بررسی تغییرات فیزیولوژیکی و بیوشیمیایی بذر ذرت(Zea mays L. )در شرایط زوال طبیعی و مصنوعی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 موسسه تحقیقات ثبت و گواهی بذر و نهال

2 دانشیارپژوهش /موسسه تحقیقات ثبت و گواهی بذر و نهال، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران

3 موسسه تحقیقات ثبت و گواهی بذر و نهال، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران

4 کارشناس/ موسسه تحقیقات ثبت و گواهی بذر و نهال، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران

5 موسسه تحقیقات ثبت و گواهی بذر و نهال، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران.

چکیده

این آزمایش، سال 1394در آزمایشگاه مؤسسه‌تحقیقات‌ثبت‌و گواهی‌بذر‌و ‌نهال کرج انجام‌گرفت. تیمارهای‌آزمایشی شامل محل‌-تکثیر‌‌بذر ‌ذرت در دو شرایط ‌اقلیمی کرج و مغان، چگونگی‌شرایط ‌نگهداری‌‌بذر‌ذرت در دو انبارکنترل‌شده و شرایط‌هوای‌آزاد‌منطقه‌-مغان و نحوه‌ ضدعفونی‌بذر در دو‌سطح با تامین شرایط‌ضدعفونی‌ و بدون‌ضدعفونی بودند. آزمایش به‌صورت فاکتوریل‌در‌‌قالب‌طرح-کاملاً‌تصادفی در سه‌تکرار انجام‌‌شد بذرهای‌تکثیری در‌شرایط‌اقلیمی‌کرج قبل از آزمون‌‌پیری‌تسریع‌ شده دارای 92%‌جوانه‌زنی و پس‌از‌-144ساعت در شرایط‌پیری‌‌تسریع‌‌شده، در انبار با شرایط‌کنترل‌‌شده حدود3‌%، وقتی در شرایط‌آب‌و‌هوایی‌مغان نگهداری‌شدند حدود6‌ % افت درصدجوانه‌زنی‌استاندارد نشان‌دادند. در‌حالی‌که شرایط‌مشابه در بذرهای‌تولیدی‌مغان به‌ترتیب منجر‌به‌کاهش18و 28درصدی‌شد. زمانی‌که بذرها در انبار‌کنترل‌شده‌نگهداری‌شدند حدود‌17‌ %‌محتوی‌پروتئین بیش‌تری نسبت به انبارداری در شرایط‌آب‌و‌هوایی‌مغان نشان‌دادند. میزان‌فعالیت‌کاتالاز در بذرهای تولیدی‌کرج از بذرهای تولیدی‌مغان بیش‌تر(14%) بود. ضدعفونی‌بذر بر میزان فعالیت‌آنزیم‌پراکسیداز(p

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