Elahe Nikouee; Maryam Mollashahi; Alireza Moshki; Homan Ravanbaksh
Abstract
Due to the fact that Elaeagnus angustifolia seed has a hard shell, the present study was conducted to investigate the types of appropriate treatments for germination of Elaeagnus angustifolia seed. The experiment was done in Semnan. Seed scarification performed in a completely randomized design with ...
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Due to the fact that Elaeagnus angustifolia seed has a hard shell, the present study was conducted to investigate the types of appropriate treatments for germination of Elaeagnus angustifolia seed. The experiment was done in Semnan. Seed scarification performed in a completely randomized design with three replications (12 seed per replication). Experimental treatments to break dormancy of Elaeagnus angustifolia seed included control, 60% sodium hydroxide for 20 and 30 minutes, 98% sulfuric acid for 20 and 30 minutes, 1% oxygenated water for 10 and 20 minutes, physical scraping, 90°C boiling water for 15 minutes and ice water(5 days at a temperature of zero degrees and then 24 hours at a temperature of 25 degrees for 3 days). The results showed that there was a significant difference at the level of 1 % between the germination treatments of Elaeagnus angustifolia seed including of germination percent, germination rate, and mean time of germination seed vitality index, and seed vegetative traits such as root length, stem length, number of leaflets and seed germination indices. The highest germination percentage was 88.67% in 98% sulfuric acid treatment for 20 minutes. Also, the highest seed vigor index (96%), mean root length(48 mm), mean stem length(60 mm) and average number of leaflets (5.7 number) were observed in this treatment. Totally, the results showed that in all parameters examined that Sulfuric acid treatment of 98% for 20 years obtained the highest values, but two treatments of ice water and boiling water prevented the germination of Oleaster seeds.
Leyla Cheheltanan; Ali Tehranifar; Mahmoud Shoor; Seyyed Hossein Neamati; Saeed Khosravi
Abstract
Self-pollination induces a phenomenon known as inbreeding depression in heterozygous plants, which in turn leads to a reduction in seed germination and seedling growth. On the other hand, seed priming with plant hormones such as gibberellic acid is a technical approach that potentially results in the ...
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Self-pollination induces a phenomenon known as inbreeding depression in heterozygous plants, which in turn leads to a reduction in seed germination and seedling growth. On the other hand, seed priming with plant hormones such as gibberellic acid is a technical approach that potentially results in the improves of rapid and continuous seed germination and subsequent plant growth. Therefore, this research aimed to investigate the effect of different concentrations of gibberellic acid on the improvement of seed germination resulting from self-pollination in various varieties of Petunia hybrida in a factorial experiment with three replications as a completely randomized design. The treatments consisted of four levels of gibberellic acid (0, 50, 100, and 150 mg/L) and seeds obtained from self-pollination of Iranian Petunia, Tango Blue, Tritunia Pink Morn, and Tritunia White. The results indicated that seed priming with gibberellic acid, especially at a concentration of 100 mg/L, led to a significant increase in the activity of α-amylase and β-amylase by 6.56% and 1.57%, respectively. This increase resulted in 8.54% rise in soluble sugars content, providing energy that significantly enhanced germination percentage, germination speed, average time required for germination, seed vigor index, germination energy, and the fresh and dry weight of plumule and radicle. Moreover, among different varieties, seeds obtained from self-pollination of Iranian petunia demonstrated a higher germination percentage and germination speed compared to seeds from other varieties, indicating a superior capability in maintaining vigor potential, germination percentage, and germination speed.
Mahboobe Mohammadi; Mohammad Hassan Rashed Mohassel; Ehsan Neamatollahi
Abstract
This experiment was designed to investigate the effect of seed coating with micronutrient elements, regulators and growth stimulants on the characteristics of germination and seedling establishment of sugar beet seeds, and in the form of a completely randomized design with four repetitions in the year ...
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This experiment was designed to investigate the effect of seed coating with micronutrient elements, regulators and growth stimulants on the characteristics of germination and seedling establishment of sugar beet seeds, and in the form of a completely randomized design with four repetitions in the year 2020, in the Razavi Seed and Plant Institute was carried out under laboratory and greenhouse conditions. The treatments of this experiment were different combinations of micronutrient elements, regulators and growth stimulants which included 29 treatments along with a control treatment (without coating). The results showed that germination percentage, daily germination rate, germination rate coefficient, root and shoot length, root and shoot dry weight, and seedling root length index were significantly affected. Seed coating treatments were applied. In general, treatment 21, including macro elements + micro elements + humic acid + Gibberellic acid, with a germination percentage of 97%, seedling length index 13.87, allometric coefficient 0.2232, as the best treatment, increasing the mean You have a witness about the treatment. Also, among the different seed coating treatments, treatment 22, including microelements + humic acid + Gibberellic acid + kaolin, had the highest percentage of seedling establishment with 95%. Seed coating with micronutrients, humic acid and Gibberellic acid had the greatest effect on improving seedling growth and quality and germination percentage.
