نوع مقاله : مقاله پژوهشی
نویسندگان
1 استادیار، گروه زراعت، واحد اهواز، دانشگاه آزاد اسلامی، اهواز، ایران.
2 دانش‌آموخته کارشناسی ارشد، گروه زراعت، واحد اهواز، دانشگاه آزاد اسلامی، اهواز، ایران
چکیده
این تحقیق، بصورت فاکتوریل در قالب طرح کاملاً تصادفی با چهار تکرار اجرا شد. تیمارهای مورد مطالعه، شامل تنش شوری بصورت آبیاری با آب معمولی (4/0) و با شوری 4 و 8 دسی‌زیمنس بر متر نمک NaCl و پرایمینگ بذر با پراکسید هیدروژن شامل عدم پیش تیمار و پرایمینگ بذر در محلول 25 و 50 میلی‌مولار بود. در این آزمایش، صفات درصد جوانه زنی، سرعت جوانه زنی، طول ریشه چه، طول ساقه چه، ماده خشک ریشه چه، ماده خشک ساقه چه و شاخص بنیه بذر مورد بررسی قرار گرفت. در این تحقیق اثر تنش شوری و پراکسید هیدروژن برکلیه صفات مورد بررسی معنی‌دار بود. اثر متقابل تنش شوری و پراکسید هیدروژن بر صفات مورد مطالعه بجز سرعت جوانه زنی معنی‌دار شد. بیشترین درصد جوانه زنی، طول و ماده خشک ریشه چه و ساقه چه و شاخص بنیه بذر در شرایط عدم وجود تنش (آب معمولی) و کاربرد 50 میلی‌مولار پراکسید هیدروژن و کمترین آنها در شرایط شوری 8 دسی‌زیمنس بر متر و عدم کاربرد پرکسید هیدروژن حاصل گردید. بیشترین سرعت جوانه زنی در شرایط کاربرد 50 میلی مولار پراکسید هیدروژن و عدم وجود شوری حاصل شد. بطورکلی نتایج نشان داد که کاربرد پراکسید هیدروژن در شرایط وجود تنش شوری از طریق بهبود مؤلفه‌های جوانه زنی مؤثر بود و این می‌تواند به عنوان یک راهکار در جهت استقرار بهتر و مناسب گیاهچه مد نظر قرار گیرد.
کلیدواژه‌ها
Abd El-Monem, A.A., S.F. El-Habbasha, and M. Hozayn. 2013. Mitigation salinity stress effects on barley (hordeum vulgare l.) growth, yield and some physiological aspects by hemin. J. Appl. Sci. Res. 9(3):
2411-2421.
Ahmed, I.M., F. Cao, M. Zhang, X. Chen, G. Zhang, and F. Wu. 2013. Difference in yield and physiological features in response to drought and salinity combined stress during anthesis in Tibetan wild and cultivated barleys. PLOS ONE. 8(10): e77869. Doi:10.1371/journal.pone.0077869.
Akbarimoghaddam, H., M. Galavi, A. Ghanbari, and N. Panjehkeh. 2011. Salinity effects on seed germination and seedling growth of bread wheat cultivars. Trakia J. sci. 9(1): 43-50.
Akter Orthy, M. 2017. Induction of salt tolerance capabily in Wheat (Triticum aestivum L.) through hydrogen peroxide (H2O2) priming. M.S. Thesis. Sher-e-Bangla Agricultural University, Dhaka, Bangladesh.
Ansari, O., and F. Sharif-Zadeh. 2012. Osmo and hydropriming improvement germination characteristics and enzyme activity of Mountain Rye (Secale montanum) seeds under drought stress. J. Stress Physiol. Biochem. 8: 253-261.
Ashraf, M.A., R. Rasheed, I. Hussain, M. Iqbal, M.Z. Haider, S. Parveen, and M.A. Sajid. 2015. Hydrogen peroxide modulates antioxidant system and nutrient relation in maize (Zea mays L.) under water deficit conditions. Arch. Agron. Soil Sci. 61(4): 507-523. Doi:10.1080/03650340.2014.938644.
