بررسی تغییرات فیزیولوژیکی و بیوشیمیایی بذر ذرت(Zea mays L. )در شرایط زوال طبیعی و مصنوعی

نوع مقاله: مقاله پژوهشی

نویسندگان

1 موسسه تحقیقات ثبت و گواهی بذر و نهال

2 دانشیارپژوهش /موسسه تحقیقات ثبت و گواهی بذر و نهال، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران

3 موسسه تحقیقات ثبت و گواهی بذر و نهال، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران

4 کارشناس/ موسسه تحقیقات ثبت و گواهی بذر و نهال، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران

5 موسسه تحقیقات ثبت و گواهی بذر و نهال، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران.

چکیده

این آزمایش، سال 1394در آزمایشگاه مؤسسه‌تحقیقات‌ثبت‌و گواهی‌بذر‌و ‌نهال کرج انجام‌گرفت. تیمارهای‌آزمایشی شامل محل‌-تکثیر‌‌بذر ‌ذرت در دو شرایط ‌اقلیمی کرج و مغان، چگونگی‌شرایط ‌نگهداری‌‌بذر‌ذرت در دو انبارکنترل‌شده و شرایط‌هوای‌آزاد‌منطقه‌-مغان و نحوه‌ ضدعفونی‌بذر در دو‌سطح با تامین شرایط‌ضدعفونی‌ و بدون‌ضدعفونی بودند. آزمایش به‌صورت فاکتوریل‌در‌‌قالب‌طرح-کاملاً‌تصادفی در سه‌تکرار انجام‌‌شد بذرهای‌تکثیری در‌شرایط‌اقلیمی‌کرج قبل از آزمون‌‌پیری‌تسریع‌ شده دارای 92%‌جوانه‌زنی و پس‌از‌-144ساعت در شرایط‌پیری‌‌تسریع‌‌شده، در انبار با شرایط‌کنترل‌‌شده حدود3‌%، وقتی در شرایط‌آب‌و‌هوایی‌مغان نگهداری‌شدند حدود6‌ % افت درصدجوانه‌زنی‌استاندارد نشان‌دادند. در‌حالی‌که شرایط‌مشابه در بذرهای‌تولیدی‌مغان به‌ترتیب منجر‌به‌کاهش18و 28درصدی‌شد. زمانی‌که بذرها در انبار‌کنترل‌شده‌نگهداری‌شدند حدود‌17‌ %‌محتوی‌پروتئین بیش‌تری نسبت به انبارداری در شرایط‌آب‌و‌هوایی‌مغان نشان‌دادند. میزان‌فعالیت‌کاتالاز در بذرهای تولیدی‌کرج از بذرهای تولیدی‌مغان بیش‌تر(14%) بود. ضدعفونی‌بذر بر میزان فعالیت‌آنزیم‌پراکسیداز(p

کلیدواژه‌ها


Anonymous. 2013. International rules for seed testing. International seed testing association (ISTA), Zurich, Switzerland.

Anonymous. 2013. ISTA Handbook on seeding evaluation. Zurich, Switzerland.

Balesevic-Tubic, S., D. Malenèiæ, M, Tatiæ, and J. Miladinovic. 2005. Influence of aging process on biochemical changes in sunflower seed. HELIA. 28(42): 107-114.

Basra, S.M.A., N. Ahmad, M.M. Khan, Iqbal, N. and M.A. Cheema. 2003. Assessment of cotton seed deterioration during accelerated ageing. Seed Sci. Technol. 31: 531-540.

Bayat, M., and B. Rabiei. 2006. Effect of cold stress and accelerated aging on germination parameters and seedling growth of 5 rape seed cultivars. J. Agric. Sci. Nat. Res. 5:46-57.

Bhutta, A. R., A, Hussain and M, Rafiq-Ur-Rehman. 2004. Handbook on seed processing and storage.

Biabani, A., L.C. Boggs, M. Katozi, and H. Sabouri. 2011. Effects of seed deterioration and inoculation with Mesorhizobium cicerion yield and plant performance of chickpea. Aust. J. Crop. Sci. 5(1): 66-70.

Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein day binding. Anal. Biochem. 72:248-254

Bradley, C.A. 2008. Effect of fungicide seed treatments on stand establishment, seedling disease, and yield of soybean in North Dakota. Plant Disease. 92: 120-125.

Chauhan, D.S., D.P. Deswal, O.S. Dahiya, and R.C. Punia. 2011. Change in storage enzymes activities in natural and accelerated aged seed of wheat (Triticum aestivum Indian J. Agric. Sci. 81 (11): 1037–40.

Chauhan, S.D., P.D. Deswal., S.O. Dahiya., and C.R. Punia. 2011. Change in storage enzymes activities in natural and accelerated aged seed of wheat Indian J. Agric. Sci. 81(11): 1037-40

Davey, M.W., E. Stals, B. Panis, J. Keulemans, and R.L. Swennen. 2005. High-throughput of malondialdehyde in plant tissues. Anal. Biochem. 347: 201-207.

Dehghanshoar, M., A. Hamidi, and S. Mobasser. 2005. Handbook of Vigor Test Methods. Agricultural Education Press.

Delouche, J.C., C.C, Baskin. 1973. Accelerated aging techniques for predicting the relative storability of seed lots. Seed Sci. Technol. 1: 427-452.

Fallon, R.B. 1982. FungicidealFungicidal seed treatment of maize to improve establishment and control seedling patigenspathogens. N. Z. j. Exp. 10: 197-202.

Forcella, F., R.L Benech, Arnold, R. Sanchez, and C.M. Ghersa. 2000. Modeling seedling emergence. Field Crops Res. 67: 123-139.

Goel, A., A.K. Goel, and I.S. Sheoran. 2003. Changes in oxidative stress enzyme during artificial aging in cotton (Gossypium hirsutumL.) seed. J. Plant Physiol. 160: 1093-1100.

Govendera, V., T.A.S. Avelinga, and Q. Kritzingera. 2007.Germination and vigor of maize (Zea mays L.) from northern KwaZulu-Natal and southern Mozambique. SAAB Published by Elsevier B.V.

Heath, R.L. 1987. The biochemistery of ozone attack on the plasma membrane of plant cell. Adv. Phytochem. 21: 29-54.

Hristov, N., N. Mladenov, V. Djuric, A. Kondic-Spika, A, Marjanovic-Jeromela, and D. Simic. 2010. Genotype by environment interactions in wheat quality breeding programs in southeast Europe. Euphytica. 174: 315-324.

Janmohammadi, M., Y. Fallahnezhad, M. Golshan, and H. Mohammadi. 2008. Controlled ageing for storability assessment and predicting seedling early growth of canola cultivars (Brassica napus L.). J. Agric. Biol. Sci. 3(5-6): 22-26.

Kapoor, N., A. Arya., M.A. Siddiqui., H. Kumar., and A. Amir. 2011. Physiology and biochemical changes during seed deterioration in aged seeds of rice (Oryza sativa L.), Am. J. Plant. Physiol. 6(1):28-35

Khatun, A., G. Kabir, and M.A.H. Bhuiyan. 2009. Effect of harvesting stages on the seed quality of lentil (Lens culinaris L.) during storage. Bangladesh Jour. Agril. Res. 34 (4): 565-576.

Loycrajjou, Y., P.C. Lovigny, M. Steven, B. Belghazi, C. Job, and D. Job. 2008. Proteome wide characterization of seed aging in Arabidopsis. A comparison between artificial and natural ageing. Prot. PI. Phy. 148: 620-41.

Ma, F., C. Ewa, M. Tasneem, C.A. Peterson, and M. Gijzen. 2004. Cracks in the palisade cuticle of soybean seed coats correlate with their permeability to water. Ann. Bot. 94: 213-228.

Mahjabin, Bilal, S., and A.B. Abidi. 2015. Physiological and Biochemical changes during Seed Deterioration: A Review. Int. J. Recent Sci. Res (IJRS). 6 (4): 3416-3422.

Marshal, A.H., and D.N. Lewis. 2004. Influence of seed storage conditions on seedling emergence, seedling growth and dry matter production of temperate forage grasses. Seed Sci. Technol. 32: 493-501.