Parimah Shokouhi Nasab; Hamidreza Balouchi; َAli Moradi; Mohsen Movahhedi Dehnavi
Abstract
This experiment was conducted in order to investigate the effect of seed coating with biochar and activated carbon on some quinoa seed germination indicators, and it was implemented as a factorial in the form of a completely randomized design with four replications in 2019, in Yasouj University's Faculty ...
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This experiment was conducted in order to investigate the effect of seed coating with biochar and activated carbon on some quinoa seed germination indicators, and it was implemented as a factorial in the form of a completely randomized design with four replications in 2019, in Yasouj University's Faculty of Agriculture. The first factor is seed coating in four levels (no coating, coating with activated carbon, coating with biochar and coating with activated carbon + biochar) and the second factor includes salinity stress in four levels (zero, 75, 150 and 225 mM sodium chloride). The results of the interaction of salinity stress and seed coating showed that the highest content of soluble sugar (28.578 mg/g seed FW) and malondialdehyde (2.97 µmol/g seed FW) was obtained by coating with activated carbon in 150 mM salinity stress. Also, the highest amount of seed hydrogen peroxide (0.18 μmol/g seed FW) and proline (10.49 μmol/g seed FW) at the level of 225 mM sodium chloride was obtained by covering the seeds with biochar and active carbon, respectively. Covering seeds with activated carbon and biochar led to an increase in the length of root and shoot under salt stress conditions. However, under salinity stress conditions, root weight and length vigor index of seed improved more by coating seeds with activated carbon. It can be stated that seed coating can greatly reduce the harmful effects of osmotic stress on germination and biochemical traits in quinoa seedlings and improve seedling growth.
Abolghasem Hamidi Moghaddam
Abstract
Henna (Lawsonia inermis L.) is an ornamental and medicinal plant containing Lawson’s active substance, which is cultivated in some regions of the south and southeast of Iran. Salinity is one of the major abiotic environmental stress that affects almost all stages of plant development. Polyamines ...
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Henna (Lawsonia inermis L.) is an ornamental and medicinal plant containing Lawson’s active substance, which is cultivated in some regions of the south and southeast of Iran. Salinity is one of the major abiotic environmental stress that affects almost all stages of plant development. Polyamines such as putrescine can play important roles in plant growth and stress responses. In order to investigate the effect of putrescine and NaCl salinity stress on some germination indices, a factorial experiment was conducted with two levels of putrescine (0 and 0.75 mM) and four salinity levels (0, 50, 100 and 150 mM) based on completely randomized design with four replications in germinator for 11 days. The results showed that at control salinity level, there was no significant difference between putrescine treatments on Henna seed germination indices except the seed vigor index. The interaction between putrescine and salinity stress showed that pre-treatment with putrescine alleviated the negative effects of 50 and 100 mM salinity stress on the germination rate. However, it increased the negative effects of 150 mM salinity stress on germination percentage, radicle length, plumule length, seedling fresh weight, seed vigor index and α-amylase activity in comparison to control. The results showed that germination of Henna seeds is sensitive to salinity stress and they are only able to tolerate low levels of salinity (50 mM). According to a significant correlation between α-amylase activity with germination percentage (r= 0/98), it can be concluded that probably the change in α-amylase activity decreased the germination percentage.
Nesa Gharehbaghli
Abstract
With the aim of evaluating the effect of salicylic acid in improving the efficiency of the aged pumpkin seeds under drought stress conditions, a factorial experiment was carried out in the form of a completely randomized design with three replications. Pretreatment of different concentrations of salicylic ...
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With the aim of evaluating the effect of salicylic acid in improving the efficiency of the aged pumpkin seeds under drought stress conditions, a factorial experiment was carried out in the form of a completely randomized design with three replications. Pretreatment of different concentrations of salicylic acid including zero (pretreated with water), 1 and 1.5 mM at different drought levels of zero, -0.2, -0.4 and -0.6 MPa were investigated. Indicators of mean germination time, germination percentage, seedling length, seed vigor, membrane electrolyte leakage, soluble carbohydrates, soluble proteins, malondialdehyde, ascorbate peroxidase activity, superoxide dismutase and catalase enzymes were evaluated. The results showed that the pretreatment of seeds with different concentrations of salicylic acid prevented the significant decrease in the germination indices of the deteriorated pumpkin seeds under drought stress. So that at the potential of -0.6 MPa, the treatment of seeds with 1.5 mM salicylic acid increased the percentage of germination, seed germination index, soluble carbohydrates and soluble proteins respectively 80.2, 169.4, 51.6, 93.4% and for the activity of ascorbate peroxidase, superoxide dismutase and catalase enzymes, 41.2%, 12% and 11.8%, respectively, compared to the control. Based on the research findings, pretreatment of salicylic acid (1.5 mM) is recommended in order to reduce the oxidative stress caused by drought for pumpkin.
Seyed Keyvan Marashi; Zahra Albaji
Abstract
This research was conducted as a factorial in a completely randomized design with four replications. The studied treatments include salinity stress in the form of irrigation with normal water (0.4) and with salinity of 4 and 8 dS/m of NaCl salt and seed priming with hydrogen peroxide, including no priming ...