Bakhshandeh, M. 2010. Groundwater drainage problems in Khuzestan province with a look at an experience. Pp 117-126. In Proc. the 6th drainage and environment technical workshop. Khuzestan, Iran. 06 Jan. 2010. Iranian Natl. Comm. Irrig. Drain., Khuzestan, Iran. (In Persian)
Basra, S.M.A., M. Farooq, and A. Khaliq. 2003. Comparative study of pre-sowing seed enhancement treatment in indica rice (Oryza sativa L.). Pak. J. Life Soc. Sci. 1(1): 5-9.
Cavusoglu, K., and K. Kabar. 2010. Effects of hydrogen peroxide on the germination and early seedling growth of barley under NaCl and high temperature stresses. EurAsian J. Biosci. 4: 70-79. Doi:10.5053/EJOBIOS.2010.4.0.9.
De Azevedo Neto, A.D., J.T. Prisco, J. Eneas-Filho, J.V.R. Medeiros, and E. Gomes-Filho. 2005. Hydrogen peroxide pre-treatment induces salt-stress acclimation in maize plants. J. Plant Physiol. 162(10): 1114-1122. Doi:10.1016/j.jplph.2005.01.007.
Dheeba, B., P. Sampathkumar, and K. Kannan. 2015. Fertilizers and mixed crop cultivation of chromium tolerant and sensitive plants under chromium toxicity. J. Toxicol. 9 (1): 51-60. Doi:10.1155/2015/367217.
Fernandez, I.C.D., E.G. Luque, F.G. Mercado, W. Pedrosa. 2016. Influence of temperature and salinity on the germination of Limonium tabernense Erben from Tabernas desert (Almeria SE Spain). Flora. 218: 68-74. Doi:10.1016/j.flora.2015.12.001.
Hameed, A., and N. Iqbal, 2013. Chemo-priming with mannose, mannitol and H2O2 mitigate drought stress in wheat. Cereal Res. Commun. 42(3): 450-462. Doi:10.1556/CRC.2013.0066.
Hampton, J.G., and D.M. TeKrony. 1995. Hand book of Vigour Test Methods. International Seed Testing Association (ISTA). Zurich, Switzerland.
Ibrahim Ehab, A. 2016. Seed priming to alleviate salinity stress in germinating seeds. J. Plant Physiol. 192: 38-46. Doi:10.1016/j.jplph.2015.12.011.
Ibrahim, M.E.H., X. Zhu, G. Zhou, and E.H. Abidallhaa, 2016. Effects of nitrogen on seedling growth of wheat varieties under salt stress. J. Agric. Sci. 8: 131. Doi:10.5539/jas.v8n10p131.
Jafarian, T., and M.J Zarea. 2017. Evaluating the effect of seed treatment with hydrogen peroxide on anatomical and physiological characteristics of wheat under dry condition. Iranian J. Field Crops Res. 15(3): 627-638. Doi:10.22067/gsc.v15i3.51537.
Jamil, M., C.C. Lee, S.U. Rehman, D.B. Lee, M. Ashraf, and E.S. Rha. 2005. Salinity (NaCl) tolerance of Brassica species at germination and early seedling growth. Electron. J. Envrion. Agric. Food Chem. 4: 273-282.
Jisha, K.C., and J.T. Puthur. 2015. Seed priming with BABA (β-amino butyric acid): a cost-effective method of abiotic stress tolerance in Vigna radiata (L.) Wilczek. Protoplasma. 253(2): 227-289. Doi:10.1007/s00709-015-0804-7
Kanbar, A., and I. El drussi. 2014. Effect of salinity stress on germination and seedling growth of Barley (Hordeum vulgare L.) varieties. Adv. Environ. Biol. 8(1): 244-247.
Kathiresan, A., H.R. Lafitte, J. Chen, L. Mansueto, R. Bruskiewich, and J. Bennett. 2006. Gene expression microarrays and their application in drought stress research. Field Crops Res. 97: 101-110. Doi:10.1016/j.fcr.2005.08.021.
Khan, M., M. Shafi, J. Bakht, and S. Anwar. 2017. Effect of salinity and seed priming on growth characters of wheat varieties. Sarhad J. Agric. 33(3): 435-446. Doi:10.17582/journal.sja/2017/33.3.435.446.
