme gene expression in common bean (Phaseolus vulgaris l.). Seed Sci. Res 7(1): 1-13. (In Persian, with English Abstract)
Saddiq, M. S., S. Iqbal, I. Afzal, A. M. H. Ibrahim, M. A. Bakhtavar, M. B. Hafeez, J. Maqbool, and M. M. Maqbool. 2019. Mitigation of salinity stress in wheat (Triticum aestivum L.) seedlings through physiological seed enhancements. J. Plant Nutr. 42(10): 1 -13.
Salehzadeh, H., M. Izadkhah, M. Giyasi, F. Ferozin, and A. Abbasi. 2008. The effect of seed priming on the germination and growth of wheat seedlings (Triticum aestivum L.). Res. J. Biol. Sci 4: 631-629. (In Persian, with English Abstract)
Scot, S. J., R. A. Jones, and W. A. Willams. 1984. Review of data analysis methods for seed germination. Crop Sci 24:1192-1199.
Segund, M., M. Nasr Isfahani, and F. Hadi. 2019. The effect of osmopriming with KNO3 and KH2PO4 germination indicators on seed improvement and growth of Sarhargol seedlings. MS. Thesis. Univ. Lorestan. Iran.
Singh Gill, S., and N. Tuteja. 2010. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol. Biochem. 48: 909-930.
Singh, V., R. S. Upadhyay, B. K. Sarma, and H. B. Singh. 2016. Seed bio-priming with Trichoderma asperellum effectively modulate plant growth promotion in pea. Int. J. Agric. 9(3): 361-365.
Sivritepe, N., H. O. Sivritepe, and A. Eris. 2003. The effects of NaCl priming on salt tolerance in melon seedlings grown under saline conditions. Sci. Hortic. 97(3-4): 229-237.
Siyadat, S. A., M. Sharafizadeh, and S. A. Mousavi. 2011. The effect of priming hormone on the reduction of corn seed burnout. (In Persian, with English Abstract) Quart. J. Plant Physiol. 3(10): 83-67.
Soltani, A., M. J. Robertson, B. Torabi, M. Yousefi-Daz, and R. Sarparast. 2006. Modeling seedling emergence in chickpea as influenced by temperature and sowing depth. Agric. For. Meteorol.138: 156-167.
Suhani, M. M. 1389. Seed control and certification. Gilan University Publications.
Tabatabai, S. F., G. Grineh, G. Fathi, and S. A. Siaydat. 2013. Effect of osmo and hydropriming on Seed Germination, seedling establishment and grain yield of wheat cultivars under Khoozestan climatic conditions. Iranian J. Seed Sci. Technol 2(1): 101-104. (In Persian, with English Abstract)
Tajbakhsh, M., and M. Gheazi. 2011. A summary of the mechanisms of the effect of pre-sowing seed treatments on the improvement of germination and seedling growth in the conditions of biotic and abiotic environmental stress. The second national conference on climate change and its impact on agriculture and environment. 2 August, Oromiyeh, Iran. (In Persian)
Thirajah, M., and R. Kapilan. 2015. Effect of aging on the germination characteristics and enzyme activity of sunflower seeds. IJISR. 2(6): 147-150.
Xu, N., K. Yrle, P. O. Miler, and N. Chaichi. 2004. Co regulation of ear growth and internode elongation in corn. Plant Growth Regul. 44(3): 231-241.
Zheng, M., Y. Tao, S. Hussain, Q. Jiang, S. Peng, J. Huang, and L. Nie. 2016. Seed priming in dry direct-seeded rice: consequences for emergence, seedling growth and associated metabolic events under drought stress. Plant growth Regul. 78(2): 167-178.
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