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This research was conducted as a factorial in a completely randomized design with four replications. The studied treatments include salinity stress in the form of irrigation with normal water (0.4) and with salinity of 4 and 8 dS/m of NaCl salt and seed priming with hydrogen peroxide, including no priming and seed priming in 25 and 50 mM solutions. In this experiment, the traits of germination percentage, germination rate, root length, stem length, root dry matter, stem dry matter and seed vigor index were investigated. In this research, the effect of salinity stress and hydrogen peroxide was significant on all investigated traits. The interaction effect of salinity stress and hydrogen peroxide on the studied traits was significant except germination rate. The maximum percentage of germination, length and dry matter of root and stem and seed vigor index was obtained under absence of stress (normal water) and application of 50 mM hydrogen peroxide and the minimum was under salinity of 8 dS/m and no application hydrogen peroxide. The maximum germination rate was obtained in the 50 mM hydrogen peroxide and no salt stress. In general, the results showed that the use of hydrogen peroxide in the presence of salinity stress was effective through the improvement of indices, and this can be considered as a strategy for better and proper seedling establishment.
Ali najm; Mehdi Aran; Abdolrahman Rahimian Boogar Rahimian Boogar
Abstract
This experiment was conducted to investigate the germination and seedling growth of two papaya (Carica papaya L.) varieties in response to the inoculation of mycorrhizal fungi and culture substrates in a factorial in the form of a completely randomized design. The experiment factors include varieties ...
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This experiment was conducted to investigate the germination and seedling growth of two papaya (Carica papaya L.) varieties in response to the inoculation of mycorrhizal fungi and culture substrates in a factorial in the form of a completely randomized design. The experiment factors include varieties at 2 levels (Red Lady (V1) and Bangladeshi (V2)), culture substrate at 5 levels (compost + perlite + vermicompost (1:1:1) (S1), compost + perlite + cocopeat (1:1:1) (S2), compost + vermicompost + cocopeat (1:1:1) (S3), perlite + vermicompost + cocopeat (1:1:1) (S4) and compost + perlite + vermicompost + cocopeat (1:1:1:1) (S5)), and mycorrhizal at 2 levels (no inoculation as control (M0) and inoculation (M1)). The results indicated a significant effect of culture substrate and mycorrhizal on germination and growth characteristics of papaya varieties. In the mycorrhizal inoculation treatment, the highest germination percentage (77.33%) and germination speed index (0.63) were observed in the Bangladeshi variety and the lowest average germination time for both varieties . The highest seedling height and root length were recorded for V1S4M1 and V2S5M1 treatments, and the highest shoot dry weight (0.276 g) was recorded for V2S5M1 treatment. According to the obtained results, inoculation of seeds in both Red Lady and Bangladeshi papaya varieties with mycorrhizal fungi and substrates containing organic matter, especially vermicompost, improved the germination and growth characteristics of the seedlings. Therefore, according to the advantages and compatibility of mycorrhizal fungi, it can be used as a suitable treatment to improve the germination and growth of papaya seedlings.
Mahboobe Bahrami; Mohamad Rahim Owji; farhad mohajeri; Mahmood Dejam
Abstract
Salinity stress reduces seed quality, but suitable concentrations of silicon and nickel can improve germination indices in the produced seeds. This study was conducted in a factorial arrangement based on randomized complete block design (RCBD) in 2018 in Haji Abad city, Hormozgan, Iran. The experimental ...
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Salinity stress reduces seed quality, but suitable concentrations of silicon and nickel can improve germination indices in the produced seeds. This study was conducted in a factorial arrangement based on randomized complete block design (RCBD) in 2018 in Haji Abad city, Hormozgan, Iran. The experimental treatments consisted of four safflower varieties as the first factor, including Soffeh, Local Isfahan, Padideh, and Golmehr; and seven spraying levels, including nickel (100, 200 and 300 mg L-1) and silicon (100, 150, and 200 mg L-1) as the second factor. The results demonstrated that the foliar application of 100 mg/l Nickle and 200 mg/l silicon increased potassium by 10% and 18%, respectively. The foliar application of 200 mg/l silicon increased the activity of alpha and beta amylase enzymes by 17% and 12%, respectively. In addition, Golmehr and Safe varieties obtained from a mother plant recorded higher germination and rate percentage, as well as seed vigor, while Padideh variety showed the lowest values of the same traits. The foliar application of 200 mg/l silicon, compared to the control, increased the seed vigor as well as Soffeh and Golmehr cultivars by 23% and 25%, respectively; however, the Nickle concentration of 300 mg/l resulted in a decrease of 24% and 19% in these cultivars.On the other, this trait decreased with increasing nickel concentration significantly. Generally, foliar application of silicon at concentrations of 150 and 200 mg L-1 was found to improve seed quality of different safflower varieties under salinity stress.
mahdi Afrouz; Parisa Sheikhzadeh
Abstract
TIn order to evaluate the effect of iron nanooxide synthesized from oregano on germination, growth and biochemical characteristics of corn seedlings, an experiment was carried out in a randomized complete block design with three replications. Experimental factors included synthesized iron oxide nanoparticles ...