Khayat, M., M. Shirin, M.H. Gharineh, and N.A. Sajedi. 2011. Effectives of different concentrations of sodium hypochlorite and different times of disinfestations on wheat chamran variety. New Findings Agric. 5(4): 367-376. (In Persian, with English Abstract)
Kilic, S. and A. Kahraman. 2016. The mitigation effects of exogenous hydrogen peroxide when alleviating seed germination and seedling growth inhibition on salinity-induced stress in barley. Pol. J. Environ. Stud. 25(3): 1053-1059. Doi:10.15244/pjoes/61852.
Kochaki, A., and G. Sarmadnia. 2007. Crop physiology. Mashhad University. Mashhad, Iran. (In Persian)
Lariguet, P., P. Ranocha, M. De Meyer, O. Barbier, C. Penel, and C. Dunand. 2013. Identification of a hydrogen peroxide signaling pathway in the control of light-dependent germination in Arabidopsis. Planta. 238: 381-395. Doi: 10.1007/s00425-013-1901-5.
Liheng, H., G. Zhiqiang, and L. Runzhi. 2009. Pretreatment of seed with H2O2 enhances drought tolerance of wheat (Triticum aestivum L.) seedlings. Afr. J. Biotechnol. 8(22): 6151-6157.
Li, S.W., L.G. Xue, S.J. Xu, and L.Z. An. 2009. Hydrogen peroxide acts as a signal molecule in the adventitious root formation of mung bean seedlings. Environ. Exp. Bot. 65: 63-71. Doi:10.1016/j.envexpbot.2008.06.004.
Lu, J., X.N. Li, Y.L. Yang, L.Y. Jia, J. You, and W.R. Wang. 2013. Effect of hydrogen peroxide on seedling growth and antioxidants in two wheat cultivars. Biol. Plant. 57(3): 487-494. Doi: 10.1007/s10535-013-0312-6.
Maguire, J.D. 1962. Speed of germination - aid in selection and evaluation for seedling emergence and vigor. Crop Sci. 2: 176-177. Doi:10.2135/cropsci1962.0011183X000200020033x.
Mosavian, S.N., and M. Eshraghi-Nejad. 2013. The effects of seed size and salinity on seed germination characteristic in wheat (var. Chamran). Int. J. Farm. Alli. Sci. 2(2): 1379-1383.
Muller, K., B. Hess, and G. Leubner-Metzger. 2005. A role for reactive oxygen species in endosperm weakening. Pp 287-295. In Seeds: biology, development and ecology. Proc. the 8th Int.. Workshop on Seeds, May 2005. Brisbane, Australia.
Munns, R., and M. Tester. 2008. Mechanisms of salinity tolerance. Annu. Rev. Plant Biol. 59: 651-681. Doi:10.1146/annurev.arplant.59.032607.092911.
Musyimi, D.M., G.W. Netondo, and G. Ouma. 2007. Effects of salinity on growth and photosynthesis of avocado seedling. Int. J. Bot.3: 78-84. Doi: 10.3923/ijb.2007.78.84.
Nazar, R., N. Iqbal, S. Syeed, and N.A. Khan. 2011. Salicylic acid alleviates decreases in photosynthesis under salt stress by enhancing nitrogen and sulfur assimilation and antioxidant metabolism differentially in two mungbean cultivars. J. Plant Physiol. 168: 807-815. Doi:10.1016/j.jplph.2010.11.001.
Nimir, N.E.A., S. Lu, G. Zhou, B.L. Ma, W. Guo, and Y. Wang. 2014. Exogenous hormones alleviated salinity and temperature stresses on germination and early seedling growth of sweet sorghum. Agron. J. 106:2305–2315. Doi:10.2134/agronj13.0594.
Paparella, S., S.S. Araujo, G. Rossi, M. Wijayasinghe, D. Carbonera, and A. Balestrazzi. 2015. Seed priming: state of the art and new perspectives. Plant Cell Rep. 34: 1281-1293. Doi:10.1007/s00299-015-1784-y.