Matthews, S. 2011. Evaluation of early counts of radicle emergence during germination as a repeatable and reproducible vigor test for maize, ISTA Method Validation Reports.

Mc Donald, M.B. 2004. Orthodox seed deterioration and its repair. Pp. 273-304. In R.L. Benech- Arnold and R.L. Sanchez. (eds). Handbook of Seed Physiology. Food Product Press, Argentina.

McDonald, M.B. 1999. Seed deterioration: physiology, repair and assessment. Seed Sci. Technol. 27: 177-237.

Mobasser, S. and J, Rezazade. 2004. Protocol of harvest and process in corn seed. Ministry of Jihad-e-Agriculture, Agricultural Research Education and Extensions Organization (AREEO), Seed and Plant Certification and Registration Institute (SPCRI), (In Persian).

Moles, T.A. and M. Westoby. 2004. Seedling survival and seed size: A synthesis of the literature. J. Ecol. 92: 372–383.

Prochazkova, D., G.C. Sairam, and Srivastava, D.V. 2001. Oxidative stress and antioxidant activity as the basis of senescence in maize leaves. Plant Sci. 161: 765-771.

Schmidt, L. 2000. Guide to handing of tropical and subtropical forest seed. Chapter 8.

Shekaramurthy, S., Patkar, K.l. Shetty, S.A., Praksh, H. S. and Shetty, H. 1994. Effect of thermal treatment on sorghum seed quality in relation to accelerated aging. Seed Sci. Technol. 22 (3):.607-617.

Shelar, V.R. 2008. Role of mechanical damage in deterioration of soybean seed quality during storage – A review. Agric. Rev. 9 (3): 177-184.

Sheligl, H.Q. 1986. Die verwertung orgngischer souren durch chlorella lincht. Planta J. 47-51.

Siami, R. 2010. Corn production technology. Sepehr press.

Soltani, E., B. Galesh I, S. Kamkar and F. Akramghaderi. 2009. The effect of seed aging on wheat emergence on the response of environmental stress. EJCP. 2(2): 43-58.

Talebeni, G.H., and A. Golpayegani.2011. Effect of seed ageing on physiological and biochemical changes in rice seed (Oryza sativa L.), Int. J. Agric. Sc. 1(3):138-143.

Tekrony, D.M. 1995. Accelerated aging. In Van de venter, H.A. (Ed.) Seed vigor testing seminar. ISTA, Copenhagen.

TeKrony, D.M., T. Shande, M. Rucker, and D.B. Egli. 2005. Effect of seed shape on corn germination and vigour during warehouse and controlled environmental storage. Seed Sci. Technol. 33: 185–197.

Tort, N., A.E. Dereboylu, and B. Turkylmaz. 2006. Morphology and physiological effects of fungicide with a thiram agent on some corn culture froms. J. Fac. Sci. 29: 67-79.

Triboi, E., A. Abad, A. Michelena, J. Lloveras, J.L. Ollier, and C. Daniel. 2000. Environmental effects on the quality of two wheat genotypes: 1. quantitative and qualitative variation of storage proteins. Europ. J. Agron. 13: 47-64.

Vashisth, A., and S., Nagarajan, 2009. Germination Characteristics of Seeds of Maize (Zea mays L.) Exposed to Magnetic Fields under Accelerated Ageing Condition, J. Agric Physics. 9:50-58

Verma, S.S., R.P.S. Tomer, and U. Verma. 2003. Loss of viability and vigor in Indian mustard seeds stored under Ambient conditions. Seed Res. 31(1): 98-101.

Verma, S.S., U. Verma, and R.P.S. Tomer. 2003. Studies on seed quality parameters in deteriorating seeds in Brassica (Brassica campestris). Seed Sci. Technol. 31: 389-396.

Wu, X., H. Liu., W. Wang., S. Chen., X. Hu, and C. Li. 2011. Proteomic analysis of seed viability in Maize. Acta Physiol. Planta. 33(1):181-191.

Xin, X., X.H. Lin., Y.C. Zhou., X.L. Chen., X. Liu, and X.X. Lu. 2011. Proteome analysis of Maize seeds: the effect of artificial ageing. Physiol Planta. 143(2):126-138.