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TIn order to evaluate the effect of iron nanooxide synthesized from oregano on germination, growth and biochemical characteristics of corn seedlings, an experiment was carried out in a randomized complete block design with three replications. Experimental factors included synthesized iron oxide nanoparticles and the application methods of iron oxide nanoparticles. The results showed that iron oxide nanoparticles had a significant effect on germination, growth, and biochemical characteristics of seedlings. The highest germination rate, vigor index, seedling dry weight, and length were achieved with the application of 240 mg L-1 of iron oxide nanoparticles. Among the nanoparticle application methods, seed priming compared to the method of adding them to the planting medium had a greater effect on improving germination, growth, and increasing the vigor index. Using 240 mg L-1 of iron oxide nanoparticles as priming resulted in the highest germination percentage, germination synchrony index, mean daily germination, and the lowest MGT. In the case of adding nanoparticles to the planting medium, although it had less effect than the priming method, adding 360 mg L-1 of iron oxide nanoparticles to the planting medium also caused a significant increase in corn germination. The use of different concentrations of iron oxide nanoparticles in both methods of nanoparticle application caused a significant increase in the activity of antioxidant enzymes in seedlings. Therefore, the use of a concentration of 240 mg L-1 of iron oxide nanoparticles as a priming can be suggested to improve germination, growth, and biochemical characteristics of seedlings.
Masoomeh Amerian; Mahmoud Khoramivafa; Barakat Alah Rabani
Abstract
in this study, the effect of silver nanoparticles and humic acid on some morphological characteristics of quinoa seedlings under drought stress was investigated. The experiment was performed as a factorial experiment in a completely randomized design with three replications. The experiment consisted ...
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in this study, the effect of silver nanoparticles and humic acid on some morphological characteristics of quinoa seedlings under drought stress was investigated. The experiment was performed as a factorial experiment in a completely randomized design with three replications. The experiment consisted of three levels of silver nanoparticles (0, 10 and 20 mg L-1 AgNPs), three levels of humic acid (0, 100 and 300 mg L-1) and three levels of drought stress (0, -6 and -12 bar). In this study, Titicaca cultivar was used. The highest amount of seed vigor and seed germination rate were observed in the highest level of AgNPs and humic acid, which indicates the positive effect of AgNPs and humic acid on germination percentage, germination rate and quinoa seed vigor. According to the obtained results, drought stress led to a decrease in germination percentage and growth characteristics of quinoa seeds. AgNPs and humic acid had a positive effect on germination and growth characteristics of quinoa seedlings under drought stress conditions compared to control treatment. With increasing the concentration of AgNPs and humic acid, the amount of proline, soluble sugars, total phenol, flavonoids and catalase activity under drought stress increased, indicating the positive role of AgNPs and humic acid to increase drought tolerance in quinoa. Therefore, the use of humic acid at a concentration of 300 mg L-1 along with 20 mg L-1 AgNPs is recommended to improve germination and seed growth of quinoa under drought stress conditions
Fatemeh Primoradi; Abdolmahdi Bakhshandeh; Seyed Amir Moosavi; Mohammad Hossain Gharineh; Ali Moshatati
Abstract
In order to investigate the combined effects of temperature and salinity stress on seed germination properties of ziziphus (Ramlik), a factorial experiment conducted at seed technology laboratory of Agricultural Sciences and Natural Resources University of Khuzestan in 2019. The experiment was arranged ...
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In order to investigate the combined effects of temperature and salinity stress on seed germination properties of ziziphus (Ramlik), a factorial experiment conducted at seed technology laboratory of Agricultural Sciences and Natural Resources University of Khuzestan in 2019. The experiment was arranged as complete block design with three raplications. First factor was salinity (0, 50, 100, 150, 200, 250 and 300 mM) and second factor was temperature (5, 10, 15, 20, 25, 30, 35 and 40 oC). Results of the experiment revealed that there was no seed germination in 5 oC, 10 oC at all salinity treatments. Seed germination and seedling growth were declined at all temperature treatments bythe increase in salinity concentrations. Salinity tolerance threshold of Ramlik at 25 OC was 173 mM but as temperature increased to 35 oC, it declined to 150 mM. The highest seed germination properties of Ramlik were observed at 25 oC. An increase in salinity led to a lower seed germination rate. As Ramlik seeds were capable to complete their germination at
Masoomeh Amerian; Ali Reza Khosravi
Abstract
In order investigate the effect of salinity and selenium on seed germination of three medicinal plants dragons head, alyssum and chicory, this study was performed as a factorial experiment based on a complete randomized design with two factors including different levels of salinity and selenium in three ...
