نوع مقاله : مقاله پژوهشی
نویسندگان
1 گروه بیوتکنولوژی، دانشکده کشاورزی و منابع طبیعی، دانشگاه بین‌المللی امام خمینی (ره)، قزوین، ایران
2 دانشیار، گروه بیوتکنولوژی، دانشکده کشاورزی، دانشگاه بین المللی امام خمینی (ره)، قزوین، ایران
چکیده
در این پژوهش خصوصیات موفولوژیکی و بیوشیمایی 10 جدایه Pseudomonas fluorescens، تحمل به شوری و تاثیر جدایه‌ها بر جوانه‌زنی و شاخص‌های رشد گیاهچه‌های کنجد تحت تنش شوری بررسی شد. براساس نتایج تمام جدایه‌ها گرم منفی و دارای تحرک مثبت و خاصیت فلورسنتی بودند. قدرت حل‌کنندگی فسفات در محیط جامد برای جدایه‌های P2، P3 و P9 بیشتر از سایر جدایه‌ها بود. توانایی تولید سیدروفور برخی جدایه‌ها بیشتر بود و جدایه‌های منتخب P1، P2، P3، P8 و P10 متحمل به شوری بودند. تاثیر جدایه‌ها بر جوانه‌زنی کنجد در آزمایشی بصورت فاکتوریل شامل دو رقم کنجد، چهار سطح شوری و سه جدایه در قالب طرح کاملاً تصادفی در سه تکرار بررسی شد. براساس نتایج تأثیر باکتری، شوری، رقم و برهمکنش آنها بر درصد و سرعت جوانه‌زنی، شاخص‌های جوانه‌زنی، ضریب آلومتریک و شاخص بنیه طولی و وزنی گیاهچه معنی‌دار بود. با اعمال تنش شوری، جوانه‌زنی و رشد گیاهچه‌ها به طور معنی‌داری کاهش یافت ولی پیش‌تیمار دانه‌های کنجد با جدایه‌های متحمل به شوری موجب افزایش صفات مرتبط با جوانه-زنی و شاخص‌های رشدی ارقام کنجد شد. تاثیر تلقیح با جدایه P9 بر همه صفات ازجمله میزان جوانه‌زنی بیشتر از سایر جدایه‌ها بود، اگرچه این جدایه در ارزیابی اولیه اعمال تنش شوری به باکتری‌ها مقاومت کمتری نسبت به تنش شوری از خود نشان داده بود. از اینرو جدایه P9 از پتانسیل بالایی برای افزایش تحمل به شوری کنجد تحت تنش شوری برخوردار است.
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