Patade, V.Y., K. Maya, and A. Zakwan. 2011. Seed priming mediated germination improvement and tolerance to subsequent exposure to cold and salt stress in capsicum. Res. J. Seed Sci. 4 (3): 125-136. Doi:10.3923/rjss.2011.125.136.
Rajput, N., A. Kumar, and A.K. Chaudhry, 2015. Effects of NaCl stress on seed germination and early seedling growth of castor bean (Ricinus communis L.). Int. J. Sci. Res. 11: 104-107.
Ramezani, E., M.G. Sepanlou, and H.A.N. Badi. 2011. The effect of salinity on the growth, morphology and physiology of Echium amoenum Fisch and Mey. Afr. J. Biotechnol. 10: 8765-8773. Doi:10.5897/AJB10.2301.
Rouhi, H.R., M.A. Aboutalebian, and F. Sharif-Zadeh. 2011. Effects of hydro and osmopriming on drought stress tolerance during germination in four grass species. Int. J. Agric. Sci. 1: 107-114.
Sairam, R.K., and A. Tyagi. 2004. Physiology and molecular biology of salinity stress tolerance in plants. Curr. Sci. 86: 407-421.
Sarika, G., G.V. Basavaraju, K. Bhanuprakash, V. Chaanakeshava, R. Paramesh, and B.N. Radha. 2013. Investigation on seed viability and vigour of aged seed by priming in French bean. J. Veget. Sci. 40: 169-73.
Savvides, A., S. Ali, M. Tester, and V. Fotopoulos. 2016. Chemical priming of plants against multiple abiotic stresses: mission possible? Trends Plant Sci. 21(4): 329-340. Doi:10.1016/j.tplants.2015.11.003.
Sivritepe, H.O., N. Sivritepe, A. Eris, and E. Turhan. 2005. The effects of NaCl pre-treatment on salt tolerance of melons grown under long-term salinity. Sci. Hortic. 106: 568-581. Doi:10.1016/j.scienta.2005.05.011.
Shiyab, S. 2011. Effects of NaCl application to hydroponic nutrient solution on macro and micro elements and protein content of hot pepper (Capsicum annuum L.). J. Food Agric. Environ. 9: 350-356.
Sung, F.J. 1997. Biochemical activities associated with priming of sweet corn seeds to improve vigor. Seed Sci. Technol. 21: 97-105.
Torech, F.R., and L.M. Thompson. 1993. Soils and soil fertility. Oxford University Press, New York, U.S.
Vashev, B., T. Gaiser, T. Ghawana, A. de Vries, K. Stahr. 2010. Biosafor Project Deliv- erable 9: Cropping Potentials for Saline Areas in India, Pakistan and Bangladesh. University of Hohenheim, Hohenheim, Germany.
Verma, G., S. Mishra, N. Sangwan, S. Sharma. 2015. Reactive oxygen species mediate axis cotyledon signaling to induce reserve mobilization during germination and seedling establishment in Vigna radiate. J. Plant Physiol. 184: 79-88. Doi:10.1016/j.jplph.2015.07.001.
Wojtyla, L., K. Lechowska, S. Kubala, and M. Garnczarska. 2016. Different modes of hydrogen peroxide action during seed germination. Front. Plant Sci. 7: 1-16. Doi:10.3389/fpls.2016.00066.
Xing., H.L., L. Tan , L. An , Z. Zhao, S. Wang, and C. Zhang. 2004. Evidence for the involvement of nitric oxide and reactive oxygen species in osmotic stress tolerance of wheat seedlings: inverse correlation between leaf abscisic acid accumulation and leaf water loss. Plant Growth Regul. 42: 61-68.
Xue, Z.Y., D.Y. Zhi, G.P. Xue, H. Zhang, Y.X. Zhao, and G.M. Xia. 2004. Enhanced salt tolerance of transgenic heat (Triticum aestivum L.) expressing a vacuolar Na+/H+ antiporter gene with improved yields in saline soils in the field and a reduced level of leaf Na+. Plant Sci. 167: 849-859. Doi:10.1016/j.plantsci.2004.05.034.
)