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In order investigate the effect of salinity and selenium on seed germination of three medicinal plants dragons head, alyssum and chicory, this study was performed as a factorial experiment based on a complete randomized design with two factors including different levels of salinity and selenium in three replications. The first factor included 0, 2, 4, and 8 mM NaCl and the second factor was 0, 5, 10, and 20 mg L-1 sodium selenate levels. Unlike selenium, salinity reduced germination percentage, germination characteristics and initial growth in all three medicinal plants. The germination percentage of chicory was 40%, alyssum was 49.1% and dragons head was 52.5%, which indicates that chicory is more sensitive to salinity stress, while dragons head showed more tolerance to salinity stress. With increasing selenium concentration, seedling length in all three medicinal plants increased compared to the control. The highest seedling length (20 mm) was observed in treatment 20 mg L-1 sodium selenate with a concentration of 8 mM NaCl in alyssum. The lowest seedling length was in chicory (6.2 mm) and in 0 mg L-1 sodium selenate treatment with 8 mM NaCl. The use of selenium can improve germination characteristics and to some extent reduce the effects of salinity stress. In general, it can be concluded that selenium (at the level of 20 mg L-1 of sodium selenate) can increase seed germination and seedling growth of all three medicinal plants under salinity stress.
mohsen sasani; Masoud Ahmadzadeh; M.R. Jahansuz; sorayya navid
Abstract
Root and crown rot of wheat is one of the most important diseases that caused by Fusarium species. The present study was conducted with the aim of seed Bioprime with bacteria to control fungal pathogens of root and crown rot and improving some growth indicators of wheat in laboratory and greenhouse conditions ...
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Root and crown rot of wheat is one of the most important diseases that caused by Fusarium species. The present study was conducted with the aim of seed Bioprime with bacteria to control fungal pathogens of root and crown rot and improving some growth indicators of wheat in laboratory and greenhouse conditions in 2019. The Effect of bacterial inhibition on fungal pathogen growth in vitro and in cross-culture test, metabolites and bacterial extracts were 60, 50 and 30 percent respectively. Bacterial isolates were able to produce biocontrol enzymes, metabolites and hormones of growth promoting such as auxin, siderophore, and ACC deaminase. In vitro, infection of wheat seeds before priming had a beneficial effect on increasing the efficiency of bioprime technique with bacteria, because in uninfected wheat seeds, bacteria did not play a role in reducing pathogenic fungi. Bioesmoprim, osmoprime and halloprime had the most impact in controlling the pathogenic fungus and improving the growth indices of wheat (germination percent, length and weight of root and stem) in greenhouse conditions, respectively. Although Talc powder and gum in germinated seeds reduced the fungal disease of root and crown rot, but seed germination percentage of wheat seeds were reduced, too. The combination of talc powder + xanthan gum with bacteria improved the mentioned traits.
seyyed esmaeil mousavi; Heshmat Omidi
Abstract
To study the effect of biological treatment on germination and growth indices and pigments of flaxseed under salinity stress, a factorial experiment in a completely randomized design was conducted in three replications at the faculty of agriculture of Shahed University in 2018. Experimental treatments ...
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To study the effect of biological treatment on germination and growth indices and pigments of flaxseed under salinity stress, a factorial experiment in a completely randomized design was conducted in three replications at the faculty of agriculture of Shahed University in 2018. Experimental treatments were salinity at four levels (zero, 2.5, 5 and 7.5 dS/m) and biological treatments at four levels (control, bacteria (Baccillus subtilis), fungi (Macrophomina phaseolina) and combination of bacteria and fungi). Effect of biological treatment was significant on germination percentage and the highest germination percentage (99 %) obtained at fungi treatment. Effect of salinity and biological treatment was significant on mean germination time and germination coefficient. The minimum mean germination time (3.79 days) obtained at combination of bacteria and fungi treatment that shows this treatment was most effective in compared with other biological treatments. With increasing of salinity, mean germination time increased. Salinity reduced radicle, plumule and seedling length. The means related to radicle length at the different levels of salinity at biological treatment of fungi was highest amount in compared with other biological treatments. Interaction effect of salinity and biological treatment was significant on chlorophyll a and total chlorophyll. With increasing of salinity, reduced pigments. Maximum amount of chlorophyll a (16.52 µm.ml1-) was obtained at the control treatment of biological treatment and zero salinity. The result of this research showed that using of microorganisms such as fungi and bacteria in salinity stress, can reduce the negative effects of salinity on germination and growth of this plant.
ahmad zare; Zeinab Porameri
Abstract
In order to knowledge the dormancy and seed germination of field dodder in response to environmental factors (sulfuric acid (physical dormancy), light, temperature, salinity and drought stress) four separate experiments were conducted at agricultural scientists and natural recourses university of Khuzestan. ...
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In order to knowledge the dormancy and seed germination of field dodder in response to environmental factors (sulfuric acid (physical dormancy), light, temperature, salinity and drought stress) four separate experiments were conducted at agricultural scientists and natural recourses university of Khuzestan. Treatments of sulfuric acid (96%) immersion (0, 10, 20, 30, 60, 90 and 120 m exposure to sulfuric acid), temperature (5, 10, 15, 20, 25, 30, 35, 40, 45°C). Light (light/dark and darkness), salinity (0, 50, 100, 150, 200, 250, 300, 350, 400 and 400 mM) and drought stress (0, -0.2,-0.4, -0.6, -0.8, -1 and -1.2 MPa) were considered. Seed immersion for 30 m increased germination up to 100% while by increasing of seed immersion in sulfuric acid to 120 minutes, germination decreased to 60%. Seed germination were at 10°C (53%), 20 (88%), 25 (100%), 30 (100%), 35 (100%) and 40°C (42%). Germination of field dodder wasn’t dependent on light. The salinity required to 50% inhibition for traits germination percentage, germination index and germination rate were predicted 154.89, 139.71 and 119.11mM respectively. From the results of drought stress indicated that for 50% inhibition of germination percentage, germination index and germination rate (logistic equation) were predicted -0.57, -0.43 and 0.52 MPa respectively.
Mohammad khademi; Faezeh Zaefarian; Shahram Nazari; Mohammad Ali Esmaelee
Abstract
In order to investigate the effect of hydropriming and osmopriming on the characteristics of experimental rice seedlings, the treatment was performed in a completely randomized design in three replications.Treatments included two rice cultivars (Tarom and Shiroudi) and priming at five levels including ...
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In order to investigate the effect of hydropriming and osmopriming on the characteristics of experimental rice seedlings, the treatment was performed in a completely randomized design in three replications.Treatments included two rice cultivars (Tarom and Shiroudi) and priming at five levels including calcium chloride and potassium chloride priming (Ψs -1.25 MPa), ascorbate acid (10 mg l-1), hydropriming (for 48 hours) and control (no priming). In this study, seedling characteristics were measured. Interaction of cultivar and priming showed that the highest germination percentage was observed in the range of 97-100% in Shiroudi and Tarom cultivars primed with calcium chloride and potassium chloride. The lowest germination percentage (85.33% and 77.33%, respectively) was obtained under control in Shiroudi and Tarom cultivars, respectively. The highest germination rate was obtained in priming with calcium chloride and potassium chloride, which increased the germination rate by 52 and 54%, respectively. The effect of cultivar and priming on root and shoot length as well as root and shoot dry weight were significant at 1% probability level. also, root and shoot length were 5.97 and 5.4 cm in Shiroudi and 4.01 and 4.59 cm in Tarom, respectively. The results showed that priming with calcium chloride, potassium chloride, ascorbic acid and water increased seedling length vigor indices 69, 64, 13 and 32% in Shiroudi and 91, 70, 47 and 43% in Tarom, respectively compared to control. Also, priming with calcium chloride, potassium chloride, ascorbic acid and water increased seedling weight vigor indices by 110, 95, 50 and 47%, respectively.
Hamid Jabbari; Hossein Zeinalzadeh Tabrizi; Mehran Enayati shariatpanahi
Abstract
This experiment was conducted in a factorial arrangement in a completely randomized design with three replications. 24 canola genotypes (13 varieties and 11 double haploid lines) at four levels of soil moisture including 80, 50, 30 and 20% field capacity (FC) were evaluated in pots in the greenhouse ...
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This experiment was conducted in a factorial arrangement in a completely randomized design with three replications. 24 canola genotypes (13 varieties and 11 double haploid lines) at four levels of soil moisture including 80, 50, 30 and 20% field capacity (FC) were evaluated in pots in the greenhouse of seed and plant improvement Institute in Karaj during 2017. The growth of the shoot was more affected by different levels of soil moisture in comparison to the root growth that indicating a greater sensitivity of the stem length compared to the root length in response to the low water stress. Therefore, the root-shoot ratio can be an appropriate index for assessing the water stress tolerance. The results showed that cultivars such as Oase, Triangle, Tassilo and Savanah, had very high stability under stress conditions, but due to small amounts of the first and second components, they were classified as susceptible to water deficit stress. Also, SLM046 and Billy had the least stability in traits under water deficit stress at germination stage. Due to the non-separation of the three levels of soil moisture (80%, 50% and 30% FC), were not suitable for selection and only 20% FC was identified as the appropriate condition for selecting the ideal genotype. Therefore, between genotypes that were located in a 20% FC, based on the 'which-won-where' pattern, GKH 2005 was identified as the superior genotype.
sholeh bahrami; Mehdi Tajbakhsh; Esmaeil Rezaei-chiyaneh
Abstract
In order to evaluate germination characteristics and shelf life of alfalfa seeds stored in different storage conditions, a factorial experiment was conducted in a completely randomized design in a graduate laboratory of Urmia University of Agriculture. The experiments included relative humidity in six ...
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In order to evaluate germination characteristics and shelf life of alfalfa seeds stored in different storage conditions, a factorial experiment was conducted in a completely randomized design in a graduate laboratory of Urmia University of Agriculture. The experiments included relative humidity in six levels (50, 60, 70, 80 and 90 %) and control (room humidity) and storage duration (0, 1, 2, 3, 4, 5, 6, 7, 8, 9 and 10 months) with three replications. The studied traits included germination indices, seedling growth indices, and seed vigor and storage capability of seeds. The results showed that all germination indices were affected by relative humidity and storage duration. The interaction between moisture and storage duration was significant except for germination uniformity on all indices. The highest germination percentage was obtained in the control conditions (96.9 %), which reached 79.4 after 10 months of storage. Increasing the moisture content of the storage caused a significant decrease in the coefficient and uniformity of germination. The germination rate was the highest in the first month with the average of 0.071 per day, which had the lowest in 90 % relative humidity in the 10 months after storage to the average of 0.018 germs per day.
zahra Mohaddes Ardebili; Hossein Abbaspour; Reza Tavakkol afshari; Seid Mohsen Nabavi Kalat4
Abstract
In order to study the effect of accelerated aging and priming with gibberellic acid on germination characteristics, lipids peroxidation, soluble protein, total phenol and electrical conductivity of wheat seed (Pishtaz Cultivar) an experiment in factorial laid out completely randomized design with three ...
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In order to study the effect of accelerated aging and priming with gibberellic acid on germination characteristics, lipids peroxidation, soluble protein, total phenol and electrical conductivity of wheat seed (Pishtaz Cultivar) an experiment in factorial laid out completely randomized design with three replications was conducted at Seed Technology Laboratory, Department of Agronomy, Faculty of Agriculture, Ferdowsi University, Mashhad, Iran, in 2016. Factors were included of seed aging(100% relative humidity at 40°C) in three levels [low deterioration (4 days), medium deterioration (6 days) and high deterioration (7 days)] and priming with gibberellic acid in four levels (0, 25, 50 and 100 ppm). Analysis of variance showed that the effect of seed aging, priming with gibberellic acid and interaction between two factors on all traits were significant. Based on the means comparison of the traits germination percentage, germination rate, soluble protein and total phenol decreased with increasing aging time. But, the level of malondyaldeid and electrical conductivity increased due to damage to cell membrane with increasing aging time. Investigation of interaction between two factors showed that the priming with gibberellic acid reduced the negative effects of seed aging on all traits.
atfeh hoseini; َAli Moradi; H.R. Balouchi
Abstract
In order to investigate seed bio-priming effects on some germination index of Pimpinella anisum L. under drought stress, this research was done in genetic lab at Agriculture department of Yasouj University. The experiment was factorial with two factor based on CRD design in 4 replications and in each ...
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In order to investigate seed bio-priming effects on some germination index of Pimpinella anisum L. under drought stress, this research was done in genetic lab at Agriculture department of Yasouj University. The experiment was factorial with two factor based on CRD design in 4 replications and in each Petri dishe 30 seeds were planted. First factor was seed bio-priming with nine level (on bio-priming, 4 Bactria strain (P.fluorescent), fluorescent (CHA0), 1) P.F (, 2) P.F (, 16) B (and 52 (B)) and 4 strain of T.harzianum (T29, T32, T43, T36 and T39) and second factor include osmotic potential of drought stress in 3 levels (0, -3 and -6 bar). The evaluated traits in this study included germination percentage, germination rate, seedling length, dry weight, seedling vigour index (weight and length) and alometric coefficient. Results of test showed that increasing drought stress, decreased seed germination indexes, and bio-priming couldn’t improve these indexes than non-priming seeds, therefore in each of drought stress potential, T36 has the maximum germination percentage (83.88), germination rate (4.7), seedling length vigor index (10), seedling dry weight (8.07), and seedling weight vigor index (15.25) and not primed seeds have the lowest amount of this traits. According to the results, it can be stated that the application of bio-priming treatment can moderately reduce the adverse effects of drought stress in the germination stage of pimpinella anisum L.
Samira Parsaie; Mohsen Movahhedi Dehnavi; H.R. Balouchi; Mahmood Attarzadeh
Abstract
Drought stress on maternal plant can negatively affect germination characteristics of produced seeds, and micronutrients application in this circumstance may mitigate drought impacts and leads to seeds with higher vigor. This research was conducted as two experiments in 2015 in seed laboratory of Yasouj ...
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Drought stress on maternal plant can negatively affect germination characteristics of produced seeds, and micronutrients application in this circumstance may mitigate drought impacts and leads to seeds with higher vigor. This research was conducted as two experiments in 2015 in seed laboratory of Yasouj University. At first experiment the seeds obtained from a former experiment included of three irrigation levels (after 75, 110 and 145 mm evaporation from class A pan) and four foliar applications included of water, zinc sulfate 3000 ppm, boric acid 2000 ppm and mixture of zinc sulfate and boric acid, were evaluated in germination experiments in 30°c. Second experiment was a factorial based on RCD with three replications. First factor was drought stress, by PEG 6000, in three levels (0, -0.2 and -0.4 Mpa), and second factor in eight levels included of seed soaking in water, ZnSo4, H3Bo3 and ZnSo4 + H3Bo3 and seed with high content of Zn , B and Zn+ B and a dry seed as control. First experiment results showed that with delaying in irrigation time, foliar application of micronutrients, compared to water foliar application, significantly increased germination traits. In second experiment, although seed soaking in nutrients, significantly decreased germination indices, but seed with high zinc and boron contents positively affected on germination vigor and antioxidant activities especially under drought stress condition.
lila moradi; raouf seyed sharifi
Abstract
In order to study the effects of plant growth promoting rhizobacteria (PGPR) and salinity stress on seed germination parameters, K and Na content of rye (Secale cereal L.) seedling , two factorial experiments based on CRD and RCB design were conducted with three replications at laboratory and greenhouse ...
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In order to study the effects of plant growth promoting rhizobacteria (PGPR) and salinity stress on seed germination parameters, K and Na content of rye (Secale cereal L.) seedling , two factorial experiments based on CRD and RCB design were conducted with three replications at laboratory and greenhouse respectively at faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabil in 2016. Experimental factors were included soil salinity in four levels (no-salt as control, salinity 25, 50 and 75 mM as NaCl) and seed inoculation with plant growth promoting rhizobacteria in four levels (no inoculation as control, inoculation with Pseudomonas, Azosprilium, both application of Pseudomonas and Azosprilium). The results showed that maximum germination components (such as ridicule and plumule length, radicule and plumule dry weight, germination percentage, uniformity of germination) were obtained at co-application of Pseudomonas and Azosprilium under non-saline condition and minimum of these parameters were obtained at 75 Mm NaCl and no inoculation. K/Na ratio in root and shoot were decreased with increasing salinity level. It was vise versa in seed inoculation with plant growth promoting rhizobacteria. Maximum ratio of Na/K was obtained at the highest level salinity and no inoculation seed with PGPR and minimum of it was obtained in both inoculation with Pseudomonas and Azosprilium and the least level of salinity. It seems that application of PGPR can be used as a proper method for increasing K /Na ratio, germination components and seedling growth of rye under salinity stress.
Abstract
Carrot is a particularly difficult crop to manage in terms of weed control. For weed management of carrot, seed germination is a key process because it determines both the number of weeds that could potentially emerge and the timing of their appearance in the carrot. This study was done to evaluate two ...
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Carrot is a particularly difficult crop to manage in terms of weed control. For weed management of carrot, seed germination is a key process because it determines both the number of weeds that could potentially emerge and the timing of their appearance in the carrot. This study was done to evaluate two nonlinear regression models (Intersected-lines and Dent-like) to describe response of germination rate to temperature in carrot (Daucus carota L.), common chickweed (Stellaria media (L.) Vill.), yellow foxtail (Setaria glauca (L.) P. Beauv.) and canada fleabane (Conyza canadensis (L.) Cronq.). This experiment was based on completely randomized design with 4 replications at Islamic Azad University, Science Research Branch, in 2015. The seeds were treated with different temperatures (2, 5, 10, 15, 20, 25, 30, 35, 40 and 45oC). The analysis of variance showed that temperature had a significant effect on all seed germination percentage and germination rates. Intersected-lines model was superior in carrot and Dent-like model was superior for common chickweed, yellow foxtail and canada fleabane. Base, optimum and maximum temperatures were predicted with appropriate model. Base, optimum and maximum temperatures were for carrot 1.67, 22.84, 43.16; common chickweed 3.58, 18.82-19.67, 42.75; yellow foxtail 14.17, 33.75-34.92, 44.86 and canada fleabane 13.74, 31.73-31.94, 44.21ºC, respectively. This results showed that carrot germinated earliest among the studied species, because it had the lowest base temperature, so sooner planting it was caused sooner carrot establishment and less weed competition.
Mahmood Attarzadeh; Hamidreza Balouchi; Mohsen Movahhedi Dehnavi; amin salehi; Majid Rajaie
Abstract
Reduction in germination and growth can be seen in seeds obtained from mother plants which have experienced water stress. From the other point of view utilization of biological fertilizers under environmental stress, the condition can produce seeds with higher germination capacity. This research was ...
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Reduction in germination and growth can be seen in seeds obtained from mother plants which have experienced water stress. From the other point of view utilization of biological fertilizers under environmental stress, the condition can produce seeds with higher germination capacity. This research was conducted as split plot based on a randomized complete block design with three replications in 2016. The main factor included of irrigation regimes at three levels included in soil irrigation after 25, 50 and 75% of soil moisture depletion and a sub-factor of phosphorus supply in six levels included of 100% phosphorus requirement from the source of triple superphosphate, 50% phosphorus + arbuscular mycorrhizal fungus, Mycorrhiza arbuscular fungus, 50% phosphorus + Pseudomonas fluorescens, Pseudomonas fluorescens and a control test without phosphorus fertilizer. Results showed that in 75% of soil moisture depletion, maximum amounts of germination, seed vigor, root length, stem length and seedling dry weight were obtained from 50% of phosphorus + arbuscular mycorrhiza. At irrigation level of 50 and 75% moisture depletion, the application of 50% phosphorus + arbuscular mycorrhiza made an increase in germination rate and uniformity. The electrical conductivity of Echinacea purpurea seeds showed increasing trend with intensification in drought stress. The results of this study showed that in present study Pseudomonas fluorescens had lower improvement effects on percent and rate of germination and the adjustment of drought adverse effects in compared with arbuscular mycorrhiza.
